Experiment: Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1236 and Pf6N2E2_1237 are separated by 132 nucleotides Pf6N2E2_1237 and Pf6N2E2_1238 are separated by 283 nucleotides Pf6N2E2_1238 and Pf6N2E2_1239 are separated by 270 nucleotides
Pf6N2E2_1236 - Transcriptional regulator, LysR family, at 1,449,005 to 1,449,901
_1236
Pf6N2E2_1237 - Putative transport protein, at 1,450,034 to 1,451,497
_1237
Pf6N2E2_1238 - hypothetical protein, at 1,451,781 to 1,451,969
_1238
Pf6N2E2_1239 - Putative TEGT family carrier/transport protein, at 1,452,240 to 1,452,911
_1239
Position (kb)
1450
1451
1452 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1449.163 kb on - strand, within Pf6N2E2_1236 at 1449.425 kb on + strand, within Pf6N2E2_1236 at 1449.425 kb on + strand, within Pf6N2E2_1236 at 1449.426 kb on - strand, within Pf6N2E2_1236 at 1449.941 kb on + strand at 1449.942 kb on - strand at 1450.236 kb on - strand, within Pf6N2E2_1237 at 1450.236 kb on - strand, within Pf6N2E2_1237 at 1450.347 kb on + strand, within Pf6N2E2_1237 at 1450.488 kb on + strand, within Pf6N2E2_1237 at 1450.489 kb on - strand, within Pf6N2E2_1237 at 1450.622 kb on + strand, within Pf6N2E2_1237 at 1450.715 kb on + strand, within Pf6N2E2_1237 at 1450.715 kb on + strand, within Pf6N2E2_1237 at 1450.715 kb on + strand, within Pf6N2E2_1237 at 1450.715 kb on + strand, within Pf6N2E2_1237 at 1450.715 kb on + strand, within Pf6N2E2_1237 at 1450.715 kb on + strand, within Pf6N2E2_1237 at 1450.716 kb on - strand, within Pf6N2E2_1237 at 1450.716 kb on - strand, within Pf6N2E2_1237 at 1450.716 kb on - strand, within Pf6N2E2_1237 at 1450.716 kb on - strand, within Pf6N2E2_1237 at 1450.716 kb on - strand, within Pf6N2E2_1237 at 1450.716 kb on - strand, within Pf6N2E2_1237 at 1450.800 kb on + strand, within Pf6N2E2_1237 at 1450.873 kb on - strand, within Pf6N2E2_1237 at 1450.940 kb on + strand, within Pf6N2E2_1237 at 1450.940 kb on + strand, within Pf6N2E2_1237 at 1450.941 kb on - strand, within Pf6N2E2_1237 at 1451.026 kb on - strand, within Pf6N2E2_1237 at 1451.129 kb on - strand, within Pf6N2E2_1237 at 1451.147 kb on + strand, within Pf6N2E2_1237 at 1451.180 kb on + strand, within Pf6N2E2_1237 at 1451.226 kb on + strand, within Pf6N2E2_1237 at 1451.227 kb on - strand, within Pf6N2E2_1237 at 1451.227 kb on - strand, within Pf6N2E2_1237 at 1451.601 kb on + strand at 1451.608 kb on - strand at 1451.620 kb on + strand at 1451.620 kb on + strand at 1451.717 kb on + strand at 1451.717 kb on + strand at 1451.717 kb on + strand at 1451.717 kb on + strand at 1451.717 kb on + strand at 1451.718 kb on - strand at 1451.718 kb on - strand at 1451.740 kb on + strand at 1451.899 kb on + strand, within Pf6N2E2_1238 at 1451.935 kb on + strand, within Pf6N2E2_1238 at 1451.935 kb on + strand, within Pf6N2E2_1238 at 1451.967 kb on + strand at 1451.967 kb on + strand at 1451.967 kb on + strand at 1451.967 kb on + strand at 1451.968 kb on - strand at 1451.968 kb on - strand at 1451.988 kb on + strand at 1451.989 kb on - strand at 1452.069 kb on - strand at 1452.084 kb on + strand at 1452.084 kb on + strand at 1452.084 kb on + strand at 1452.084 kb on + strand at 1452.084 kb on + strand at 1452.084 kb on + strand at 1452.084 kb on + strand at 1452.084 kb on + strand at 1452.084 kb on + strand at 1452.085 kb on - strand at 1452.085 kb on - strand at 1452.085 kb on - strand at 1452.085 kb on - strand at 1452.085 kb on - strand at 1452.085 kb on - strand at 1452.085 kb on - strand at 1452.085 kb on - strand at 1452.085 kb on - strand at 1452.085 kb on - strand at 1452.086 kb on + strand at 1452.130 kb on + strand at 1452.130 kb on + strand at 1452.130 kb on + strand at 1452.131 kb on - strand at 1452.189 kb on - strand at 1452.296 kb on - strand at 1452.296 kb on - strand at 1452.303 kb on + strand at 1452.303 kb on + strand at 1452.304 kb on - strand at 1452.304 kb on - strand at 1452.307 kb on + strand at 1452.308 kb on - strand, within Pf6N2E2_1239 at 1452.308 kb on - strand, within Pf6N2E2_1239 at 1452.308 kb on - strand, within Pf6N2E2_1239 at 1452.308 kb on - strand, within Pf6N2E2_1239 at 1452.308 kb on - strand, within Pf6N2E2_1239 at 1452.308 kb on - strand, within Pf6N2E2_1239 at 1452.308 kb on - strand, within Pf6N2E2_1239 at 1452.308 kb on - strand, within Pf6N2E2_1239 at 1452.308 kb on - strand, within Pf6N2E2_1239 at 1452.332 kb on - strand, within Pf6N2E2_1239 at 1452.332 kb on - strand, within Pf6N2E2_1239 at 1452.332 kb on - strand, within Pf6N2E2_1239 at 1452.332 kb on - strand, within Pf6N2E2_1239 at 1452.332 kb on - strand, within Pf6N2E2_1239 at 1452.332 kb on - strand, within Pf6N2E2_1239 at 1452.332 kb on - strand, within Pf6N2E2_1239 at 1452.332 kb on - strand, within Pf6N2E2_1239 at 1452.335 kb on + strand, within Pf6N2E2_1239
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.008 mg/ml remove 1,449,163 - Pf6N2E2_1236 0.18 +0.3 1,449,425 + Pf6N2E2_1236 0.47 +0.2 1,449,425 + Pf6N2E2_1236 0.47 -0.4 1,449,426 - Pf6N2E2_1236 0.47 -0.2 1,449,941 + -0.2 1,449,942 - -0.3 1,450,236 - Pf6N2E2_1237 0.14 +1.9 1,450,236 - Pf6N2E2_1237 0.14 -0.2 1,450,347 + Pf6N2E2_1237 0.21 +0.5 1,450,488 + Pf6N2E2_1237 0.31 -0.6 1,450,489 - Pf6N2E2_1237 0.31 +0.9 1,450,622 + Pf6N2E2_1237 0.40 +1.0 1,450,715 + Pf6N2E2_1237 0.47 +0.1 1,450,715 + Pf6N2E2_1237 0.47 -2.1 1,450,715 + Pf6N2E2_1237 0.47 +0.8 1,450,715 + Pf6N2E2_1237 0.47 -1.1 1,450,715 + Pf6N2E2_1237 0.47 +0.7 1,450,715 + Pf6N2E2_1237 0.47 +0.1 1,450,716 - Pf6N2E2_1237 0.47 +1.0 1,450,716 - Pf6N2E2_1237 0.47 +1.0 1,450,716 - Pf6N2E2_1237 0.47 +0.3 1,450,716 - Pf6N2E2_1237 0.47 +1.2 1,450,716 - Pf6N2E2_1237 0.47 +0.9 1,450,716 - Pf6N2E2_1237 0.47 -1.6 1,450,800 + Pf6N2E2_1237 0.52 +0.8 1,450,873 - Pf6N2E2_1237 0.57 +0.1 1,450,940 + Pf6N2E2_1237 0.62 +0.8 1,450,940 + Pf6N2E2_1237 0.62 +0.3 1,450,941 - Pf6N2E2_1237 0.62 -1.3 1,451,026 - Pf6N2E2_1237 0.68 +2.4 1,451,129 - Pf6N2E2_1237 0.75 -0.6 1,451,147 + Pf6N2E2_1237 0.76 +0.5 1,451,180 + Pf6N2E2_1237 0.78 +0.5 1,451,226 + Pf6N2E2_1237 0.81 -0.7 1,451,227 - Pf6N2E2_1237 0.81 +0.6 1,451,227 - Pf6N2E2_1237 0.81 -1.2 1,451,601 + -2.8 1,451,608 - -1.5 1,451,620 + +0.3 1,451,620 + -0.1 1,451,717 + -0.5 1,451,717 + -0.3 1,451,717 + -0.7 1,451,717 + -1.7 1,451,717 + +0.1 1,451,718 - -1.3 1,451,718 - -0.0 1,451,740 + -2.7 1,451,899 + Pf6N2E2_1238 0.62 +0.6 1,451,935 + Pf6N2E2_1238 0.81 -0.1 1,451,935 + Pf6N2E2_1238 0.81 -0.6 1,451,967 + +0.3 1,451,967 + -1.0 1,451,967 + +0.2 1,451,967 + +0.2 1,451,968 - +0.1 1,451,968 - +0.1 1,451,988 + -1.4 1,451,989 - +1.1 1,452,069 - +0.2 1,452,084 + -0.1 1,452,084 + -0.5 1,452,084 + -1.5 1,452,084 + +0.2 1,452,084 + -0.1 1,452,084 + +0.9 1,452,084 + -0.9 1,452,084 + +0.6 1,452,084 + -0.3 1,452,085 - -1.0 1,452,085 - +0.5 1,452,085 - +0.5 1,452,085 - +0.3 1,452,085 - +0.2 1,452,085 - -0.1 1,452,085 - +0.8 1,452,085 - -1.1 1,452,085 - -0.1 1,452,085 - -1.6 1,452,086 + +0.1 1,452,130 + -0.2 1,452,130 + -0.8 1,452,130 + -0.2 1,452,131 - +0.7 1,452,189 - +0.6 1,452,296 - -0.5 1,452,296 - +0.1 1,452,303 + -0.6 1,452,303 + -1.0 1,452,304 - -1.1 1,452,304 - +0.4 1,452,307 + +1.5 1,452,308 - Pf6N2E2_1239 0.10 -0.9 1,452,308 - Pf6N2E2_1239 0.10 +0.1 1,452,308 - Pf6N2E2_1239 0.10 -2.7 1,452,308 - Pf6N2E2_1239 0.10 +0.3 1,452,308 - Pf6N2E2_1239 0.10 +0.2 1,452,308 - Pf6N2E2_1239 0.10 -0.6 1,452,308 - Pf6N2E2_1239 0.10 -0.1 1,452,308 - Pf6N2E2_1239 0.10 -2.2 1,452,308 - Pf6N2E2_1239 0.10 -0.8 1,452,332 - Pf6N2E2_1239 0.14 -0.4 1,452,332 - Pf6N2E2_1239 0.14 -0.5 1,452,332 - Pf6N2E2_1239 0.14 -0.3 1,452,332 - Pf6N2E2_1239 0.14 -0.5 1,452,332 - Pf6N2E2_1239 0.14 -0.1 1,452,332 - Pf6N2E2_1239 0.14 +1.1 1,452,332 - Pf6N2E2_1239 0.14 -0.2 1,452,332 - Pf6N2E2_1239 0.14 -0.8 1,452,335 + Pf6N2E2_1239 0.14 -1.9
Or see this region's nucleotide sequence