Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08200 and MMP_RS08205 are separated by 79 nucleotides MMP_RS08205 and MMP_RS08210 are separated by 19 nucleotides MMP_RS08210 and MMP_RS08215 are separated by 82 nucleotides MMP_RS08215 and MMP_RS08220 are separated by 15 nucleotides
MMP_RS08200: MMP_RS08200 - archaeosine synthase subunit alpha, at 1,543,851 to 1,545,467
_RS08200
MMP_RS08205: MMP_RS08205 - stage II sporulation protein M, at 1,545,547 to 1,546,137
_RS08205
MMP_RS08210: MMP_RS08210 - phosphatidylglycerophosphatase A, at 1,546,157 to 1,546,645
_RS08210
MMP_RS08215: MMP_RS08215 - DUF483 domain-containing protein, at 1,546,728 to 1,547,282
_RS08215
MMP_RS08220: MMP_RS08220 - TrkA family potassium uptake protein, at 1,547,298 to 1,547,963
_RS08220
Position (kb)
1546
1547 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1545.187 kb on + strand, within MMP_RS08200 at 1545.448 kb on - strand at 1545.533 kb on + strand at 1545.541 kb on + strand at 1545.541 kb on + strand at 1545.545 kb on - strand at 1545.546 kb on + strand at 1545.546 kb on + strand at 1545.549 kb on + strand at 1545.578 kb on + strand at 1545.584 kb on + strand at 1545.654 kb on + strand, within MMP_RS08205 at 1545.663 kb on + strand, within MMP_RS08205 at 1545.809 kb on - strand, within MMP_RS08205 at 1545.815 kb on + strand, within MMP_RS08205 at 1545.842 kb on + strand, within MMP_RS08205 at 1545.864 kb on - strand, within MMP_RS08205 at 1545.865 kb on + strand, within MMP_RS08205 at 1545.865 kb on + strand, within MMP_RS08205 at 1545.866 kb on - strand, within MMP_RS08205 at 1545.867 kb on + strand, within MMP_RS08205 at 1545.867 kb on + strand, within MMP_RS08205 at 1545.867 kb on + strand, within MMP_RS08205 at 1545.867 kb on + strand, within MMP_RS08205 at 1545.867 kb on + strand, within MMP_RS08205 at 1545.870 kb on - strand, within MMP_RS08205 at 1545.870 kb on - strand, within MMP_RS08205 at 1546.025 kb on + strand, within MMP_RS08205 at 1546.084 kb on + strand at 1546.258 kb on - strand, within MMP_RS08210 at 1546.278 kb on + strand, within MMP_RS08210 at 1546.508 kb on + strand, within MMP_RS08210 at 1546.590 kb on - strand, within MMP_RS08210 at 1546.651 kb on - strand at 1546.685 kb on + strand at 1546.693 kb on - strand at 1546.694 kb on + strand at 1546.695 kb on - strand at 1546.696 kb on + strand at 1546.696 kb on + strand at 1546.746 kb on - strand at 1546.840 kb on + strand, within MMP_RS08215 at 1547.033 kb on + strand, within MMP_RS08215 at 1547.033 kb on + strand, within MMP_RS08215 at 1547.064 kb on + strand, within MMP_RS08215 at 1547.374 kb on - strand at 1547.500 kb on - strand at 1547.501 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE remove 1,545,187 + MMP_RS08200 0.83 -3.8 1,545,448 - -4.3 1,545,533 + -3.3 1,545,541 + -3.4 1,545,541 + -2.9 1,545,545 - -1.4 1,545,546 + -2.1 1,545,546 + -3.8 1,545,549 + -0.1 1,545,578 + -0.1 1,545,584 + +0.7 1,545,654 + MMP_RS08205 0.18 +0.3 1,545,663 + MMP_RS08205 0.20 +0.3 1,545,809 - MMP_RS08205 0.44 -0.0 1,545,815 + MMP_RS08205 0.45 +0.1 1,545,842 + MMP_RS08205 0.50 +0.3 1,545,864 - MMP_RS08205 0.54 +0.4 1,545,865 + MMP_RS08205 0.54 +0.3 1,545,865 + MMP_RS08205 0.54 +0.6 1,545,866 - MMP_RS08205 0.54 +0.2 1,545,867 + MMP_RS08205 0.54 +0.7 1,545,867 + MMP_RS08205 0.54 +0.0 1,545,867 + MMP_RS08205 0.54 -0.5 1,545,867 + MMP_RS08205 0.54 +0.3 1,545,867 + MMP_RS08205 0.54 +0.7 1,545,870 - MMP_RS08205 0.55 +1.9 1,545,870 - MMP_RS08205 0.55 +0.6 1,546,025 + MMP_RS08205 0.81 +0.4 1,546,084 + +0.2 1,546,258 - MMP_RS08210 0.21 -4.3 1,546,278 + MMP_RS08210 0.25 -3.5 1,546,508 + MMP_RS08210 0.72 -1.0 1,546,590 - MMP_RS08210 0.89 -3.2 1,546,651 - +0.3 1,546,685 + +1.0 1,546,693 - +0.3 1,546,694 + +0.8 1,546,695 - -1.4 1,546,696 + +0.4 1,546,696 + +1.0 1,546,746 - -1.4 1,546,840 + MMP_RS08215 0.20 +0.7 1,547,033 + MMP_RS08215 0.55 -0.5 1,547,033 + MMP_RS08215 0.55 +0.3 1,547,064 + MMP_RS08215 0.61 +0.3 1,547,374 - -0.8 1,547,500 - -1.8 1,547,501 + -3.0
Or see this region's nucleotide sequence