Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03025 and MMP_RS03030 are separated by 541 nucleotides MMP_RS03030 and MMP_RS03035 are separated by 17 nucleotides MMP_RS03035 and MMP_RS03040 are separated by 9 nucleotides MMP_RS03040 and MMP_RS03045 are separated by 79 nucleotides MMP_RS03045 and MMP_RS03050 are separated by 99 nucleotides
MMP_RS03025: MMP_RS03025 - molybdenum cofactor guanylyltransferase, at 572,518 to 573,162
_RS03025
MMP_RS03030: MMP_RS03030 - Asp-tRNA(Asn) amidotransferase subunit GatC, at 573,704 to 573,943
_RS03030
MMP_RS03035: MMP_RS03035 - 4-hydroxy-tetrahydrodipicolinate synthase, at 573,961 to 574,830
_RS03035
MMP_RS03040: MMP_RS03040 - 30S ribosomal protein S17e, at 574,840 to 575,031
_RS03040
MMP_RS03045: MMP_RS03045 - chorismate mutase, at 575,111 to 575,401
_RS03045
MMP_RS03050: MMP_RS03050 - DUF115 domain-containing protein, at 575,501 to 576,217
_RS03050
Position (kb) 573
574
575 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 572.965 kb on - strand, within MMP_RS03025 at 573.107 kb on - strand at 573.107 kb on - strand at 573.107 kb on - strand at 573.111 kb on - strand at 573.128 kb on - strand at 573.172 kb on - strand at 573.193 kb on + strand at 573.193 kb on + strand at 573.200 kb on + strand at 573.200 kb on + strand at 573.200 kb on + strand at 573.228 kb on - strand at 573.260 kb on - strand at 573.290 kb on - strand at 573.309 kb on - strand at 573.310 kb on + strand at 573.310 kb on + strand at 573.350 kb on + strand at 573.354 kb on - strand at 573.381 kb on - strand at 573.396 kb on - strand at 573.396 kb on - strand at 573.396 kb on - strand at 573.424 kb on - strand at 573.425 kb on + strand at 573.425 kb on + strand at 573.425 kb on + strand at 573.426 kb on - strand at 573.426 kb on - strand at 573.427 kb on + strand at 573.428 kb on - strand at 573.429 kb on + strand at 573.526 kb on - strand at 573.527 kb on + strand at 573.562 kb on + strand at 573.565 kb on - strand at 573.565 kb on - strand at 573.565 kb on - strand at 573.566 kb on + strand at 573.566 kb on + strand at 573.604 kb on + strand at 573.754 kb on - strand at 573.943 kb on - strand at 574.069 kb on + strand, within MMP_RS03035 at 574.291 kb on - strand, within MMP_RS03035 at 574.365 kb on - strand, within MMP_RS03035 at 574.440 kb on + strand, within MMP_RS03035 at 574.441 kb on - strand, within MMP_RS03035 at 574.447 kb on - strand, within MMP_RS03035 at 574.447 kb on - strand, within MMP_RS03035 at 574.518 kb on + strand, within MMP_RS03035 at 575.050 kb on - strand at 575.094 kb on - strand at 575.096 kb on - strand at 575.192 kb on - strand at 575.333 kb on - strand at 575.402 kb on + strand at 575.402 kb on + strand at 575.451 kb on - strand at 575.490 kb on + strand at 575.644 kb on + strand at 575.737 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE remove 572,965 - MMP_RS03025 0.69 -2.3 573,107 - -2.2 573,107 - -3.9 573,107 - -3.4 573,111 - -2.3 573,128 - -3.8 573,172 - +0.3 573,193 + -0.5 573,193 + -0.2 573,200 + -0.4 573,200 + +0.5 573,200 + +0.5 573,228 - +0.2 573,260 - -0.1 573,290 - -0.4 573,309 - -0.2 573,310 + -0.5 573,310 + -0.3 573,350 + -0.4 573,354 - +0.8 573,381 - +0.0 573,396 - +0.0 573,396 - +0.2 573,396 - +0.2 573,424 - -0.0 573,425 + -0.2 573,425 + +0.4 573,425 + +0.3 573,426 - +0.2 573,426 - -0.1 573,427 + +0.9 573,428 - +0.5 573,429 + -0.4 573,526 - -0.1 573,527 + -0.1 573,562 + -0.2 573,565 - -0.2 573,565 - -0.9 573,565 - +1.1 573,566 + +0.2 573,566 + -0.4 573,604 + -1.5 573,754 - -3.5 573,943 - -3.0 574,069 + MMP_RS03035 0.12 -3.4 574,291 - MMP_RS03035 0.38 -2.1 574,365 - MMP_RS03035 0.46 -2.4 574,440 + MMP_RS03035 0.55 -4.7 574,441 - MMP_RS03035 0.55 -3.2 574,447 - MMP_RS03035 0.56 -4.1 574,447 - MMP_RS03035 0.56 -4.3 574,518 + MMP_RS03035 0.64 -2.5 575,050 - -2.4 575,094 - -3.0 575,096 - -3.5 575,192 - -2.1 575,333 - -2.6 575,402 + -1.5 575,402 + -3.6 575,451 - -2.1 575,490 + +1.1 575,644 + -2.1 575,737 + -2.6
Or see this region's nucleotide sequence