Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5621 and Pf6N2E2_5622 are separated by 7 nucleotides Pf6N2E2_5622 and Pf6N2E2_5623 are separated by 107 nucleotides Pf6N2E2_5623 and Pf6N2E2_5624 are separated by 1 nucleotides Pf6N2E2_5624 and Pf6N2E2_5625 are separated by 87 nucleotides
Pf6N2E2_5621 - DNA-binding response regulator, at 6,438,595 to 6,439,272
_5621
Pf6N2E2_5622 - Translation initiation factor 2 (IF-2; GTPase), at 6,439,280 to 6,439,690
_5622
Pf6N2E2_5623 - YciL protein, at 6,439,798 to 6,440,097
_5623
Pf6N2E2_5624 - Intracellular septation protein IspA, at 6,440,099 to 6,440,695
_5624
Pf6N2E2_5625 - COG0613, Predicted metal-dependent phosphoesterases (PHP family), at 6,440,783 to 6,441,646
_5625
Position (kb)
6439
6440
6441 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 6438.923 kb on - strand, within Pf6N2E2_5621 at 6438.977 kb on + strand, within Pf6N2E2_5621 at 6438.978 kb on - strand, within Pf6N2E2_5621 at 6439.124 kb on - strand, within Pf6N2E2_5621 at 6439.253 kb on - strand at 6439.253 kb on - strand at 6439.259 kb on - strand at 6439.259 kb on - strand at 6439.306 kb on + strand at 6439.306 kb on + strand at 6439.306 kb on + strand at 6439.306 kb on + strand at 6439.306 kb on + strand at 6439.327 kb on + strand, within Pf6N2E2_5622 at 6439.327 kb on + strand, within Pf6N2E2_5622 at 6439.347 kb on - strand, within Pf6N2E2_5622 at 6439.665 kb on - strand at 6439.665 kb on - strand at 6439.717 kb on + strand at 6439.718 kb on - strand at 6439.718 kb on - strand at 6439.718 kb on - strand at 6439.800 kb on - strand at 6439.841 kb on + strand, within Pf6N2E2_5623 at 6439.841 kb on + strand, within Pf6N2E2_5623 at 6439.842 kb on - strand, within Pf6N2E2_5623 at 6439.987 kb on + strand, within Pf6N2E2_5623 at 6440.090 kb on + strand at 6440.090 kb on + strand at 6440.120 kb on - strand at 6440.727 kb on - strand at 6440.729 kb on + strand at 6440.729 kb on + strand at 6440.730 kb on - strand at 6440.730 kb on - strand at 6440.772 kb on - strand at 6440.806 kb on + strand at 6440.806 kb on + strand at 6440.807 kb on - strand at 6440.980 kb on + strand, within Pf6N2E2_5625
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 6,438,923 - Pf6N2E2_5621 0.48 +0.3 6,438,977 + Pf6N2E2_5621 0.56 -0.3 6,438,978 - Pf6N2E2_5621 0.56 -0.6 6,439,124 - Pf6N2E2_5621 0.78 +0.2 6,439,253 - +0.6 6,439,253 - +0.3 6,439,259 - +0.3 6,439,259 - +1.4 6,439,306 + -0.6 6,439,306 + +0.3 6,439,306 + +0.0 6,439,306 + +0.6 6,439,306 + -0.2 6,439,327 + Pf6N2E2_5622 0.11 -0.0 6,439,327 + Pf6N2E2_5622 0.11 -0.6 6,439,347 - Pf6N2E2_5622 0.16 +0.2 6,439,665 - -0.1 6,439,665 - -0.9 6,439,717 + -0.1 6,439,718 - +0.3 6,439,718 - +1.1 6,439,718 - -0.6 6,439,800 - -0.4 6,439,841 + Pf6N2E2_5623 0.14 -0.2 6,439,841 + Pf6N2E2_5623 0.14 -0.3 6,439,842 - Pf6N2E2_5623 0.15 -0.9 6,439,987 + Pf6N2E2_5623 0.63 -1.6 6,440,090 + -0.0 6,440,090 + -1.0 6,440,120 - -4.5 6,440,727 - -1.8 6,440,729 + -1.3 6,440,729 + -1.4 6,440,730 - +0.7 6,440,730 - -0.7 6,440,772 - -0.7 6,440,806 + -2.7 6,440,806 + -3.5 6,440,807 - -0.2 6,440,980 + Pf6N2E2_5625 0.23 -0.1
Or see this region's nucleotide sequence