Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6066 and Pf6N2E2_6067 overlap by 1 nucleotides Pf6N2E2_6067 and Pf6N2E2_6068 overlap by 4 nucleotides Pf6N2E2_6068 and Pf6N2E2_6069 are separated by 35 nucleotides Pf6N2E2_6069 and Pf6N2E2_6070 overlap by 4 nucleotides Pf6N2E2_6070 and Pf6N2E2_6071 overlap by 4 nucleotides
Pf6N2E2_6066 - ABC transporter in pyoverdin gene cluster, permease component, at 6,888,150 to 6,889,049
_6066
Pf6N2E2_6067 - ABC transporter in pyoverdin gene cluster, ATP-binding component, at 6,889,049 to 6,889,801
_6067
Pf6N2E2_6068 - FIG014801: Cation ABC transporter, periplasmic cation-binding protein, at 6,889,798 to 6,890,727
_6068
Pf6N2E2_6069 - FIG049111: Hypothetical protein in pyoverdin gene cluster, at 6,890,763 to 6,891,338
_6069
Pf6N2E2_6070 - FIG137877: Hypothetical protein in pyoverdin gene cluster, at 6,891,335 to 6,891,661
_6070
Pf6N2E2_6071 - FIG139991: Putative thiamine pyrophosphate-requiring enzyme, at 6,891,658 to 6,892,200
_6071
Position (kb)
6889
6890
6891 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6888.815 kb on - strand, within Pf6N2E2_6066 at 6888.904 kb on - strand, within Pf6N2E2_6066 at 6888.904 kb on - strand, within Pf6N2E2_6066 at 6889.042 kb on + strand at 6889.042 kb on + strand at 6889.042 kb on + strand at 6889.042 kb on + strand at 6889.043 kb on - strand at 6889.043 kb on - strand at 6889.043 kb on - strand at 6889.043 kb on - strand at 6889.043 kb on - strand at 6889.260 kb on - strand, within Pf6N2E2_6067 at 6889.260 kb on - strand, within Pf6N2E2_6067 at 6889.260 kb on - strand, within Pf6N2E2_6067 at 6889.443 kb on + strand, within Pf6N2E2_6067 at 6889.443 kb on + strand, within Pf6N2E2_6067 at 6889.444 kb on - strand, within Pf6N2E2_6067 at 6889.444 kb on - strand, within Pf6N2E2_6067 at 6889.551 kb on + strand, within Pf6N2E2_6067 at 6889.877 kb on + strand at 6889.878 kb on - strand at 6889.893 kb on + strand, within Pf6N2E2_6068 at 6889.893 kb on + strand, within Pf6N2E2_6068 at 6889.893 kb on + strand, within Pf6N2E2_6068 at 6889.893 kb on + strand, within Pf6N2E2_6068 at 6889.894 kb on - strand, within Pf6N2E2_6068 at 6889.894 kb on - strand, within Pf6N2E2_6068 at 6889.946 kb on + strand, within Pf6N2E2_6068 at 6889.946 kb on + strand, within Pf6N2E2_6068 at 6889.946 kb on + strand, within Pf6N2E2_6068 at 6889.946 kb on + strand, within Pf6N2E2_6068 at 6889.946 kb on + strand, within Pf6N2E2_6068 at 6889.947 kb on - strand, within Pf6N2E2_6068 at 6889.947 kb on - strand, within Pf6N2E2_6068 at 6889.947 kb on - strand, within Pf6N2E2_6068 at 6889.947 kb on - strand, within Pf6N2E2_6068 at 6890.222 kb on + strand, within Pf6N2E2_6068 at 6890.223 kb on - strand, within Pf6N2E2_6068 at 6890.223 kb on - strand, within Pf6N2E2_6068 at 6890.271 kb on + strand, within Pf6N2E2_6068 at 6890.272 kb on - strand, within Pf6N2E2_6068 at 6890.310 kb on + strand, within Pf6N2E2_6068 at 6890.310 kb on + strand, within Pf6N2E2_6068 at 6890.310 kb on + strand, within Pf6N2E2_6068 at 6890.310 kb on + strand, within Pf6N2E2_6068 at 6890.310 kb on + strand, within Pf6N2E2_6068 at 6890.392 kb on + strand, within Pf6N2E2_6068 at 6890.516 kb on + strand, within Pf6N2E2_6068 at 6890.517 kb on - strand, within Pf6N2E2_6068 at 6890.588 kb on + strand, within Pf6N2E2_6068 at 6890.588 kb on + strand, within Pf6N2E2_6068 at 6890.588 kb on + strand, within Pf6N2E2_6068 at 6890.588 kb on + strand, within Pf6N2E2_6068 at 6890.588 kb on + strand, within Pf6N2E2_6068 at 6890.588 kb on + strand, within Pf6N2E2_6068 at 6890.588 kb on + strand, within Pf6N2E2_6068 at 6890.588 kb on + strand, within Pf6N2E2_6068 at 6890.588 kb on + strand, within Pf6N2E2_6068 at 6890.588 kb on + strand, within Pf6N2E2_6068 at 6890.588 kb on + strand, within Pf6N2E2_6068 at 6890.588 kb on + strand, within Pf6N2E2_6068 at 6890.588 kb on + strand, within Pf6N2E2_6068 at 6890.589 kb on - strand, within Pf6N2E2_6068 at 6890.589 kb on - strand, within Pf6N2E2_6068 at 6890.589 kb on - strand, within Pf6N2E2_6068 at 6890.589 kb on - strand, within Pf6N2E2_6068 at 6890.589 kb on - strand, within Pf6N2E2_6068 at 6890.589 kb on - strand, within Pf6N2E2_6068 at 6890.589 kb on - strand, within Pf6N2E2_6068 at 6890.594 kb on + strand, within Pf6N2E2_6068 at 6890.594 kb on + strand, within Pf6N2E2_6068 at 6890.594 kb on + strand, within Pf6N2E2_6068 at 6890.594 kb on + strand, within Pf6N2E2_6068 at 6890.595 kb on - strand, within Pf6N2E2_6068 at 6890.595 kb on - strand, within Pf6N2E2_6068 at 6890.595 kb on - strand, within Pf6N2E2_6068 at 6890.595 kb on - strand, within Pf6N2E2_6068 at 6890.595 kb on - strand, within Pf6N2E2_6068 at 6890.598 kb on - strand, within Pf6N2E2_6068 at 6890.598 kb on - strand, within Pf6N2E2_6068 at 6890.721 kb on + strand at 6890.721 kb on + strand at 6890.721 kb on + strand at 6890.722 kb on - strand at 6890.972 kb on - strand, within Pf6N2E2_6069 at 6890.972 kb on - strand, within Pf6N2E2_6069 at 6891.139 kb on + strand, within Pf6N2E2_6069 at 6891.139 kb on + strand, within Pf6N2E2_6069 at 6891.140 kb on - strand, within Pf6N2E2_6069 at 6891.280 kb on + strand, within Pf6N2E2_6069 at 6891.280 kb on + strand, within Pf6N2E2_6069 at 6891.280 kb on + strand, within Pf6N2E2_6069 at 6891.280 kb on + strand, within Pf6N2E2_6069 at 6891.280 kb on + strand, within Pf6N2E2_6069 at 6891.281 kb on - strand at 6891.281 kb on - strand at 6891.402 kb on + strand, within Pf6N2E2_6070 at 6891.402 kb on + strand, within Pf6N2E2_6070 at 6891.403 kb on - strand, within Pf6N2E2_6070 at 6891.403 kb on - strand, within Pf6N2E2_6070 at 6891.403 kb on - strand, within Pf6N2E2_6070
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 6,888,815 - Pf6N2E2_6066 0.74 -1.0 6,888,904 - Pf6N2E2_6066 0.84 -0.6 6,888,904 - Pf6N2E2_6066 0.84 -0.1 6,889,042 + +1.2 6,889,042 + -0.8 6,889,042 + -0.2 6,889,042 + -0.0 6,889,043 - +1.0 6,889,043 - +0.5 6,889,043 - -1.0 6,889,043 - +0.4 6,889,043 - +0.4 6,889,260 - Pf6N2E2_6067 0.28 +0.3 6,889,260 - Pf6N2E2_6067 0.28 +1.1 6,889,260 - Pf6N2E2_6067 0.28 -1.1 6,889,443 + Pf6N2E2_6067 0.52 -0.8 6,889,443 + Pf6N2E2_6067 0.52 +0.4 6,889,444 - Pf6N2E2_6067 0.52 -1.2 6,889,444 - Pf6N2E2_6067 0.52 +0.0 6,889,551 + Pf6N2E2_6067 0.67 +1.9 6,889,877 + -1.0 6,889,878 - -0.7 6,889,893 + Pf6N2E2_6068 0.10 -1.1 6,889,893 + Pf6N2E2_6068 0.10 -0.2 6,889,893 + Pf6N2E2_6068 0.10 -0.6 6,889,893 + Pf6N2E2_6068 0.10 -0.8 6,889,894 - Pf6N2E2_6068 0.10 -0.9 6,889,894 - Pf6N2E2_6068 0.10 -0.9 6,889,946 + Pf6N2E2_6068 0.16 -0.1 6,889,946 + Pf6N2E2_6068 0.16 +0.0 6,889,946 + Pf6N2E2_6068 0.16 -0.2 6,889,946 + Pf6N2E2_6068 0.16 +0.7 6,889,946 + Pf6N2E2_6068 0.16 +0.1 6,889,947 - Pf6N2E2_6068 0.16 -1.9 6,889,947 - Pf6N2E2_6068 0.16 -0.1 6,889,947 - Pf6N2E2_6068 0.16 -1.5 6,889,947 - Pf6N2E2_6068 0.16 -0.3 6,890,222 + Pf6N2E2_6068 0.46 -0.0 6,890,223 - Pf6N2E2_6068 0.46 -0.3 6,890,223 - Pf6N2E2_6068 0.46 -0.1 6,890,271 + Pf6N2E2_6068 0.51 -0.2 6,890,272 - Pf6N2E2_6068 0.51 +0.9 6,890,310 + Pf6N2E2_6068 0.55 -0.8 6,890,310 + Pf6N2E2_6068 0.55 +1.6 6,890,310 + Pf6N2E2_6068 0.55 -1.9 6,890,310 + Pf6N2E2_6068 0.55 -0.3 6,890,310 + Pf6N2E2_6068 0.55 -0.6 6,890,392 + Pf6N2E2_6068 0.64 +0.9 6,890,516 + Pf6N2E2_6068 0.77 +0.4 6,890,517 - Pf6N2E2_6068 0.77 -0.2 6,890,588 + Pf6N2E2_6068 0.85 -0.7 6,890,588 + Pf6N2E2_6068 0.85 +0.0 6,890,588 + Pf6N2E2_6068 0.85 -1.5 6,890,588 + Pf6N2E2_6068 0.85 -1.9 6,890,588 + Pf6N2E2_6068 0.85 -1.1 6,890,588 + Pf6N2E2_6068 0.85 +0.1 6,890,588 + Pf6N2E2_6068 0.85 +0.4 6,890,588 + Pf6N2E2_6068 0.85 -1.8 6,890,588 + Pf6N2E2_6068 0.85 -0.8 6,890,588 + Pf6N2E2_6068 0.85 -0.3 6,890,588 + Pf6N2E2_6068 0.85 +1.2 6,890,588 + Pf6N2E2_6068 0.85 -0.9 6,890,588 + Pf6N2E2_6068 0.85 +1.2 6,890,589 - Pf6N2E2_6068 0.85 -1.5 6,890,589 - Pf6N2E2_6068 0.85 +1.2 6,890,589 - Pf6N2E2_6068 0.85 -0.2 6,890,589 - Pf6N2E2_6068 0.85 +0.0 6,890,589 - Pf6N2E2_6068 0.85 -0.9 6,890,589 - Pf6N2E2_6068 0.85 +0.2 6,890,589 - Pf6N2E2_6068 0.85 -0.9 6,890,594 + Pf6N2E2_6068 0.86 -1.0 6,890,594 + Pf6N2E2_6068 0.86 -0.5 6,890,594 + Pf6N2E2_6068 0.86 -1.8 6,890,594 + Pf6N2E2_6068 0.86 -0.0 6,890,595 - Pf6N2E2_6068 0.86 -0.7 6,890,595 - Pf6N2E2_6068 0.86 -1.1 6,890,595 - Pf6N2E2_6068 0.86 -1.5 6,890,595 - Pf6N2E2_6068 0.86 -2.2 6,890,595 - Pf6N2E2_6068 0.86 -1.0 6,890,598 - Pf6N2E2_6068 0.86 +0.0 6,890,598 - Pf6N2E2_6068 0.86 -0.6 6,890,721 + -0.2 6,890,721 + +0.1 6,890,721 + +1.3 6,890,722 - -0.9 6,890,972 - Pf6N2E2_6069 0.36 -0.7 6,890,972 - Pf6N2E2_6069 0.36 -0.0 6,891,139 + Pf6N2E2_6069 0.65 -1.4 6,891,139 + Pf6N2E2_6069 0.65 -2.4 6,891,140 - Pf6N2E2_6069 0.65 +0.5 6,891,280 + Pf6N2E2_6069 0.90 -3.1 6,891,280 + Pf6N2E2_6069 0.90 +0.4 6,891,280 + Pf6N2E2_6069 0.90 -0.0 6,891,280 + Pf6N2E2_6069 0.90 +0.2 6,891,280 + Pf6N2E2_6069 0.90 -0.4 6,891,281 - -0.4 6,891,281 - +0.5 6,891,402 + Pf6N2E2_6070 0.20 -0.6 6,891,402 + Pf6N2E2_6070 0.20 +0.4 6,891,403 - Pf6N2E2_6070 0.21 -0.4 6,891,403 - Pf6N2E2_6070 0.21 -0.2 6,891,403 - Pf6N2E2_6070 0.21 +0.1
Or see this region's nucleotide sequence