Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_383 and Pf6N2E2_384 are separated by 58 nucleotides Pf6N2E2_384 and Pf6N2E2_385 are separated by 97 nucleotides Pf6N2E2_385 and Pf6N2E2_386 are separated by 460 nucleotides
Pf6N2E2_383 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 450,812 to 451,579
_383
Pf6N2E2_384 - Glycine cleavage system transcriptional activator, at 451,638 to 451,979
_384
Pf6N2E2_385 - hypothetical protein, at 452,077 to 452,247
_385
Pf6N2E2_386 - similar to ribulose-1,5-bisphosphate carboxylase, Type III, at 452,708 to 453,985
_386
Position (kb) 451
452 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 450.757 kb on + strand at 450.792 kb on + strand at 450.795 kb on + strand at 450.795 kb on + strand at 450.795 kb on + strand at 450.795 kb on + strand at 450.796 kb on - strand at 450.840 kb on + strand at 450.857 kb on - strand at 450.909 kb on + strand, within Pf6N2E2_383 at 450.909 kb on + strand, within Pf6N2E2_383 at 450.910 kb on - strand, within Pf6N2E2_383 at 450.929 kb on - strand, within Pf6N2E2_383 at 451.201 kb on + strand, within Pf6N2E2_383 at 451.201 kb on + strand, within Pf6N2E2_383 at 451.201 kb on + strand, within Pf6N2E2_383 at 451.202 kb on - strand, within Pf6N2E2_383 at 451.427 kb on + strand, within Pf6N2E2_383 at 451.428 kb on - strand, within Pf6N2E2_383 at 451.490 kb on + strand, within Pf6N2E2_383 at 451.490 kb on + strand, within Pf6N2E2_383 at 451.491 kb on - strand, within Pf6N2E2_383 at 451.515 kb on - strand at 451.515 kb on - strand at 451.590 kb on + strand at 451.591 kb on - strand at 451.597 kb on - strand at 451.616 kb on + strand at 451.616 kb on + strand at 451.660 kb on - strand at 451.852 kb on + strand at 451.853 kb on - strand, within Pf6N2E2_384 at 451.853 kb on - strand, within Pf6N2E2_384 at 451.913 kb on - strand, within Pf6N2E2_384 at 452.005 kb on + strand at 452.005 kb on + strand at 452.006 kb on - strand at 452.019 kb on + strand at 452.019 kb on + strand at 452.020 kb on - strand at 452.020 kb on - strand at 452.021 kb on + strand at 452.021 kb on + strand at 452.022 kb on - strand at 452.022 kb on - strand at 452.264 kb on + strand at 452.405 kb on + strand at 452.635 kb on - strand at 452.635 kb on - strand at 452.637 kb on - strand at 452.678 kb on - strand at 452.678 kb on - strand at 452.679 kb on + strand at 452.758 kb on - strand at 452.819 kb on - strand at 452.819 kb on - strand at 452.919 kb on + strand, within Pf6N2E2_386 at 452.924 kb on - strand, within Pf6N2E2_386 at 452.931 kb on - strand, within Pf6N2E2_386 at 452.931 kb on - strand, within Pf6N2E2_386 at 452.931 kb on - strand, within Pf6N2E2_386 at 452.931 kb on - strand, within Pf6N2E2_386 at 452.960 kb on + strand, within Pf6N2E2_386 at 452.960 kb on + strand, within Pf6N2E2_386 at 452.960 kb on + strand, within Pf6N2E2_386 at 452.960 kb on + strand, within Pf6N2E2_386 at 452.961 kb on - strand, within Pf6N2E2_386 at 452.961 kb on - strand, within Pf6N2E2_386 at 452.964 kb on - strand, within Pf6N2E2_386
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 450,757 + +0.7 450,792 + +1.2 450,795 + +0.2 450,795 + -0.2 450,795 + -1.9 450,795 + -0.7 450,796 - +0.1 450,840 + -0.6 450,857 - -0.2 450,909 + Pf6N2E2_383 0.13 +0.1 450,909 + Pf6N2E2_383 0.13 -0.9 450,910 - Pf6N2E2_383 0.13 -1.8 450,929 - Pf6N2E2_383 0.15 -2.0 451,201 + Pf6N2E2_383 0.51 +0.1 451,201 + Pf6N2E2_383 0.51 +0.0 451,201 + Pf6N2E2_383 0.51 +0.4 451,202 - Pf6N2E2_383 0.51 +0.3 451,427 + Pf6N2E2_383 0.80 -0.6 451,428 - Pf6N2E2_383 0.80 +0.3 451,490 + Pf6N2E2_383 0.88 +0.3 451,490 + Pf6N2E2_383 0.88 -0.1 451,491 - Pf6N2E2_383 0.88 -1.2 451,515 - +0.6 451,515 - -1.1 451,590 + -0.5 451,591 - -0.2 451,597 - +0.2 451,616 + -0.6 451,616 + -1.1 451,660 - +0.7 451,852 + +0.6 451,853 - Pf6N2E2_384 0.63 -0.3 451,853 - Pf6N2E2_384 0.63 -0.6 451,913 - Pf6N2E2_384 0.80 -1.7 452,005 + +0.6 452,005 + +1.8 452,006 - -0.1 452,019 + +1.0 452,019 + -0.7 452,020 - +1.3 452,020 - -0.2 452,021 + +0.9 452,021 + +0.7 452,022 - +0.0 452,022 - -0.9 452,264 + -1.2 452,405 + -1.0 452,635 - -0.4 452,635 - -1.0 452,637 - +1.1 452,678 - +1.6 452,678 - -0.7 452,679 + +0.1 452,758 - -0.6 452,819 - -1.8 452,819 - +1.1 452,919 + Pf6N2E2_386 0.17 -0.2 452,924 - Pf6N2E2_386 0.17 -0.3 452,931 - Pf6N2E2_386 0.17 -0.0 452,931 - Pf6N2E2_386 0.17 -0.1 452,931 - Pf6N2E2_386 0.17 -0.7 452,931 - Pf6N2E2_386 0.17 -0.5 452,960 + Pf6N2E2_386 0.20 -0.8 452,960 + Pf6N2E2_386 0.20 -1.9 452,960 + Pf6N2E2_386 0.20 +2.7 452,960 + Pf6N2E2_386 0.20 -0.5 452,961 - Pf6N2E2_386 0.20 -0.6 452,961 - Pf6N2E2_386 0.20 -1.2 452,964 - Pf6N2E2_386 0.20 -2.9
Or see this region's nucleotide sequence