Experiment: Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3128 and Pf6N2E2_3129 are separated by 1 nucleotides Pf6N2E2_3129 and Pf6N2E2_3130 are separated by 25 nucleotides Pf6N2E2_3130 and Pf6N2E2_3131 are separated by 72 nucleotides
Pf6N2E2_3128 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 3,619,634 to 3,620,671
_3128
Pf6N2E2_3129 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 3,620,673 to 3,621,620
_3129
Pf6N2E2_3130 - Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1), at 3,621,646 to 3,622,428
_3130
Pf6N2E2_3131 - 4-aminobutyraldehyde dehydrogenase (EC 1.2.1.19), at 3,622,501 to 3,623,925
_3131
Position (kb)
3621
3622
3623 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3620.760 kb on + strand at 3620.761 kb on - strand at 3620.761 kb on - strand at 3620.761 kb on - strand at 3620.808 kb on + strand, within Pf6N2E2_3129 at 3620.809 kb on - strand, within Pf6N2E2_3129 at 3620.847 kb on + strand, within Pf6N2E2_3129 at 3620.848 kb on - strand, within Pf6N2E2_3129 at 3621.006 kb on + strand, within Pf6N2E2_3129 at 3621.006 kb on + strand, within Pf6N2E2_3129 at 3621.006 kb on + strand, within Pf6N2E2_3129 at 3621.007 kb on - strand, within Pf6N2E2_3129 at 3621.007 kb on - strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.022 kb on - strand, within Pf6N2E2_3129 at 3621.051 kb on + strand, within Pf6N2E2_3129 at 3621.051 kb on + strand, within Pf6N2E2_3129 at 3621.052 kb on - strand, within Pf6N2E2_3129 at 3621.084 kb on + strand, within Pf6N2E2_3129 at 3621.084 kb on + strand, within Pf6N2E2_3129 at 3621.085 kb on - strand, within Pf6N2E2_3129 at 3621.085 kb on - strand, within Pf6N2E2_3129 at 3621.085 kb on - strand, within Pf6N2E2_3129 at 3621.085 kb on - strand, within Pf6N2E2_3129 at 3621.099 kb on + strand, within Pf6N2E2_3129 at 3621.100 kb on - strand, within Pf6N2E2_3129 at 3621.100 kb on - strand, within Pf6N2E2_3129 at 3621.100 kb on - strand, within Pf6N2E2_3129 at 3621.100 kb on - strand, within Pf6N2E2_3129 at 3621.255 kb on + strand, within Pf6N2E2_3129 at 3621.256 kb on - strand, within Pf6N2E2_3129 at 3621.256 kb on - strand, within Pf6N2E2_3129 at 3621.477 kb on + strand, within Pf6N2E2_3129 at 3621.477 kb on + strand, within Pf6N2E2_3129 at 3621.478 kb on - strand, within Pf6N2E2_3129 at 3621.478 kb on - strand, within Pf6N2E2_3129 at 3621.499 kb on - strand, within Pf6N2E2_3129 at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.577 kb on - strand at 3621.577 kb on - strand at 3621.577 kb on - strand at 3621.580 kb on + strand at 3621.580 kb on + strand at 3621.580 kb on + strand at 3621.581 kb on - strand at 3621.581 kb on - strand at 3621.709 kb on + strand at 3621.919 kb on - strand, within Pf6N2E2_3130 at 3622.063 kb on + strand, within Pf6N2E2_3130 at 3622.157 kb on - strand, within Pf6N2E2_3130 at 3622.349 kb on + strand, within Pf6N2E2_3130 at 3622.349 kb on + strand, within Pf6N2E2_3130 at 3622.350 kb on - strand at 3622.378 kb on + strand at 3622.378 kb on + strand at 3622.379 kb on - strand at 3622.379 kb on - strand at 3622.381 kb on + strand at 3622.381 kb on + strand at 3622.381 kb on + strand at 3622.426 kb on + strand at 3622.426 kb on + strand at 3622.426 kb on + strand at 3622.427 kb on - strand at 3622.427 kb on - strand at 3622.876 kb on + strand, within Pf6N2E2_3131 at 3622.948 kb on + strand, within Pf6N2E2_3131 at 3622.948 kb on + strand, within Pf6N2E2_3131 at 3622.949 kb on - strand, within Pf6N2E2_3131 at 3623.001 kb on + strand, within Pf6N2E2_3131 at 3623.087 kb on + strand, within Pf6N2E2_3131 at 3623.088 kb on - strand, within Pf6N2E2_3131 at 3623.088 kb on - strand, within Pf6N2E2_3131 at 3623.320 kb on + strand, within Pf6N2E2_3131 at 3623.321 kb on - strand, within Pf6N2E2_3131 at 3623.359 kb on + strand, within Pf6N2E2_3131 at 3623.360 kb on - strand, within Pf6N2E2_3131 at 3623.360 kb on - strand, within Pf6N2E2_3131 at 3623.360 kb on - strand, within Pf6N2E2_3131 at 3623.377 kb on + strand, within Pf6N2E2_3131 at 3623.377 kb on + strand, within Pf6N2E2_3131 at 3623.378 kb on - strand, within Pf6N2E2_3131 at 3623.378 kb on - strand, within Pf6N2E2_3131 at 3623.425 kb on + strand, within Pf6N2E2_3131 at 3623.425 kb on + strand, within Pf6N2E2_3131 at 3623.425 kb on + strand, within Pf6N2E2_3131 at 3623.426 kb on - strand, within Pf6N2E2_3131
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.008 mg/ml remove 3,620,760 + -0.3 3,620,761 - -0.5 3,620,761 - -0.6 3,620,761 - +0.6 3,620,808 + Pf6N2E2_3129 0.14 +0.2 3,620,809 - Pf6N2E2_3129 0.14 -1.1 3,620,847 + Pf6N2E2_3129 0.18 -0.7 3,620,848 - Pf6N2E2_3129 0.18 -1.2 3,621,006 + Pf6N2E2_3129 0.35 +0.3 3,621,006 + Pf6N2E2_3129 0.35 -0.5 3,621,006 + Pf6N2E2_3129 0.35 +0.2 3,621,007 - Pf6N2E2_3129 0.35 +0.3 3,621,007 - Pf6N2E2_3129 0.35 -0.2 3,621,009 + Pf6N2E2_3129 0.35 -0.1 3,621,009 + Pf6N2E2_3129 0.35 -0.8 3,621,009 + Pf6N2E2_3129 0.35 +0.8 3,621,009 + Pf6N2E2_3129 0.35 +1.0 3,621,009 + Pf6N2E2_3129 0.35 +0.0 3,621,009 + Pf6N2E2_3129 0.35 +0.5 3,621,009 + Pf6N2E2_3129 0.35 +0.8 3,621,009 + Pf6N2E2_3129 0.35 -0.3 3,621,009 + Pf6N2E2_3129 0.35 -0.6 3,621,010 - Pf6N2E2_3129 0.36 -0.8 3,621,010 - Pf6N2E2_3129 0.36 +0.8 3,621,010 - Pf6N2E2_3129 0.36 +1.0 3,621,010 - Pf6N2E2_3129 0.36 +0.5 3,621,010 - Pf6N2E2_3129 0.36 -0.3 3,621,010 - Pf6N2E2_3129 0.36 -0.5 3,621,010 - Pf6N2E2_3129 0.36 -0.6 3,621,010 - Pf6N2E2_3129 0.36 +0.5 3,621,010 - Pf6N2E2_3129 0.36 -1.5 3,621,010 - Pf6N2E2_3129 0.36 +0.4 3,621,022 - Pf6N2E2_3129 0.37 +0.6 3,621,051 + Pf6N2E2_3129 0.40 -0.1 3,621,051 + Pf6N2E2_3129 0.40 +0.2 3,621,052 - Pf6N2E2_3129 0.40 -0.1 3,621,084 + Pf6N2E2_3129 0.43 -0.3 3,621,084 + Pf6N2E2_3129 0.43 -1.7 3,621,085 - Pf6N2E2_3129 0.43 -0.9 3,621,085 - Pf6N2E2_3129 0.43 -0.7 3,621,085 - Pf6N2E2_3129 0.43 -0.9 3,621,085 - Pf6N2E2_3129 0.43 +0.5 3,621,099 + Pf6N2E2_3129 0.45 +0.1 3,621,100 - Pf6N2E2_3129 0.45 +0.6 3,621,100 - Pf6N2E2_3129 0.45 +0.9 3,621,100 - Pf6N2E2_3129 0.45 -0.6 3,621,100 - Pf6N2E2_3129 0.45 -0.5 3,621,255 + Pf6N2E2_3129 0.61 +0.7 3,621,256 - Pf6N2E2_3129 0.61 +0.1 3,621,256 - Pf6N2E2_3129 0.61 -0.5 3,621,477 + Pf6N2E2_3129 0.85 +2.2 3,621,477 + Pf6N2E2_3129 0.85 -1.1 3,621,478 - Pf6N2E2_3129 0.85 -1.3 3,621,478 - Pf6N2E2_3129 0.85 +0.0 3,621,499 - Pf6N2E2_3129 0.87 -0.6 3,621,576 + +0.2 3,621,576 + -0.8 3,621,576 + -0.2 3,621,576 + -0.5 3,621,576 + +0.8 3,621,577 - -0.5 3,621,577 - +0.5 3,621,577 - +0.6 3,621,580 + +1.4 3,621,580 + +0.5 3,621,580 + +0.1 3,621,581 - +0.5 3,621,581 - +0.9 3,621,709 + -0.4 3,621,919 - Pf6N2E2_3130 0.35 -1.7 3,622,063 + Pf6N2E2_3130 0.53 -0.6 3,622,157 - Pf6N2E2_3130 0.65 -0.4 3,622,349 + Pf6N2E2_3130 0.90 -0.8 3,622,349 + Pf6N2E2_3130 0.90 -0.1 3,622,350 - -0.6 3,622,378 + -0.7 3,622,378 + +0.6 3,622,379 - -1.4 3,622,379 - +0.2 3,622,381 + +1.2 3,622,381 + -0.5 3,622,381 + -0.7 3,622,426 + -0.2 3,622,426 + +0.3 3,622,426 + +1.1 3,622,427 - +0.2 3,622,427 - +0.1 3,622,876 + Pf6N2E2_3131 0.26 +0.6 3,622,948 + Pf6N2E2_3131 0.31 -2.0 3,622,948 + Pf6N2E2_3131 0.31 +0.4 3,622,949 - Pf6N2E2_3131 0.31 -0.9 3,623,001 + Pf6N2E2_3131 0.35 -0.5 3,623,087 + Pf6N2E2_3131 0.41 +0.6 3,623,088 - Pf6N2E2_3131 0.41 +0.3 3,623,088 - Pf6N2E2_3131 0.41 +0.2 3,623,320 + Pf6N2E2_3131 0.57 +1.0 3,623,321 - Pf6N2E2_3131 0.58 -0.2 3,623,359 + Pf6N2E2_3131 0.60 -0.6 3,623,360 - Pf6N2E2_3131 0.60 +0.9 3,623,360 - Pf6N2E2_3131 0.60 -0.0 3,623,360 - Pf6N2E2_3131 0.60 +0.7 3,623,377 + Pf6N2E2_3131 0.61 +0.7 3,623,377 + Pf6N2E2_3131 0.61 -0.0 3,623,378 - Pf6N2E2_3131 0.62 +0.7 3,623,378 - Pf6N2E2_3131 0.62 +0.7 3,623,425 + Pf6N2E2_3131 0.65 +0.3 3,623,425 + Pf6N2E2_3131 0.65 -0.5 3,623,425 + Pf6N2E2_3131 0.65 -0.7 3,623,426 - Pf6N2E2_3131 0.65 +0.2
Or see this region's nucleotide sequence