Experiment: Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1456 and Pf6N2E2_1457 are separated by 0 nucleotides Pf6N2E2_1457 and Pf6N2E2_1458 are separated by 10 nucleotides Pf6N2E2_1458 and Pf6N2E2_1459 are separated by 106 nucleotides
Pf6N2E2_1456 - D-xylose transport ATP-binding protein XylG, at 1,704,446 to 1,706,002
_1456
Pf6N2E2_1457 - Xylose ABC transporter, permease protein XylH, at 1,706,003 to 1,707,139
_1457
Pf6N2E2_1458 - probable transporter, LysE family, at 1,707,150 to 1,707,758
_1458
Pf6N2E2_1459 - Glycine cleavage system transcriptional activator GcvA, at 1,707,865 to 1,708,770
_1459
Position (kb)
1706
1707
1708 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1705.043 kb on + strand, within Pf6N2E2_1456 at 1705.043 kb on + strand, within Pf6N2E2_1456 at 1705.044 kb on - strand, within Pf6N2E2_1456 at 1705.044 kb on - strand, within Pf6N2E2_1456 at 1705.253 kb on + strand, within Pf6N2E2_1456 at 1705.556 kb on - strand, within Pf6N2E2_1456 at 1705.559 kb on + strand, within Pf6N2E2_1456 at 1705.559 kb on + strand, within Pf6N2E2_1456 at 1705.776 kb on - strand, within Pf6N2E2_1456 at 1705.776 kb on - strand, within Pf6N2E2_1456 at 1705.784 kb on + strand, within Pf6N2E2_1456 at 1705.784 kb on + strand, within Pf6N2E2_1456 at 1705.784 kb on + strand, within Pf6N2E2_1456 at 1705.785 kb on - strand, within Pf6N2E2_1456 at 1705.785 kb on - strand, within Pf6N2E2_1456 at 1705.982 kb on - strand at 1705.982 kb on - strand at 1706.351 kb on + strand, within Pf6N2E2_1457 at 1706.351 kb on + strand, within Pf6N2E2_1457 at 1706.352 kb on - strand, within Pf6N2E2_1457 at 1706.466 kb on - strand, within Pf6N2E2_1457 at 1706.480 kb on + strand, within Pf6N2E2_1457 at 1706.480 kb on + strand, within Pf6N2E2_1457 at 1706.608 kb on - strand, within Pf6N2E2_1457 at 1706.651 kb on + strand, within Pf6N2E2_1457 at 1706.651 kb on + strand, within Pf6N2E2_1457 at 1706.654 kb on - strand, within Pf6N2E2_1457 at 1706.715 kb on + strand, within Pf6N2E2_1457 at 1706.716 kb on - strand, within Pf6N2E2_1457 at 1706.723 kb on + strand, within Pf6N2E2_1457 at 1706.808 kb on + strand, within Pf6N2E2_1457 at 1706.808 kb on + strand, within Pf6N2E2_1457 at 1706.808 kb on + strand, within Pf6N2E2_1457 at 1706.809 kb on - strand, within Pf6N2E2_1457 at 1706.987 kb on + strand, within Pf6N2E2_1457 at 1706.987 kb on + strand, within Pf6N2E2_1457 at 1706.988 kb on - strand, within Pf6N2E2_1457 at 1706.988 kb on - strand, within Pf6N2E2_1457 at 1707.060 kb on - strand at 1707.144 kb on + strand at 1707.144 kb on + strand at 1707.305 kb on + strand, within Pf6N2E2_1458 at 1707.307 kb on + strand, within Pf6N2E2_1458 at 1707.307 kb on + strand, within Pf6N2E2_1458 at 1707.307 kb on + strand, within Pf6N2E2_1458 at 1707.307 kb on + strand, within Pf6N2E2_1458 at 1707.308 kb on - strand, within Pf6N2E2_1458 at 1707.337 kb on + strand, within Pf6N2E2_1458 at 1707.337 kb on + strand, within Pf6N2E2_1458 at 1707.338 kb on - strand, within Pf6N2E2_1458 at 1707.338 kb on - strand, within Pf6N2E2_1458 at 1707.338 kb on - strand, within Pf6N2E2_1458 at 1707.340 kb on + strand at 1707.512 kb on - strand, within Pf6N2E2_1458 at 1707.536 kb on - strand, within Pf6N2E2_1458 at 1707.764 kb on - strand at 1707.764 kb on - strand at 1707.845 kb on + strand at 1707.845 kb on + strand at 1707.846 kb on - strand at 1707.854 kb on - strand at 1707.986 kb on + strand, within Pf6N2E2_1459 at 1707.986 kb on + strand, within Pf6N2E2_1459 at 1707.987 kb on - strand, within Pf6N2E2_1459 at 1707.987 kb on - strand, within Pf6N2E2_1459
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.008 mg/ml remove 1,705,043 + Pf6N2E2_1456 0.38 +1.9 1,705,043 + Pf6N2E2_1456 0.38 +0.0 1,705,044 - Pf6N2E2_1456 0.38 +0.5 1,705,044 - Pf6N2E2_1456 0.38 +1.3 1,705,253 + Pf6N2E2_1456 0.52 -0.8 1,705,556 - Pf6N2E2_1456 0.71 +0.8 1,705,559 + Pf6N2E2_1456 0.71 +0.6 1,705,559 + Pf6N2E2_1456 0.71 -1.9 1,705,776 - Pf6N2E2_1456 0.85 +0.2 1,705,776 - Pf6N2E2_1456 0.85 -0.1 1,705,784 + Pf6N2E2_1456 0.86 +0.5 1,705,784 + Pf6N2E2_1456 0.86 -1.3 1,705,784 + Pf6N2E2_1456 0.86 +0.7 1,705,785 - Pf6N2E2_1456 0.86 +0.4 1,705,785 - Pf6N2E2_1456 0.86 +0.3 1,705,982 - +0.2 1,705,982 - +0.2 1,706,351 + Pf6N2E2_1457 0.31 -2.0 1,706,351 + Pf6N2E2_1457 0.31 -0.6 1,706,352 - Pf6N2E2_1457 0.31 +0.1 1,706,466 - Pf6N2E2_1457 0.41 -0.8 1,706,480 + Pf6N2E2_1457 0.42 -0.0 1,706,480 + Pf6N2E2_1457 0.42 -0.7 1,706,608 - Pf6N2E2_1457 0.53 +0.5 1,706,651 + Pf6N2E2_1457 0.57 -0.2 1,706,651 + Pf6N2E2_1457 0.57 -0.8 1,706,654 - Pf6N2E2_1457 0.57 -0.9 1,706,715 + Pf6N2E2_1457 0.63 -0.3 1,706,716 - Pf6N2E2_1457 0.63 -0.6 1,706,723 + Pf6N2E2_1457 0.63 -0.4 1,706,808 + Pf6N2E2_1457 0.71 -0.9 1,706,808 + Pf6N2E2_1457 0.71 +0.7 1,706,808 + Pf6N2E2_1457 0.71 +0.3 1,706,809 - Pf6N2E2_1457 0.71 -0.3 1,706,987 + Pf6N2E2_1457 0.87 +0.8 1,706,987 + Pf6N2E2_1457 0.87 -0.7 1,706,988 - Pf6N2E2_1457 0.87 +0.2 1,706,988 - Pf6N2E2_1457 0.87 -0.2 1,707,060 - -0.5 1,707,144 + +0.1 1,707,144 + -0.3 1,707,305 + Pf6N2E2_1458 0.25 +0.0 1,707,307 + Pf6N2E2_1458 0.26 -1.3 1,707,307 + Pf6N2E2_1458 0.26 -0.1 1,707,307 + Pf6N2E2_1458 0.26 -0.6 1,707,307 + Pf6N2E2_1458 0.26 +0.2 1,707,308 - Pf6N2E2_1458 0.26 -1.0 1,707,337 + Pf6N2E2_1458 0.31 +0.0 1,707,337 + Pf6N2E2_1458 0.31 -0.6 1,707,338 - Pf6N2E2_1458 0.31 -0.1 1,707,338 - Pf6N2E2_1458 0.31 +0.2 1,707,338 - Pf6N2E2_1458 0.31 -0.8 1,707,340 + +2.2 1,707,512 - Pf6N2E2_1458 0.59 -0.3 1,707,536 - Pf6N2E2_1458 0.63 -0.6 1,707,764 - -1.5 1,707,764 - +0.3 1,707,845 + -1.0 1,707,845 + -0.3 1,707,846 - -1.2 1,707,854 - +1.2 1,707,986 + Pf6N2E2_1459 0.13 -0.3 1,707,986 + Pf6N2E2_1459 0.13 +0.3 1,707,987 - Pf6N2E2_1459 0.13 +0.4 1,707,987 - Pf6N2E2_1459 0.13 +0.8
Or see this region's nucleotide sequence