Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_860 and Pf6N2E2_861 are separated by 123 nucleotides Pf6N2E2_861 and Pf6N2E2_862 are separated by 84 nucleotides
Pf6N2E2_860 - Altronate dehydratase (EC 4.2.1.7), at 1,007,678 to 1,009,225
_860
Pf6N2E2_861 - Transcriptional regulator, LysR family, at 1,009,349 to 1,010,371
_861
Pf6N2E2_862 - Fumarylacetoacetate hydrolase family protein, at 1,010,456 to 1,011,634
_862
Position (kb)
1009
1010
1011 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1008.359 kb on - strand, within Pf6N2E2_860 at 1008.370 kb on - strand, within Pf6N2E2_860 at 1008.499 kb on + strand, within Pf6N2E2_860 at 1008.499 kb on + strand, within Pf6N2E2_860 at 1008.500 kb on - strand, within Pf6N2E2_860 at 1008.535 kb on + strand, within Pf6N2E2_860 at 1008.567 kb on - strand, within Pf6N2E2_860 at 1008.567 kb on - strand at 1008.826 kb on + strand, within Pf6N2E2_860 at 1008.826 kb on + strand, within Pf6N2E2_860 at 1008.826 kb on + strand, within Pf6N2E2_860 at 1008.826 kb on + strand, within Pf6N2E2_860 at 1008.827 kb on - strand, within Pf6N2E2_860 at 1008.841 kb on - strand, within Pf6N2E2_860 at 1008.841 kb on - strand, within Pf6N2E2_860 at 1008.841 kb on - strand, within Pf6N2E2_860 at 1008.841 kb on - strand, within Pf6N2E2_860 at 1008.844 kb on + strand, within Pf6N2E2_860 at 1008.940 kb on - strand, within Pf6N2E2_860 at 1008.940 kb on - strand, within Pf6N2E2_860 at 1008.940 kb on - strand, within Pf6N2E2_860 at 1008.998 kb on + strand, within Pf6N2E2_860 at 1008.999 kb on - strand, within Pf6N2E2_860 at 1009.078 kb on + strand at 1009.079 kb on - strand at 1009.266 kb on - strand at 1009.292 kb on + strand at 1009.292 kb on + strand at 1009.293 kb on - strand at 1009.293 kb on - strand at 1009.293 kb on - strand at 1009.293 kb on - strand at 1009.315 kb on + strand at 1009.339 kb on + strand at 1009.432 kb on + strand at 1009.485 kb on + strand, within Pf6N2E2_861 at 1009.699 kb on + strand, within Pf6N2E2_861 at 1009.699 kb on + strand, within Pf6N2E2_861 at 1009.710 kb on + strand, within Pf6N2E2_861 at 1009.710 kb on + strand, within Pf6N2E2_861 at 1009.907 kb on - strand, within Pf6N2E2_861 at 1009.930 kb on + strand, within Pf6N2E2_861 at 1009.931 kb on - strand, within Pf6N2E2_861 at 1009.955 kb on + strand, within Pf6N2E2_861 at 1009.956 kb on - strand, within Pf6N2E2_861 at 1010.002 kb on + strand, within Pf6N2E2_861 at 1010.083 kb on + strand, within Pf6N2E2_861 at 1010.084 kb on - strand, within Pf6N2E2_861 at 1010.086 kb on + strand, within Pf6N2E2_861 at 1010.263 kb on + strand, within Pf6N2E2_861 at 1010.264 kb on - strand, within Pf6N2E2_861 at 1010.273 kb on + strand at 1010.401 kb on - strand at 1010.416 kb on + strand at 1010.417 kb on - strand at 1010.422 kb on + strand at 1010.676 kb on - strand, within Pf6N2E2_862 at 1010.676 kb on - strand, within Pf6N2E2_862 at 1010.935 kb on + strand, within Pf6N2E2_862 at 1010.959 kb on + strand, within Pf6N2E2_862 at 1010.959 kb on + strand, within Pf6N2E2_862 at 1010.960 kb on - strand, within Pf6N2E2_862 at 1010.960 kb on - strand, within Pf6N2E2_862 at 1010.972 kb on + strand, within Pf6N2E2_862
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 1,008,359 - Pf6N2E2_860 0.44 +0.1 1,008,370 - Pf6N2E2_860 0.45 -1.7 1,008,499 + Pf6N2E2_860 0.53 -0.3 1,008,499 + Pf6N2E2_860 0.53 -0.5 1,008,500 - Pf6N2E2_860 0.53 -0.5 1,008,535 + Pf6N2E2_860 0.55 +0.3 1,008,567 - Pf6N2E2_860 0.57 +0.5 1,008,567 - +1.9 1,008,826 + Pf6N2E2_860 0.74 -0.8 1,008,826 + Pf6N2E2_860 0.74 -1.7 1,008,826 + Pf6N2E2_860 0.74 -0.1 1,008,826 + Pf6N2E2_860 0.74 -0.4 1,008,827 - Pf6N2E2_860 0.74 +0.2 1,008,841 - Pf6N2E2_860 0.75 -3.5 1,008,841 - Pf6N2E2_860 0.75 +0.9 1,008,841 - Pf6N2E2_860 0.75 -0.8 1,008,841 - Pf6N2E2_860 0.75 +0.2 1,008,844 + Pf6N2E2_860 0.75 +0.3 1,008,940 - Pf6N2E2_860 0.82 +0.9 1,008,940 - Pf6N2E2_860 0.82 +0.2 1,008,940 - Pf6N2E2_860 0.82 -1.0 1,008,998 + Pf6N2E2_860 0.85 +0.4 1,008,999 - Pf6N2E2_860 0.85 +0.7 1,009,078 + -0.1 1,009,079 - +0.6 1,009,266 - +2.1 1,009,292 + -1.0 1,009,292 + +0.0 1,009,293 - +0.4 1,009,293 - +1.5 1,009,293 - -0.0 1,009,293 - +1.2 1,009,315 + -0.7 1,009,339 + -1.0 1,009,432 + -3.1 1,009,485 + Pf6N2E2_861 0.13 -2.0 1,009,699 + Pf6N2E2_861 0.34 -1.4 1,009,699 + Pf6N2E2_861 0.34 -1.0 1,009,710 + Pf6N2E2_861 0.35 -0.6 1,009,710 + Pf6N2E2_861 0.35 -0.6 1,009,907 - Pf6N2E2_861 0.55 +0.0 1,009,930 + Pf6N2E2_861 0.57 -0.0 1,009,931 - Pf6N2E2_861 0.57 -1.6 1,009,955 + Pf6N2E2_861 0.59 -0.5 1,009,956 - Pf6N2E2_861 0.59 -1.0 1,010,002 + Pf6N2E2_861 0.64 +0.1 1,010,083 + Pf6N2E2_861 0.72 +0.5 1,010,084 - Pf6N2E2_861 0.72 -2.0 1,010,086 + Pf6N2E2_861 0.72 -0.1 1,010,263 + Pf6N2E2_861 0.89 +0.2 1,010,264 - Pf6N2E2_861 0.89 -0.9 1,010,273 + -0.4 1,010,401 - -0.1 1,010,416 + +0.2 1,010,417 - +0.0 1,010,422 + -0.2 1,010,676 - Pf6N2E2_862 0.19 -0.5 1,010,676 - Pf6N2E2_862 0.19 -2.1 1,010,935 + Pf6N2E2_862 0.41 +1.0 1,010,959 + Pf6N2E2_862 0.43 +0.1 1,010,959 + Pf6N2E2_862 0.43 +1.5 1,010,960 - Pf6N2E2_862 0.43 +0.6 1,010,960 - Pf6N2E2_862 0.43 -0.1 1,010,972 + Pf6N2E2_862 0.44 -0.1
Or see this region's nucleotide sequence