Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_545 and Pf6N2E2_546 are separated by 198 nucleotides Pf6N2E2_546 and Pf6N2E2_547 are separated by 221 nucleotides Pf6N2E2_547 and Pf6N2E2_548 are separated by 13 nucleotides
Pf6N2E2_545 - Predicted aminoglycoside phosphotransferase, at 614,275 to 615,342
_545
Pf6N2E2_546 - FIG00966904: hypothetical protein, at 615,541 to 615,936
_546
Pf6N2E2_547 - Phophatidylinositol-4-phosphate 5-kinase (EC 2.7.1.68), at 616,158 to 616,700
_547
Pf6N2E2_548 - VgrG protein, at 616,714 to 618,723
_548
Position (kb)
616
617 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 615.194 kb on + strand, within Pf6N2E2_545 at 615.194 kb on + strand, within Pf6N2E2_545 at 615.195 kb on - strand, within Pf6N2E2_545 at 615.195 kb on - strand, within Pf6N2E2_545 at 615.272 kb on + strand at 615.360 kb on - strand at 615.429 kb on + strand at 615.429 kb on + strand at 615.430 kb on - strand at 615.430 kb on - strand at 615.430 kb on - strand at 615.430 kb on - strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.501 kb on + strand at 615.859 kb on - strand, within Pf6N2E2_546 at 615.876 kb on + strand, within Pf6N2E2_546 at 615.877 kb on - strand, within Pf6N2E2_546 at 615.938 kb on + strand at 615.963 kb on + strand at 615.963 kb on + strand at 615.963 kb on + strand at 615.963 kb on + strand at 615.964 kb on - strand at 615.964 kb on - strand at 615.964 kb on - strand at 615.964 kb on - strand at 615.967 kb on + strand at 615.967 kb on + strand at 615.967 kb on + strand at 615.967 kb on + strand at 615.967 kb on + strand at 615.967 kb on + strand at 615.967 kb on + strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.972 kb on + strand at 615.973 kb on - strand at 615.973 kb on - strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.977 kb on - strand at 615.977 kb on - strand at 615.977 kb on - strand at 615.977 kb on - strand at 615.977 kb on - strand at 615.984 kb on + strand at 615.984 kb on + strand at 615.984 kb on + strand at 615.984 kb on + strand at 615.985 kb on - strand at 615.985 kb on - strand at 615.985 kb on - strand at 616.092 kb on + strand at 616.160 kb on - strand at 616.160 kb on - strand at 616.160 kb on - strand at 616.160 kb on - strand at 616.396 kb on + strand, within Pf6N2E2_547 at 616.397 kb on - strand, within Pf6N2E2_547 at 616.397 kb on - strand, within Pf6N2E2_547 at 616.433 kb on - strand, within Pf6N2E2_547 at 616.469 kb on - strand, within Pf6N2E2_547 at 616.469 kb on - strand, within Pf6N2E2_547 at 616.576 kb on + strand, within Pf6N2E2_547 at 617.012 kb on + strand, within Pf6N2E2_548 at 617.036 kb on + strand, within Pf6N2E2_548 at 617.418 kb on - strand, within Pf6N2E2_548 at 617.612 kb on + strand, within Pf6N2E2_548 at 617.613 kb on - strand, within Pf6N2E2_548 at 617.669 kb on + strand, within Pf6N2E2_548 at 617.669 kb on + strand, within Pf6N2E2_548 at 617.670 kb on - strand, within Pf6N2E2_548 at 617.670 kb on - strand, within Pf6N2E2_548 at 617.670 kb on - strand, within Pf6N2E2_548
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 615,194 + Pf6N2E2_545 0.86 -2.9 615,194 + Pf6N2E2_545 0.86 +0.9 615,195 - Pf6N2E2_545 0.86 +0.1 615,195 - Pf6N2E2_545 0.86 +1.5 615,272 + -0.7 615,360 - +0.7 615,429 + +0.1 615,429 + +0.1 615,430 - +1.0 615,430 - -1.7 615,430 - -0.4 615,430 - -0.3 615,431 + -0.1 615,431 + -0.7 615,431 + -1.7 615,431 + -1.2 615,431 + -1.1 615,431 + -0.1 615,431 + +0.4 615,431 + -1.2 615,431 + -0.7 615,431 + -0.7 615,431 + -1.3 615,432 - +0.9 615,432 - +0.3 615,432 - -0.3 615,432 - -1.7 615,432 - -0.7 615,432 - +0.2 615,432 - -0.8 615,432 - -0.5 615,501 + +1.1 615,859 - Pf6N2E2_546 0.80 +0.4 615,876 + Pf6N2E2_546 0.85 -2.2 615,877 - Pf6N2E2_546 0.85 +0.1 615,938 + +0.0 615,963 + -1.7 615,963 + -0.2 615,963 + +0.9 615,963 + -0.2 615,964 - +1.6 615,964 - +1.1 615,964 - +0.8 615,964 - +0.7 615,967 + -0.2 615,967 + -0.5 615,967 + -0.8 615,967 + +0.8 615,967 + -0.5 615,967 + -0.4 615,967 + +0.8 615,968 - +1.0 615,968 - -0.4 615,968 - -0.7 615,968 - +0.6 615,968 - -0.9 615,968 - -2.2 615,968 - +0.5 615,968 - +0.7 615,968 - -0.7 615,968 - -0.3 615,968 - +0.1 615,968 - -0.1 615,968 - +1.1 615,972 + +0.6 615,973 - -1.4 615,973 - +0.1 615,976 + -0.9 615,976 + +0.1 615,976 + -1.2 615,976 + +0.3 615,976 + +0.4 615,976 + +0.5 615,976 + -0.0 615,976 + -0.1 615,976 + -1.0 615,976 + -2.0 615,977 - -0.1 615,977 - -0.2 615,977 - -1.3 615,977 - -1.3 615,977 - +0.8 615,984 + +0.4 615,984 + +0.4 615,984 + +0.1 615,984 + +0.4 615,985 - -2.1 615,985 - +0.2 615,985 - -1.4 616,092 + +0.6 616,160 - +0.4 616,160 - -0.8 616,160 - +0.4 616,160 - -0.9 616,396 + Pf6N2E2_547 0.44 -0.5 616,397 - Pf6N2E2_547 0.44 -0.1 616,397 - Pf6N2E2_547 0.44 -1.4 616,433 - Pf6N2E2_547 0.51 -2.2 616,469 - Pf6N2E2_547 0.57 -0.3 616,469 - Pf6N2E2_547 0.57 -0.8 616,576 + Pf6N2E2_547 0.77 -1.6 617,012 + Pf6N2E2_548 0.15 -0.8 617,036 + Pf6N2E2_548 0.16 +0.2 617,418 - Pf6N2E2_548 0.35 -1.0 617,612 + Pf6N2E2_548 0.45 -0.4 617,613 - Pf6N2E2_548 0.45 -0.4 617,669 + Pf6N2E2_548 0.48 +0.9 617,669 + Pf6N2E2_548 0.48 +0.1 617,670 - Pf6N2E2_548 0.48 +1.6 617,670 - Pf6N2E2_548 0.48 -1.7 617,670 - Pf6N2E2_548 0.48 +1.1
Or see this region's nucleotide sequence