Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05495 and MMP_RS05500 are separated by 83 nucleotides MMP_RS05500 and MMP_RS05505 are separated by 129 nucleotides MMP_RS05505 and MMP_RS05510 overlap by 4 nucleotides
MMP_RS05495: MMP_RS05495 - hypothetical protein, at 1,053,641 to 1,055,482
_RS05495
MMP_RS05500: MMP_RS05500 - 2-isopropylmalate synthase, at 1,055,566 to 1,057,110
_RS05500
MMP_RS05505: MMP_RS05505 - class IV adenylate cyclase, at 1,057,240 to 1,057,803
_RS05505
MMP_RS05510: MMP_RS05510 - Rrf2 family transcriptional regulator, at 1,057,800 to 1,058,234
_RS05510
Position (kb)
1055
1056
1057
1058 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1054.620 kb on - strand, within MMP_RS05495 at 1054.636 kb on - strand, within MMP_RS05495 at 1054.669 kb on + strand, within MMP_RS05495 at 1054.737 kb on - strand, within MMP_RS05495 at 1054.936 kb on + strand, within MMP_RS05495 at 1054.936 kb on + strand, within MMP_RS05495 at 1055.011 kb on + strand, within MMP_RS05495 at 1055.011 kb on + strand, within MMP_RS05495 at 1055.012 kb on - strand, within MMP_RS05495 at 1055.060 kb on - strand, within MMP_RS05495 at 1055.060 kb on - strand, within MMP_RS05495 at 1055.060 kb on - strand, within MMP_RS05495 at 1055.061 kb on + strand, within MMP_RS05495 at 1055.061 kb on + strand, within MMP_RS05495 at 1055.061 kb on + strand, within MMP_RS05495 at 1055.072 kb on + strand, within MMP_RS05495 at 1055.083 kb on + strand, within MMP_RS05495 at 1055.269 kb on + strand, within MMP_RS05495 at 1055.409 kb on - strand at 1055.409 kb on - strand at 1055.414 kb on + strand at 1055.421 kb on + strand at 1055.424 kb on - strand at 1055.424 kb on - strand at 1055.458 kb on - strand at 1055.458 kb on - strand at 1055.459 kb on + strand at 1055.459 kb on + strand at 1055.459 kb on + strand at 1055.459 kb on + strand at 1055.459 kb on + strand at 1055.477 kb on + strand at 1055.523 kb on - strand at 1055.525 kb on - strand at 1055.561 kb on - strand at 1055.561 kb on - strand at 1055.576 kb on + strand at 1055.985 kb on - strand, within MMP_RS05500 at 1055.997 kb on + strand, within MMP_RS05500 at 1056.012 kb on + strand, within MMP_RS05500 at 1056.273 kb on + strand, within MMP_RS05500 at 1056.284 kb on - strand, within MMP_RS05500 at 1056.342 kb on + strand, within MMP_RS05500 at 1056.361 kb on + strand, within MMP_RS05500 at 1056.371 kb on - strand, within MMP_RS05500 at 1056.520 kb on - strand, within MMP_RS05500 at 1056.559 kb on + strand, within MMP_RS05500 at 1056.559 kb on + strand, within MMP_RS05500 at 1056.852 kb on - strand, within MMP_RS05500 at 1057.196 kb on - strand at 1057.203 kb on - strand at 1057.203 kb on - strand at 1057.224 kb on - strand at 1057.241 kb on - strand at 1057.336 kb on - strand, within MMP_RS05505 at 1057.509 kb on + strand, within MMP_RS05505 at 1057.590 kb on - strand, within MMP_RS05505 at 1057.595 kb on - strand, within MMP_RS05505 at 1057.595 kb on - strand, within MMP_RS05505 at 1057.595 kb on - strand, within MMP_RS05505 at 1057.595 kb on - strand, within MMP_RS05505 at 1057.596 kb on + strand, within MMP_RS05505 at 1057.596 kb on + strand, within MMP_RS05505 at 1057.675 kb on - strand, within MMP_RS05505 at 1057.676 kb on + strand, within MMP_RS05505 at 1057.689 kb on + strand, within MMP_RS05505 at 1057.787 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE remove 1,054,620 - MMP_RS05495 0.53 +1.6 1,054,636 - MMP_RS05495 0.54 -0.5 1,054,669 + MMP_RS05495 0.56 +0.6 1,054,737 - MMP_RS05495 0.60 -0.9 1,054,936 + MMP_RS05495 0.70 -0.2 1,054,936 + MMP_RS05495 0.70 -0.1 1,055,011 + MMP_RS05495 0.74 -0.4 1,055,011 + MMP_RS05495 0.74 +0.2 1,055,012 - MMP_RS05495 0.74 -0.8 1,055,060 - MMP_RS05495 0.77 -0.2 1,055,060 - MMP_RS05495 0.77 -0.5 1,055,060 - MMP_RS05495 0.77 -0.2 1,055,061 + MMP_RS05495 0.77 -0.1 1,055,061 + MMP_RS05495 0.77 -0.1 1,055,061 + MMP_RS05495 0.77 -0.6 1,055,072 + MMP_RS05495 0.78 -0.5 1,055,083 + MMP_RS05495 0.78 +0.2 1,055,269 + MMP_RS05495 0.88 -0.9 1,055,409 - -0.3 1,055,409 - -0.5 1,055,414 + -1.7 1,055,421 + -0.3 1,055,424 - -0.6 1,055,424 - -0.4 1,055,458 - +0.1 1,055,458 - +1.2 1,055,459 + -1.8 1,055,459 + -0.0 1,055,459 + +0.3 1,055,459 + -0.0 1,055,459 + +0.2 1,055,477 + -4.0 1,055,523 - -6.7 1,055,525 - -4.1 1,055,561 - -6.1 1,055,561 - -5.8 1,055,576 + -7.1 1,055,985 - MMP_RS05500 0.27 -5.8 1,055,997 + MMP_RS05500 0.28 -5.3 1,056,012 + MMP_RS05500 0.29 -6.0 1,056,273 + MMP_RS05500 0.46 -6.8 1,056,284 - MMP_RS05500 0.46 -5.2 1,056,342 + MMP_RS05500 0.50 -6.4 1,056,361 + MMP_RS05500 0.51 -2.7 1,056,371 - MMP_RS05500 0.52 -6.2 1,056,520 - MMP_RS05500 0.62 -7.0 1,056,559 + MMP_RS05500 0.64 -5.6 1,056,559 + MMP_RS05500 0.64 -6.7 1,056,852 - MMP_RS05500 0.83 -4.9 1,057,196 - -0.0 1,057,203 - -0.9 1,057,203 - +0.2 1,057,224 - +0.3 1,057,241 - +0.1 1,057,336 - MMP_RS05505 0.17 +0.1 1,057,509 + MMP_RS05505 0.48 -0.1 1,057,590 - MMP_RS05505 0.62 -0.1 1,057,595 - MMP_RS05505 0.63 +0.4 1,057,595 - MMP_RS05505 0.63 +0.4 1,057,595 - MMP_RS05505 0.63 +0.3 1,057,595 - MMP_RS05505 0.63 -0.4 1,057,596 + MMP_RS05505 0.63 -0.2 1,057,596 + MMP_RS05505 0.63 +0.6 1,057,675 - MMP_RS05505 0.77 +0.4 1,057,676 + MMP_RS05505 0.77 -0.8 1,057,689 + MMP_RS05505 0.80 +0.2 1,057,787 + +0.0
Or see this region's nucleotide sequence