Experiment: Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3900 and Pf6N2E2_3901 are separated by 236 nucleotides Pf6N2E2_3901 and Pf6N2E2_3902 are separated by 305 nucleotides Pf6N2E2_3902 and Pf6N2E2_3903 overlap by 4 nucleotides
Pf6N2E2_3900 - 33 kDa chaperonin (Heat shock protein 33) (HSP33), at 4,534,053 to 4,534,955
_3900
Pf6N2E2_3901 - Ribosome-associated heat shock protein implicated in the recycling of the 50S subunit (S4 paralog), at 4,535,192 to 4,535,596
_3901
Pf6N2E2_3902 - Ribosomal protein S6 glutaminyl transferase, at 4,535,902 to 4,536,315
_3902
Pf6N2E2_3903 - Ribosomal protein S6 glutaminyl transferase, at 4,536,312 to 4,537,217
_3903
Position (kb)
4535
4536 Strain fitness (log2 ratio)
-1
0
1
2 at 4534.956 kb on - strand at 4534.966 kb on - strand at 4535.000 kb on - strand at 4535.000 kb on - strand at 4535.000 kb on - strand at 4535.338 kb on - strand, within Pf6N2E2_3901 at 4535.530 kb on - strand, within Pf6N2E2_3901 at 4535.620 kb on - strand at 4535.620 kb on - strand at 4535.620 kb on - strand at 4535.631 kb on + strand at 4535.631 kb on + strand at 4535.631 kb on + strand at 4535.631 kb on + strand at 4535.631 kb on + strand at 4535.632 kb on - strand at 4535.672 kb on + strand at 4535.673 kb on - strand at 4535.673 kb on - strand at 4535.673 kb on - strand at 4535.713 kb on - strand at 4535.743 kb on + strand at 4535.744 kb on - strand at 4535.747 kb on + strand at 4535.747 kb on + strand at 4535.747 kb on + strand at 4535.747 kb on + strand at 4535.748 kb on - strand at 4535.748 kb on - strand at 4535.748 kb on - strand at 4535.748 kb on - strand at 4535.748 kb on - strand at 4535.748 kb on - strand at 4535.748 kb on - strand at 4535.748 kb on - strand at 4535.768 kb on + strand at 4535.769 kb on - strand at 4535.769 kb on - strand at 4535.812 kb on + strand at 4535.812 kb on + strand at 4535.812 kb on + strand at 4535.813 kb on - strand at 4535.813 kb on - strand at 4535.832 kb on - strand at 4536.078 kb on - strand, within Pf6N2E2_3902 at 4536.109 kb on + strand, within Pf6N2E2_3902 at 4536.110 kb on - strand, within Pf6N2E2_3902 at 4536.256 kb on + strand, within Pf6N2E2_3902 at 4536.256 kb on + strand, within Pf6N2E2_3902 at 4536.256 kb on + strand, within Pf6N2E2_3902 at 4536.257 kb on - strand, within Pf6N2E2_3902 at 4536.257 kb on - strand, within Pf6N2E2_3902 at 4536.257 kb on - strand, within Pf6N2E2_3902 at 4536.257 kb on - strand, within Pf6N2E2_3902 at 4536.291 kb on + strand at 4536.291 kb on + strand at 4536.291 kb on + strand at 4536.348 kb on + strand at 4536.348 kb on + strand at 4536.348 kb on + strand at 4536.349 kb on - strand at 4536.423 kb on + strand, within Pf6N2E2_3903 at 4536.423 kb on + strand, within Pf6N2E2_3903 at 4536.525 kb on + strand, within Pf6N2E2_3903 at 4536.525 kb on + strand, within Pf6N2E2_3903 at 4536.525 kb on + strand, within Pf6N2E2_3903 at 4536.525 kb on + strand, within Pf6N2E2_3903 at 4536.525 kb on + strand, within Pf6N2E2_3903
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.008 mg/ml remove 4,534,956 - -0.1 4,534,966 - +0.9 4,535,000 - -0.8 4,535,000 - +0.5 4,535,000 - +1.0 4,535,338 - Pf6N2E2_3901 0.36 -1.3 4,535,530 - Pf6N2E2_3901 0.83 -0.5 4,535,620 - -1.5 4,535,620 - -0.1 4,535,620 - +0.3 4,535,631 + -0.9 4,535,631 + +0.1 4,535,631 + +0.0 4,535,631 + -1.0 4,535,631 + -0.3 4,535,632 - -0.6 4,535,672 + +0.6 4,535,673 - +1.2 4,535,673 - -0.5 4,535,673 - -0.0 4,535,713 - -0.5 4,535,743 + -0.3 4,535,744 - -0.9 4,535,747 + +0.6 4,535,747 + +0.6 4,535,747 + +0.3 4,535,747 + +0.1 4,535,748 - -0.7 4,535,748 - -0.3 4,535,748 - +0.4 4,535,748 - -0.0 4,535,748 - -0.4 4,535,748 - +0.5 4,535,748 - +0.2 4,535,748 - -0.1 4,535,768 + +0.5 4,535,769 - +1.8 4,535,769 - -0.3 4,535,812 + +1.1 4,535,812 + +0.9 4,535,812 + -1.7 4,535,813 - +0.5 4,535,813 - +0.6 4,535,832 - -0.3 4,536,078 - Pf6N2E2_3902 0.43 -0.1 4,536,109 + Pf6N2E2_3902 0.50 +0.8 4,536,110 - Pf6N2E2_3902 0.50 -0.2 4,536,256 + Pf6N2E2_3902 0.86 -1.2 4,536,256 + Pf6N2E2_3902 0.86 -0.3 4,536,256 + Pf6N2E2_3902 0.86 +0.4 4,536,257 - Pf6N2E2_3902 0.86 +0.4 4,536,257 - Pf6N2E2_3902 0.86 +0.1 4,536,257 - Pf6N2E2_3902 0.86 +0.4 4,536,257 - Pf6N2E2_3902 0.86 -0.9 4,536,291 + +0.5 4,536,291 + +0.4 4,536,291 + +0.5 4,536,348 + -0.9 4,536,348 + +0.3 4,536,348 + -0.5 4,536,349 - +0.4 4,536,423 + Pf6N2E2_3903 0.12 +1.0 4,536,423 + Pf6N2E2_3903 0.12 +0.7 4,536,525 + Pf6N2E2_3903 0.24 -0.2 4,536,525 + Pf6N2E2_3903 0.24 +0.4 4,536,525 + Pf6N2E2_3903 0.24 -0.9 4,536,525 + Pf6N2E2_3903 0.24 +0.2 4,536,525 + Pf6N2E2_3903 0.24 -0.7
Or see this region's nucleotide sequence