Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_194

Experiment: Nalidixic 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_192 and Pf6N2E2_193 are separated by 377 nucleotidesPf6N2E2_193 and Pf6N2E2_194 overlap by 36 nucleotidesPf6N2E2_194 and Pf6N2E2_195 overlap by 4 nucleotides Pf6N2E2_192 - hypothetical protein, at 244,676 to 246,115 _192 Pf6N2E2_193 - hypothetical protein, at 246,493 to 246,870 _193 Pf6N2E2_194 - Alcohol dehydrogenase (EC 1.1.1.1), at 246,835 to 247,569 _194 Pf6N2E2_195 - hypothetical protein, at 247,566 to 249,515 _195 Position (kb) 246 247 248Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 245.861 kb on + strand, within Pf6N2E2_192at 246.091 kb on - strandat 246.091 kb on - strandat 246.173 kb on + strandat 246.173 kb on + strandat 246.174 kb on - strandat 246.174 kb on - strandat 246.249 kb on - strandat 246.253 kb on + strandat 246.254 kb on - strandat 246.254 kb on - strandat 246.254 kb on - strandat 246.254 kb on - strandat 246.286 kb on + strandat 246.286 kb on + strandat 246.304 kb on + strandat 246.304 kb on + strandat 246.304 kb on + strandat 246.305 kb on - strandat 246.305 kb on - strandat 246.305 kb on - strandat 246.305 kb on - strandat 246.305 kb on - strandat 246.353 kb on - strandat 246.462 kb on + strandat 246.465 kb on + strandat 246.465 kb on + strandat 246.465 kb on + strandat 246.498 kb on + strandat 246.498 kb on + strandat 246.500 kb on + strandat 246.511 kb on + strandat 246.511 kb on + strandat 246.512 kb on - strandat 246.517 kb on - strandat 246.539 kb on + strand, within Pf6N2E2_193at 246.539 kb on + strand, within Pf6N2E2_193at 246.539 kb on + strand, within Pf6N2E2_193at 246.544 kb on + strand, within Pf6N2E2_193at 246.544 kb on + strand, within Pf6N2E2_193at 246.772 kb on - strand, within Pf6N2E2_193at 246.889 kb on + strandat 246.889 kb on + strandat 246.889 kb on + strandat 246.890 kb on - strandat 247.116 kb on + strand, within Pf6N2E2_194at 247.117 kb on - strand, within Pf6N2E2_194at 247.205 kb on + strand, within Pf6N2E2_194at 247.215 kb on + strand, within Pf6N2E2_194at 247.216 kb on - strand, within Pf6N2E2_194at 247.376 kb on - strand, within Pf6N2E2_194at 247.376 kb on - strand, within Pf6N2E2_194at 247.376 kb on - strand, within Pf6N2E2_194at 247.376 kb on - strand, within Pf6N2E2_194at 247.645 kb on + strandat 247.652 kb on - strandat 247.652 kb on - strandat 247.751 kb on + strandat 247.861 kb on + strand, within Pf6N2E2_195at 247.861 kb on + strand, within Pf6N2E2_195at 247.861 kb on + strand, within Pf6N2E2_195at 247.861 kb on + strand, within Pf6N2E2_195at 247.861 kb on + strand, within Pf6N2E2_195at 247.861 kb on + strand, within Pf6N2E2_195at 247.861 kb on + strand, within Pf6N2E2_195at 247.861 kb on + strand, within Pf6N2E2_195at 247.861 kb on + strand, within Pf6N2E2_195at 247.861 kb on + strand, within Pf6N2E2_195at 247.862 kb on - strand, within Pf6N2E2_195at 247.862 kb on - strand, within Pf6N2E2_195at 247.862 kb on - strand, within Pf6N2E2_195at 247.862 kb on - strand, within Pf6N2E2_195at 247.893 kb on + strand, within Pf6N2E2_195at 247.893 kb on + strand, within Pf6N2E2_195at 247.915 kb on + strand, within Pf6N2E2_195at 247.916 kb on - strand, within Pf6N2E2_195at 247.918 kb on + strand, within Pf6N2E2_195at 247.918 kb on + strand, within Pf6N2E2_195at 247.918 kb on + strand, within Pf6N2E2_195at 247.918 kb on + strand, within Pf6N2E2_195at 247.919 kb on - strand, within Pf6N2E2_195at 247.921 kb on + strand, within Pf6N2E2_195at 247.921 kb on + strand, within Pf6N2E2_195at 247.921 kb on + strand, within Pf6N2E2_195at 247.922 kb on - strand, within Pf6N2E2_195at 247.922 kb on - strand, within Pf6N2E2_195at 247.986 kb on - strand, within Pf6N2E2_195at 248.008 kb on + strand, within Pf6N2E2_195at 248.050 kb on + strand, within Pf6N2E2_195at 248.106 kb on + strand, within Pf6N2E2_195at 248.140 kb on + strand, within Pf6N2E2_195at 248.227 kb on + strand, within Pf6N2E2_195at 248.227 kb on + strand, within Pf6N2E2_195at 248.228 kb on - strand, within Pf6N2E2_195at 248.228 kb on - strand, within Pf6N2E2_195at 248.268 kb on + strand, within Pf6N2E2_195at 248.362 kb on + strand, within Pf6N2E2_195at 248.362 kb on + strand, within Pf6N2E2_195at 248.363 kb on - strand, within Pf6N2E2_195at 248.363 kb on - strand, within Pf6N2E2_195at 248.363 kb on - strand, within Pf6N2E2_195at 248.449 kb on + strand, within Pf6N2E2_195at 248.450 kb on - strand, within Pf6N2E2_195at 248.524 kb on + strand, within Pf6N2E2_195

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Per-strain Table

Position Strand Gene LocusTag Fraction Nalidixic 0.008 mg/ml
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245,861 + Pf6N2E2_192 0.82 +0.7
246,091 - -0.7
246,091 - -0.6
246,173 + -0.3
246,173 + +0.0
246,174 - +0.1
246,174 - -0.2
246,249 - -0.6
246,253 + -0.0
246,254 - +0.4
246,254 - +0.3
246,254 - +0.1
246,254 - +0.5
246,286 + -0.4
246,286 + +0.9
246,304 + -2.4
246,304 + +0.6
246,304 + -0.1
246,305 - +0.1
246,305 - +1.8
246,305 - -0.1
246,305 - +0.5
246,305 - -0.2
246,353 - +0.4
246,462 + -0.5
246,465 + -0.6
246,465 + -0.9
246,465 + -0.6
246,498 + +0.4
246,498 + -0.6
246,500 + +0.5
246,511 + +0.4
246,511 + +0.3
246,512 - -0.7
246,517 - -0.2
246,539 + Pf6N2E2_193 0.12 +0.1
246,539 + Pf6N2E2_193 0.12 -0.2
246,539 + Pf6N2E2_193 0.12 -1.3
246,544 + Pf6N2E2_193 0.13 +0.3
246,544 + Pf6N2E2_193 0.13 -0.2
246,772 - Pf6N2E2_193 0.74 -1.5
246,889 + -1.0
246,889 + -0.2
246,889 + +3.0
246,890 - +0.8
247,116 + Pf6N2E2_194 0.38 -0.0
247,117 - Pf6N2E2_194 0.38 +0.0
247,205 + Pf6N2E2_194 0.50 +0.7
247,215 + Pf6N2E2_194 0.52 +0.9
247,216 - Pf6N2E2_194 0.52 +1.0
247,376 - Pf6N2E2_194 0.74 -1.0
247,376 - Pf6N2E2_194 0.74 +0.9
247,376 - Pf6N2E2_194 0.74 -0.6
247,376 - Pf6N2E2_194 0.74 +0.9
247,645 + +0.2
247,652 - +0.3
247,652 - -0.9
247,751 + +0.5
247,861 + Pf6N2E2_195 0.15 +1.0
247,861 + Pf6N2E2_195 0.15 +0.6
247,861 + Pf6N2E2_195 0.15 -2.7
247,861 + Pf6N2E2_195 0.15 +0.2
247,861 + Pf6N2E2_195 0.15 -0.6
247,861 + Pf6N2E2_195 0.15 +0.4
247,861 + Pf6N2E2_195 0.15 +0.1
247,861 + Pf6N2E2_195 0.15 +0.7
247,861 + Pf6N2E2_195 0.15 -0.2
247,861 + Pf6N2E2_195 0.15 -0.6
247,862 - Pf6N2E2_195 0.15 -0.3
247,862 - Pf6N2E2_195 0.15 +0.9
247,862 - Pf6N2E2_195 0.15 -0.7
247,862 - Pf6N2E2_195 0.15 -0.1
247,893 + Pf6N2E2_195 0.17 -2.6
247,893 + Pf6N2E2_195 0.17 -0.6
247,915 + Pf6N2E2_195 0.18 +0.4
247,916 - Pf6N2E2_195 0.18 +0.0
247,918 + Pf6N2E2_195 0.18 -0.6
247,918 + Pf6N2E2_195 0.18 -0.1
247,918 + Pf6N2E2_195 0.18 -0.3
247,918 + Pf6N2E2_195 0.18 -0.1
247,919 - Pf6N2E2_195 0.18 -0.4
247,921 + Pf6N2E2_195 0.18 -1.6
247,921 + Pf6N2E2_195 0.18 +1.4
247,921 + Pf6N2E2_195 0.18 +0.3
247,922 - Pf6N2E2_195 0.18 +1.0
247,922 - Pf6N2E2_195 0.18 -0.2
247,986 - Pf6N2E2_195 0.22 -0.8
248,008 + Pf6N2E2_195 0.23 -0.2
248,050 + Pf6N2E2_195 0.25 -0.6
248,106 + Pf6N2E2_195 0.28 -3.5
248,140 + Pf6N2E2_195 0.29 -1.4
248,227 + Pf6N2E2_195 0.34 +0.2
248,227 + Pf6N2E2_195 0.34 -1.0
248,228 - Pf6N2E2_195 0.34 +0.1
248,228 - Pf6N2E2_195 0.34 -0.0
248,268 + Pf6N2E2_195 0.36 -1.2
248,362 + Pf6N2E2_195 0.41 +1.1
248,362 + Pf6N2E2_195 0.41 -1.1
248,363 - Pf6N2E2_195 0.41 -0.7
248,363 - Pf6N2E2_195 0.41 -0.5
248,363 - Pf6N2E2_195 0.41 -0.5
248,449 + Pf6N2E2_195 0.45 -1.3
248,450 - Pf6N2E2_195 0.45 -0.6
248,524 + Pf6N2E2_195 0.49 -1.1

Or see this region's nucleotide sequence