Experiment: Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1249 and Pf6N2E2_1250 are separated by 144 nucleotides Pf6N2E2_1250 and Pf6N2E2_1251 are separated by 553 nucleotides
Pf6N2E2_1249 - Transcriptional regulator, AraC family, at 1,460,242 to 1,461,165
_1249
Pf6N2E2_1250 - Nitric-oxide reductase (EC 1.7.99.7), quinol-dependent, at 1,461,310 to 1,463,583
_1250
Pf6N2E2_1251 - Similar to non-heme chloroperoxidase, sll5080 homolog, at 1,464,137 to 1,464,967
_1251
Position (kb)
1461
1462
1463
1464 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1460.399 kb on + strand, within Pf6N2E2_1249 at 1460.400 kb on - strand, within Pf6N2E2_1249 at 1460.432 kb on + strand, within Pf6N2E2_1249 at 1460.495 kb on + strand, within Pf6N2E2_1249 at 1460.552 kb on + strand, within Pf6N2E2_1249 at 1460.552 kb on + strand, within Pf6N2E2_1249 at 1460.634 kb on + strand, within Pf6N2E2_1249 at 1460.635 kb on - strand, within Pf6N2E2_1249 at 1460.963 kb on + strand, within Pf6N2E2_1249 at 1461.149 kb on + strand at 1461.149 kb on + strand at 1461.149 kb on + strand at 1461.190 kb on + strand at 1461.279 kb on + strand at 1461.312 kb on - strand at 1461.312 kb on - strand at 1461.519 kb on - strand at 1461.519 kb on - strand at 1461.639 kb on - strand, within Pf6N2E2_1250 at 1461.737 kb on + strand, within Pf6N2E2_1250 at 1461.740 kb on + strand, within Pf6N2E2_1250 at 1461.740 kb on + strand, within Pf6N2E2_1250 at 1461.740 kb on + strand, within Pf6N2E2_1250 at 1461.741 kb on - strand, within Pf6N2E2_1250 at 1461.975 kb on - strand, within Pf6N2E2_1250 at 1461.975 kb on - strand, within Pf6N2E2_1250 at 1461.975 kb on - strand, within Pf6N2E2_1250 at 1461.975 kb on - strand, within Pf6N2E2_1250 at 1461.975 kb on - strand, within Pf6N2E2_1250 at 1461.975 kb on - strand, within Pf6N2E2_1250 at 1462.074 kb on + strand, within Pf6N2E2_1250 at 1462.132 kb on + strand, within Pf6N2E2_1250 at 1462.133 kb on - strand, within Pf6N2E2_1250 at 1462.133 kb on - strand, within Pf6N2E2_1250 at 1462.133 kb on - strand, within Pf6N2E2_1250 at 1462.133 kb on - strand, within Pf6N2E2_1250 at 1462.157 kb on + strand, within Pf6N2E2_1250 at 1462.191 kb on - strand, within Pf6N2E2_1250 at 1462.209 kb on - strand, within Pf6N2E2_1250 at 1462.209 kb on - strand, within Pf6N2E2_1250 at 1462.368 kb on - strand, within Pf6N2E2_1250 at 1462.445 kb on + strand, within Pf6N2E2_1250 at 1462.446 kb on - strand, within Pf6N2E2_1250 at 1462.584 kb on + strand, within Pf6N2E2_1250 at 1462.585 kb on - strand, within Pf6N2E2_1250 at 1462.585 kb on - strand, within Pf6N2E2_1250 at 1462.585 kb on - strand, within Pf6N2E2_1250 at 1462.585 kb on - strand, within Pf6N2E2_1250 at 1462.619 kb on + strand, within Pf6N2E2_1250 at 1462.649 kb on + strand, within Pf6N2E2_1250 at 1462.674 kb on - strand, within Pf6N2E2_1250 at 1462.674 kb on - strand, within Pf6N2E2_1250 at 1462.736 kb on + strand, within Pf6N2E2_1250 at 1462.737 kb on - strand, within Pf6N2E2_1250 at 1462.737 kb on - strand, within Pf6N2E2_1250 at 1462.957 kb on - strand at 1462.958 kb on + strand, within Pf6N2E2_1250 at 1462.958 kb on + strand, within Pf6N2E2_1250 at 1462.959 kb on - strand, within Pf6N2E2_1250 at 1462.959 kb on - strand, within Pf6N2E2_1250 at 1462.959 kb on - strand, within Pf6N2E2_1250 at 1462.959 kb on - strand, within Pf6N2E2_1250 at 1462.959 kb on - strand, within Pf6N2E2_1250 at 1462.959 kb on - strand, within Pf6N2E2_1250 at 1463.123 kb on + strand, within Pf6N2E2_1250 at 1463.123 kb on + strand, within Pf6N2E2_1250 at 1463.123 kb on + strand, within Pf6N2E2_1250 at 1463.124 kb on - strand, within Pf6N2E2_1250 at 1463.124 kb on - strand, within Pf6N2E2_1250 at 1463.126 kb on + strand, within Pf6N2E2_1250 at 1463.126 kb on + strand, within Pf6N2E2_1250 at 1463.127 kb on - strand, within Pf6N2E2_1250 at 1463.205 kb on - strand, within Pf6N2E2_1250 at 1463.261 kb on + strand, within Pf6N2E2_1250 at 1463.262 kb on - strand, within Pf6N2E2_1250 at 1463.262 kb on - strand, within Pf6N2E2_1250 at 1463.262 kb on - strand, within Pf6N2E2_1250 at 1463.274 kb on - strand, within Pf6N2E2_1250 at 1463.395 kb on - strand at 1463.425 kb on - strand at 1463.425 kb on - strand at 1463.441 kb on - strand at 1463.496 kb on - strand at 1463.496 kb on - strand at 1463.661 kb on + strand at 1463.797 kb on + strand at 1463.839 kb on - strand at 1463.921 kb on + strand at 1463.922 kb on - strand at 1464.018 kb on + strand at 1464.018 kb on + strand at 1464.018 kb on + strand at 1464.176 kb on + strand at 1464.177 kb on - strand at 1464.263 kb on + strand, within Pf6N2E2_1251 at 1464.263 kb on + strand, within Pf6N2E2_1251 at 1464.399 kb on + strand, within Pf6N2E2_1251 at 1464.399 kb on + strand, within Pf6N2E2_1251 at 1464.399 kb on + strand, within Pf6N2E2_1251 at 1464.400 kb on - strand, within Pf6N2E2_1251 at 1464.400 kb on - strand, within Pf6N2E2_1251 at 1464.400 kb on - strand, within Pf6N2E2_1251 at 1464.403 kb on + strand, within Pf6N2E2_1251 at 1464.403 kb on + strand, within Pf6N2E2_1251 at 1464.403 kb on + strand, within Pf6N2E2_1251 at 1464.403 kb on + strand, within Pf6N2E2_1251 at 1464.403 kb on + strand, within Pf6N2E2_1251 at 1464.404 kb on - strand, within Pf6N2E2_1251 at 1464.404 kb on - strand, within Pf6N2E2_1251
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.008 mg/ml remove 1,460,399 + Pf6N2E2_1249 0.17 -0.2 1,460,400 - Pf6N2E2_1249 0.17 +0.3 1,460,432 + Pf6N2E2_1249 0.21 +0.0 1,460,495 + Pf6N2E2_1249 0.27 +1.5 1,460,552 + Pf6N2E2_1249 0.34 -0.3 1,460,552 + Pf6N2E2_1249 0.34 +0.1 1,460,634 + Pf6N2E2_1249 0.42 -0.5 1,460,635 - Pf6N2E2_1249 0.43 -0.2 1,460,963 + Pf6N2E2_1249 0.78 +0.5 1,461,149 + -0.5 1,461,149 + +0.3 1,461,149 + -0.3 1,461,190 + -0.4 1,461,279 + +0.5 1,461,312 - +0.1 1,461,312 - +0.7 1,461,519 - +0.8 1,461,519 - +0.2 1,461,639 - Pf6N2E2_1250 0.14 -0.4 1,461,737 + Pf6N2E2_1250 0.19 +0.4 1,461,740 + Pf6N2E2_1250 0.19 -2.6 1,461,740 + Pf6N2E2_1250 0.19 -1.1 1,461,740 + Pf6N2E2_1250 0.19 -0.5 1,461,741 - Pf6N2E2_1250 0.19 -0.5 1,461,975 - Pf6N2E2_1250 0.29 +0.3 1,461,975 - Pf6N2E2_1250 0.29 +0.4 1,461,975 - Pf6N2E2_1250 0.29 -0.1 1,461,975 - Pf6N2E2_1250 0.29 +0.1 1,461,975 - Pf6N2E2_1250 0.29 -0.7 1,461,975 - Pf6N2E2_1250 0.29 -0.6 1,462,074 + Pf6N2E2_1250 0.34 +2.0 1,462,132 + Pf6N2E2_1250 0.36 +0.2 1,462,133 - Pf6N2E2_1250 0.36 -0.1 1,462,133 - Pf6N2E2_1250 0.36 -1.0 1,462,133 - Pf6N2E2_1250 0.36 +0.0 1,462,133 - Pf6N2E2_1250 0.36 -0.1 1,462,157 + Pf6N2E2_1250 0.37 +0.5 1,462,191 - Pf6N2E2_1250 0.39 -0.9 1,462,209 - Pf6N2E2_1250 0.40 +1.1 1,462,209 - Pf6N2E2_1250 0.40 +0.4 1,462,368 - Pf6N2E2_1250 0.47 +0.1 1,462,445 + Pf6N2E2_1250 0.50 -0.2 1,462,446 - Pf6N2E2_1250 0.50 +1.6 1,462,584 + Pf6N2E2_1250 0.56 +0.3 1,462,585 - Pf6N2E2_1250 0.56 +1.0 1,462,585 - Pf6N2E2_1250 0.56 +0.1 1,462,585 - Pf6N2E2_1250 0.56 +0.5 1,462,585 - Pf6N2E2_1250 0.56 -0.7 1,462,619 + Pf6N2E2_1250 0.58 +0.1 1,462,649 + Pf6N2E2_1250 0.59 +0.8 1,462,674 - Pf6N2E2_1250 0.60 -0.1 1,462,674 - Pf6N2E2_1250 0.60 -0.3 1,462,736 + Pf6N2E2_1250 0.63 -0.9 1,462,737 - Pf6N2E2_1250 0.63 +0.7 1,462,737 - Pf6N2E2_1250 0.63 -0.6 1,462,957 - +2.1 1,462,958 + Pf6N2E2_1250 0.72 -0.4 1,462,958 + Pf6N2E2_1250 0.72 +0.1 1,462,959 - Pf6N2E2_1250 0.73 +0.8 1,462,959 - Pf6N2E2_1250 0.73 +1.0 1,462,959 - Pf6N2E2_1250 0.73 +0.0 1,462,959 - Pf6N2E2_1250 0.73 -0.5 1,462,959 - Pf6N2E2_1250 0.73 +0.6 1,462,959 - Pf6N2E2_1250 0.73 -1.3 1,463,123 + Pf6N2E2_1250 0.80 -1.0 1,463,123 + Pf6N2E2_1250 0.80 -0.4 1,463,123 + Pf6N2E2_1250 0.80 -0.9 1,463,124 - Pf6N2E2_1250 0.80 +1.3 1,463,124 - Pf6N2E2_1250 0.80 -0.2 1,463,126 + Pf6N2E2_1250 0.80 -1.0 1,463,126 + Pf6N2E2_1250 0.80 -0.0 1,463,127 - Pf6N2E2_1250 0.80 +0.4 1,463,205 - Pf6N2E2_1250 0.83 +0.4 1,463,261 + Pf6N2E2_1250 0.86 -0.7 1,463,262 - Pf6N2E2_1250 0.86 +0.1 1,463,262 - Pf6N2E2_1250 0.86 -1.3 1,463,262 - Pf6N2E2_1250 0.86 +0.8 1,463,274 - Pf6N2E2_1250 0.86 -0.4 1,463,395 - -0.5 1,463,425 - +0.3 1,463,425 - +0.8 1,463,441 - +0.5 1,463,496 - -1.9 1,463,496 - -0.0 1,463,661 + +0.5 1,463,797 + -0.3 1,463,839 - +0.8 1,463,921 + -0.1 1,463,922 - +0.7 1,464,018 + -1.1 1,464,018 + +1.2 1,464,018 + -0.6 1,464,176 + +0.4 1,464,177 - -0.8 1,464,263 + Pf6N2E2_1251 0.15 +0.4 1,464,263 + Pf6N2E2_1251 0.15 +0.8 1,464,399 + Pf6N2E2_1251 0.32 +0.2 1,464,399 + Pf6N2E2_1251 0.32 +1.2 1,464,399 + Pf6N2E2_1251 0.32 -0.5 1,464,400 - Pf6N2E2_1251 0.32 +0.2 1,464,400 - Pf6N2E2_1251 0.32 +0.8 1,464,400 - Pf6N2E2_1251 0.32 -0.0 1,464,403 + Pf6N2E2_1251 0.32 +0.7 1,464,403 + Pf6N2E2_1251 0.32 -2.4 1,464,403 + Pf6N2E2_1251 0.32 +1.2 1,464,403 + Pf6N2E2_1251 0.32 +0.2 1,464,403 + Pf6N2E2_1251 0.32 -0.1 1,464,404 - Pf6N2E2_1251 0.32 +2.2 1,464,404 - Pf6N2E2_1251 0.32 +1.0
Or see this region's nucleotide sequence