Experiment: Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1080 and Pf6N2E2_1081 are separated by 85 nucleotides Pf6N2E2_1081 and Pf6N2E2_1082 overlap by 1 nucleotides Pf6N2E2_1082 and Pf6N2E2_1083 are separated by 230 nucleotides Pf6N2E2_1083 and Pf6N2E2_1084 are separated by 150 nucleotides
Pf6N2E2_1080 - hydrolase, alpha/beta fold family, at 1,264,415 to 1,265,446
_1080
Pf6N2E2_1081 - probable membrane protein YPO3302, at 1,265,532 to 1,265,966
_1081
Pf6N2E2_1082 - CopG protein, at 1,265,966 to 1,266,412
_1082
Pf6N2E2_1083 - hypothetical protein, at 1,266,643 to 1,266,780
_1083
Pf6N2E2_1084 - hypothetical protein, at 1,266,931 to 1,267,119
_1084
Position (kb)
1265
1266 Strain fitness (log2 ratio)
-2
-1
0
1 at 1264.554 kb on - strand, within Pf6N2E2_1080 at 1264.560 kb on + strand, within Pf6N2E2_1080 at 1264.560 kb on + strand, within Pf6N2E2_1080 at 1264.674 kb on + strand, within Pf6N2E2_1080 at 1264.675 kb on - strand, within Pf6N2E2_1080 at 1264.701 kb on + strand, within Pf6N2E2_1080 at 1264.701 kb on + strand, within Pf6N2E2_1080 at 1264.701 kb on + strand, within Pf6N2E2_1080 at 1264.702 kb on - strand, within Pf6N2E2_1080 at 1264.893 kb on - strand at 1265.028 kb on - strand, within Pf6N2E2_1080 at 1265.089 kb on - strand, within Pf6N2E2_1080 at 1265.102 kb on - strand, within Pf6N2E2_1080 at 1265.263 kb on - strand, within Pf6N2E2_1080 at 1265.316 kb on + strand, within Pf6N2E2_1080 at 1265.317 kb on - strand, within Pf6N2E2_1080 at 1265.322 kb on + strand, within Pf6N2E2_1080 at 1265.350 kb on - strand at 1265.492 kb on - strand at 1265.710 kb on + strand, within Pf6N2E2_1081 at 1265.953 kb on + strand at 1265.953 kb on + strand at 1265.953 kb on + strand at 1265.953 kb on + strand at 1265.953 kb on + strand at 1265.954 kb on - strand at 1265.954 kb on - strand at 1266.043 kb on + strand, within Pf6N2E2_1082 at 1266.043 kb on + strand, within Pf6N2E2_1082 at 1266.044 kb on - strand, within Pf6N2E2_1082 at 1266.495 kb on - strand at 1266.587 kb on - strand at 1266.602 kb on + strand at 1266.602 kb on + strand at 1266.602 kb on + strand at 1266.644 kb on + strand at 1266.760 kb on + strand, within Pf6N2E2_1083 at 1266.760 kb on + strand, within Pf6N2E2_1083 at 1266.761 kb on - strand, within Pf6N2E2_1083 at 1266.780 kb on + strand at 1266.780 kb on + strand at 1266.780 kb on + strand at 1266.780 kb on + strand at 1266.780 kb on + strand at 1266.780 kb on + strand at 1266.781 kb on - strand at 1266.781 kb on - strand at 1266.781 kb on - strand at 1266.781 kb on - strand at 1266.781 kb on - strand at 1266.784 kb on + strand at 1266.784 kb on + strand at 1266.784 kb on + strand at 1266.784 kb on + strand at 1266.784 kb on + strand at 1266.785 kb on - strand at 1266.785 kb on - strand at 1266.785 kb on - strand at 1266.785 kb on - strand at 1266.785 kb on - strand at 1266.785 kb on - strand at 1266.785 kb on - strand at 1266.785 kb on - strand at 1266.785 kb on - strand at 1266.819 kb on + strand at 1266.820 kb on - strand at 1266.835 kb on + strand at 1266.874 kb on - strand at 1266.912 kb on + strand at 1266.913 kb on - strand at 1266.929 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.008 mg/ml remove 1,264,554 - Pf6N2E2_1080 0.13 +1.0 1,264,560 + Pf6N2E2_1080 0.14 -0.7 1,264,560 + Pf6N2E2_1080 0.14 +0.3 1,264,674 + Pf6N2E2_1080 0.25 +0.8 1,264,675 - Pf6N2E2_1080 0.25 -1.3 1,264,701 + Pf6N2E2_1080 0.28 -2.0 1,264,701 + Pf6N2E2_1080 0.28 -1.0 1,264,701 + Pf6N2E2_1080 0.28 +0.5 1,264,702 - Pf6N2E2_1080 0.28 -0.6 1,264,893 - -0.3 1,265,028 - Pf6N2E2_1080 0.59 +0.1 1,265,089 - Pf6N2E2_1080 0.65 -0.3 1,265,102 - Pf6N2E2_1080 0.67 -0.4 1,265,263 - Pf6N2E2_1080 0.82 -0.5 1,265,316 + Pf6N2E2_1080 0.87 +0.2 1,265,317 - Pf6N2E2_1080 0.87 +0.0 1,265,322 + Pf6N2E2_1080 0.88 -1.9 1,265,350 - +0.5 1,265,492 - +0.2 1,265,710 + Pf6N2E2_1081 0.41 -1.0 1,265,953 + -1.6 1,265,953 + +0.9 1,265,953 + -0.3 1,265,953 + +0.7 1,265,953 + -0.1 1,265,954 - +0.7 1,265,954 - +0.6 1,266,043 + Pf6N2E2_1082 0.17 +0.3 1,266,043 + Pf6N2E2_1082 0.17 -1.6 1,266,044 - Pf6N2E2_1082 0.17 +0.7 1,266,495 - -0.7 1,266,587 - -1.1 1,266,602 + -0.7 1,266,602 + -1.0 1,266,602 + -1.9 1,266,644 + +1.2 1,266,760 + Pf6N2E2_1083 0.85 +0.4 1,266,760 + Pf6N2E2_1083 0.85 +0.6 1,266,761 - Pf6N2E2_1083 0.86 -0.2 1,266,780 + +0.4 1,266,780 + +0.3 1,266,780 + +0.4 1,266,780 + -0.5 1,266,780 + +0.2 1,266,780 + +0.0 1,266,781 - -1.2 1,266,781 - -0.2 1,266,781 - +0.2 1,266,781 - +0.6 1,266,781 - +0.5 1,266,784 + -1.2 1,266,784 + -0.0 1,266,784 + +0.8 1,266,784 + +1.3 1,266,784 + -0.3 1,266,785 - +0.7 1,266,785 - -0.1 1,266,785 - -0.2 1,266,785 - -0.1 1,266,785 - +0.3 1,266,785 - +0.3 1,266,785 - -1.3 1,266,785 - +0.1 1,266,785 - -0.6 1,266,819 + +0.2 1,266,820 - +1.3 1,266,835 + -0.4 1,266,874 - -1.9 1,266,912 + +0.7 1,266,913 - +0.2 1,266,929 + -0.9
Or see this region's nucleotide sequence