Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_64

Experiment: Nalidixic 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_63 and Pf6N2E2_64 are separated by 11 nucleotidesPf6N2E2_64 and Pf6N2E2_65 are separated by 138 nucleotidesPf6N2E2_65 and Pf6N2E2_66 are separated by 66 nucleotides Pf6N2E2_63 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 103,010 to 104,428 _63 Pf6N2E2_64 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 104,440 to 105,084 _64 Pf6N2E2_65 - SAM-dependent methyltransferase YafE (UbiE paralog), at 105,223 to 105,987 _65 Pf6N2E2_66 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 106,054 to 107,136 _66 Position (kb) 104 105 106Strain fitness (log2 ratio) -2 -1 0 1 2at 103.748 kb on + strand, within Pf6N2E2_63at 103.749 kb on - strand, within Pf6N2E2_63at 103.749 kb on - strand, within Pf6N2E2_63at 103.997 kb on + strand, within Pf6N2E2_63at 103.998 kb on - strand, within Pf6N2E2_63at 104.318 kb on - strandat 104.350 kb on + strandat 104.602 kb on + strand, within Pf6N2E2_64at 104.603 kb on - strand, within Pf6N2E2_64at 104.660 kb on - strand, within Pf6N2E2_64at 104.774 kb on - strand, within Pf6N2E2_64at 105.092 kb on + strandat 105.251 kb on + strandat 105.252 kb on - strandat 105.252 kb on - strandat 105.283 kb on + strandat 105.329 kb on + strand, within Pf6N2E2_65at 105.330 kb on - strand, within Pf6N2E2_65at 105.330 kb on - strand, within Pf6N2E2_65at 105.330 kb on - strand, within Pf6N2E2_65at 105.330 kb on - strand, within Pf6N2E2_65at 105.330 kb on - strand, within Pf6N2E2_65at 105.436 kb on + strand, within Pf6N2E2_65at 105.436 kb on + strand, within Pf6N2E2_65at 105.437 kb on - strand, within Pf6N2E2_65at 105.697 kb on + strand, within Pf6N2E2_65at 105.698 kb on - strand, within Pf6N2E2_65at 105.698 kb on - strand, within Pf6N2E2_65at 105.801 kb on + strand, within Pf6N2E2_65at 105.802 kb on - strand, within Pf6N2E2_65at 105.802 kb on - strand, within Pf6N2E2_65at 105.829 kb on + strand, within Pf6N2E2_65at 105.829 kb on + strand, within Pf6N2E2_65at 105.855 kb on + strand, within Pf6N2E2_65at 105.954 kb on + strandat 105.955 kb on - strandat 106.013 kb on - strandat 106.013 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml
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103,748 + Pf6N2E2_63 0.52 -0.1
103,749 - Pf6N2E2_63 0.52 -1.8
103,749 - Pf6N2E2_63 0.52 -1.0
103,997 + Pf6N2E2_63 0.70 +0.4
103,998 - Pf6N2E2_63 0.70 +0.7
104,318 - -0.4
104,350 + +0.6
104,602 + Pf6N2E2_64 0.25 -0.3
104,603 - Pf6N2E2_64 0.25 -1.0
104,660 - Pf6N2E2_64 0.34 -1.1
104,774 - Pf6N2E2_64 0.52 -0.6
105,092 + +1.1
105,251 + -0.9
105,252 - -0.2
105,252 - -0.4
105,283 + +0.9
105,329 + Pf6N2E2_65 0.14 +1.3
105,330 - Pf6N2E2_65 0.14 -0.6
105,330 - Pf6N2E2_65 0.14 -0.1
105,330 - Pf6N2E2_65 0.14 +1.1
105,330 - Pf6N2E2_65 0.14 -0.2
105,330 - Pf6N2E2_65 0.14 +2.3
105,436 + Pf6N2E2_65 0.28 -0.4
105,436 + Pf6N2E2_65 0.28 -0.2
105,437 - Pf6N2E2_65 0.28 -1.1
105,697 + Pf6N2E2_65 0.62 -0.4
105,698 - Pf6N2E2_65 0.62 -0.6
105,698 - Pf6N2E2_65 0.62 -0.1
105,801 + Pf6N2E2_65 0.76 -0.3
105,802 - Pf6N2E2_65 0.76 +0.9
105,802 - Pf6N2E2_65 0.76 -0.7
105,829 + Pf6N2E2_65 0.79 +0.3
105,829 + Pf6N2E2_65 0.79 -0.6
105,855 + Pf6N2E2_65 0.83 +0.2
105,954 + +0.2
105,955 - +0.8
106,013 - +0.4
106,013 - -0.7

Or see this region's nucleotide sequence