Experiment: Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_519 and Pf6N2E2_520 are separated by 10 nucleotides Pf6N2E2_520 and Pf6N2E2_521 are separated by 15 nucleotides Pf6N2E2_521 and Pf6N2E2_522 are separated by 51 nucleotides Pf6N2E2_522 and Pf6N2E2_523 are separated by 77 nucleotides
Pf6N2E2_519 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 589,541 to 590,593
_519
Pf6N2E2_520 - hypothetical protein, at 590,604 to 590,720
_520
Pf6N2E2_521 - hypothetical protein, at 590,736 to 590,888
_521
Pf6N2E2_522 - Inositol transport system sugar-binding protein, at 590,940 to 591,866
_522
Pf6N2E2_523 - Inositol transport system ATP-binding protein, at 591,944 to 593,497
_523
Position (kb) 590
591
592 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 589.998 kb on - strand, within Pf6N2E2_519 at 590.121 kb on - strand, within Pf6N2E2_519 at 590.261 kb on + strand, within Pf6N2E2_519 at 590.262 kb on - strand, within Pf6N2E2_519 at 590.262 kb on - strand, within Pf6N2E2_519 at 590.262 kb on - strand, within Pf6N2E2_519 at 590.429 kb on + strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.724 kb on + strand at 590.724 kb on + strand at 590.724 kb on + strand at 590.724 kb on + strand at 590.725 kb on - strand at 590.729 kb on - strand at 590.763 kb on + strand, within Pf6N2E2_521 at 590.763 kb on + strand, within Pf6N2E2_521 at 590.763 kb on + strand, within Pf6N2E2_521 at 590.763 kb on + strand, within Pf6N2E2_521 at 590.764 kb on - strand, within Pf6N2E2_521 at 590.764 kb on - strand, within Pf6N2E2_521 at 590.907 kb on + strand at 591.060 kb on + strand, within Pf6N2E2_522 at 591.061 kb on - strand, within Pf6N2E2_522 at 591.061 kb on - strand, within Pf6N2E2_522 at 591.478 kb on - strand, within Pf6N2E2_522 at 591.658 kb on + strand, within Pf6N2E2_522 at 591.770 kb on + strand, within Pf6N2E2_522 at 591.770 kb on + strand, within Pf6N2E2_522 at 591.897 kb on - strand at 591.935 kb on + strand at 591.935 kb on + strand at 591.935 kb on + strand at 591.999 kb on + strand at 591.999 kb on + strand at 591.999 kb on + strand at 591.999 kb on + strand at 591.999 kb on + strand at 591.999 kb on + strand at 592.000 kb on - strand at 592.000 kb on - strand at 592.002 kb on + strand at 592.003 kb on - strand at 592.003 kb on - strand at 592.003 kb on - strand at 592.017 kb on - strand at 592.017 kb on - strand at 592.077 kb on + strand at 592.077 kb on + strand at 592.077 kb on + strand at 592.077 kb on + strand at 592.077 kb on + strand at 592.077 kb on + strand at 592.078 kb on - strand at 592.169 kb on + strand, within Pf6N2E2_523 at 592.169 kb on + strand, within Pf6N2E2_523 at 592.170 kb on - strand, within Pf6N2E2_523 at 592.482 kb on - strand, within Pf6N2E2_523 at 592.482 kb on - strand, within Pf6N2E2_523 at 592.482 kb on - strand, within Pf6N2E2_523 at 592.482 kb on - strand, within Pf6N2E2_523 at 592.482 kb on - strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.664 kb on + strand, within Pf6N2E2_523 at 592.664 kb on + strand, within Pf6N2E2_523 at 592.664 kb on + strand, within Pf6N2E2_523 at 592.664 kb on + strand, within Pf6N2E2_523 at 592.664 kb on + strand, within Pf6N2E2_523 at 592.665 kb on - strand, within Pf6N2E2_523 at 592.665 kb on - strand, within Pf6N2E2_523 at 592.665 kb on - strand, within Pf6N2E2_523 at 592.665 kb on - strand, within Pf6N2E2_523 at 592.665 kb on - strand, within Pf6N2E2_523 at 592.665 kb on - strand, within Pf6N2E2_523
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.008 mg/ml remove 589,998 - Pf6N2E2_519 0.43 +0.4 590,121 - Pf6N2E2_519 0.55 -0.3 590,261 + Pf6N2E2_519 0.68 -0.6 590,262 - Pf6N2E2_519 0.68 +0.3 590,262 - Pf6N2E2_519 0.68 +0.7 590,262 - Pf6N2E2_519 0.68 -0.3 590,429 + Pf6N2E2_519 0.84 +1.2 590,430 - Pf6N2E2_519 0.84 -0.3 590,430 - Pf6N2E2_519 0.84 -0.0 590,430 - Pf6N2E2_519 0.84 +0.3 590,430 - Pf6N2E2_519 0.84 -0.1 590,430 - Pf6N2E2_519 0.84 -0.3 590,430 - Pf6N2E2_519 0.84 +0.7 590,724 + +0.2 590,724 + -1.9 590,724 + -0.2 590,724 + -1.2 590,725 - +0.1 590,729 - +0.1 590,763 + Pf6N2E2_521 0.18 -0.2 590,763 + Pf6N2E2_521 0.18 -2.1 590,763 + Pf6N2E2_521 0.18 -0.4 590,763 + Pf6N2E2_521 0.18 +0.3 590,764 - Pf6N2E2_521 0.18 +0.8 590,764 - Pf6N2E2_521 0.18 +0.9 590,907 + +0.1 591,060 + Pf6N2E2_522 0.13 -0.2 591,061 - Pf6N2E2_522 0.13 +0.7 591,061 - Pf6N2E2_522 0.13 -0.9 591,478 - Pf6N2E2_522 0.58 -1.2 591,658 + Pf6N2E2_522 0.77 -0.4 591,770 + Pf6N2E2_522 0.90 -0.9 591,770 + Pf6N2E2_522 0.90 -1.9 591,897 - -0.0 591,935 + +0.1 591,935 + -0.5 591,935 + -0.4 591,999 + -0.5 591,999 + -0.0 591,999 + +0.3 591,999 + -0.6 591,999 + -2.8 591,999 + +0.2 592,000 - -1.6 592,000 - -0.7 592,002 + -0.8 592,003 - -0.6 592,003 - +0.3 592,003 - +0.4 592,017 - +0.2 592,017 - -1.0 592,077 + -0.5 592,077 + -0.3 592,077 + -0.0 592,077 + -1.7 592,077 + +0.5 592,077 + -0.3 592,078 - -0.3 592,169 + Pf6N2E2_523 0.14 +0.2 592,169 + Pf6N2E2_523 0.14 +0.0 592,170 - Pf6N2E2_523 0.15 -0.8 592,482 - Pf6N2E2_523 0.35 -2.1 592,482 - Pf6N2E2_523 0.35 +0.4 592,482 - Pf6N2E2_523 0.35 +0.2 592,482 - Pf6N2E2_523 0.35 -0.2 592,482 - Pf6N2E2_523 0.35 +0.6 592,595 + Pf6N2E2_523 0.42 +0.9 592,595 + Pf6N2E2_523 0.42 +0.2 592,595 + Pf6N2E2_523 0.42 +0.5 592,595 + Pf6N2E2_523 0.42 -0.1 592,595 + Pf6N2E2_523 0.42 +0.0 592,595 + Pf6N2E2_523 0.42 +0.6 592,595 + Pf6N2E2_523 0.42 -3.3 592,595 + Pf6N2E2_523 0.42 +0.1 592,595 + Pf6N2E2_523 0.42 +0.7 592,595 + Pf6N2E2_523 0.42 +0.5 592,664 + Pf6N2E2_523 0.46 +0.7 592,664 + Pf6N2E2_523 0.46 -1.4 592,664 + Pf6N2E2_523 0.46 -0.4 592,664 + Pf6N2E2_523 0.46 -1.2 592,664 + Pf6N2E2_523 0.46 +0.3 592,665 - Pf6N2E2_523 0.46 +0.2 592,665 - Pf6N2E2_523 0.46 +0.3 592,665 - Pf6N2E2_523 0.46 +0.4 592,665 - Pf6N2E2_523 0.46 -0.3 592,665 - Pf6N2E2_523 0.46 +0.5 592,665 - Pf6N2E2_523 0.46 +1.0
Or see this region's nucleotide sequence