Experiment: Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3033 and Pf6N2E2_3034 are separated by 311 nucleotides Pf6N2E2_3034 and Pf6N2E2_3035 are separated by 27 nucleotides Pf6N2E2_3035 and Pf6N2E2_3036 are separated by 142 nucleotides Pf6N2E2_3036 and Pf6N2E2_3037 are separated by 489 nucleotides
Pf6N2E2_3033 - hypothetical protein, at 3,517,482 to 3,517,649
_3033
Pf6N2E2_3034 - hypothetical protein, at 3,517,961 to 3,518,116
_3034
Pf6N2E2_3035 - DNA mismatch repair protein MutS, at 3,518,144 to 3,520,723
_3035
Pf6N2E2_3036 - 4Fe-4S ferredoxin, iron-sulfur binding, at 3,520,866 to 3,521,189
_3036
Pf6N2E2_3037 - RNA polymerase sigma factor RpoS, at 3,521,679 to 3,522,686
_3037
Position (kb)
3518
3519
3520
3521 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3517.173 kb on - strand at 3517.173 kb on - strand at 3517.173 kb on - strand at 3517.173 kb on - strand at 3517.243 kb on - strand at 3517.281 kb on - strand at 3517.284 kb on - strand at 3517.284 kb on - strand at 3517.284 kb on - strand at 3517.320 kb on + strand at 3517.320 kb on + strand at 3517.320 kb on + strand at 3517.324 kb on + strand at 3517.324 kb on + strand at 3517.324 kb on + strand at 3517.324 kb on + strand at 3517.324 kb on + strand at 3517.324 kb on + strand at 3517.481 kb on + strand at 3517.586 kb on - strand, within Pf6N2E2_3033 at 3517.586 kb on - strand, within Pf6N2E2_3033 at 3517.636 kb on + strand at 3517.683 kb on + strand at 3517.684 kb on - strand at 3517.842 kb on + strand at 3517.842 kb on + strand at 3517.843 kb on - strand at 3517.843 kb on - strand at 3517.843 kb on - strand at 3517.843 kb on - strand at 3517.843 kb on - strand at 3517.932 kb on - strand at 3517.932 kb on - strand at 3517.992 kb on + strand, within Pf6N2E2_3034 at 3517.992 kb on + strand, within Pf6N2E2_3034 at 3517.992 kb on + strand, within Pf6N2E2_3034 at 3517.992 kb on + strand, within Pf6N2E2_3034 at 3517.992 kb on + strand, within Pf6N2E2_3034 at 3517.992 kb on + strand, within Pf6N2E2_3034 at 3517.993 kb on - strand, within Pf6N2E2_3034 at 3517.993 kb on - strand, within Pf6N2E2_3034 at 3517.993 kb on - strand, within Pf6N2E2_3034 at 3517.993 kb on - strand, within Pf6N2E2_3034 at 3517.993 kb on - strand, within Pf6N2E2_3034 at 3517.996 kb on + strand, within Pf6N2E2_3034 at 3517.996 kb on + strand, within Pf6N2E2_3034 at 3517.997 kb on - strand, within Pf6N2E2_3034 at 3517.997 kb on - strand, within Pf6N2E2_3034 at 3518.018 kb on + strand, within Pf6N2E2_3034 at 3518.116 kb on + strand at 3518.116 kb on + strand at 3518.116 kb on + strand at 3518.150 kb on - strand at 3518.195 kb on + strand at 3518.196 kb on - strand at 3518.196 kb on - strand at 3518.256 kb on - strand at 3518.256 kb on - strand at 3518.256 kb on - strand at 3518.267 kb on + strand at 3518.268 kb on - strand at 3518.268 kb on - strand at 3518.363 kb on + strand at 3518.364 kb on - strand at 3518.382 kb on - strand at 3518.498 kb on - strand, within Pf6N2E2_3035 at 3518.874 kb on - strand, within Pf6N2E2_3035 at 3519.130 kb on + strand, within Pf6N2E2_3035 at 3519.130 kb on + strand, within Pf6N2E2_3035 at 3519.131 kb on - strand, within Pf6N2E2_3035 at 3519.131 kb on - strand, within Pf6N2E2_3035 at 3519.131 kb on - strand, within Pf6N2E2_3035 at 3519.351 kb on - strand, within Pf6N2E2_3035 at 3519.459 kb on + strand, within Pf6N2E2_3035 at 3519.459 kb on + strand, within Pf6N2E2_3035 at 3519.459 kb on + strand, within Pf6N2E2_3035 at 3519.460 kb on - strand, within Pf6N2E2_3035 at 3519.460 kb on - strand, within Pf6N2E2_3035 at 3519.460 kb on - strand, within Pf6N2E2_3035 at 3519.551 kb on + strand, within Pf6N2E2_3035 at 3519.551 kb on + strand, within Pf6N2E2_3035 at 3519.552 kb on - strand, within Pf6N2E2_3035 at 3519.569 kb on + strand, within Pf6N2E2_3035 at 3519.569 kb on + strand, within Pf6N2E2_3035 at 3519.617 kb on + strand, within Pf6N2E2_3035 at 3519.617 kb on + strand, within Pf6N2E2_3035 at 3519.617 kb on + strand, within Pf6N2E2_3035 at 3519.617 kb on + strand, within Pf6N2E2_3035 at 3519.617 kb on + strand, within Pf6N2E2_3035 at 3519.618 kb on - strand, within Pf6N2E2_3035 at 3519.618 kb on - strand, within Pf6N2E2_3035 at 3519.734 kb on + strand, within Pf6N2E2_3035 at 3519.734 kb on + strand, within Pf6N2E2_3035 at 3519.735 kb on - strand, within Pf6N2E2_3035 at 3519.735 kb on - strand, within Pf6N2E2_3035 at 3519.735 kb on - strand, within Pf6N2E2_3035 at 3519.735 kb on - strand, within Pf6N2E2_3035 at 3519.924 kb on + strand, within Pf6N2E2_3035 at 3519.924 kb on + strand, within Pf6N2E2_3035 at 3519.924 kb on + strand, within Pf6N2E2_3035 at 3519.925 kb on - strand, within Pf6N2E2_3035 at 3520.009 kb on + strand, within Pf6N2E2_3035 at 3520.010 kb on - strand, within Pf6N2E2_3035 at 3520.020 kb on - strand, within Pf6N2E2_3035 at 3520.020 kb on - strand, within Pf6N2E2_3035 at 3520.064 kb on - strand, within Pf6N2E2_3035 at 3520.064 kb on - strand, within Pf6N2E2_3035 at 3520.092 kb on + strand, within Pf6N2E2_3035 at 3520.253 kb on - strand, within Pf6N2E2_3035 at 3520.314 kb on - strand, within Pf6N2E2_3035 at 3520.314 kb on - strand, within Pf6N2E2_3035 at 3520.334 kb on + strand, within Pf6N2E2_3035 at 3520.695 kb on + strand at 3520.700 kb on + strand at 3520.702 kb on + strand at 3520.702 kb on + strand at 3520.702 kb on + strand at 3520.702 kb on + strand at 3520.702 kb on + strand at 3520.702 kb on + strand at 3520.702 kb on + strand at 3520.702 kb on + strand at 3520.702 kb on + strand at 3520.702 kb on + strand at 3520.703 kb on - strand at 3520.703 kb on - strand at 3520.721 kb on + strand at 3520.721 kb on + strand at 3520.721 kb on + strand at 3520.721 kb on + strand at 3520.721 kb on + strand at 3520.721 kb on + strand at 3520.721 kb on + strand at 3520.721 kb on + strand at 3520.721 kb on + strand at 3520.721 kb on + strand at 3520.722 kb on - strand at 3520.722 kb on - strand at 3520.726 kb on + strand at 3520.726 kb on + strand at 3520.726 kb on + strand at 3520.726 kb on + strand at 3520.776 kb on + strand at 3520.779 kb on + strand at 3520.779 kb on + strand at 3520.779 kb on + strand at 3521.399 kb on + strand at 3521.400 kb on - strand at 3521.400 kb on - strand at 3521.400 kb on - strand at 3521.402 kb on + strand at 3521.402 kb on + strand at 3521.402 kb on + strand at 3521.403 kb on - strand at 3521.403 kb on - strand at 3521.405 kb on + strand at 3521.405 kb on + strand at 3521.436 kb on - strand at 3521.436 kb on - strand at 3521.493 kb on + strand at 3521.517 kb on + strand at 3521.517 kb on + strand at 3521.518 kb on - strand at 3521.518 kb on - strand at 3521.518 kb on - strand at 3521.518 kb on - strand at 3521.519 kb on + strand at 3521.555 kb on - strand at 3521.564 kb on + strand at 3521.576 kb on + strand at 3521.576 kb on + strand at 3521.576 kb on + strand at 3521.577 kb on - strand at 3521.577 kb on - strand at 3521.577 kb on - strand at 3521.589 kb on + strand at 3521.590 kb on - strand at 3521.596 kb on - strand at 3521.596 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.008 mg/ml remove 3,517,173 - +0.0 3,517,173 - +0.2 3,517,173 - -0.1 3,517,173 - +0.3 3,517,243 - +0.6 3,517,281 - -0.2 3,517,284 - +0.1 3,517,284 - -0.0 3,517,284 - -0.3 3,517,320 + -0.8 3,517,320 + -0.2 3,517,320 + -1.0 3,517,324 + +0.0 3,517,324 + -0.4 3,517,324 + -0.7 3,517,324 + -0.4 3,517,324 + +0.7 3,517,324 + -0.1 3,517,481 + -0.0 3,517,586 - Pf6N2E2_3033 0.62 +1.3 3,517,586 - Pf6N2E2_3033 0.62 +0.7 3,517,636 + -0.9 3,517,683 + +0.1 3,517,684 - +1.1 3,517,842 + +0.6 3,517,842 + +0.7 3,517,843 - +0.3 3,517,843 - +0.6 3,517,843 - +0.3 3,517,843 - +0.7 3,517,843 - +0.8 3,517,932 - -0.1 3,517,932 - +0.6 3,517,992 + Pf6N2E2_3034 0.20 +0.4 3,517,992 + Pf6N2E2_3034 0.20 +0.0 3,517,992 + Pf6N2E2_3034 0.20 +0.5 3,517,992 + Pf6N2E2_3034 0.20 -0.8 3,517,992 + Pf6N2E2_3034 0.20 +0.3 3,517,992 + Pf6N2E2_3034 0.20 +0.0 3,517,993 - Pf6N2E2_3034 0.21 -1.3 3,517,993 - Pf6N2E2_3034 0.21 +0.6 3,517,993 - Pf6N2E2_3034 0.21 +0.0 3,517,993 - Pf6N2E2_3034 0.21 +0.2 3,517,993 - Pf6N2E2_3034 0.21 +0.5 3,517,996 + Pf6N2E2_3034 0.22 -2.0 3,517,996 + Pf6N2E2_3034 0.22 +0.8 3,517,997 - Pf6N2E2_3034 0.23 +0.3 3,517,997 - Pf6N2E2_3034 0.23 +0.1 3,518,018 + Pf6N2E2_3034 0.37 +0.2 3,518,116 + -0.3 3,518,116 + -0.3 3,518,116 + -0.2 3,518,150 - -0.2 3,518,195 + +0.8 3,518,196 - -1.2 3,518,196 - +0.4 3,518,256 - +0.0 3,518,256 - +0.7 3,518,256 - +0.6 3,518,267 + +1.0 3,518,268 - +1.3 3,518,268 - +0.0 3,518,363 + +0.1 3,518,364 - -1.6 3,518,382 - +0.1 3,518,498 - Pf6N2E2_3035 0.14 +0.2 3,518,874 - Pf6N2E2_3035 0.28 +1.3 3,519,130 + Pf6N2E2_3035 0.38 +0.4 3,519,130 + Pf6N2E2_3035 0.38 +0.9 3,519,131 - Pf6N2E2_3035 0.38 -0.7 3,519,131 - Pf6N2E2_3035 0.38 +0.7 3,519,131 - Pf6N2E2_3035 0.38 -0.2 3,519,351 - Pf6N2E2_3035 0.47 -0.1 3,519,459 + Pf6N2E2_3035 0.51 +2.2 3,519,459 + Pf6N2E2_3035 0.51 +0.0 3,519,459 + Pf6N2E2_3035 0.51 -0.1 3,519,460 - Pf6N2E2_3035 0.51 -0.0 3,519,460 - Pf6N2E2_3035 0.51 +0.4 3,519,460 - Pf6N2E2_3035 0.51 +0.3 3,519,551 + Pf6N2E2_3035 0.55 +1.3 3,519,551 + Pf6N2E2_3035 0.55 -1.3 3,519,552 - Pf6N2E2_3035 0.55 -0.0 3,519,569 + Pf6N2E2_3035 0.55 +0.5 3,519,569 + Pf6N2E2_3035 0.55 +0.3 3,519,617 + Pf6N2E2_3035 0.57 +0.2 3,519,617 + Pf6N2E2_3035 0.57 +1.1 3,519,617 + Pf6N2E2_3035 0.57 +0.2 3,519,617 + Pf6N2E2_3035 0.57 +1.2 3,519,617 + Pf6N2E2_3035 0.57 -1.2 3,519,618 - Pf6N2E2_3035 0.57 +1.4 3,519,618 - Pf6N2E2_3035 0.57 +1.3 3,519,734 + Pf6N2E2_3035 0.62 +0.2 3,519,734 + Pf6N2E2_3035 0.62 +0.6 3,519,735 - Pf6N2E2_3035 0.62 -0.2 3,519,735 - Pf6N2E2_3035 0.62 -1.0 3,519,735 - Pf6N2E2_3035 0.62 +1.0 3,519,735 - Pf6N2E2_3035 0.62 +1.8 3,519,924 + Pf6N2E2_3035 0.69 -0.0 3,519,924 + Pf6N2E2_3035 0.69 +0.9 3,519,924 + Pf6N2E2_3035 0.69 -0.6 3,519,925 - Pf6N2E2_3035 0.69 +0.9 3,520,009 + Pf6N2E2_3035 0.72 +1.0 3,520,010 - Pf6N2E2_3035 0.72 +0.7 3,520,020 - Pf6N2E2_3035 0.73 -0.4 3,520,020 - Pf6N2E2_3035 0.73 +0.8 3,520,064 - Pf6N2E2_3035 0.74 -0.1 3,520,064 - Pf6N2E2_3035 0.74 +0.4 3,520,092 + Pf6N2E2_3035 0.76 +0.2 3,520,253 - Pf6N2E2_3035 0.82 +0.1 3,520,314 - Pf6N2E2_3035 0.84 -0.4 3,520,314 - Pf6N2E2_3035 0.84 +0.5 3,520,334 + Pf6N2E2_3035 0.85 -0.9 3,520,695 + +0.6 3,520,700 + +0.6 3,520,702 + -0.0 3,520,702 + +0.0 3,520,702 + +0.3 3,520,702 + +1.0 3,520,702 + +1.1 3,520,702 + -1.1 3,520,702 + +1.1 3,520,702 + +0.1 3,520,702 + +2.0 3,520,702 + +0.0 3,520,703 - +1.1 3,520,703 - +1.4 3,520,721 + -0.9 3,520,721 + +1.7 3,520,721 + +0.4 3,520,721 + -0.7 3,520,721 + +0.4 3,520,721 + +0.9 3,520,721 + -1.2 3,520,721 + +0.2 3,520,721 + -0.9 3,520,721 + +1.4 3,520,722 - +1.5 3,520,722 - -1.6 3,520,726 + +0.8 3,520,726 + -0.7 3,520,726 + +0.5 3,520,726 + +0.4 3,520,776 + +0.6 3,520,779 + +0.6 3,520,779 + -0.4 3,520,779 + +0.2 3,521,399 + +0.3 3,521,400 - -1.6 3,521,400 - +0.3 3,521,400 - -0.1 3,521,402 + +0.2 3,521,402 + +0.4 3,521,402 + -0.5 3,521,403 - -0.4 3,521,403 - -0.2 3,521,405 + -0.4 3,521,405 + +0.3 3,521,436 - +1.5 3,521,436 - -1.4 3,521,493 + +0.3 3,521,517 + +0.8 3,521,517 + +0.8 3,521,518 - -0.6 3,521,518 - +0.7 3,521,518 - +0.3 3,521,518 - +1.1 3,521,519 + -0.2 3,521,555 - -2.7 3,521,564 + -0.3 3,521,576 + -2.5 3,521,576 + -1.1 3,521,576 + -0.4 3,521,577 - -1.3 3,521,577 - +0.8 3,521,577 - +0.4 3,521,589 + -0.4 3,521,590 - +0.0 3,521,596 - +0.8 3,521,596 - +0.9
Or see this region's nucleotide sequence