Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05690 and MMP_RS05695 are separated by 156 nucleotides MMP_RS05695 and MMP_RS05700 overlap by 4 nucleotides MMP_RS05700 and MMP_RS05705 are separated by 49 nucleotides MMP_RS05705 and MMP_RS05710 are separated by 57 nucleotides
MMP_RS05690: MMP_RS05690 - acetylornithine transaminase, at 1,093,257 to 1,094,444
_RS05690
MMP_RS05695: MMP_RS05695 - phospholipase D-like domain-containing protein, at 1,094,601 to 1,095,176
_RS05695
MMP_RS05700: MMP_RS05700 - TfuA-related McrA-glycine thioamidation protein, at 1,095,173 to 1,095,817
_RS05700
MMP_RS05705: MMP_RS05705 - aspartate carbamoyltransferase regulatory subunit, at 1,095,867 to 1,096,313
_RS05705
MMP_RS05710: MMP_RS05710 - ADP-forming succinate--CoA ligase subunit beta, at 1,096,371 to 1,097,459
_RS05710
Position (kb)
1095
1096 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1094.359 kb on + strand at 1094.384 kb on + strand at 1094.433 kb on - strand at 1094.507 kb on - strand at 1094.510 kb on - strand at 1094.510 kb on - strand at 1094.528 kb on - strand at 1094.531 kb on + strand at 1094.643 kb on + strand at 1094.673 kb on - strand, within MMP_RS05695 at 1094.678 kb on + strand, within MMP_RS05695 at 1094.779 kb on + strand, within MMP_RS05695 at 1094.828 kb on - strand, within MMP_RS05695 at 1094.842 kb on - strand, within MMP_RS05695 at 1094.844 kb on - strand, within MMP_RS05695 at 1094.844 kb on - strand, within MMP_RS05695 at 1094.870 kb on + strand, within MMP_RS05695 at 1095.017 kb on - strand, within MMP_RS05695 at 1095.022 kb on - strand, within MMP_RS05695 at 1095.028 kb on - strand, within MMP_RS05695 at 1095.029 kb on + strand, within MMP_RS05695 at 1095.252 kb on - strand, within MMP_RS05700 at 1095.253 kb on + strand, within MMP_RS05700 at 1095.253 kb on + strand, within MMP_RS05700 at 1095.279 kb on - strand, within MMP_RS05700 at 1095.302 kb on - strand, within MMP_RS05700 at 1095.309 kb on - strand, within MMP_RS05700 at 1095.309 kb on - strand, within MMP_RS05700 at 1095.318 kb on - strand, within MMP_RS05700 at 1095.385 kb on - strand, within MMP_RS05700 at 1095.396 kb on - strand, within MMP_RS05700 at 1095.475 kb on - strand, within MMP_RS05700 at 1095.476 kb on + strand, within MMP_RS05700 at 1095.485 kb on + strand, within MMP_RS05700 at 1095.524 kb on + strand, within MMP_RS05700 at 1095.667 kb on - strand, within MMP_RS05700 at 1095.668 kb on + strand, within MMP_RS05700 at 1095.801 kb on + strand at 1095.846 kb on - strand at 1095.846 kb on - strand at 1096.070 kb on + strand at 1096.115 kb on + strand at 1096.115 kb on + strand at 1096.301 kb on + strand at 1096.483 kb on - strand at 1096.653 kb on - strand at 1096.736 kb on + strand at 1096.801 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE remove 1,094,359 + -2.9 1,094,384 + -1.6 1,094,433 - -2.3 1,094,507 - -1.4 1,094,510 - -2.5 1,094,510 - -4.1 1,094,528 - +0.4 1,094,531 + +0.3 1,094,643 + +0.5 1,094,673 - MMP_RS05695 0.12 -0.6 1,094,678 + MMP_RS05695 0.13 -0.4 1,094,779 + MMP_RS05695 0.31 +0.5 1,094,828 - MMP_RS05695 0.39 -0.0 1,094,842 - MMP_RS05695 0.42 +0.3 1,094,844 - MMP_RS05695 0.42 +0.5 1,094,844 - MMP_RS05695 0.42 -0.2 1,094,870 + MMP_RS05695 0.47 +0.5 1,095,017 - MMP_RS05695 0.72 -0.5 1,095,022 - MMP_RS05695 0.73 +1.6 1,095,028 - MMP_RS05695 0.74 +0.1 1,095,029 + MMP_RS05695 0.74 +0.1 1,095,252 - MMP_RS05700 0.12 -1.0 1,095,253 + MMP_RS05700 0.12 -1.8 1,095,253 + MMP_RS05700 0.12 -1.1 1,095,279 - MMP_RS05700 0.16 -0.7 1,095,302 - MMP_RS05700 0.20 -1.8 1,095,309 - MMP_RS05700 0.21 -1.7 1,095,309 - MMP_RS05700 0.21 -1.7 1,095,318 - MMP_RS05700 0.22 -2.9 1,095,385 - MMP_RS05700 0.33 -1.8 1,095,396 - MMP_RS05700 0.35 -3.4 1,095,475 - MMP_RS05700 0.47 -1.8 1,095,476 + MMP_RS05700 0.47 -1.5 1,095,485 + MMP_RS05700 0.48 -1.2 1,095,524 + MMP_RS05700 0.54 -1.9 1,095,667 - MMP_RS05700 0.77 -2.5 1,095,668 + MMP_RS05700 0.77 -1.4 1,095,801 + -0.2 1,095,846 - -2.6 1,095,846 - -2.3 1,096,070 + -3.4 1,096,115 + -3.5 1,096,115 + -2.5 1,096,301 + -0.7 1,096,483 - -3.1 1,096,653 - -3.6 1,096,736 + -1.6 1,096,801 + -2.9
Or see this region's nucleotide sequence