Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06885 and MMP_RS06890 are separated by 19 nucleotides MMP_RS06890 and MMP_RS06895 are separated by 10 nucleotides MMP_RS06895 and MMP_RS06900 are separated by 106 nucleotides MMP_RS06900 and MMP_RS06905 are separated by 129 nucleotides MMP_RS06905 and MMP_RS06910 are separated by 18 nucleotides
MMP_RS06885: MMP_RS06885 - 50S ribosome-binding GTPase, at 1,314,582 to 1,315,736
_RS06885
MMP_RS06890: MMP_RS06890 - mevalonate kinase, at 1,315,756 to 1,316,709
_RS06890
MMP_RS06895: MMP_RS06895 - selenocysteine-specific translation elongation factor, at 1,316,720 to 1,318,126
_RS06895
MMP_RS06900: MMP_RS06900 - coenzyme F420-reducing hydrogenase FrhD protein, at 1,318,233 to 1,318,715
_RS06900
MMP_RS06905: MMP_RS06905 - hypothetical protein, at 1,318,845 to 1,319,039
_RS06905
MMP_RS06910: MMP_RS06910 - RlmF-related methyltransferase, at 1,319,058 to 1,319,852
_RS06910
Position (kb) 1316
1317
1318
1319 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1316.083 kb on - strand at 1316.104 kb on + strand at 1316.147 kb on + strand at 1316.274 kb on + strand at 1316.539 kb on - strand at 1316.852 kb on + strand at 1317.209 kb on + strand, within MMP_RS06895 at 1317.431 kb on - strand, within MMP_RS06895 at 1317.912 kb on + strand, within MMP_RS06895 at 1317.987 kb on + strand at 1318.163 kb on - strand at 1318.173 kb on + strand at 1318.175 kb on + strand at 1318.176 kb on - strand at 1318.176 kb on - strand at 1318.176 kb on - strand at 1318.176 kb on - strand at 1318.177 kb on + strand at 1318.177 kb on + strand at 1318.177 kb on + strand at 1318.185 kb on + strand at 1318.188 kb on - strand at 1318.189 kb on + strand at 1318.189 kb on + strand at 1318.229 kb on + strand at 1318.286 kb on + strand, within MMP_RS06900 at 1318.557 kb on - strand, within MMP_RS06900 at 1318.561 kb on - strand, within MMP_RS06900 at 1318.562 kb on + strand, within MMP_RS06900 at 1318.592 kb on + strand, within MMP_RS06900 at 1318.753 kb on + strand at 1318.776 kb on - strand at 1318.777 kb on + strand at 1318.893 kb on + strand, within MMP_RS06905 at 1318.973 kb on - strand, within MMP_RS06905 at 1318.974 kb on + strand, within MMP_RS06905 at 1319.033 kb on + strand at 1319.037 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE remove 1,316,083 - -1.9 1,316,104 + -2.3 1,316,147 + -2.3 1,316,274 + -2.1 1,316,539 - -2.4 1,316,852 + -2.4 1,317,209 + MMP_RS06895 0.35 -1.6 1,317,431 - MMP_RS06895 0.51 -4.1 1,317,912 + MMP_RS06895 0.85 -2.5 1,317,987 + -4.0 1,318,163 - -2.8 1,318,173 + -2.5 1,318,175 + -3.3 1,318,176 - -1.1 1,318,176 - -1.4 1,318,176 - -2.5 1,318,176 - -1.5 1,318,177 + -0.5 1,318,177 + -0.4 1,318,177 + -0.6 1,318,185 + -0.4 1,318,188 - -4.0 1,318,189 + -1.2 1,318,189 + -2.8 1,318,229 + -2.7 1,318,286 + MMP_RS06900 0.11 -1.7 1,318,557 - MMP_RS06900 0.67 -3.0 1,318,561 - MMP_RS06900 0.68 -3.9 1,318,562 + MMP_RS06900 0.68 -1.8 1,318,592 + MMP_RS06900 0.74 -3.0 1,318,753 + -0.3 1,318,776 - +0.4 1,318,777 + +0.1 1,318,893 + MMP_RS06905 0.25 +0.3 1,318,973 - MMP_RS06905 0.66 +0.0 1,318,974 + MMP_RS06905 0.66 -0.4 1,319,033 + -0.3 1,319,037 - +0.6
Or see this region's nucleotide sequence