Strain Fitness in Methanococcus maripaludis S2 around MMP_RS06375

Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS06370 and MMP_RS06375 overlap by 4 nucleotidesMMP_RS06375 and MMP_RS06380 are separated by 27 nucleotides MMP_RS06370: MMP_RS06370 - biotin synthase BioB, at 1,223,883 to 1,224,866 _RS06370 MMP_RS06375: MMP_RS06375 - ATP-dependent sacrificial sulfur transferase LarE, at 1,224,863 to 1,225,639 _RS06375 MMP_RS06380: MMP_RS06380 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase, at 1,225,667 to 1,226,809 _RS06380 Position (kb) 1224 1225 1226Strain fitness (log2 ratio) -8 -7 -6 -5 -4 -3 -2 -1 0 1at 1224.010 kb on + strand, within MMP_RS06370at 1224.221 kb on + strand, within MMP_RS06370at 1224.364 kb on - strand, within MMP_RS06370at 1224.417 kb on + strand, within MMP_RS06370at 1224.448 kb on - strand, within MMP_RS06370at 1224.577 kb on - strand, within MMP_RS06370at 1224.897 kb on + strandat 1224.933 kb on - strandat 1224.971 kb on + strand, within MMP_RS06375at 1224.971 kb on + strand, within MMP_RS06375at 1225.246 kb on + strand, within MMP_RS06375at 1225.378 kb on - strand, within MMP_RS06375at 1225.485 kb on + strand, within MMP_RS06375at 1225.570 kb on + strandat 1225.582 kb on - strandat 1225.902 kb on - strandat 1226.409 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE
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1,224,010 + MMP_RS06370 0.13 -3.3
1,224,221 + MMP_RS06370 0.34 -1.2
1,224,364 - MMP_RS06370 0.49 -3.8
1,224,417 + MMP_RS06370 0.54 -3.0
1,224,448 - MMP_RS06370 0.57 -3.1
1,224,577 - MMP_RS06370 0.71 -2.5
1,224,897 + -5.8
1,224,933 - -4.7
1,224,971 + MMP_RS06375 0.14 -5.9
1,224,971 + MMP_RS06375 0.14 -8.2
1,225,246 + MMP_RS06375 0.49 -6.3
1,225,378 - MMP_RS06375 0.66 -5.1
1,225,485 + MMP_RS06375 0.80 -5.8
1,225,570 + -3.3
1,225,582 - -3.4
1,225,902 - -1.0
1,226,409 - -3.0

Or see this region's nucleotide sequence