Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_473 and Pf6N2E2_474 are separated by 82 nucleotides Pf6N2E2_474 and Pf6N2E2_475 are separated by 76 nucleotides Pf6N2E2_475 and Pf6N2E2_476 are separated by 110 nucleotides
Pf6N2E2_473 - putative cytochrome p450 oxidoreductase, at 538,136 to 539,272
_473
Pf6N2E2_474 - Transcriptional regulator, ArsR family, at 539,355 to 540,110
_474
Pf6N2E2_475 - Transcriptional regulator, AraC family, at 540,187 to 540,981
_475
Pf6N2E2_476 - Permease of the drug/metabolite transporter (DMT) superfamily, at 541,092 to 542,021
_476
Position (kb) 540
541 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 539.227 kb on - strand at 539.309 kb on + strand at 539.309 kb on + strand at 539.309 kb on + strand at 539.309 kb on + strand at 539.309 kb on + strand at 539.309 kb on + strand at 539.309 kb on + strand at 539.310 kb on - strand at 539.310 kb on - strand at 539.312 kb on + strand at 539.577 kb on + strand, within Pf6N2E2_474 at 539.577 kb on + strand, within Pf6N2E2_474 at 539.578 kb on - strand, within Pf6N2E2_474 at 539.578 kb on - strand, within Pf6N2E2_474 at 539.578 kb on - strand, within Pf6N2E2_474 at 539.697 kb on + strand, within Pf6N2E2_474 at 539.705 kb on + strand, within Pf6N2E2_474 at 539.706 kb on - strand, within Pf6N2E2_474 at 540.198 kb on + strand at 540.198 kb on + strand at 540.710 kb on + strand, within Pf6N2E2_475 at 540.711 kb on - strand, within Pf6N2E2_475 at 540.711 kb on - strand, within Pf6N2E2_475 at 540.800 kb on + strand, within Pf6N2E2_475 at 540.801 kb on - strand, within Pf6N2E2_475 at 540.801 kb on - strand, within Pf6N2E2_475 at 540.839 kb on + strand, within Pf6N2E2_475 at 540.839 kb on + strand, within Pf6N2E2_475 at 540.839 kb on + strand, within Pf6N2E2_475 at 540.840 kb on - strand, within Pf6N2E2_475 at 540.840 kb on - strand, within Pf6N2E2_475 at 540.840 kb on - strand, within Pf6N2E2_475 at 540.840 kb on - strand, within Pf6N2E2_475 at 540.840 kb on - strand, within Pf6N2E2_475 at 540.845 kb on + strand, within Pf6N2E2_475 at 540.882 kb on - strand, within Pf6N2E2_475 at 540.981 kb on + strand at 541.028 kb on + strand at 541.028 kb on + strand at 541.029 kb on - strand at 541.029 kb on - strand at 541.029 kb on - strand at 541.029 kb on - strand at 541.051 kb on - strand at 541.373 kb on + strand, within Pf6N2E2_476 at 541.373 kb on + strand, within Pf6N2E2_476 at 541.405 kb on - strand, within Pf6N2E2_476 at 541.615 kb on + strand, within Pf6N2E2_476 at 541.616 kb on - strand, within Pf6N2E2_476 at 541.919 kb on + strand, within Pf6N2E2_476
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 539,227 - +1.0 539,309 + -0.2 539,309 + +1.9 539,309 + -0.4 539,309 + -1.1 539,309 + -0.2 539,309 + +0.2 539,309 + -0.6 539,310 - -1.0 539,310 - -0.2 539,312 + -2.0 539,577 + Pf6N2E2_474 0.29 +0.1 539,577 + Pf6N2E2_474 0.29 -0.1 539,578 - Pf6N2E2_474 0.29 -1.4 539,578 - Pf6N2E2_474 0.29 -1.1 539,578 - Pf6N2E2_474 0.29 +0.1 539,697 + Pf6N2E2_474 0.45 +0.2 539,705 + Pf6N2E2_474 0.46 +0.4 539,706 - Pf6N2E2_474 0.46 +0.2 540,198 + +0.5 540,198 + -2.3 540,710 + Pf6N2E2_475 0.66 -0.4 540,711 - Pf6N2E2_475 0.66 +0.2 540,711 - Pf6N2E2_475 0.66 +0.6 540,800 + Pf6N2E2_475 0.77 -1.1 540,801 - Pf6N2E2_475 0.77 -0.9 540,801 - Pf6N2E2_475 0.77 -0.4 540,839 + Pf6N2E2_475 0.82 -1.4 540,839 + Pf6N2E2_475 0.82 -0.5 540,839 + Pf6N2E2_475 0.82 -0.1 540,840 - Pf6N2E2_475 0.82 -1.0 540,840 - Pf6N2E2_475 0.82 -0.7 540,840 - Pf6N2E2_475 0.82 -0.2 540,840 - Pf6N2E2_475 0.82 +0.0 540,840 - Pf6N2E2_475 0.82 +0.3 540,845 + Pf6N2E2_475 0.83 -0.6 540,882 - Pf6N2E2_475 0.87 -0.4 540,981 + -0.3 541,028 + -0.1 541,028 + +0.7 541,029 - +0.4 541,029 - +0.2 541,029 - -0.3 541,029 - -0.9 541,051 - -2.8 541,373 + Pf6N2E2_476 0.30 -0.4 541,373 + Pf6N2E2_476 0.30 -0.1 541,405 - Pf6N2E2_476 0.34 +0.6 541,615 + Pf6N2E2_476 0.56 -1.2 541,616 - Pf6N2E2_476 0.56 +0.6 541,919 + Pf6N2E2_476 0.89 -0.5
Or see this region's nucleotide sequence