Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5365 and Pf6N2E2_5366 overlap by 14 nucleotides Pf6N2E2_5366 and Pf6N2E2_5367 are separated by 9 nucleotides Pf6N2E2_5367 and Pf6N2E2_5368 overlap by 4 nucleotides
Pf6N2E2_5365 - Heavy metal sensor histidine kinase, at 6,163,226 to 6,164,593
_5365
Pf6N2E2_5366 - DNA-binding heavy metal response regulator, at 6,164,580 to 6,165,263
_5366
Pf6N2E2_5367 - Cobalt-zinc-cadmium resistance protein CzcA; Cation efflux system protein CusA, at 6,165,273 to 6,168,389
_5367
Pf6N2E2_5368 - Probable Co/Zn/Cd efflux system membrane fusion protein, at 6,168,386 to 6,169,543
_5368
Position (kb)
6165
6166
6167
6168
6169 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6164.446 kb on - strand, within Pf6N2E2_5365 at 6164.446 kb on - strand, within Pf6N2E2_5365 at 6164.502 kb on + strand at 6164.503 kb on - strand at 6164.503 kb on - strand at 6164.505 kb on - strand at 6164.505 kb on - strand at 6164.505 kb on - strand at 6164.505 kb on - strand at 6164.614 kb on + strand at 6164.615 kb on - strand at 6164.615 kb on - strand at 6164.615 kb on - strand at 6164.974 kb on + strand, within Pf6N2E2_5366 at 6164.974 kb on + strand, within Pf6N2E2_5366 at 6164.975 kb on - strand, within Pf6N2E2_5366 at 6164.975 kb on - strand, within Pf6N2E2_5366 at 6164.975 kb on - strand, within Pf6N2E2_5366 at 6164.975 kb on - strand, within Pf6N2E2_5366 at 6165.078 kb on + strand, within Pf6N2E2_5366 at 6165.079 kb on - strand, within Pf6N2E2_5366 at 6165.131 kb on - strand, within Pf6N2E2_5366 at 6165.189 kb on - strand, within Pf6N2E2_5366 at 6165.190 kb on + strand, within Pf6N2E2_5366 at 6165.190 kb on + strand, within Pf6N2E2_5366 at 6165.190 kb on + strand, within Pf6N2E2_5366 at 6165.220 kb on + strand at 6165.221 kb on - strand at 6165.221 kb on - strand at 6165.358 kb on + strand at 6165.358 kb on + strand at 6165.358 kb on + strand at 6165.358 kb on + strand at 6165.358 kb on + strand at 6165.358 kb on + strand at 6165.358 kb on + strand at 6165.358 kb on + strand at 6165.358 kb on + strand at 6165.358 kb on + strand at 6165.358 kb on + strand at 6165.358 kb on + strand at 6165.358 kb on + strand at 6165.358 kb on + strand at 6165.358 kb on + strand at 6165.358 kb on + strand at 6165.358 kb on + strand at 6165.359 kb on - strand at 6165.359 kb on - strand at 6165.359 kb on - strand at 6165.359 kb on - strand at 6165.359 kb on - strand at 6165.359 kb on - strand at 6165.359 kb on - strand at 6165.359 kb on - strand at 6165.359 kb on - strand at 6165.359 kb on - strand at 6165.359 kb on - strand at 6165.359 kb on - strand at 6165.359 kb on - strand at 6165.359 kb on - strand at 6165.359 kb on - strand at 6165.359 kb on - strand at 6165.359 kb on - strand at 6165.359 kb on - strand at 6165.360 kb on + strand at 6165.360 kb on + strand at 6165.360 kb on + strand at 6165.361 kb on - strand at 6165.361 kb on - strand at 6165.760 kb on + strand, within Pf6N2E2_5367 at 6165.760 kb on + strand, within Pf6N2E2_5367 at 6165.781 kb on + strand, within Pf6N2E2_5367 at 6165.781 kb on + strand, within Pf6N2E2_5367 at 6165.781 kb on + strand, within Pf6N2E2_5367 at 6165.781 kb on + strand, within Pf6N2E2_5367 at 6165.781 kb on + strand, within Pf6N2E2_5367 at 6165.782 kb on - strand, within Pf6N2E2_5367 at 6165.782 kb on - strand, within Pf6N2E2_5367 at 6165.782 kb on - strand, within Pf6N2E2_5367 at 6165.782 kb on - strand, within Pf6N2E2_5367 at 6165.782 kb on - strand, within Pf6N2E2_5367 at 6165.782 kb on - strand, within Pf6N2E2_5367 at 6165.782 kb on - strand, within Pf6N2E2_5367 at 6166.079 kb on - strand, within Pf6N2E2_5367 at 6166.085 kb on - strand, within Pf6N2E2_5367 at 6166.141 kb on + strand, within Pf6N2E2_5367 at 6166.141 kb on + strand, within Pf6N2E2_5367 at 6166.142 kb on - strand, within Pf6N2E2_5367 at 6166.195 kb on + strand, within Pf6N2E2_5367 at 6166.195 kb on + strand, within Pf6N2E2_5367 at 6166.390 kb on + strand, within Pf6N2E2_5367 at 6166.390 kb on + strand, within Pf6N2E2_5367 at 6166.390 kb on + strand, within Pf6N2E2_5367 at 6166.390 kb on + strand, within Pf6N2E2_5367 at 6166.390 kb on + strand, within Pf6N2E2_5367 at 6166.390 kb on + strand, within Pf6N2E2_5367 at 6166.390 kb on + strand, within Pf6N2E2_5367 at 6166.390 kb on + strand, within Pf6N2E2_5367 at 6166.390 kb on + strand, within Pf6N2E2_5367 at 6166.391 kb on - strand, within Pf6N2E2_5367 at 6166.391 kb on - strand, within Pf6N2E2_5367 at 6166.391 kb on - strand, within Pf6N2E2_5367 at 6166.391 kb on - strand, within Pf6N2E2_5367 at 6166.391 kb on - strand, within Pf6N2E2_5367 at 6166.391 kb on - strand, within Pf6N2E2_5367 at 6166.391 kb on - strand, within Pf6N2E2_5367 at 6166.450 kb on + strand, within Pf6N2E2_5367 at 6166.450 kb on + strand, within Pf6N2E2_5367 at 6166.458 kb on + strand, within Pf6N2E2_5367 at 6166.458 kb on + strand, within Pf6N2E2_5367 at 6166.459 kb on - strand, within Pf6N2E2_5367 at 6166.459 kb on - strand, within Pf6N2E2_5367 at 6166.711 kb on - strand, within Pf6N2E2_5367 at 6166.711 kb on - strand, within Pf6N2E2_5367 at 6166.760 kb on - strand, within Pf6N2E2_5367 at 6166.760 kb on - strand, within Pf6N2E2_5367 at 6166.800 kb on + strand, within Pf6N2E2_5367 at 6166.801 kb on - strand, within Pf6N2E2_5367 at 6166.801 kb on - strand, within Pf6N2E2_5367 at 6166.801 kb on - strand, within Pf6N2E2_5367 at 6166.801 kb on - strand, within Pf6N2E2_5367 at 6166.801 kb on - strand, within Pf6N2E2_5367 at 6166.897 kb on - strand, within Pf6N2E2_5367 at 6167.245 kb on + strand, within Pf6N2E2_5367 at 6167.245 kb on + strand, within Pf6N2E2_5367 at 6167.245 kb on + strand, within Pf6N2E2_5367 at 6167.246 kb on - strand, within Pf6N2E2_5367 at 6167.246 kb on - strand, within Pf6N2E2_5367 at 6167.246 kb on - strand, within Pf6N2E2_5367 at 6167.246 kb on - strand, within Pf6N2E2_5367 at 6167.246 kb on - strand, within Pf6N2E2_5367 at 6167.246 kb on - strand, within Pf6N2E2_5367 at 6167.246 kb on - strand, within Pf6N2E2_5367 at 6167.246 kb on - strand, within Pf6N2E2_5367 at 6167.246 kb on - strand, within Pf6N2E2_5367 at 6167.400 kb on - strand, within Pf6N2E2_5367 at 6167.421 kb on + strand, within Pf6N2E2_5367 at 6167.422 kb on - strand, within Pf6N2E2_5367 at 6167.422 kb on - strand, within Pf6N2E2_5367 at 6167.422 kb on - strand, within Pf6N2E2_5367 at 6167.422 kb on - strand, within Pf6N2E2_5367 at 6167.422 kb on - strand, within Pf6N2E2_5367 at 6167.433 kb on + strand, within Pf6N2E2_5367 at 6167.433 kb on + strand, within Pf6N2E2_5367 at 6167.434 kb on - strand, within Pf6N2E2_5367 at 6167.635 kb on + strand, within Pf6N2E2_5367 at 6167.636 kb on - strand, within Pf6N2E2_5367 at 6167.656 kb on + strand, within Pf6N2E2_5367 at 6167.656 kb on + strand, within Pf6N2E2_5367 at 6167.657 kb on - strand, within Pf6N2E2_5367 at 6167.657 kb on - strand, within Pf6N2E2_5367 at 6167.717 kb on - strand, within Pf6N2E2_5367 at 6167.851 kb on + strand, within Pf6N2E2_5367 at 6167.851 kb on + strand, within Pf6N2E2_5367 at 6167.851 kb on + strand, within Pf6N2E2_5367 at 6167.851 kb on + strand, within Pf6N2E2_5367 at 6167.851 kb on + strand, within Pf6N2E2_5367 at 6167.851 kb on + strand, within Pf6N2E2_5367 at 6167.852 kb on - strand, within Pf6N2E2_5367 at 6167.852 kb on - strand, within Pf6N2E2_5367 at 6167.862 kb on + strand, within Pf6N2E2_5367 at 6167.862 kb on + strand, within Pf6N2E2_5367 at 6167.908 kb on + strand, within Pf6N2E2_5367 at 6167.908 kb on + strand, within Pf6N2E2_5367 at 6168.518 kb on + strand, within Pf6N2E2_5368 at 6168.518 kb on + strand, within Pf6N2E2_5368 at 6168.518 kb on + strand, within Pf6N2E2_5368 at 6168.518 kb on + strand, within Pf6N2E2_5368 at 6168.518 kb on + strand, within Pf6N2E2_5368 at 6168.518 kb on + strand, within Pf6N2E2_5368 at 6168.518 kb on + strand, within Pf6N2E2_5368 at 6168.518 kb on + strand, within Pf6N2E2_5368 at 6168.518 kb on + strand, within Pf6N2E2_5368 at 6168.518 kb on + strand, within Pf6N2E2_5368 at 6168.518 kb on + strand, within Pf6N2E2_5368 at 6168.518 kb on + strand, within Pf6N2E2_5368 at 6168.518 kb on + strand, within Pf6N2E2_5368 at 6168.518 kb on + strand, within Pf6N2E2_5368 at 6168.518 kb on + strand, within Pf6N2E2_5368 at 6168.519 kb on - strand, within Pf6N2E2_5368 at 6168.519 kb on - strand, within Pf6N2E2_5368 at 6168.519 kb on - strand, within Pf6N2E2_5368 at 6168.519 kb on - strand, within Pf6N2E2_5368 at 6168.519 kb on - strand, within Pf6N2E2_5368 at 6168.519 kb on - strand, within Pf6N2E2_5368 at 6168.519 kb on - strand, within Pf6N2E2_5368 at 6168.519 kb on - strand, within Pf6N2E2_5368 at 6168.519 kb on - strand, within Pf6N2E2_5368 at 6168.519 kb on - strand, within Pf6N2E2_5368 at 6168.519 kb on - strand, within Pf6N2E2_5368 at 6168.534 kb on + strand, within Pf6N2E2_5368 at 6168.819 kb on + strand, within Pf6N2E2_5368 at 6168.820 kb on - strand, within Pf6N2E2_5368 at 6168.820 kb on - strand, within Pf6N2E2_5368 at 6169.023 kb on + strand, within Pf6N2E2_5368 at 6169.113 kb on + strand, within Pf6N2E2_5368 at 6169.114 kb on - strand, within Pf6N2E2_5368 at 6169.114 kb on - strand, within Pf6N2E2_5368
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 6,164,446 - Pf6N2E2_5365 0.89 +0.4 6,164,446 - Pf6N2E2_5365 0.89 -0.1 6,164,502 + -0.1 6,164,503 - +1.2 6,164,503 - -3.0 6,164,505 - +0.4 6,164,505 - -3.3 6,164,505 - +0.1 6,164,505 - +0.1 6,164,614 + +0.9 6,164,615 - -0.0 6,164,615 - -0.5 6,164,615 - +0.5 6,164,974 + Pf6N2E2_5366 0.58 +0.4 6,164,974 + Pf6N2E2_5366 0.58 +0.2 6,164,975 - Pf6N2E2_5366 0.58 -0.8 6,164,975 - Pf6N2E2_5366 0.58 -0.2 6,164,975 - Pf6N2E2_5366 0.58 -1.8 6,164,975 - Pf6N2E2_5366 0.58 +0.1 6,165,078 + Pf6N2E2_5366 0.73 -0.7 6,165,079 - Pf6N2E2_5366 0.73 -0.1 6,165,131 - Pf6N2E2_5366 0.81 +0.1 6,165,189 - Pf6N2E2_5366 0.89 +1.4 6,165,190 + Pf6N2E2_5366 0.89 -0.7 6,165,190 + Pf6N2E2_5366 0.89 -0.2 6,165,190 + Pf6N2E2_5366 0.89 -0.8 6,165,220 + +1.2 6,165,221 - -1.4 6,165,221 - -0.7 6,165,358 + -0.5 6,165,358 + +0.8 6,165,358 + +0.2 6,165,358 + +0.1 6,165,358 + -1.5 6,165,358 + -0.2 6,165,358 + -0.1 6,165,358 + +0.6 6,165,358 + -0.6 6,165,358 + +0.0 6,165,358 + -0.6 6,165,358 + -0.5 6,165,358 + +0.7 6,165,358 + +0.2 6,165,358 + -2.6 6,165,358 + +0.6 6,165,358 + +0.8 6,165,359 - -0.6 6,165,359 - -1.0 6,165,359 - -0.5 6,165,359 - +1.7 6,165,359 - -0.9 6,165,359 - +0.3 6,165,359 - +1.2 6,165,359 - +0.1 6,165,359 - -1.2 6,165,359 - -0.5 6,165,359 - -0.6 6,165,359 - -0.6 6,165,359 - -1.5 6,165,359 - -0.3 6,165,359 - +0.6 6,165,359 - -0.6 6,165,359 - +0.1 6,165,359 - -1.0 6,165,360 + -1.9 6,165,360 + -0.9 6,165,360 + +0.2 6,165,361 - +1.2 6,165,361 - +0.5 6,165,760 + Pf6N2E2_5367 0.16 +1.1 6,165,760 + Pf6N2E2_5367 0.16 -1.5 6,165,781 + Pf6N2E2_5367 0.16 -0.3 6,165,781 + Pf6N2E2_5367 0.16 -0.3 6,165,781 + Pf6N2E2_5367 0.16 -0.5 6,165,781 + Pf6N2E2_5367 0.16 -2.1 6,165,781 + Pf6N2E2_5367 0.16 -0.6 6,165,782 - Pf6N2E2_5367 0.16 +0.4 6,165,782 - Pf6N2E2_5367 0.16 -0.7 6,165,782 - Pf6N2E2_5367 0.16 +0.7 6,165,782 - Pf6N2E2_5367 0.16 +0.6 6,165,782 - Pf6N2E2_5367 0.16 -0.4 6,165,782 - Pf6N2E2_5367 0.16 -0.4 6,165,782 - Pf6N2E2_5367 0.16 +0.2 6,166,079 - Pf6N2E2_5367 0.26 -0.9 6,166,085 - Pf6N2E2_5367 0.26 +0.2 6,166,141 + Pf6N2E2_5367 0.28 +0.0 6,166,141 + Pf6N2E2_5367 0.28 -0.9 6,166,142 - Pf6N2E2_5367 0.28 +0.5 6,166,195 + Pf6N2E2_5367 0.30 -1.8 6,166,195 + Pf6N2E2_5367 0.30 +0.4 6,166,390 + Pf6N2E2_5367 0.36 +0.3 6,166,390 + Pf6N2E2_5367 0.36 -0.7 6,166,390 + Pf6N2E2_5367 0.36 -0.8 6,166,390 + Pf6N2E2_5367 0.36 -0.0 6,166,390 + Pf6N2E2_5367 0.36 -0.2 6,166,390 + Pf6N2E2_5367 0.36 -0.5 6,166,390 + Pf6N2E2_5367 0.36 +0.2 6,166,390 + Pf6N2E2_5367 0.36 +0.4 6,166,390 + Pf6N2E2_5367 0.36 -0.4 6,166,391 - Pf6N2E2_5367 0.36 -0.6 6,166,391 - Pf6N2E2_5367 0.36 -1.1 6,166,391 - Pf6N2E2_5367 0.36 +1.1 6,166,391 - Pf6N2E2_5367 0.36 -1.1 6,166,391 - Pf6N2E2_5367 0.36 -0.1 6,166,391 - Pf6N2E2_5367 0.36 +0.2 6,166,391 - Pf6N2E2_5367 0.36 -0.3 6,166,450 + Pf6N2E2_5367 0.38 -0.1 6,166,450 + Pf6N2E2_5367 0.38 -0.3 6,166,458 + Pf6N2E2_5367 0.38 +1.4 6,166,458 + Pf6N2E2_5367 0.38 -2.6 6,166,459 - Pf6N2E2_5367 0.38 +1.1 6,166,459 - Pf6N2E2_5367 0.38 -0.3 6,166,711 - Pf6N2E2_5367 0.46 -0.9 6,166,711 - Pf6N2E2_5367 0.46 -2.2 6,166,760 - Pf6N2E2_5367 0.48 -1.4 6,166,760 - Pf6N2E2_5367 0.48 -0.8 6,166,800 + Pf6N2E2_5367 0.49 -1.3 6,166,801 - Pf6N2E2_5367 0.49 -0.7 6,166,801 - Pf6N2E2_5367 0.49 -0.6 6,166,801 - Pf6N2E2_5367 0.49 -0.6 6,166,801 - Pf6N2E2_5367 0.49 -0.5 6,166,801 - Pf6N2E2_5367 0.49 -0.4 6,166,897 - Pf6N2E2_5367 0.52 +1.0 6,167,245 + Pf6N2E2_5367 0.63 +0.4 6,167,245 + Pf6N2E2_5367 0.63 +0.5 6,167,245 + Pf6N2E2_5367 0.63 +0.0 6,167,246 - Pf6N2E2_5367 0.63 +1.0 6,167,246 - Pf6N2E2_5367 0.63 -0.1 6,167,246 - Pf6N2E2_5367 0.63 -0.0 6,167,246 - Pf6N2E2_5367 0.63 -0.5 6,167,246 - Pf6N2E2_5367 0.63 -1.8 6,167,246 - Pf6N2E2_5367 0.63 -0.6 6,167,246 - Pf6N2E2_5367 0.63 +0.3 6,167,246 - Pf6N2E2_5367 0.63 -1.3 6,167,246 - Pf6N2E2_5367 0.63 -1.4 6,167,400 - Pf6N2E2_5367 0.68 +1.7 6,167,421 + Pf6N2E2_5367 0.69 +0.8 6,167,422 - Pf6N2E2_5367 0.69 +0.1 6,167,422 - Pf6N2E2_5367 0.69 +0.4 6,167,422 - Pf6N2E2_5367 0.69 -0.1 6,167,422 - Pf6N2E2_5367 0.69 -1.2 6,167,422 - Pf6N2E2_5367 0.69 +0.3 6,167,433 + Pf6N2E2_5367 0.69 -0.6 6,167,433 + Pf6N2E2_5367 0.69 +0.7 6,167,434 - Pf6N2E2_5367 0.69 -0.6 6,167,635 + Pf6N2E2_5367 0.76 -0.3 6,167,636 - Pf6N2E2_5367 0.76 -0.4 6,167,656 + Pf6N2E2_5367 0.76 -1.1 6,167,656 + Pf6N2E2_5367 0.76 -0.5 6,167,657 - Pf6N2E2_5367 0.76 -0.3 6,167,657 - Pf6N2E2_5367 0.76 -1.0 6,167,717 - Pf6N2E2_5367 0.78 -1.9 6,167,851 + Pf6N2E2_5367 0.83 +0.2 6,167,851 + Pf6N2E2_5367 0.83 -1.7 6,167,851 + Pf6N2E2_5367 0.83 +0.8 6,167,851 + Pf6N2E2_5367 0.83 -0.2 6,167,851 + Pf6N2E2_5367 0.83 +0.3 6,167,851 + Pf6N2E2_5367 0.83 -0.4 6,167,852 - Pf6N2E2_5367 0.83 +0.8 6,167,852 - Pf6N2E2_5367 0.83 +0.1 6,167,862 + Pf6N2E2_5367 0.83 +0.5 6,167,862 + Pf6N2E2_5367 0.83 -0.7 6,167,908 + Pf6N2E2_5367 0.85 -2.1 6,167,908 + Pf6N2E2_5367 0.85 +0.2 6,168,518 + Pf6N2E2_5368 0.11 -0.3 6,168,518 + Pf6N2E2_5368 0.11 -0.3 6,168,518 + Pf6N2E2_5368 0.11 -0.3 6,168,518 + Pf6N2E2_5368 0.11 +0.5 6,168,518 + Pf6N2E2_5368 0.11 +1.8 6,168,518 + Pf6N2E2_5368 0.11 +0.8 6,168,518 + Pf6N2E2_5368 0.11 -2.2 6,168,518 + Pf6N2E2_5368 0.11 +0.2 6,168,518 + Pf6N2E2_5368 0.11 -2.5 6,168,518 + Pf6N2E2_5368 0.11 -0.3 6,168,518 + Pf6N2E2_5368 0.11 -0.3 6,168,518 + Pf6N2E2_5368 0.11 +0.7 6,168,518 + Pf6N2E2_5368 0.11 +0.1 6,168,518 + Pf6N2E2_5368 0.11 -1.6 6,168,518 + Pf6N2E2_5368 0.11 -0.3 6,168,519 - Pf6N2E2_5368 0.11 -0.2 6,168,519 - Pf6N2E2_5368 0.11 +0.5 6,168,519 - Pf6N2E2_5368 0.11 -0.9 6,168,519 - Pf6N2E2_5368 0.11 +0.5 6,168,519 - Pf6N2E2_5368 0.11 +0.4 6,168,519 - Pf6N2E2_5368 0.11 -0.9 6,168,519 - Pf6N2E2_5368 0.11 -0.7 6,168,519 - Pf6N2E2_5368 0.11 -0.7 6,168,519 - Pf6N2E2_5368 0.11 -1.6 6,168,519 - Pf6N2E2_5368 0.11 -0.4 6,168,519 - Pf6N2E2_5368 0.11 -1.0 6,168,534 + Pf6N2E2_5368 0.13 -0.3 6,168,819 + Pf6N2E2_5368 0.37 +0.2 6,168,820 - Pf6N2E2_5368 0.37 +0.6 6,168,820 - Pf6N2E2_5368 0.37 -0.5 6,169,023 + Pf6N2E2_5368 0.55 -0.5 6,169,113 + Pf6N2E2_5368 0.63 +0.7 6,169,114 - Pf6N2E2_5368 0.63 -0.0 6,169,114 - Pf6N2E2_5368 0.63 -2.1
Or see this region's nucleotide sequence