Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08185 and MMP_RS08190 are separated by 27 nucleotides MMP_RS08190 and MMP_RS08195 are separated by 11 nucleotides MMP_RS08195 and MMP_RS08200 are separated by 86 nucleotides
MMP_RS08185: MMP_RS08185 - tryptophan--tRNA ligase, at 1,541,201 to 1,542,277
_RS08185
MMP_RS08190: MMP_RS08190 - DUF1743 domain-containing protein, at 1,542,305 to 1,543,210
_RS08190
MMP_RS08195: MMP_RS08195 - hypothetical protein, at 1,543,222 to 1,543,764
_RS08195
MMP_RS08200: MMP_RS08200 - archaeosine synthase subunit alpha, at 1,543,851 to 1,545,467
_RS08200
Position (kb)
1543
1544 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1542.451 kb on - strand at 1542.470 kb on + strand at 1542.591 kb on - strand at 1542.649 kb on - strand at 1542.963 kb on - strand at 1543.179 kb on + strand at 1543.196 kb on - strand at 1543.201 kb on + strand at 1543.223 kb on - strand at 1543.223 kb on - strand at 1543.224 kb on + strand at 1543.224 kb on + strand at 1543.224 kb on + strand at 1543.244 kb on - strand at 1543.244 kb on - strand at 1543.245 kb on + strand at 1543.277 kb on + strand, within MMP_RS08195 at 1543.331 kb on - strand, within MMP_RS08195 at 1543.332 kb on + strand, within MMP_RS08195 at 1543.403 kb on - strand, within MMP_RS08195 at 1543.403 kb on - strand, within MMP_RS08195 at 1543.404 kb on + strand, within MMP_RS08195 at 1543.404 kb on + strand, within MMP_RS08195 at 1543.404 kb on + strand, within MMP_RS08195 at 1543.404 kb on + strand, within MMP_RS08195 at 1543.404 kb on + strand, within MMP_RS08195 at 1543.404 kb on + strand, within MMP_RS08195 at 1543.404 kb on + strand, within MMP_RS08195 at 1543.405 kb on - strand, within MMP_RS08195 at 1543.405 kb on - strand, within MMP_RS08195 at 1543.406 kb on + strand, within MMP_RS08195 at 1543.407 kb on - strand, within MMP_RS08195 at 1543.407 kb on - strand, within MMP_RS08195 at 1543.418 kb on + strand, within MMP_RS08195 at 1543.425 kb on - strand, within MMP_RS08195 at 1543.515 kb on - strand, within MMP_RS08195 at 1543.550 kb on - strand, within MMP_RS08195 at 1543.551 kb on + strand, within MMP_RS08195 at 1543.595 kb on + strand, within MMP_RS08195 at 1543.754 kb on - strand at 1543.947 kb on + strand at 1544.036 kb on - strand, within MMP_RS08200 at 1544.178 kb on - strand, within MMP_RS08200 at 1544.286 kb on - strand, within MMP_RS08200 at 1544.287 kb on + strand, within MMP_RS08200 at 1544.317 kb on + strand, within MMP_RS08200 at 1544.390 kb on + strand, within MMP_RS08200 at 1544.409 kb on - strand, within MMP_RS08200 at 1544.409 kb on - strand, within MMP_RS08200 at 1544.413 kb on + strand, within MMP_RS08200 at 1544.418 kb on - strand, within MMP_RS08200 at 1544.465 kb on + strand, within MMP_RS08200 at 1544.475 kb on + strand, within MMP_RS08200 at 1544.586 kb on - strand, within MMP_RS08200 at 1544.606 kb on - strand, within MMP_RS08200 at 1544.606 kb on - strand, within MMP_RS08200 at 1544.670 kb on - strand, within MMP_RS08200 at 1544.676 kb on - strand, within MMP_RS08200 at 1544.677 kb on + strand, within MMP_RS08200 at 1544.682 kb on - strand, within MMP_RS08200 at 1544.682 kb on - strand, within MMP_RS08200 at 1544.683 kb on + strand, within MMP_RS08200 at 1544.690 kb on + strand, within MMP_RS08200 at 1544.690 kb on - strand, within MMP_RS08200 at 1544.690 kb on - strand, within MMP_RS08200 at 1544.690 kb on - strand, within MMP_RS08200 at 1544.691 kb on + strand, within MMP_RS08200 at 1544.691 kb on + strand, within MMP_RS08200 at 1544.693 kb on + strand, within MMP_RS08200 at 1544.693 kb on + strand, within MMP_RS08200 at 1544.702 kb on + strand, within MMP_RS08200 at 1544.721 kb on + strand, within MMP_RS08200 at 1544.723 kb on + strand, within MMP_RS08200 at 1544.723 kb on + strand, within MMP_RS08200 at 1544.761 kb on + strand, within MMP_RS08200
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE remove 1,542,451 - -2.0 1,542,470 + -2.9 1,542,591 - -2.5 1,542,649 - -3.0 1,542,963 - -2.8 1,543,179 + -1.6 1,543,196 - -2.4 1,543,201 + -2.8 1,543,223 - -1.5 1,543,223 - -3.0 1,543,224 + -4.0 1,543,224 + -3.6 1,543,224 + -3.9 1,543,244 - -1.2 1,543,244 - -0.0 1,543,245 + -3.7 1,543,277 + MMP_RS08195 0.10 -3.1 1,543,331 - MMP_RS08195 0.20 -2.3 1,543,332 + MMP_RS08195 0.20 -2.3 1,543,403 - MMP_RS08195 0.33 -1.8 1,543,403 - MMP_RS08195 0.33 -2.0 1,543,404 + MMP_RS08195 0.34 -4.1 1,543,404 + MMP_RS08195 0.34 +0.0 1,543,404 + MMP_RS08195 0.34 -3.4 1,543,404 + MMP_RS08195 0.34 -3.1 1,543,404 + MMP_RS08195 0.34 -2.9 1,543,404 + MMP_RS08195 0.34 -2.6 1,543,404 + MMP_RS08195 0.34 -1.9 1,543,405 - MMP_RS08195 0.34 -4.2 1,543,405 - MMP_RS08195 0.34 -1.4 1,543,406 + MMP_RS08195 0.34 -3.8 1,543,407 - MMP_RS08195 0.34 -1.0 1,543,407 - MMP_RS08195 0.34 -3.5 1,543,418 + MMP_RS08195 0.36 -2.7 1,543,425 - MMP_RS08195 0.37 +0.1 1,543,515 - MMP_RS08195 0.54 -1.8 1,543,550 - MMP_RS08195 0.60 -1.2 1,543,551 + MMP_RS08195 0.61 -0.9 1,543,595 + MMP_RS08195 0.69 -2.5 1,543,754 - -2.3 1,543,947 + -2.9 1,544,036 - MMP_RS08200 0.11 -4.1 1,544,178 - MMP_RS08200 0.20 -4.7 1,544,286 - MMP_RS08200 0.27 -4.8 1,544,287 + MMP_RS08200 0.27 -4.1 1,544,317 + MMP_RS08200 0.29 -3.9 1,544,390 + MMP_RS08200 0.33 -5.6 1,544,409 - MMP_RS08200 0.35 -4.6 1,544,409 - MMP_RS08200 0.35 -4.6 1,544,413 + MMP_RS08200 0.35 -5.7 1,544,418 - MMP_RS08200 0.35 -2.3 1,544,465 + MMP_RS08200 0.38 -2.8 1,544,475 + MMP_RS08200 0.39 -4.2 1,544,586 - MMP_RS08200 0.45 -2.6 1,544,606 - MMP_RS08200 0.47 -3.9 1,544,606 - MMP_RS08200 0.47 -4.6 1,544,670 - MMP_RS08200 0.51 -2.7 1,544,676 - MMP_RS08200 0.51 -4.8 1,544,677 + MMP_RS08200 0.51 -4.6 1,544,682 - MMP_RS08200 0.51 -2.3 1,544,682 - MMP_RS08200 0.51 -4.2 1,544,683 + MMP_RS08200 0.51 -4.6 1,544,690 + MMP_RS08200 0.52 -4.2 1,544,690 - MMP_RS08200 0.52 -3.5 1,544,690 - MMP_RS08200 0.52 -3.4 1,544,690 - MMP_RS08200 0.52 -2.6 1,544,691 + MMP_RS08200 0.52 -4.3 1,544,691 + MMP_RS08200 0.52 -3.7 1,544,693 + MMP_RS08200 0.52 -4.7 1,544,693 + MMP_RS08200 0.52 -1.8 1,544,702 + MMP_RS08200 0.53 -5.5 1,544,721 + MMP_RS08200 0.54 -2.8 1,544,723 + MMP_RS08200 0.54 -2.6 1,544,723 + MMP_RS08200 0.54 -3.6 1,544,761 + MMP_RS08200 0.56 -1.8
Or see this region's nucleotide sequence