Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4190 and Pf6N2E2_4191 are separated by 10 nucleotides Pf6N2E2_4191 and Pf6N2E2_4192 are separated by 80 nucleotides Pf6N2E2_4192 and Pf6N2E2_4193 are separated by 177 nucleotides
Pf6N2E2_4190 - Glycyl-tRNA synthetase beta chain (EC 6.1.1.14), at 4,874,822 to 4,876,873
_4190
Pf6N2E2_4191 - D-glycero-D-manno-heptose 1,7-bisphosphate phosphatase (EC 3.1.1.-); Histidinol-phosphatase (EC 3.1.3.15), at 4,876,884 to 4,877,417
_4191
Pf6N2E2_4192 - 1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 4,877,498 to 4,878,277
_4192
Pf6N2E2_4193 - Signal transduction histidine kinase, at 4,878,455 to 4,879,972
_4193
Position (kb)
4877
4878
4879 Strain fitness (log2 ratio)
-2
-1
0
1 at 4877.415 kb on + strand at 4877.415 kb on + strand at 4877.497 kb on + strand at 4877.497 kb on + strand at 4877.633 kb on + strand at 4878.138 kb on - strand at 4878.389 kb on - strand at 4878.389 kb on - strand at 4878.389 kb on - strand at 4878.484 kb on + strand at 4878.511 kb on + strand at 4878.512 kb on - strand at 4878.620 kb on - strand, within Pf6N2E2_4193 at 4878.620 kb on - strand, within Pf6N2E2_4193 at 4878.632 kb on + strand, within Pf6N2E2_4193 at 4878.632 kb on + strand, within Pf6N2E2_4193 at 4878.632 kb on + strand, within Pf6N2E2_4193 at 4878.632 kb on + strand, within Pf6N2E2_4193 at 4878.633 kb on - strand, within Pf6N2E2_4193 at 4878.633 kb on - strand, within Pf6N2E2_4193 at 4878.633 kb on - strand, within Pf6N2E2_4193 at 4878.633 kb on - strand, within Pf6N2E2_4193 at 4878.633 kb on - strand, within Pf6N2E2_4193 at 4878.735 kb on + strand, within Pf6N2E2_4193 at 4878.735 kb on + strand, within Pf6N2E2_4193 at 4878.735 kb on + strand, within Pf6N2E2_4193 at 4878.735 kb on + strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.818 kb on + strand, within Pf6N2E2_4193 at 4878.818 kb on + strand, within Pf6N2E2_4193 at 4878.818 kb on + strand, within Pf6N2E2_4193 at 4878.818 kb on + strand, within Pf6N2E2_4193 at 4878.818 kb on + strand, within Pf6N2E2_4193 at 4878.819 kb on - strand, within Pf6N2E2_4193 at 4878.819 kb on - strand, within Pf6N2E2_4193 at 4878.819 kb on - strand, within Pf6N2E2_4193 at 4878.918 kb on + strand, within Pf6N2E2_4193 at 4878.918 kb on + strand, within Pf6N2E2_4193 at 4878.980 kb on + strand, within Pf6N2E2_4193 at 4878.980 kb on + strand, within Pf6N2E2_4193 at 4878.981 kb on - strand, within Pf6N2E2_4193 at 4878.981 kb on - strand, within Pf6N2E2_4193 at 4878.981 kb on - strand, within Pf6N2E2_4193 at 4879.013 kb on - strand, within Pf6N2E2_4193
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,877,415 + +0.2 4,877,415 + -0.7 4,877,497 + -0.5 4,877,497 + -2.4 4,877,633 + +1.5 4,878,138 - +0.1 4,878,389 - -1.1 4,878,389 - -0.9 4,878,389 - +0.2 4,878,484 + -0.5 4,878,511 + +1.0 4,878,512 - -0.4 4,878,620 - Pf6N2E2_4193 0.11 -0.6 4,878,620 - Pf6N2E2_4193 0.11 -0.3 4,878,632 + Pf6N2E2_4193 0.12 -0.6 4,878,632 + Pf6N2E2_4193 0.12 +0.5 4,878,632 + Pf6N2E2_4193 0.12 +1.2 4,878,632 + Pf6N2E2_4193 0.12 +0.7 4,878,633 - Pf6N2E2_4193 0.12 -0.7 4,878,633 - Pf6N2E2_4193 0.12 +0.8 4,878,633 - Pf6N2E2_4193 0.12 -0.2 4,878,633 - Pf6N2E2_4193 0.12 -2.5 4,878,633 - Pf6N2E2_4193 0.12 -1.3 4,878,735 + Pf6N2E2_4193 0.18 +0.5 4,878,735 + Pf6N2E2_4193 0.18 -0.3 4,878,735 + Pf6N2E2_4193 0.18 +0.6 4,878,735 + Pf6N2E2_4193 0.18 +0.7 4,878,736 - Pf6N2E2_4193 0.19 -0.4 4,878,736 - Pf6N2E2_4193 0.19 -0.9 4,878,736 - Pf6N2E2_4193 0.19 -0.2 4,878,736 - Pf6N2E2_4193 0.19 -1.0 4,878,736 - Pf6N2E2_4193 0.19 -0.7 4,878,736 - Pf6N2E2_4193 0.19 +0.3 4,878,736 - Pf6N2E2_4193 0.19 -0.3 4,878,736 - Pf6N2E2_4193 0.19 -0.3 4,878,736 - Pf6N2E2_4193 0.19 +0.2 4,878,736 - Pf6N2E2_4193 0.19 -1.4 4,878,736 - Pf6N2E2_4193 0.19 +0.7 4,878,736 - Pf6N2E2_4193 0.19 +0.2 4,878,818 + Pf6N2E2_4193 0.24 +0.1 4,878,818 + Pf6N2E2_4193 0.24 +0.7 4,878,818 + Pf6N2E2_4193 0.24 -0.2 4,878,818 + Pf6N2E2_4193 0.24 +1.3 4,878,818 + Pf6N2E2_4193 0.24 +0.2 4,878,819 - Pf6N2E2_4193 0.24 -0.7 4,878,819 - Pf6N2E2_4193 0.24 -0.3 4,878,819 - Pf6N2E2_4193 0.24 +0.6 4,878,918 + Pf6N2E2_4193 0.31 -1.1 4,878,918 + Pf6N2E2_4193 0.31 -2.6 4,878,980 + Pf6N2E2_4193 0.35 +0.0 4,878,980 + Pf6N2E2_4193 0.35 +0.1 4,878,981 - Pf6N2E2_4193 0.35 -0.6 4,878,981 - Pf6N2E2_4193 0.35 -1.4 4,878,981 - Pf6N2E2_4193 0.35 -1.7 4,879,013 - Pf6N2E2_4193 0.37 -0.6
Or see this region's nucleotide sequence