Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4544 and Pf6N2E2_4545 are separated by 25 nucleotides Pf6N2E2_4545 and Pf6N2E2_4546 overlap by 14 nucleotides Pf6N2E2_4546 and Pf6N2E2_4547 are separated by 542 nucleotides
Pf6N2E2_4544 - Type III secretion outermembrane pore forming protein (YscC,MxiD,HrcC, InvG), at 5,275,252 to 5,277,369
_4544
Pf6N2E2_4545 - hypothetical protein, at 5,277,395 to 5,277,832
_4545
Pf6N2E2_4546 - hypothetical protein, at 5,277,819 to 5,278,043
_4546
Pf6N2E2_4547 - Type III secretion cytoplasmic protein (YscL), at 5,278,586 to 5,279,179
_4547
Position (kb)
5277
5278 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5276.465 kb on + strand, within Pf6N2E2_4544 at 5276.465 kb on + strand, within Pf6N2E2_4544 at 5276.466 kb on - strand, within Pf6N2E2_4544 at 5276.466 kb on - strand, within Pf6N2E2_4544 at 5276.750 kb on + strand, within Pf6N2E2_4544 at 5276.751 kb on - strand, within Pf6N2E2_4544 at 5276.861 kb on + strand, within Pf6N2E2_4544 at 5276.862 kb on - strand, within Pf6N2E2_4544 at 5276.862 kb on - strand, within Pf6N2E2_4544 at 5277.026 kb on + strand, within Pf6N2E2_4544 at 5277.027 kb on - strand, within Pf6N2E2_4544 at 5277.027 kb on - strand, within Pf6N2E2_4544 at 5277.027 kb on - strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.042 kb on - strand, within Pf6N2E2_4544 at 5277.042 kb on - strand, within Pf6N2E2_4544 at 5277.042 kb on - strand, within Pf6N2E2_4544 at 5277.042 kb on - strand, within Pf6N2E2_4544 at 5277.042 kb on - strand, within Pf6N2E2_4544 at 5277.042 kb on - strand, within Pf6N2E2_4544 at 5277.148 kb on + strand, within Pf6N2E2_4544 at 5277.168 kb on + strand at 5277.169 kb on - strand at 5277.187 kb on + strand at 5277.219 kb on - strand at 5277.224 kb on + strand at 5277.224 kb on + strand at 5277.224 kb on + strand at 5277.352 kb on + strand at 5277.352 kb on + strand at 5277.352 kb on + strand at 5277.352 kb on + strand at 5277.352 kb on + strand at 5277.353 kb on - strand at 5277.377 kb on + strand at 5277.478 kb on + strand, within Pf6N2E2_4545 at 5278.003 kb on + strand, within Pf6N2E2_4546 at 5278.003 kb on + strand, within Pf6N2E2_4546 at 5278.003 kb on + strand, within Pf6N2E2_4546 at 5278.003 kb on + strand, within Pf6N2E2_4546 at 5278.004 kb on - strand, within Pf6N2E2_4546 at 5278.004 kb on - strand, within Pf6N2E2_4546 at 5278.004 kb on - strand, within Pf6N2E2_4546 at 5278.004 kb on - strand, within Pf6N2E2_4546 at 5278.004 kb on - strand, within Pf6N2E2_4546 at 5278.068 kb on + strand at 5278.068 kb on + strand at 5278.068 kb on + strand at 5278.090 kb on + strand at 5278.091 kb on - strand at 5278.215 kb on - strand at 5278.215 kb on - strand at 5278.215 kb on - strand at 5278.215 kb on - strand at 5278.215 kb on - strand at 5278.215 kb on - strand at 5278.219 kb on - strand at 5278.219 kb on - strand at 5278.244 kb on + strand at 5278.244 kb on + strand at 5278.381 kb on - strand at 5278.381 kb on - strand at 5278.443 kb on + strand at 5278.445 kb on + strand at 5278.445 kb on + strand at 5278.493 kb on + strand at 5278.493 kb on + strand at 5278.493 kb on + strand at 5278.493 kb on + strand at 5278.493 kb on + strand at 5278.493 kb on + strand at 5278.494 kb on - strand at 5278.530 kb on + strand at 5278.531 kb on - strand at 5278.531 kb on - strand at 5278.531 kb on - strand at 5278.622 kb on + strand at 5278.656 kb on + strand, within Pf6N2E2_4547 at 5278.656 kb on + strand, within Pf6N2E2_4547 at 5278.656 kb on + strand, within Pf6N2E2_4547 at 5278.656 kb on + strand, within Pf6N2E2_4547 at 5278.656 kb on + strand, within Pf6N2E2_4547 at 5278.656 kb on + strand, within Pf6N2E2_4547 at 5278.656 kb on + strand, within Pf6N2E2_4547 at 5278.657 kb on - strand, within Pf6N2E2_4547 at 5278.657 kb on - strand, within Pf6N2E2_4547 at 5278.657 kb on - strand, within Pf6N2E2_4547 at 5278.657 kb on - strand, within Pf6N2E2_4547 at 5278.660 kb on + strand, within Pf6N2E2_4547 at 5278.660 kb on + strand, within Pf6N2E2_4547 at 5278.660 kb on + strand, within Pf6N2E2_4547 at 5278.660 kb on + strand, within Pf6N2E2_4547 at 5278.660 kb on + strand, within Pf6N2E2_4547 at 5278.661 kb on - strand, within Pf6N2E2_4547 at 5278.661 kb on - strand, within Pf6N2E2_4547
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 5,276,465 + Pf6N2E2_4544 0.57 -0.2 5,276,465 + Pf6N2E2_4544 0.57 -0.6 5,276,466 - Pf6N2E2_4544 0.57 -0.3 5,276,466 - Pf6N2E2_4544 0.57 +0.7 5,276,750 + Pf6N2E2_4544 0.71 -0.4 5,276,751 - Pf6N2E2_4544 0.71 -0.4 5,276,861 + Pf6N2E2_4544 0.76 -0.1 5,276,862 - Pf6N2E2_4544 0.76 -0.5 5,276,862 - Pf6N2E2_4544 0.76 +0.8 5,277,026 + Pf6N2E2_4544 0.84 -1.5 5,277,027 - Pf6N2E2_4544 0.84 +0.2 5,277,027 - Pf6N2E2_4544 0.84 -0.5 5,277,027 - Pf6N2E2_4544 0.84 +0.4 5,277,041 + Pf6N2E2_4544 0.84 -0.3 5,277,041 + Pf6N2E2_4544 0.84 +0.4 5,277,041 + Pf6N2E2_4544 0.84 +0.2 5,277,041 + Pf6N2E2_4544 0.84 +0.5 5,277,041 + Pf6N2E2_4544 0.84 -0.1 5,277,041 + Pf6N2E2_4544 0.84 +0.2 5,277,041 + Pf6N2E2_4544 0.84 -0.1 5,277,042 - Pf6N2E2_4544 0.85 -0.1 5,277,042 - Pf6N2E2_4544 0.85 -0.6 5,277,042 - Pf6N2E2_4544 0.85 +0.2 5,277,042 - Pf6N2E2_4544 0.85 +0.5 5,277,042 - Pf6N2E2_4544 0.85 -0.0 5,277,042 - Pf6N2E2_4544 0.85 +0.0 5,277,148 + Pf6N2E2_4544 0.90 +0.2 5,277,168 + +0.5 5,277,169 - -1.5 5,277,187 + -1.2 5,277,219 - -0.4 5,277,224 + -1.1 5,277,224 + +0.1 5,277,224 + -0.3 5,277,352 + -0.4 5,277,352 + +0.1 5,277,352 + -0.3 5,277,352 + +0.3 5,277,352 + -0.1 5,277,353 - -0.6 5,277,377 + +0.1 5,277,478 + Pf6N2E2_4545 0.19 -0.9 5,278,003 + Pf6N2E2_4546 0.82 -0.6 5,278,003 + Pf6N2E2_4546 0.82 +3.0 5,278,003 + Pf6N2E2_4546 0.82 -0.8 5,278,003 + Pf6N2E2_4546 0.82 +0.4 5,278,004 - Pf6N2E2_4546 0.82 -0.2 5,278,004 - Pf6N2E2_4546 0.82 +0.4 5,278,004 - Pf6N2E2_4546 0.82 -2.9 5,278,004 - Pf6N2E2_4546 0.82 -0.8 5,278,004 - Pf6N2E2_4546 0.82 -1.5 5,278,068 + +1.3 5,278,068 + -0.8 5,278,068 + -0.4 5,278,090 + +0.2 5,278,091 - -0.6 5,278,215 - +0.2 5,278,215 - -0.7 5,278,215 - +1.0 5,278,215 - +0.5 5,278,215 - +0.7 5,278,215 - +0.5 5,278,219 - +1.3 5,278,219 - -0.2 5,278,244 + -0.9 5,278,244 + -0.6 5,278,381 - -1.7 5,278,381 - +0.1 5,278,443 + -1.4 5,278,445 + -0.3 5,278,445 + -0.8 5,278,493 + +0.5 5,278,493 + -0.1 5,278,493 + +1.4 5,278,493 + -0.0 5,278,493 + +0.3 5,278,493 + +0.2 5,278,494 - -0.5 5,278,530 + -0.0 5,278,531 - +0.9 5,278,531 - -0.2 5,278,531 - -1.8 5,278,622 + -0.1 5,278,656 + Pf6N2E2_4547 0.12 -0.2 5,278,656 + Pf6N2E2_4547 0.12 +0.5 5,278,656 + Pf6N2E2_4547 0.12 +0.1 5,278,656 + Pf6N2E2_4547 0.12 +1.2 5,278,656 + Pf6N2E2_4547 0.12 -1.1 5,278,656 + Pf6N2E2_4547 0.12 -0.3 5,278,656 + Pf6N2E2_4547 0.12 +0.1 5,278,657 - Pf6N2E2_4547 0.12 +0.4 5,278,657 - Pf6N2E2_4547 0.12 -0.1 5,278,657 - Pf6N2E2_4547 0.12 -0.0 5,278,657 - Pf6N2E2_4547 0.12 -0.3 5,278,660 + Pf6N2E2_4547 0.12 -0.1 5,278,660 + Pf6N2E2_4547 0.12 -2.5 5,278,660 + Pf6N2E2_4547 0.12 +0.9 5,278,660 + Pf6N2E2_4547 0.12 -0.1 5,278,660 + Pf6N2E2_4547 0.12 -0.1 5,278,661 - Pf6N2E2_4547 0.13 -0.3 5,278,661 - Pf6N2E2_4547 0.13 +0.1
Or see this region's nucleotide sequence