Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01910 and MMP_RS01915 are separated by 1 nucleotides MMP_RS01915 and MMP_RS01920 are separated by 9 nucleotides MMP_RS01920 and MMP_RS01925 are separated by 130 nucleotides
MMP_RS01910: MMP_RS01910 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 353,715 to 354,788
_RS01910
MMP_RS01915: MMP_RS01915 - DUF354 domain-containing protein, at 354,790 to 355,911
_RS01915
MMP_RS01920: MMP_RS01920 - glycosyltransferase family 2 protein, at 355,921 to 356,598
_RS01920
MMP_RS01925: MMP_RS01925 - DUF1616 domain-containing protein, at 356,729 to 357,700
_RS01925
Position (kb) 354
355
356 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 353.831 kb on - strand, within MMP_RS01910 at 353.847 kb on - strand, within MMP_RS01910 at 353.848 kb on + strand, within MMP_RS01910 at 353.849 kb on - strand, within MMP_RS01910 at 353.850 kb on + strand, within MMP_RS01910 at 353.850 kb on + strand, within MMP_RS01910 at 353.888 kb on - strand, within MMP_RS01910 at 353.972 kb on - strand, within MMP_RS01910 at 353.984 kb on + strand, within MMP_RS01910 at 353.994 kb on - strand, within MMP_RS01910 at 354.021 kb on - strand, within MMP_RS01910 at 354.027 kb on + strand, within MMP_RS01910 at 354.031 kb on - strand, within MMP_RS01910 at 354.059 kb on + strand, within MMP_RS01910 at 354.066 kb on - strand, within MMP_RS01910 at 354.123 kb on + strand, within MMP_RS01910 at 354.132 kb on - strand, within MMP_RS01910 at 354.135 kb on - strand, within MMP_RS01910 at 354.144 kb on - strand, within MMP_RS01910 at 354.155 kb on - strand, within MMP_RS01910 at 354.190 kb on + strand, within MMP_RS01910 at 354.194 kb on - strand, within MMP_RS01910 at 354.196 kb on - strand, within MMP_RS01910 at 354.197 kb on + strand, within MMP_RS01910 at 354.216 kb on - strand, within MMP_RS01910 at 354.234 kb on + strand, within MMP_RS01910 at 354.281 kb on + strand, within MMP_RS01910 at 354.281 kb on + strand, within MMP_RS01910 at 354.285 kb on - strand, within MMP_RS01910 at 354.285 kb on - strand, within MMP_RS01910 at 354.285 kb on - strand, within MMP_RS01910 at 354.285 kb on - strand, within MMP_RS01910 at 354.286 kb on + strand, within MMP_RS01910 at 354.286 kb on + strand, within MMP_RS01910 at 354.299 kb on - strand, within MMP_RS01910 at 354.335 kb on - strand, within MMP_RS01910 at 354.525 kb on - strand, within MMP_RS01910 at 354.609 kb on + strand, within MMP_RS01910 at 354.676 kb on - strand, within MMP_RS01910 at 354.778 kb on + strand at 354.780 kb on - strand at 354.780 kb on - strand at 354.804 kb on - strand at 354.809 kb on + strand at 354.889 kb on - strand at 354.926 kb on + strand, within MMP_RS01915 at 354.928 kb on - strand, within MMP_RS01915 at 354.928 kb on - strand, within MMP_RS01915 at 354.973 kb on - strand, within MMP_RS01915 at 355.024 kb on - strand, within MMP_RS01915 at 355.024 kb on - strand, within MMP_RS01915 at 355.051 kb on - strand, within MMP_RS01915 at 355.065 kb on - strand, within MMP_RS01915 at 355.091 kb on - strand, within MMP_RS01915 at 355.092 kb on + strand, within MMP_RS01915 at 355.092 kb on + strand, within MMP_RS01915 at 355.092 kb on + strand, within MMP_RS01915 at 355.096 kb on - strand, within MMP_RS01915 at 355.096 kb on - strand, within MMP_RS01915 at 355.111 kb on - strand, within MMP_RS01915 at 355.112 kb on + strand, within MMP_RS01915 at 355.112 kb on + strand, within MMP_RS01915 at 355.167 kb on + strand, within MMP_RS01915 at 355.169 kb on - strand, within MMP_RS01915 at 355.169 kb on - strand, within MMP_RS01915 at 355.169 kb on - strand, within MMP_RS01915 at 355.169 kb on - strand, within MMP_RS01915 at 355.169 kb on - strand, within MMP_RS01915 at 355.170 kb on + strand, within MMP_RS01915 at 355.170 kb on + strand, within MMP_RS01915 at 355.171 kb on - strand, within MMP_RS01915 at 355.171 kb on - strand, within MMP_RS01915 at 355.171 kb on - strand, within MMP_RS01915 at 355.171 kb on - strand, within MMP_RS01915 at 355.172 kb on + strand, within MMP_RS01915 at 355.174 kb on - strand, within MMP_RS01915 at 355.175 kb on + strand, within MMP_RS01915 at 355.175 kb on + strand, within MMP_RS01915 at 355.252 kb on + strand, within MMP_RS01915 at 355.390 kb on - strand, within MMP_RS01915 at 355.390 kb on - strand, within MMP_RS01915 at 355.393 kb on - strand, within MMP_RS01915 at 355.421 kb on - strand, within MMP_RS01915 at 355.422 kb on + strand, within MMP_RS01915 at 355.422 kb on + strand, within MMP_RS01915 at 355.422 kb on + strand, within MMP_RS01915 at 355.425 kb on + strand, within MMP_RS01915 at 355.435 kb on - strand, within MMP_RS01915 at 355.436 kb on + strand, within MMP_RS01915 at 355.440 kb on + strand, within MMP_RS01915 at 355.455 kb on + strand, within MMP_RS01915 at 355.635 kb on + strand, within MMP_RS01915 at 355.654 kb on - strand, within MMP_RS01915 at 355.656 kb on - strand, within MMP_RS01915 at 355.697 kb on + strand, within MMP_RS01915 at 355.708 kb on - strand, within MMP_RS01915 at 355.708 kb on - strand, within MMP_RS01915 at 355.709 kb on + strand, within MMP_RS01915 at 355.751 kb on - strand, within MMP_RS01915 at 355.751 kb on - strand, within MMP_RS01915 at 355.752 kb on + strand, within MMP_RS01915 at 355.753 kb on - strand, within MMP_RS01915 at 355.753 kb on - strand, within MMP_RS01915 at 355.753 kb on - strand, within MMP_RS01915 at 355.754 kb on + strand, within MMP_RS01915 at 355.754 kb on + strand, within MMP_RS01915 at 355.765 kb on - strand, within MMP_RS01915 at 355.772 kb on + strand, within MMP_RS01915 at 355.772 kb on + strand, within MMP_RS01915 at 355.893 kb on - strand at 355.912 kb on + strand at 355.922 kb on - strand at 355.922 kb on - strand at 355.926 kb on + strand at 355.926 kb on + strand at 355.958 kb on - strand at 355.978 kb on - strand at 355.979 kb on + strand at 356.001 kb on - strand, within MMP_RS01920 at 356.002 kb on + strand, within MMP_RS01920 at 356.002 kb on + strand, within MMP_RS01920 at 356.002 kb on + strand, within MMP_RS01920 at 356.016 kb on + strand, within MMP_RS01920 at 356.016 kb on + strand, within MMP_RS01920 at 356.020 kb on - strand, within MMP_RS01920 at 356.020 kb on - strand, within MMP_RS01920 at 356.021 kb on + strand, within MMP_RS01920 at 356.043 kb on - strand, within MMP_RS01920 at 356.044 kb on + strand, within MMP_RS01920 at 356.062 kb on - strand, within MMP_RS01920 at 356.104 kb on + strand, within MMP_RS01920 at 356.111 kb on + strand, within MMP_RS01920 at 356.142 kb on + strand, within MMP_RS01920 at 356.148 kb on - strand, within MMP_RS01920 at 356.149 kb on + strand, within MMP_RS01920 at 356.202 kb on - strand, within MMP_RS01920 at 356.209 kb on - strand, within MMP_RS01920 at 356.210 kb on + strand, within MMP_RS01920 at 356.210 kb on + strand, within MMP_RS01920 at 356.211 kb on - strand, within MMP_RS01920 at 356.211 kb on - strand, within MMP_RS01920 at 356.211 kb on - strand, within MMP_RS01920 at 356.211 kb on - strand, within MMP_RS01920 at 356.211 kb on - strand, within MMP_RS01920 at 356.211 kb on - strand, within MMP_RS01920 at 356.211 kb on - strand, within MMP_RS01920 at 356.212 kb on + strand, within MMP_RS01920 at 356.212 kb on + strand, within MMP_RS01920 at 356.212 kb on + strand, within MMP_RS01920 at 356.212 kb on + strand, within MMP_RS01920 at 356.212 kb on + strand, within MMP_RS01920 at 356.212 kb on + strand, within MMP_RS01920 at 356.213 kb on - strand, within MMP_RS01920 at 356.213 kb on - strand, within MMP_RS01920 at 356.213 kb on - strand, within MMP_RS01920 at 356.213 kb on - strand, within MMP_RS01920 at 356.213 kb on - strand, within MMP_RS01920 at 356.213 kb on - strand, within MMP_RS01920 at 356.214 kb on + strand, within MMP_RS01920 at 356.214 kb on + strand, within MMP_RS01920 at 356.214 kb on + strand, within MMP_RS01920 at 356.215 kb on - strand, within MMP_RS01920 at 356.260 kb on - strand, within MMP_RS01920 at 356.260 kb on - strand, within MMP_RS01920 at 356.282 kb on + strand, within MMP_RS01920 at 356.296 kb on + strand, within MMP_RS01920 at 356.315 kb on + strand, within MMP_RS01920 at 356.358 kb on - strand, within MMP_RS01920 at 356.359 kb on + strand, within MMP_RS01920 at 356.359 kb on + strand, within MMP_RS01920 at 356.359 kb on + strand, within MMP_RS01920 at 356.362 kb on - strand, within MMP_RS01920 at 356.375 kb on - strand, within MMP_RS01920 at 356.382 kb on - strand, within MMP_RS01920 at 356.382 kb on - strand, within MMP_RS01920 at 356.382 kb on - strand, within MMP_RS01920 at 356.383 kb on + strand, within MMP_RS01920 at 356.387 kb on + strand, within MMP_RS01920 at 356.430 kb on - strand, within MMP_RS01920 at 356.430 kb on - strand, within MMP_RS01920 at 356.431 kb on + strand, within MMP_RS01920 at 356.439 kb on - strand, within MMP_RS01920 at 356.440 kb on + strand, within MMP_RS01920 at 356.445 kb on - strand, within MMP_RS01920 at 356.448 kb on - strand, within MMP_RS01920 at 356.493 kb on - strand, within MMP_RS01920 at 356.494 kb on + strand, within MMP_RS01920 at 356.494 kb on + strand, within MMP_RS01920 at 356.505 kb on - strand, within MMP_RS01920 at 356.510 kb on + strand, within MMP_RS01920 at 356.512 kb on - strand, within MMP_RS01920 at 356.513 kb on + strand, within MMP_RS01920 at 356.544 kb on - strand at 356.544 kb on - strand at 356.544 kb on - strand at 356.557 kb on - strand at 356.557 kb on - strand at 356.557 kb on - strand at 356.557 kb on - strand at 356.571 kb on + strand at 356.584 kb on + strand at 356.627 kb on + strand at 356.632 kb on - strand at 356.633 kb on + strand at 356.636 kb on - strand at 356.641 kb on - strand at 356.642 kb on + strand at 356.643 kb on - strand at 356.644 kb on + strand at 356.644 kb on + strand at 356.644 kb on + strand at 356.662 kb on - strand at 356.675 kb on - strand at 356.690 kb on + strand at 356.698 kb on - strand at 356.704 kb on + strand at 356.713 kb on - strand at 356.714 kb on + strand at 356.718 kb on - strand at 356.719 kb on + strand at 356.725 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE remove 353,831 - MMP_RS01910 0.11 -1.1 353,847 - MMP_RS01910 0.12 -1.1 353,848 + MMP_RS01910 0.12 -4.7 353,849 - MMP_RS01910 0.12 -3.9 353,850 + MMP_RS01910 0.13 -4.2 353,850 + MMP_RS01910 0.13 -3.6 353,888 - MMP_RS01910 0.16 -1.5 353,972 - MMP_RS01910 0.24 -2.9 353,984 + MMP_RS01910 0.25 -4.2 353,994 - MMP_RS01910 0.26 -1.1 354,021 - MMP_RS01910 0.28 -2.9 354,027 + MMP_RS01910 0.29 -2.9 354,031 - MMP_RS01910 0.29 -4.3 354,059 + MMP_RS01910 0.32 -2.0 354,066 - MMP_RS01910 0.33 -3.7 354,123 + MMP_RS01910 0.38 -2.8 354,132 - MMP_RS01910 0.39 -3.9 354,135 - MMP_RS01910 0.39 -1.3 354,144 - MMP_RS01910 0.40 -3.9 354,155 - MMP_RS01910 0.41 -3.9 354,190 + MMP_RS01910 0.44 -5.3 354,194 - MMP_RS01910 0.45 -4.9 354,196 - MMP_RS01910 0.45 -3.9 354,197 + MMP_RS01910 0.45 -1.5 354,216 - MMP_RS01910 0.47 -4.7 354,234 + MMP_RS01910 0.48 -3.4 354,281 + MMP_RS01910 0.53 -3.7 354,281 + MMP_RS01910 0.53 -2.6 354,285 - MMP_RS01910 0.53 -4.0 354,285 - MMP_RS01910 0.53 +0.5 354,285 - MMP_RS01910 0.53 -4.1 354,285 - MMP_RS01910 0.53 -2.3 354,286 + MMP_RS01910 0.53 -1.7 354,286 + MMP_RS01910 0.53 -3.5 354,299 - MMP_RS01910 0.54 -0.5 354,335 - MMP_RS01910 0.58 -5.3 354,525 - MMP_RS01910 0.75 -3.4 354,609 + MMP_RS01910 0.83 -3.5 354,676 - MMP_RS01910 0.89 -4.3 354,778 + -3.3 354,780 - -2.6 354,780 - -5.5 354,804 - -4.6 354,809 + -4.0 354,889 - -3.7 354,926 + MMP_RS01915 0.12 -5.0 354,928 - MMP_RS01915 0.12 -4.1 354,928 - MMP_RS01915 0.12 -4.1 354,973 - MMP_RS01915 0.16 -1.7 355,024 - MMP_RS01915 0.21 -2.2 355,024 - MMP_RS01915 0.21 -2.6 355,051 - MMP_RS01915 0.23 -4.3 355,065 - MMP_RS01915 0.25 -2.4 355,091 - MMP_RS01915 0.27 -4.8 355,092 + MMP_RS01915 0.27 -3.5 355,092 + MMP_RS01915 0.27 -3.5 355,092 + MMP_RS01915 0.27 -1.8 355,096 - MMP_RS01915 0.27 -2.9 355,096 - MMP_RS01915 0.27 -2.9 355,111 - MMP_RS01915 0.29 -3.3 355,112 + MMP_RS01915 0.29 -3.3 355,112 + MMP_RS01915 0.29 -3.9 355,167 + MMP_RS01915 0.34 -2.0 355,169 - MMP_RS01915 0.34 -3.6 355,169 - MMP_RS01915 0.34 -3.9 355,169 - MMP_RS01915 0.34 -4.6 355,169 - MMP_RS01915 0.34 -2.5 355,169 - MMP_RS01915 0.34 -5.0 355,170 + MMP_RS01915 0.34 -4.2 355,170 + MMP_RS01915 0.34 -2.7 355,171 - MMP_RS01915 0.34 -3.7 355,171 - MMP_RS01915 0.34 -2.8 355,171 - MMP_RS01915 0.34 -2.5 355,171 - MMP_RS01915 0.34 -3.7 355,172 + MMP_RS01915 0.34 -4.2 355,174 - MMP_RS01915 0.34 -4.4 355,175 + MMP_RS01915 0.34 -2.4 355,175 + MMP_RS01915 0.34 -3.2 355,252 + MMP_RS01915 0.41 -3.8 355,390 - MMP_RS01915 0.53 -3.7 355,390 - MMP_RS01915 0.53 -0.5 355,393 - MMP_RS01915 0.54 -4.1 355,421 - MMP_RS01915 0.56 -4.4 355,422 + MMP_RS01915 0.56 -4.4 355,422 + MMP_RS01915 0.56 -2.4 355,422 + MMP_RS01915 0.56 -3.8 355,425 + MMP_RS01915 0.57 +0.5 355,435 - MMP_RS01915 0.57 -3.2 355,436 + MMP_RS01915 0.58 -3.1 355,440 + MMP_RS01915 0.58 -3.1 355,455 + MMP_RS01915 0.59 -2.1 355,635 + MMP_RS01915 0.75 -2.4 355,654 - MMP_RS01915 0.77 -4.5 355,656 - MMP_RS01915 0.77 -2.7 355,697 + MMP_RS01915 0.81 -3.2 355,708 - MMP_RS01915 0.82 -4.6 355,708 - MMP_RS01915 0.82 -3.1 355,709 + MMP_RS01915 0.82 -4.0 355,751 - MMP_RS01915 0.86 -3.5 355,751 - MMP_RS01915 0.86 -2.3 355,752 + MMP_RS01915 0.86 -2.2 355,753 - MMP_RS01915 0.86 -4.9 355,753 - MMP_RS01915 0.86 -4.2 355,753 - MMP_RS01915 0.86 -4.4 355,754 + MMP_RS01915 0.86 -4.1 355,754 + MMP_RS01915 0.86 -3.5 355,765 - MMP_RS01915 0.87 -3.5 355,772 + MMP_RS01915 0.88 -4.2 355,772 + MMP_RS01915 0.88 -3.4 355,893 - -4.9 355,912 + -3.0 355,922 - -2.0 355,922 - -3.7 355,926 + -3.1 355,926 + -3.1 355,958 - -5.0 355,978 - -4.1 355,979 + -3.9 356,001 - MMP_RS01920 0.12 +0.5 356,002 + MMP_RS01920 0.12 -2.9 356,002 + MMP_RS01920 0.12 -2.9 356,002 + MMP_RS01920 0.12 -2.5 356,016 + MMP_RS01920 0.14 -6.6 356,016 + MMP_RS01920 0.14 -4.7 356,020 - MMP_RS01920 0.15 -3.0 356,020 - MMP_RS01920 0.15 -3.6 356,021 + MMP_RS01920 0.15 -2.5 356,043 - MMP_RS01920 0.18 -2.5 356,044 + MMP_RS01920 0.18 -3.9 356,062 - MMP_RS01920 0.21 -3.8 356,104 + MMP_RS01920 0.27 -4.0 356,111 + MMP_RS01920 0.28 -1.3 356,142 + MMP_RS01920 0.33 -2.7 356,148 - MMP_RS01920 0.33 -3.8 356,149 + MMP_RS01920 0.34 -2.9 356,202 - MMP_RS01920 0.41 -2.9 356,209 - MMP_RS01920 0.42 -4.0 356,210 + MMP_RS01920 0.43 -5.4 356,210 + MMP_RS01920 0.43 -3.5 356,211 - MMP_RS01920 0.43 -4.7 356,211 - MMP_RS01920 0.43 -3.5 356,211 - MMP_RS01920 0.43 -3.4 356,211 - MMP_RS01920 0.43 -4.2 356,211 - MMP_RS01920 0.43 -5.2 356,211 - MMP_RS01920 0.43 -2.5 356,211 - MMP_RS01920 0.43 -4.5 356,212 + MMP_RS01920 0.43 -3.9 356,212 + MMP_RS01920 0.43 +1.4 356,212 + MMP_RS01920 0.43 -5.1 356,212 + MMP_RS01920 0.43 -4.9 356,212 + MMP_RS01920 0.43 -4.5 356,212 + MMP_RS01920 0.43 -4.4 356,213 - MMP_RS01920 0.43 -4.3 356,213 - MMP_RS01920 0.43 -4.3 356,213 - MMP_RS01920 0.43 -4.5 356,213 - MMP_RS01920 0.43 -3.8 356,213 - MMP_RS01920 0.43 -3.7 356,213 - MMP_RS01920 0.43 -4.2 356,214 + MMP_RS01920 0.43 -4.0 356,214 + MMP_RS01920 0.43 -2.0 356,214 + MMP_RS01920 0.43 -1.0 356,215 - MMP_RS01920 0.43 -3.9 356,260 - MMP_RS01920 0.50 -3.7 356,260 - MMP_RS01920 0.50 -4.2 356,282 + MMP_RS01920 0.53 -3.3 356,296 + MMP_RS01920 0.55 -1.7 356,315 + MMP_RS01920 0.58 -3.7 356,358 - MMP_RS01920 0.64 -2.9 356,359 + MMP_RS01920 0.65 -6.4 356,359 + MMP_RS01920 0.65 -3.8 356,359 + MMP_RS01920 0.65 -3.9 356,362 - MMP_RS01920 0.65 -4.7 356,375 - MMP_RS01920 0.67 -2.7 356,382 - MMP_RS01920 0.68 -3.7 356,382 - MMP_RS01920 0.68 -4.0 356,382 - MMP_RS01920 0.68 -3.6 356,383 + MMP_RS01920 0.68 -3.4 356,387 + MMP_RS01920 0.69 -1.4 356,430 - MMP_RS01920 0.75 -5.0 356,430 - MMP_RS01920 0.75 -1.7 356,431 + MMP_RS01920 0.75 -4.5 356,439 - MMP_RS01920 0.76 -4.8 356,440 + MMP_RS01920 0.77 -4.2 356,445 - MMP_RS01920 0.77 -3.0 356,448 - MMP_RS01920 0.78 -4.5 356,493 - MMP_RS01920 0.84 -4.2 356,494 + MMP_RS01920 0.85 -3.5 356,494 + MMP_RS01920 0.85 -3.7 356,505 - MMP_RS01920 0.86 -3.9 356,510 + MMP_RS01920 0.87 -4.7 356,512 - MMP_RS01920 0.87 -2.9 356,513 + MMP_RS01920 0.87 -4.3 356,544 - -5.5 356,544 - -2.4 356,544 - -3.9 356,557 - -1.9 356,557 - -3.4 356,557 - -2.9 356,557 - -5.0 356,571 + -1.9 356,584 + -1.7 356,627 + -4.3 356,632 - -4.2 356,633 + -4.8 356,636 - -1.7 356,641 - -3.3 356,642 + -4.8 356,643 - -4.9 356,644 + -4.3 356,644 + -3.4 356,644 + -0.4 356,662 - +0.4 356,675 - +1.7 356,690 + +0.2 356,698 - -0.6 356,704 + -2.6 356,713 - +0.7 356,714 + -4.7 356,718 - -3.2 356,719 + -5.2 356,725 - -1.7
Or see this region's nucleotide sequence