Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3832 and Pf6N2E2_3833 are separated by 177 nucleotides Pf6N2E2_3833 and Pf6N2E2_3834 overlap by 45 nucleotides
Pf6N2E2_3832 - 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC 5.4.2.1), at 4,465,261 to 4,466,760
_3832
Pf6N2E2_3833 - hypothetical protein, at 4,466,938 to 4,467,123
_3833
Pf6N2E2_3834 - Lipoprotein NlpD, at 4,467,079 to 4,468,371
_3834
Position (kb)
4466
4467
4468 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4466.769 kb on + strand at 4466.770 kb on - strand at 4466.770 kb on - strand at 4466.770 kb on - strand at 4466.770 kb on - strand at 4466.880 kb on + strand at 4467.042 kb on + strand, within Pf6N2E2_3833 at 4467.042 kb on + strand, within Pf6N2E2_3833 at 4467.043 kb on - strand, within Pf6N2E2_3833 at 4467.043 kb on - strand, within Pf6N2E2_3833 at 4467.045 kb on + strand, within Pf6N2E2_3833 at 4467.045 kb on + strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.078 kb on + strand, within Pf6N2E2_3833 at 4467.079 kb on - strand at 4467.079 kb on - strand at 4467.440 kb on - strand, within Pf6N2E2_3834 at 4467.440 kb on - strand, within Pf6N2E2_3834 at 4467.496 kb on + strand, within Pf6N2E2_3834 at 4467.497 kb on - strand, within Pf6N2E2_3834 at 4467.497 kb on - strand, within Pf6N2E2_3834 at 4467.499 kb on + strand, within Pf6N2E2_3834 at 4467.499 kb on + strand, within Pf6N2E2_3834 at 4467.500 kb on - strand, within Pf6N2E2_3834 at 4467.500 kb on - strand, within Pf6N2E2_3834 at 4468.016 kb on + strand, within Pf6N2E2_3834 at 4468.016 kb on + strand, within Pf6N2E2_3834 at 4468.016 kb on + strand, within Pf6N2E2_3834 at 4468.016 kb on + strand, within Pf6N2E2_3834 at 4468.019 kb on + strand, within Pf6N2E2_3834 at 4468.020 kb on - strand, within Pf6N2E2_3834
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,466,769 + +1.2 4,466,770 - -1.9 4,466,770 - -0.5 4,466,770 - -0.1 4,466,770 - -0.8 4,466,880 + +0.2 4,467,042 + Pf6N2E2_3833 0.56 -0.5 4,467,042 + Pf6N2E2_3833 0.56 -0.5 4,467,043 - Pf6N2E2_3833 0.56 -3.8 4,467,043 - Pf6N2E2_3833 0.56 -1.7 4,467,045 + Pf6N2E2_3833 0.58 -1.1 4,467,045 + Pf6N2E2_3833 0.58 -1.0 4,467,046 - Pf6N2E2_3833 0.58 -2.8 4,467,046 - Pf6N2E2_3833 0.58 -3.9 4,467,046 - Pf6N2E2_3833 0.58 -4.0 4,467,046 - Pf6N2E2_3833 0.58 -3.0 4,467,046 - Pf6N2E2_3833 0.58 -2.9 4,467,078 + Pf6N2E2_3833 0.75 -0.9 4,467,079 - -3.3 4,467,079 - -3.6 4,467,440 - Pf6N2E2_3834 0.28 -2.1 4,467,440 - Pf6N2E2_3834 0.28 -4.1 4,467,496 + Pf6N2E2_3834 0.32 -2.6 4,467,497 - Pf6N2E2_3834 0.32 -0.7 4,467,497 - Pf6N2E2_3834 0.32 -1.9 4,467,499 + Pf6N2E2_3834 0.32 -0.7 4,467,499 + Pf6N2E2_3834 0.32 -0.8 4,467,500 - Pf6N2E2_3834 0.33 -3.0 4,467,500 - Pf6N2E2_3834 0.33 -1.8 4,468,016 + Pf6N2E2_3834 0.72 -0.2 4,468,016 + Pf6N2E2_3834 0.72 +0.1 4,468,016 + Pf6N2E2_3834 0.72 -0.4 4,468,016 + Pf6N2E2_3834 0.72 -0.8 4,468,019 + Pf6N2E2_3834 0.73 +0.1 4,468,020 - Pf6N2E2_3834 0.73 -2.7
Or see this region's nucleotide sequence