Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00885 and MMP_RS00890 are separated by 25 nucleotides MMP_RS00890 and MMP_RS00895 are separated by 0 nucleotides MMP_RS00895 and MMP_RS00900 are separated by 16 nucleotides MMP_RS00900 and MMP_RS00905 are separated by 71 nucleotides
MMP_RS00885: MMP_RS00885 - 30S ribosomal protein S19e, at 167,490 to 167,915
_RS00885
MMP_RS00890: MMP_RS00890 - DNA-binding protein, at 167,941 to 168,300
_RS00890
MMP_RS00895: MMP_RS00895 - hypothetical protein, at 168,301 to 168,900
_RS00895
MMP_RS00900: MMP_RS00900 - 50S ribosomal protein L39e, at 168,917 to 169,072
_RS00900
MMP_RS00905: MMP_RS00905 - phenylacetate--CoA ligase, at 169,144 to 170,445
_RS00905
Position (kb) 168
169 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 167.906 kb on + strand at 168.288 kb on + strand at 168.527 kb on - strand, within MMP_RS00895 at 168.740 kb on - strand, within MMP_RS00895 at 168.740 kb on - strand, within MMP_RS00895 at 168.741 kb on + strand, within MMP_RS00895 at 168.752 kb on + strand, within MMP_RS00895 at 168.758 kb on - strand, within MMP_RS00895 at 168.988 kb on - strand, within MMP_RS00900 at 169.021 kb on - strand, within MMP_RS00900 at 169.560 kb on - strand, within MMP_RS00905 at 169.651 kb on - strand, within MMP_RS00905
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE remove 167,906 + -4.8 168,288 + -4.4 168,527 - MMP_RS00895 0.38 -3.6 168,740 - MMP_RS00895 0.73 -3.5 168,740 - MMP_RS00895 0.73 -5.4 168,741 + MMP_RS00895 0.73 -5.2 168,752 + MMP_RS00895 0.75 -4.4 168,758 - MMP_RS00895 0.76 -0.5 168,988 - MMP_RS00900 0.46 +0.2 169,021 - MMP_RS00900 0.67 -1.4 169,560 - MMP_RS00905 0.32 +0.4 169,651 - MMP_RS00905 0.39 +0.2
Or see this region's nucleotide sequence