Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_63 and Pf6N2E2_64 are separated by 11 nucleotides Pf6N2E2_64 and Pf6N2E2_65 are separated by 138 nucleotides Pf6N2E2_65 and Pf6N2E2_66 are separated by 66 nucleotides
Pf6N2E2_63 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 103,010 to 104,428
_63
Pf6N2E2_64 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 104,440 to 105,084
_64
Pf6N2E2_65 - SAM-dependent methyltransferase YafE (UbiE paralog), at 105,223 to 105,987
_65
Pf6N2E2_66 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 106,054 to 107,136
_66
Position (kb)
104
105
106 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 103.748 kb on + strand, within Pf6N2E2_63 at 103.749 kb on - strand, within Pf6N2E2_63 at 103.749 kb on - strand, within Pf6N2E2_63 at 103.997 kb on + strand, within Pf6N2E2_63 at 103.998 kb on - strand, within Pf6N2E2_63 at 104.318 kb on - strand at 104.350 kb on + strand at 104.602 kb on + strand, within Pf6N2E2_64 at 104.603 kb on - strand, within Pf6N2E2_64 at 104.660 kb on - strand, within Pf6N2E2_64 at 104.774 kb on - strand, within Pf6N2E2_64 at 105.092 kb on + strand at 105.251 kb on + strand at 105.252 kb on - strand at 105.252 kb on - strand at 105.283 kb on + strand at 105.329 kb on + strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.436 kb on + strand, within Pf6N2E2_65 at 105.436 kb on + strand, within Pf6N2E2_65 at 105.437 kb on - strand, within Pf6N2E2_65 at 105.697 kb on + strand, within Pf6N2E2_65 at 105.698 kb on - strand, within Pf6N2E2_65 at 105.698 kb on - strand, within Pf6N2E2_65 at 105.801 kb on + strand, within Pf6N2E2_65 at 105.802 kb on - strand, within Pf6N2E2_65 at 105.802 kb on - strand, within Pf6N2E2_65 at 105.829 kb on + strand, within Pf6N2E2_65 at 105.829 kb on + strand, within Pf6N2E2_65 at 105.855 kb on + strand, within Pf6N2E2_65 at 105.954 kb on + strand at 105.955 kb on - strand at 106.013 kb on - strand at 106.013 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 103,748 + Pf6N2E2_63 0.52 -0.1 103,749 - Pf6N2E2_63 0.52 -1.8 103,749 - Pf6N2E2_63 0.52 -1.0 103,997 + Pf6N2E2_63 0.70 +0.4 103,998 - Pf6N2E2_63 0.70 +0.7 104,318 - -0.4 104,350 + +0.6 104,602 + Pf6N2E2_64 0.25 -0.3 104,603 - Pf6N2E2_64 0.25 -1.0 104,660 - Pf6N2E2_64 0.34 -1.1 104,774 - Pf6N2E2_64 0.52 -0.6 105,092 + +1.1 105,251 + -0.9 105,252 - -0.2 105,252 - -0.4 105,283 + +0.9 105,329 + Pf6N2E2_65 0.14 +1.3 105,330 - Pf6N2E2_65 0.14 -0.6 105,330 - Pf6N2E2_65 0.14 -0.1 105,330 - Pf6N2E2_65 0.14 +1.1 105,330 - Pf6N2E2_65 0.14 -0.2 105,330 - Pf6N2E2_65 0.14 +2.3 105,436 + Pf6N2E2_65 0.28 -0.4 105,436 + Pf6N2E2_65 0.28 -0.2 105,437 - Pf6N2E2_65 0.28 -1.1 105,697 + Pf6N2E2_65 0.62 -0.4 105,698 - Pf6N2E2_65 0.62 -0.6 105,698 - Pf6N2E2_65 0.62 -0.1 105,801 + Pf6N2E2_65 0.76 -0.3 105,802 - Pf6N2E2_65 0.76 +0.9 105,802 - Pf6N2E2_65 0.76 -0.7 105,829 + Pf6N2E2_65 0.79 +0.3 105,829 + Pf6N2E2_65 0.79 -0.6 105,855 + Pf6N2E2_65 0.83 +0.2 105,954 + +0.2 105,955 - +0.8 106,013 - +0.4 106,013 - -0.7
Or see this region's nucleotide sequence