Experiment: Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1611 and Pf6N2E2_1612 overlap by 4 nucleotides Pf6N2E2_1612 and Pf6N2E2_1613 are separated by 186 nucleotides Pf6N2E2_1613 and Pf6N2E2_1614 are separated by 221 nucleotides
Pf6N2E2_1611 - Phosphonate ABC transporter permease protein phnE2 (TC 3.A.1.9.1), at 1,884,982 to 1,885,809
_1611
Pf6N2E2_1612 - Phosphonate ABC transporter permease protein phnE1 (TC 3.A.1.9.1), at 1,885,806 to 1,886,573
_1612
Pf6N2E2_1613 - short-chain dehydrogenase/reductase, at 1,886,760 to 1,887,503
_1613
Pf6N2E2_1614 - Transcriptional regulator, LysR family, at 1,887,725 to 1,888,630
_1614
Position (kb)
1886
1887
1888 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1885.932 kb on + strand, within Pf6N2E2_1612 at 1885.933 kb on - strand, within Pf6N2E2_1612 at 1886.057 kb on - strand, within Pf6N2E2_1612 at 1886.367 kb on + strand, within Pf6N2E2_1612 at 1886.367 kb on + strand, within Pf6N2E2_1612 at 1886.367 kb on + strand, within Pf6N2E2_1612 at 1886.367 kb on + strand, within Pf6N2E2_1612 at 1886.368 kb on - strand, within Pf6N2E2_1612 at 1886.368 kb on - strand, within Pf6N2E2_1612 at 1886.451 kb on + strand, within Pf6N2E2_1612 at 1886.451 kb on + strand, within Pf6N2E2_1612 at 1886.451 kb on + strand, within Pf6N2E2_1612 at 1886.451 kb on + strand, within Pf6N2E2_1612 at 1886.609 kb on - strand at 1886.698 kb on + strand at 1886.745 kb on + strand at 1886.766 kb on - strand at 1886.787 kb on - strand at 1886.791 kb on + strand at 1887.201 kb on + strand, within Pf6N2E2_1613 at 1887.201 kb on + strand, within Pf6N2E2_1613 at 1887.202 kb on - strand, within Pf6N2E2_1613 at 1887.202 kb on - strand, within Pf6N2E2_1613 at 1887.202 kb on - strand, within Pf6N2E2_1613 at 1887.460 kb on - strand at 1887.460 kb on - strand at 1887.489 kb on + strand at 1887.489 kb on + strand at 1887.489 kb on + strand at 1887.491 kb on + strand at 1887.492 kb on - strand at 1887.492 kb on - strand at 1887.492 kb on - strand at 1887.521 kb on + strand at 1887.521 kb on + strand at 1887.522 kb on - strand at 1887.533 kb on + strand at 1887.533 kb on + strand at 1887.533 kb on + strand at 1887.534 kb on - strand at 1887.551 kb on + strand at 1887.551 kb on + strand at 1887.551 kb on + strand at 1887.591 kb on + strand at 1887.879 kb on - strand, within Pf6N2E2_1614 at 1887.879 kb on - strand, within Pf6N2E2_1614 at 1887.965 kb on + strand, within Pf6N2E2_1614 at 1887.965 kb on + strand, within Pf6N2E2_1614 at 1887.966 kb on - strand, within Pf6N2E2_1614 at 1887.969 kb on + strand, within Pf6N2E2_1614 at 1887.970 kb on - strand, within Pf6N2E2_1614 at 1887.970 kb on - strand, within Pf6N2E2_1614 at 1887.970 kb on - strand, within Pf6N2E2_1614 at 1888.208 kb on - strand, within Pf6N2E2_1614 at 1888.336 kb on + strand, within Pf6N2E2_1614 at 1888.452 kb on + strand, within Pf6N2E2_1614 at 1888.453 kb on - strand, within Pf6N2E2_1614 at 1888.456 kb on - strand, within Pf6N2E2_1614
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.008 mg/ml remove 1,885,932 + Pf6N2E2_1612 0.16 -0.5 1,885,933 - Pf6N2E2_1612 0.17 -0.4 1,886,057 - Pf6N2E2_1612 0.33 -1.2 1,886,367 + Pf6N2E2_1612 0.73 +0.6 1,886,367 + Pf6N2E2_1612 0.73 +2.4 1,886,367 + Pf6N2E2_1612 0.73 -0.1 1,886,367 + Pf6N2E2_1612 0.73 -1.2 1,886,368 - Pf6N2E2_1612 0.73 -1.8 1,886,368 - Pf6N2E2_1612 0.73 -0.0 1,886,451 + Pf6N2E2_1612 0.84 -0.2 1,886,451 + Pf6N2E2_1612 0.84 +1.3 1,886,451 + Pf6N2E2_1612 0.84 +1.4 1,886,451 + Pf6N2E2_1612 0.84 +0.6 1,886,609 - -0.1 1,886,698 + -0.1 1,886,745 + +1.2 1,886,766 - -0.5 1,886,787 - +2.9 1,886,791 + +0.4 1,887,201 + Pf6N2E2_1613 0.59 -0.9 1,887,201 + Pf6N2E2_1613 0.59 -0.2 1,887,202 - Pf6N2E2_1613 0.59 -0.8 1,887,202 - Pf6N2E2_1613 0.59 -1.7 1,887,202 - Pf6N2E2_1613 0.59 +0.0 1,887,460 - -0.1 1,887,460 - +0.5 1,887,489 + +0.2 1,887,489 + -0.7 1,887,489 + -0.6 1,887,491 + +0.1 1,887,492 - -0.4 1,887,492 - +0.8 1,887,492 - +0.2 1,887,521 + +1.0 1,887,521 + +0.1 1,887,522 - -0.4 1,887,533 + -0.4 1,887,533 + -0.0 1,887,533 + +0.2 1,887,534 - -0.6 1,887,551 + +0.0 1,887,551 + -0.1 1,887,551 + -0.9 1,887,591 + -0.7 1,887,879 - Pf6N2E2_1614 0.17 -0.8 1,887,879 - Pf6N2E2_1614 0.17 -0.2 1,887,965 + Pf6N2E2_1614 0.26 +0.5 1,887,965 + Pf6N2E2_1614 0.26 -1.6 1,887,966 - Pf6N2E2_1614 0.27 -0.5 1,887,969 + Pf6N2E2_1614 0.27 +0.4 1,887,970 - Pf6N2E2_1614 0.27 -0.7 1,887,970 - Pf6N2E2_1614 0.27 -0.1 1,887,970 - Pf6N2E2_1614 0.27 +0.5 1,888,208 - Pf6N2E2_1614 0.53 +0.6 1,888,336 + Pf6N2E2_1614 0.67 +1.1 1,888,452 + Pf6N2E2_1614 0.80 +0.4 1,888,453 - Pf6N2E2_1614 0.80 -1.1 1,888,456 - Pf6N2E2_1614 0.81 +0.4
Or see this region's nucleotide sequence