Experiment: Recovery broth with Penicillin G 1e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13940 and BBR_RS13945 are separated by 168 nucleotides BBR_RS13945 and BBR_RS13950 are separated by 1 nucleotides BBR_RS13950 and BBR_RS20810 are separated by 108 nucleotides BBR_RS20810 and BBR_RS13955 are separated by 9 nucleotides BBR_RS13955 and BBR_RS13960 are separated by 439 nucleotides BBR_RS13960 and BBR_RS13965 are separated by 11 nucleotides
BBR_RS13940: BBR_RS13940 - integrase, at 906,789 to 907,040
_RS13940
BBR_RS13945: BBR_RS13945 - Txe/YoeB family addiction module toxin, at 907,209 to 907,484
_RS13945
BBR_RS13950: BBR_RS13950 - type II toxin-antitoxin system antitoxin, RelB/DinJ family, at 907,486 to 907,740
_RS13950
BBR_RS20810: BBR_RS20810 - pirin, at 907,849 to 907,959
_RS20810
BBR_RS13955: BBR_RS13955 - argininosuccinate lyase, at 907,969 to 909,441
_RS13955
BBR_RS13960: BBR_RS13960 - thiamine biosynthesis protein ThiS, at 909,881 to 910,075
_RS13960
BBR_RS13965: BBR_RS13965 - thiazole synthase, at 910,087 to 910,956
_RS13965
Position (kb) 907
908
909
910 Strain fitness (log2 ratio) -2
-1
0
1
2 at 906.989 kb on + strand, within BBR_RS13940 at 907.005 kb on + strand, within BBR_RS13940 at 907.072 kb on - strand at 907.078 kb on - strand at 907.078 kb on - strand at 907.153 kb on - strand at 907.155 kb on - strand at 907.158 kb on - strand at 907.158 kb on - strand at 907.174 kb on - strand at 907.183 kb on + strand at 907.184 kb on - strand at 907.207 kb on + strand at 907.207 kb on + strand at 907.207 kb on + strand at 907.207 kb on + strand at 907.214 kb on + strand at 907.215 kb on - strand at 907.215 kb on - strand at 907.234 kb on - strand at 907.307 kb on + strand, within BBR_RS13945 at 907.350 kb on - strand, within BBR_RS13945 at 907.360 kb on - strand, within BBR_RS13945 at 907.434 kb on + strand, within BBR_RS13945 at 907.479 kb on + strand at 907.904 kb on + strand, within BBR_RS20810 at 907.920 kb on + strand, within BBR_RS20810 at 907.920 kb on + strand, within BBR_RS20810 at 907.953 kb on + strand at 907.953 kb on + strand at 907.961 kb on - strand at 907.977 kb on + strand at 907.995 kb on - strand at 908.037 kb on + strand at 908.046 kb on + strand at 908.046 kb on + strand at 908.054 kb on - strand at 908.054 kb on - strand at 908.054 kb on - strand at 908.109 kb on + strand at 908.116 kb on + strand at 908.116 kb on + strand at 908.141 kb on + strand, within BBR_RS13955 at 908.144 kb on - strand, within BBR_RS13955 at 908.152 kb on + strand, within BBR_RS13955 at 908.165 kb on - strand, within BBR_RS13955 at 908.182 kb on + strand, within BBR_RS13955 at 908.182 kb on + strand, within BBR_RS13955 at 908.182 kb on + strand, within BBR_RS13955 at 908.190 kb on - strand, within BBR_RS13955 at 908.207 kb on + strand, within BBR_RS13955 at 908.247 kb on + strand, within BBR_RS13955 at 908.281 kb on + strand, within BBR_RS13955 at 908.401 kb on + strand, within BBR_RS13955 at 908.402 kb on + strand, within BBR_RS13955 at 908.457 kb on + strand, within BBR_RS13955 at 908.496 kb on - strand, within BBR_RS13955 at 908.561 kb on + strand, within BBR_RS13955 at 908.569 kb on + strand, within BBR_RS13955 at 908.584 kb on - strand, within BBR_RS13955 at 908.592 kb on + strand, within BBR_RS13955 at 908.606 kb on + strand, within BBR_RS13955 at 908.681 kb on + strand, within BBR_RS13955 at 908.681 kb on + strand, within BBR_RS13955 at 908.735 kb on + strand, within BBR_RS13955 at 908.778 kb on - strand, within BBR_RS13955 at 908.784 kb on - strand, within BBR_RS13955 at 908.916 kb on + strand, within BBR_RS13955 at 908.916 kb on + strand, within BBR_RS13955 at 908.922 kb on + strand, within BBR_RS13955 at 908.922 kb on + strand, within BBR_RS13955 at 908.923 kb on + strand, within BBR_RS13955 at 908.923 kb on + strand, within BBR_RS13955 at 908.929 kb on + strand, within BBR_RS13955 at 908.971 kb on - strand, within BBR_RS13955 at 908.999 kb on - strand, within BBR_RS13955 at 909.024 kb on + strand, within BBR_RS13955 at 909.024 kb on + strand, within BBR_RS13955 at 909.024 kb on + strand, within BBR_RS13955 at 909.032 kb on - strand, within BBR_RS13955 at 909.032 kb on - strand, within BBR_RS13955 at 909.032 kb on - strand, within BBR_RS13955 at 909.032 kb on - strand, within BBR_RS13955 at 909.032 kb on - strand, within BBR_RS13955 at 909.096 kb on - strand, within BBR_RS13955 at 909.120 kb on - strand, within BBR_RS13955 at 909.139 kb on + strand, within BBR_RS13955 at 909.158 kb on - strand, within BBR_RS13955 at 909.161 kb on + strand, within BBR_RS13955 at 909.179 kb on + strand, within BBR_RS13955 at 909.179 kb on + strand, within BBR_RS13955 at 909.187 kb on + strand, within BBR_RS13955 at 909.251 kb on + strand, within BBR_RS13955 at 909.274 kb on - strand, within BBR_RS13955 at 909.325 kb on + strand at 909.325 kb on + strand at 909.333 kb on - strand at 909.333 kb on - strand at 909.401 kb on + strand at 909.415 kb on + strand at 909.435 kb on + strand at 909.435 kb on + strand at 909.499 kb on + strand at 909.519 kb on + strand at 909.519 kb on + strand at 909.533 kb on - strand at 909.593 kb on - strand at 909.593 kb on - strand at 909.593 kb on - strand at 909.593 kb on - strand at 909.769 kb on + strand at 909.827 kb on + strand at 909.863 kb on - strand at 909.886 kb on + strand at 909.886 kb on + strand at 909.894 kb on - strand at 909.894 kb on - strand at 909.924 kb on + strand, within BBR_RS13960 at 909.932 kb on - strand, within BBR_RS13960 at 910.056 kb on + strand at 910.094 kb on + strand at 910.167 kb on + strand at 910.167 kb on + strand at 910.190 kb on - strand, within BBR_RS13965 at 910.192 kb on + strand, within BBR_RS13965 at 910.264 kb on + strand, within BBR_RS13965 at 910.272 kb on - strand, within BBR_RS13965 at 910.272 kb on - strand, within BBR_RS13965 at 910.275 kb on + strand, within BBR_RS13965 at 910.341 kb on - strand, within BBR_RS13965 at 910.342 kb on + strand, within BBR_RS13965 at 910.342 kb on + strand, within BBR_RS13965 at 910.350 kb on - strand, within BBR_RS13965 at 910.350 kb on - strand, within BBR_RS13965 at 910.350 kb on - strand, within BBR_RS13965 at 910.350 kb on - strand, within BBR_RS13965 at 910.395 kb on - strand, within BBR_RS13965 at 910.397 kb on - strand, within BBR_RS13965 at 910.405 kb on - strand, within BBR_RS13965 at 910.405 kb on - strand, within BBR_RS13965 at 910.420 kb on - strand, within BBR_RS13965 at 910.420 kb on - strand, within BBR_RS13965 at 910.441 kb on - strand, within BBR_RS13965
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Penicillin G 1e-04 mM remove 906,989 + BBR_RS13940 0.79 +1.3 907,005 + BBR_RS13940 0.86 -0.1 907,072 - -1.4 907,078 - +0.1 907,078 - +0.6 907,153 - +0.2 907,155 - -1.3 907,158 - -0.2 907,158 - +0.6 907,174 - -0.2 907,183 + +0.3 907,184 - +0.1 907,207 + -0.2 907,207 + +0.2 907,207 + +0.7 907,207 + +0.9 907,214 + -0.1 907,215 - -0.0 907,215 - -0.2 907,234 - +0.5 907,307 + BBR_RS13945 0.36 -0.2 907,350 - BBR_RS13945 0.51 +0.9 907,360 - BBR_RS13945 0.55 +0.4 907,434 + BBR_RS13945 0.82 -0.2 907,479 + +0.1 907,904 + BBR_RS20810 0.50 +0.4 907,920 + BBR_RS20810 0.64 -0.7 907,920 + BBR_RS20810 0.64 -0.5 907,953 + -1.1 907,953 + +0.5 907,961 - -0.0 907,977 + -0.3 907,995 - +0.6 908,037 + +0.2 908,046 + +0.5 908,046 + +0.5 908,054 - -0.3 908,054 - +0.5 908,054 - +0.3 908,109 + -1.6 908,116 + +0.4 908,116 + -0.3 908,141 + BBR_RS13955 0.12 +1.4 908,144 - BBR_RS13955 0.12 +1.0 908,152 + BBR_RS13955 0.12 -0.8 908,165 - BBR_RS13955 0.13 +0.7 908,182 + BBR_RS13955 0.14 -2.5 908,182 + BBR_RS13955 0.14 -0.2 908,182 + BBR_RS13955 0.14 +0.2 908,190 - BBR_RS13955 0.15 -1.2 908,207 + BBR_RS13955 0.16 +0.6 908,247 + BBR_RS13955 0.19 +0.1 908,281 + BBR_RS13955 0.21 +0.0 908,401 + BBR_RS13955 0.29 +0.4 908,402 + BBR_RS13955 0.29 +1.2 908,457 + BBR_RS13955 0.33 +0.5 908,496 - BBR_RS13955 0.36 +1.3 908,561 + BBR_RS13955 0.40 -1.4 908,569 + BBR_RS13955 0.41 -1.1 908,584 - BBR_RS13955 0.42 -0.1 908,592 + BBR_RS13955 0.42 -1.3 908,606 + BBR_RS13955 0.43 +0.3 908,681 + BBR_RS13955 0.48 +1.0 908,681 + BBR_RS13955 0.48 +0.6 908,735 + BBR_RS13955 0.52 +0.4 908,778 - BBR_RS13955 0.55 +1.4 908,784 - BBR_RS13955 0.55 -1.5 908,916 + BBR_RS13955 0.64 +0.4 908,916 + BBR_RS13955 0.64 +0.6 908,922 + BBR_RS13955 0.65 +1.2 908,922 + BBR_RS13955 0.65 +1.3 908,923 + BBR_RS13955 0.65 -0.1 908,923 + BBR_RS13955 0.65 -0.1 908,929 + BBR_RS13955 0.65 +0.4 908,971 - BBR_RS13955 0.68 -0.3 908,999 - BBR_RS13955 0.70 +0.5 909,024 + BBR_RS13955 0.72 +0.3 909,024 + BBR_RS13955 0.72 -0.6 909,024 + BBR_RS13955 0.72 -0.8 909,032 - BBR_RS13955 0.72 +1.2 909,032 - BBR_RS13955 0.72 +1.7 909,032 - BBR_RS13955 0.72 -0.1 909,032 - BBR_RS13955 0.72 -0.3 909,032 - BBR_RS13955 0.72 +0.6 909,096 - BBR_RS13955 0.77 -0.9 909,120 - BBR_RS13955 0.78 +0.7 909,139 + BBR_RS13955 0.79 +1.3 909,158 - BBR_RS13955 0.81 +1.2 909,161 + BBR_RS13955 0.81 -0.4 909,179 + BBR_RS13955 0.82 -0.7 909,179 + BBR_RS13955 0.82 -0.1 909,187 + BBR_RS13955 0.83 +0.0 909,251 + BBR_RS13955 0.87 +0.3 909,274 - BBR_RS13955 0.89 -0.8 909,325 + +0.6 909,325 + +0.2 909,333 - +0.6 909,333 - +0.8 909,401 + +1.0 909,415 + -0.8 909,435 + +0.3 909,435 + +0.1 909,499 + +0.4 909,519 + +0.3 909,519 + -0.2 909,533 - +2.7 909,593 - +0.1 909,593 - +0.4 909,593 - -0.2 909,593 - -1.8 909,769 + +0.4 909,827 + +0.7 909,863 - +0.5 909,886 + +0.2 909,886 + +0.4 909,894 - +0.7 909,894 - -0.3 909,924 + BBR_RS13960 0.22 -0.1 909,932 - BBR_RS13960 0.26 -0.4 910,056 + +0.2 910,094 + -2.7 910,167 + -0.5 910,167 + -0.1 910,190 - BBR_RS13965 0.12 +1.0 910,192 + BBR_RS13965 0.12 +0.5 910,264 + BBR_RS13965 0.20 +0.6 910,272 - BBR_RS13965 0.21 +0.1 910,272 - BBR_RS13965 0.21 +0.5 910,275 + BBR_RS13965 0.22 +0.1 910,341 - BBR_RS13965 0.29 -0.1 910,342 + BBR_RS13965 0.29 +0.6 910,342 + BBR_RS13965 0.29 +0.8 910,350 - BBR_RS13965 0.30 +0.4 910,350 - BBR_RS13965 0.30 +0.6 910,350 - BBR_RS13965 0.30 -1.5 910,350 - BBR_RS13965 0.30 -0.1 910,395 - BBR_RS13965 0.35 +2.0 910,397 - BBR_RS13965 0.36 -1.1 910,405 - BBR_RS13965 0.37 -0.3 910,405 - BBR_RS13965 0.37 -1.4 910,420 - BBR_RS13965 0.38 +1.2 910,420 - BBR_RS13965 0.38 -0.9 910,441 - BBR_RS13965 0.41 +0.2
Or see this region's nucleotide sequence