Experiment: Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2600 and Pf6N2E2_2601 are separated by 82 nucleotides Pf6N2E2_2601 and Pf6N2E2_2602 are separated by 66 nucleotides
Pf6N2E2_2600 - Flagellar hook-associated protein FliD, at 3,057,526 to 3,058,959
_2600
Pf6N2E2_2601 - Flagellin protein FlaG, at 3,059,042 to 3,059,413
_2601
Pf6N2E2_2602 - Flagellin protein FlaA, at 3,059,480 to 3,060,331
_2602
Position (kb)
3059
3060 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 3058.111 kb on - strand, within Pf6N2E2_2600 at 3058.111 kb on - strand, within Pf6N2E2_2600 at 3058.111 kb on - strand, within Pf6N2E2_2600 at 3058.111 kb on - strand, within Pf6N2E2_2600 at 3058.111 kb on - strand, within Pf6N2E2_2600 at 3058.111 kb on - strand, within Pf6N2E2_2600 at 3058.111 kb on - strand, within Pf6N2E2_2600 at 3058.111 kb on - strand, within Pf6N2E2_2600 at 3058.111 kb on - strand, within Pf6N2E2_2600 at 3058.111 kb on - strand, within Pf6N2E2_2600 at 3058.112 kb on + strand, within Pf6N2E2_2600 at 3058.112 kb on + strand, within Pf6N2E2_2600 at 3058.112 kb on + strand, within Pf6N2E2_2600 at 3058.112 kb on + strand, within Pf6N2E2_2600 at 3058.112 kb on + strand, within Pf6N2E2_2600 at 3058.112 kb on + strand, within Pf6N2E2_2600 at 3058.113 kb on - strand, within Pf6N2E2_2600 at 3058.113 kb on - strand, within Pf6N2E2_2600 at 3058.113 kb on - strand, within Pf6N2E2_2600 at 3058.113 kb on - strand, within Pf6N2E2_2600 at 3058.113 kb on - strand, within Pf6N2E2_2600 at 3058.113 kb on - strand, within Pf6N2E2_2600 at 3058.224 kb on + strand, within Pf6N2E2_2600 at 3058.224 kb on + strand, within Pf6N2E2_2600 at 3058.224 kb on + strand, within Pf6N2E2_2600 at 3058.225 kb on - strand, within Pf6N2E2_2600 at 3058.225 kb on - strand, within Pf6N2E2_2600 at 3058.225 kb on - strand, within Pf6N2E2_2600 at 3058.225 kb on - strand, within Pf6N2E2_2600 at 3058.225 kb on - strand, within Pf6N2E2_2600 at 3058.225 kb on - strand, within Pf6N2E2_2600 at 3058.225 kb on - strand, within Pf6N2E2_2600 at 3058.225 kb on - strand, within Pf6N2E2_2600 at 3058.323 kb on + strand, within Pf6N2E2_2600 at 3058.324 kb on - strand, within Pf6N2E2_2600 at 3058.383 kb on + strand, within Pf6N2E2_2600 at 3058.384 kb on - strand, within Pf6N2E2_2600 at 3058.384 kb on - strand, within Pf6N2E2_2600 at 3058.384 kb on - strand, within Pf6N2E2_2600 at 3058.475 kb on + strand, within Pf6N2E2_2600 at 3058.475 kb on + strand, within Pf6N2E2_2600 at 3058.475 kb on + strand, within Pf6N2E2_2600 at 3058.475 kb on + strand, within Pf6N2E2_2600 at 3058.475 kb on + strand, within Pf6N2E2_2600 at 3058.476 kb on - strand, within Pf6N2E2_2600 at 3058.476 kb on - strand, within Pf6N2E2_2600 at 3058.476 kb on - strand, within Pf6N2E2_2600 at 3058.476 kb on - strand, within Pf6N2E2_2600 at 3058.476 kb on - strand, within Pf6N2E2_2600 at 3058.476 kb on - strand, within Pf6N2E2_2600 at 3058.476 kb on - strand, within Pf6N2E2_2600 at 3058.476 kb on - strand, within Pf6N2E2_2600 at 3058.482 kb on + strand, within Pf6N2E2_2600 at 3058.482 kb on + strand, within Pf6N2E2_2600 at 3058.483 kb on - strand, within Pf6N2E2_2600 at 3058.508 kb on - strand, within Pf6N2E2_2600 at 3058.508 kb on - strand, within Pf6N2E2_2600 at 3058.528 kb on - strand, within Pf6N2E2_2600 at 3058.539 kb on - strand, within Pf6N2E2_2600 at 3058.545 kb on + strand, within Pf6N2E2_2600 at 3058.545 kb on + strand, within Pf6N2E2_2600 at 3058.580 kb on - strand, within Pf6N2E2_2600 at 3058.658 kb on + strand, within Pf6N2E2_2600 at 3058.658 kb on + strand, within Pf6N2E2_2600 at 3058.658 kb on + strand, within Pf6N2E2_2600 at 3058.658 kb on + strand, within Pf6N2E2_2600 at 3058.659 kb on - strand, within Pf6N2E2_2600 at 3058.734 kb on + strand, within Pf6N2E2_2600 at 3058.821 kb on + strand at 3058.822 kb on - strand at 3058.903 kb on - strand at 3058.928 kb on - strand at 3058.942 kb on + strand at 3059.000 kb on - strand at 3059.000 kb on - strand at 3059.000 kb on - strand at 3059.019 kb on - strand at 3059.019 kb on - strand at 3059.019 kb on - strand at 3059.087 kb on - strand, within Pf6N2E2_2601 at 3059.159 kb on - strand, within Pf6N2E2_2601 at 3059.165 kb on + strand, within Pf6N2E2_2601 at 3059.165 kb on + strand at 3059.166 kb on - strand, within Pf6N2E2_2601 at 3059.166 kb on - strand, within Pf6N2E2_2601 at 3059.166 kb on - strand, within Pf6N2E2_2601 at 3059.188 kb on + strand, within Pf6N2E2_2601 at 3059.188 kb on + strand, within Pf6N2E2_2601 at 3059.189 kb on - strand, within Pf6N2E2_2601 at 3059.189 kb on - strand, within Pf6N2E2_2601 at 3059.189 kb on - strand, within Pf6N2E2_2601 at 3059.304 kb on - strand, within Pf6N2E2_2601 at 3059.304 kb on - strand, within Pf6N2E2_2601 at 3059.304 kb on - strand, within Pf6N2E2_2601 at 3059.402 kb on - strand at 3059.443 kb on - strand at 3059.478 kb on + strand at 3059.478 kb on + strand at 3059.479 kb on - strand at 3059.479 kb on - strand at 3059.479 kb on - strand at 3059.479 kb on - strand at 3059.479 kb on - strand at 3059.479 kb on - strand at 3059.481 kb on + strand at 3059.481 kb on + strand at 3059.482 kb on - strand at 3059.482 kb on - strand at 3059.482 kb on - strand at 3059.498 kb on + strand at 3059.498 kb on + strand at 3059.499 kb on - strand at 3059.606 kb on + strand, within Pf6N2E2_2602 at 3059.607 kb on - strand, within Pf6N2E2_2602 at 3059.607 kb on - strand, within Pf6N2E2_2602 at 3059.607 kb on - strand, within Pf6N2E2_2602 at 3059.666 kb on - strand, within Pf6N2E2_2602 at 3059.666 kb on - strand, within Pf6N2E2_2602 at 3059.899 kb on + strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3060.059 kb on - strand, within Pf6N2E2_2602 at 3060.059 kb on - strand, within Pf6N2E2_2602 at 3060.059 kb on - strand, within Pf6N2E2_2602 at 3060.118 kb on + strand, within Pf6N2E2_2602 at 3060.118 kb on + strand, within Pf6N2E2_2602 at 3060.119 kb on - strand, within Pf6N2E2_2602 at 3060.119 kb on - strand, within Pf6N2E2_2602 at 3060.119 kb on - strand, within Pf6N2E2_2602 at 3060.172 kb on + strand, within Pf6N2E2_2602 at 3060.173 kb on - strand, within Pf6N2E2_2602 at 3060.317 kb on + strand at 3060.323 kb on + strand at 3060.393 kb on + strand at 3060.394 kb on - strand at 3060.407 kb on - strand at 3060.409 kb on + strand at 3060.409 kb on + strand at 3060.409 kb on + strand at 3060.410 kb on - strand at 3060.410 kb on - strand at 3060.410 kb on - strand at 3060.413 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.008 mg/ml remove 3,058,111 - Pf6N2E2_2600 0.41 -0.2 3,058,111 - Pf6N2E2_2600 0.41 +1.5 3,058,111 - Pf6N2E2_2600 0.41 +1.2 3,058,111 - Pf6N2E2_2600 0.41 +0.6 3,058,111 - Pf6N2E2_2600 0.41 -0.2 3,058,111 - Pf6N2E2_2600 0.41 -0.0 3,058,111 - Pf6N2E2_2600 0.41 +2.0 3,058,111 - Pf6N2E2_2600 0.41 +0.7 3,058,111 - Pf6N2E2_2600 0.41 +1.1 3,058,111 - Pf6N2E2_2600 0.41 +1.0 3,058,112 + Pf6N2E2_2600 0.41 +0.6 3,058,112 + Pf6N2E2_2600 0.41 +0.9 3,058,112 + Pf6N2E2_2600 0.41 +0.1 3,058,112 + Pf6N2E2_2600 0.41 +0.4 3,058,112 + Pf6N2E2_2600 0.41 +0.3 3,058,112 + Pf6N2E2_2600 0.41 +1.0 3,058,113 - Pf6N2E2_2600 0.41 +0.7 3,058,113 - Pf6N2E2_2600 0.41 +0.8 3,058,113 - Pf6N2E2_2600 0.41 -0.0 3,058,113 - Pf6N2E2_2600 0.41 +0.6 3,058,113 - Pf6N2E2_2600 0.41 -0.0 3,058,113 - Pf6N2E2_2600 0.41 +0.4 3,058,224 + Pf6N2E2_2600 0.49 +0.2 3,058,224 + Pf6N2E2_2600 0.49 +1.0 3,058,224 + Pf6N2E2_2600 0.49 +1.0 3,058,225 - Pf6N2E2_2600 0.49 +1.3 3,058,225 - Pf6N2E2_2600 0.49 +1.3 3,058,225 - Pf6N2E2_2600 0.49 -0.0 3,058,225 - Pf6N2E2_2600 0.49 +0.8 3,058,225 - Pf6N2E2_2600 0.49 +0.7 3,058,225 - Pf6N2E2_2600 0.49 -0.8 3,058,225 - Pf6N2E2_2600 0.49 +0.9 3,058,225 - Pf6N2E2_2600 0.49 +0.2 3,058,323 + Pf6N2E2_2600 0.56 +1.7 3,058,324 - Pf6N2E2_2600 0.56 +1.9 3,058,383 + Pf6N2E2_2600 0.60 +0.8 3,058,384 - Pf6N2E2_2600 0.60 -0.3 3,058,384 - Pf6N2E2_2600 0.60 +1.3 3,058,384 - Pf6N2E2_2600 0.60 +0.9 3,058,475 + Pf6N2E2_2600 0.66 +1.0 3,058,475 + Pf6N2E2_2600 0.66 +1.9 3,058,475 + Pf6N2E2_2600 0.66 +0.8 3,058,475 + Pf6N2E2_2600 0.66 +1.5 3,058,475 + Pf6N2E2_2600 0.66 +0.5 3,058,476 - Pf6N2E2_2600 0.66 +1.4 3,058,476 - Pf6N2E2_2600 0.66 +1.1 3,058,476 - Pf6N2E2_2600 0.66 +0.7 3,058,476 - Pf6N2E2_2600 0.66 +1.6 3,058,476 - Pf6N2E2_2600 0.66 +1.4 3,058,476 - Pf6N2E2_2600 0.66 -0.6 3,058,476 - Pf6N2E2_2600 0.66 -0.0 3,058,476 - Pf6N2E2_2600 0.66 +0.2 3,058,482 + Pf6N2E2_2600 0.67 +0.8 3,058,482 + Pf6N2E2_2600 0.67 +0.8 3,058,483 - Pf6N2E2_2600 0.67 +1.1 3,058,508 - Pf6N2E2_2600 0.68 +0.5 3,058,508 - Pf6N2E2_2600 0.68 +1.2 3,058,528 - Pf6N2E2_2600 0.70 -0.4 3,058,539 - Pf6N2E2_2600 0.71 +0.2 3,058,545 + Pf6N2E2_2600 0.71 -0.2 3,058,545 + Pf6N2E2_2600 0.71 -1.0 3,058,580 - Pf6N2E2_2600 0.74 +0.1 3,058,658 + Pf6N2E2_2600 0.79 +0.6 3,058,658 + Pf6N2E2_2600 0.79 +0.5 3,058,658 + Pf6N2E2_2600 0.79 +1.0 3,058,658 + Pf6N2E2_2600 0.79 +1.3 3,058,659 - Pf6N2E2_2600 0.79 -0.2 3,058,734 + Pf6N2E2_2600 0.84 +0.3 3,058,821 + -0.1 3,058,822 - +0.5 3,058,903 - -0.1 3,058,928 - +0.4 3,058,942 + +0.6 3,059,000 - -0.5 3,059,000 - +0.4 3,059,000 - +0.7 3,059,019 - +0.4 3,059,019 - -0.8 3,059,019 - +0.3 3,059,087 - Pf6N2E2_2601 0.12 -0.7 3,059,159 - Pf6N2E2_2601 0.31 -1.3 3,059,165 + Pf6N2E2_2601 0.33 -0.2 3,059,165 + +3.9 3,059,166 - Pf6N2E2_2601 0.33 +0.9 3,059,166 - Pf6N2E2_2601 0.33 -0.9 3,059,166 - Pf6N2E2_2601 0.33 -0.1 3,059,188 + Pf6N2E2_2601 0.39 -0.6 3,059,188 + Pf6N2E2_2601 0.39 -1.0 3,059,189 - Pf6N2E2_2601 0.40 +0.0 3,059,189 - Pf6N2E2_2601 0.40 +0.1 3,059,189 - Pf6N2E2_2601 0.40 -1.1 3,059,304 - Pf6N2E2_2601 0.70 -1.3 3,059,304 - Pf6N2E2_2601 0.70 +0.6 3,059,304 - Pf6N2E2_2601 0.70 +0.3 3,059,402 - -0.3 3,059,443 - +0.8 3,059,478 + -0.2 3,059,478 + -0.6 3,059,479 - -1.2 3,059,479 - +0.9 3,059,479 - +0.7 3,059,479 - +0.4 3,059,479 - +0.6 3,059,479 - +1.1 3,059,481 + +1.0 3,059,481 + +0.4 3,059,482 - +1.1 3,059,482 - -0.6 3,059,482 - +2.0 3,059,498 + -0.1 3,059,498 + +0.9 3,059,499 - +0.5 3,059,606 + Pf6N2E2_2602 0.15 +1.3 3,059,607 - Pf6N2E2_2602 0.15 +0.1 3,059,607 - Pf6N2E2_2602 0.15 +1.1 3,059,607 - Pf6N2E2_2602 0.15 +0.6 3,059,666 - Pf6N2E2_2602 0.22 +0.8 3,059,666 - Pf6N2E2_2602 0.22 -0.2 3,059,899 + Pf6N2E2_2602 0.49 +0.3 3,059,900 - Pf6N2E2_2602 0.49 +0.7 3,059,900 - Pf6N2E2_2602 0.49 +0.9 3,059,900 - Pf6N2E2_2602 0.49 +0.6 3,059,900 - Pf6N2E2_2602 0.49 +0.8 3,059,900 - Pf6N2E2_2602 0.49 +0.5 3,059,900 - Pf6N2E2_2602 0.49 +0.4 3,060,059 - Pf6N2E2_2602 0.68 -0.8 3,060,059 - Pf6N2E2_2602 0.68 +1.0 3,060,059 - Pf6N2E2_2602 0.68 +1.3 3,060,118 + Pf6N2E2_2602 0.75 +0.5 3,060,118 + Pf6N2E2_2602 0.75 +1.1 3,060,119 - Pf6N2E2_2602 0.75 +0.7 3,060,119 - Pf6N2E2_2602 0.75 +1.8 3,060,119 - Pf6N2E2_2602 0.75 +0.8 3,060,172 + Pf6N2E2_2602 0.81 +0.8 3,060,173 - Pf6N2E2_2602 0.81 +0.8 3,060,317 + +0.3 3,060,323 + +1.9 3,060,393 + +0.6 3,060,394 - +0.1 3,060,407 - +0.4 3,060,409 + +0.4 3,060,409 + +0.3 3,060,409 + +0.5 3,060,410 - -0.6 3,060,410 - +0.3 3,060,410 - -1.2 3,060,413 + -0.1
Or see this region's nucleotide sequence