Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3694 and Pf6N2E2_3695 are separated by 286 nucleotides Pf6N2E2_3695 and Pf6N2E2_3696 are separated by 159 nucleotides Pf6N2E2_3696 and Pf6N2E2_3697 are separated by 133 nucleotides
Pf6N2E2_3694 - Helix-turn-helix motif, at 4,311,313 to 4,311,450
_3694
Pf6N2E2_3695 - Helix-turn-helix motif, at 4,311,737 to 4,312,138
_3695
Pf6N2E2_3696 - hypothetical protein, at 4,312,298 to 4,312,576
_3696
Pf6N2E2_3697 - Transcriptional regulator, LysR family, at 4,312,710 to 4,313,576
_3697
Position (kb) 4312
4313 Strain fitness (log2 ratio) -2
-1
0
1 at 4311.726 kb on - strand at 4311.821 kb on + strand, within Pf6N2E2_3695 at 4312.056 kb on + strand, within Pf6N2E2_3695 at 4312.056 kb on + strand, within Pf6N2E2_3695 at 4312.056 kb on + strand, within Pf6N2E2_3695 at 4312.056 kb on + strand, within Pf6N2E2_3695 at 4312.057 kb on - strand, within Pf6N2E2_3695 at 4312.169 kb on + strand at 4312.170 kb on - strand at 4312.175 kb on + strand at 4312.175 kb on + strand at 4312.175 kb on + strand at 4312.175 kb on + strand at 4312.175 kb on + strand at 4312.175 kb on + strand at 4312.175 kb on + strand at 4312.175 kb on + strand at 4312.175 kb on + strand at 4312.176 kb on - strand at 4312.176 kb on - strand at 4312.176 kb on - strand at 4312.204 kb on + strand at 4312.238 kb on + strand at 4312.239 kb on - strand at 4312.259 kb on - strand at 4312.309 kb on + strand at 4312.310 kb on - strand at 4312.464 kb on + strand, within Pf6N2E2_3696 at 4312.600 kb on + strand at 4312.600 kb on + strand at 4312.600 kb on + strand at 4312.600 kb on + strand at 4312.600 kb on + strand at 4312.601 kb on - strand at 4312.601 kb on - strand at 4312.601 kb on - strand at 4312.601 kb on - strand at 4312.761 kb on - strand at 4313.015 kb on - strand, within Pf6N2E2_3697 at 4313.015 kb on - strand, within Pf6N2E2_3697 at 4313.085 kb on + strand, within Pf6N2E2_3697 at 4313.099 kb on - strand, within Pf6N2E2_3697 at 4313.129 kb on + strand, within Pf6N2E2_3697 at 4313.129 kb on + strand, within Pf6N2E2_3697 at 4313.129 kb on + strand, within Pf6N2E2_3697 at 4313.130 kb on - strand, within Pf6N2E2_3697 at 4313.130 kb on - strand, within Pf6N2E2_3697 at 4313.303 kb on + strand, within Pf6N2E2_3697 at 4313.303 kb on + strand, within Pf6N2E2_3697 at 4313.303 kb on + strand, within Pf6N2E2_3697 at 4313.304 kb on - strand, within Pf6N2E2_3697 at 4313.304 kb on - strand, within Pf6N2E2_3697 at 4313.419 kb on + strand, within Pf6N2E2_3697 at 4313.422 kb on + strand, within Pf6N2E2_3697 at 4313.423 kb on - strand, within Pf6N2E2_3697 at 4313.423 kb on - strand, within Pf6N2E2_3697
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,311,726 - -0.8 4,311,821 + Pf6N2E2_3695 0.21 -0.8 4,312,056 + Pf6N2E2_3695 0.79 +0.8 4,312,056 + Pf6N2E2_3695 0.79 +0.6 4,312,056 + Pf6N2E2_3695 0.79 +0.3 4,312,056 + Pf6N2E2_3695 0.79 -0.8 4,312,057 - Pf6N2E2_3695 0.80 -1.0 4,312,169 + -0.3 4,312,170 - +0.5 4,312,175 + +0.9 4,312,175 + -0.9 4,312,175 + +0.6 4,312,175 + -0.1 4,312,175 + +0.7 4,312,175 + +0.0 4,312,175 + +0.6 4,312,175 + -0.0 4,312,175 + +1.0 4,312,176 - +0.5 4,312,176 - +0.2 4,312,176 - -1.0 4,312,204 + +0.6 4,312,238 + +0.4 4,312,239 - -1.6 4,312,259 - -0.3 4,312,309 + +0.4 4,312,310 - +0.5 4,312,464 + Pf6N2E2_3696 0.59 -0.7 4,312,600 + -0.5 4,312,600 + +0.0 4,312,600 + -0.0 4,312,600 + +0.4 4,312,600 + -0.0 4,312,601 - +0.0 4,312,601 - +0.5 4,312,601 - -0.0 4,312,601 - +1.0 4,312,761 - +0.2 4,313,015 - Pf6N2E2_3697 0.35 -0.0 4,313,015 - Pf6N2E2_3697 0.35 -0.2 4,313,085 + Pf6N2E2_3697 0.43 +0.8 4,313,099 - Pf6N2E2_3697 0.45 +0.4 4,313,129 + Pf6N2E2_3697 0.48 +0.7 4,313,129 + Pf6N2E2_3697 0.48 -0.7 4,313,129 + Pf6N2E2_3697 0.48 -0.2 4,313,130 - Pf6N2E2_3697 0.48 +0.4 4,313,130 - Pf6N2E2_3697 0.48 +1.3 4,313,303 + Pf6N2E2_3697 0.68 +0.7 4,313,303 + Pf6N2E2_3697 0.68 -1.0 4,313,303 + Pf6N2E2_3697 0.68 +0.8 4,313,304 - Pf6N2E2_3697 0.69 +0.4 4,313,304 - Pf6N2E2_3697 0.69 +0.9 4,313,419 + Pf6N2E2_3697 0.82 -0.5 4,313,422 + Pf6N2E2_3697 0.82 -0.5 4,313,423 - Pf6N2E2_3697 0.82 -2.0 4,313,423 - Pf6N2E2_3697 0.82 -1.1
Or see this region's nucleotide sequence