Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3659 and Pf6N2E2_3660 are separated by 221 nucleotides Pf6N2E2_3660 and Pf6N2E2_3661 are separated by 18 nucleotides Pf6N2E2_3661 and Pf6N2E2_3662 are separated by 192 nucleotides Pf6N2E2_3662 and Pf6N2E2_3663 are separated by 89 nucleotides
Pf6N2E2_3659 - Protein fixF, at 4,261,130 to 4,262,236
_3659
Pf6N2E2_3660 - Oligosaccharide repeat unit polymerase Wzy; O-antigen ligase, at 4,262,458 to 4,263,090
_3660
Pf6N2E2_3661 - hypothetical protein, at 4,263,109 to 4,263,969
_3661
Pf6N2E2_3662 - hypothetical protein, at 4,264,162 to 4,264,302
_3662
Pf6N2E2_3663 - Glycosyl transferase, at 4,264,392 to 4,265,294
_3663
Position (kb)
4263
4264 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7
8 at 4262.144 kb on - strand at 4262.144 kb on - strand at 4262.144 kb on - strand at 4262.147 kb on + strand at 4262.147 kb on + strand at 4262.147 kb on + strand at 4262.148 kb on - strand at 4262.148 kb on - strand at 4262.148 kb on - strand at 4262.148 kb on - strand at 4262.148 kb on - strand at 4262.148 kb on - strand at 4262.190 kb on + strand at 4262.190 kb on + strand at 4262.191 kb on - strand at 4262.191 kb on - strand at 4262.252 kb on + strand at 4262.264 kb on - strand at 4262.279 kb on + strand at 4262.279 kb on + strand at 4262.295 kb on - strand at 4262.376 kb on - strand at 4262.377 kb on + strand at 4262.377 kb on + strand at 4262.377 kb on + strand at 4262.412 kb on + strand at 4262.412 kb on + strand at 4262.417 kb on + strand at 4262.417 kb on + strand at 4263.097 kb on + strand at 4263.097 kb on + strand at 4263.097 kb on + strand at 4263.216 kb on - strand, within Pf6N2E2_3661 at 4263.216 kb on - strand, within Pf6N2E2_3661 at 4263.216 kb on - strand, within Pf6N2E2_3661 at 4263.493 kb on + strand, within Pf6N2E2_3661 at 4263.493 kb on + strand, within Pf6N2E2_3661 at 4263.494 kb on - strand, within Pf6N2E2_3661 at 4263.494 kb on - strand, within Pf6N2E2_3661 at 4263.494 kb on - strand, within Pf6N2E2_3661 at 4263.563 kb on - strand, within Pf6N2E2_3661 at 4263.601 kb on + strand, within Pf6N2E2_3661 at 4263.601 kb on + strand, within Pf6N2E2_3661 at 4263.601 kb on + strand, within Pf6N2E2_3661 at 4263.602 kb on - strand, within Pf6N2E2_3661 at 4263.608 kb on + strand, within Pf6N2E2_3661 at 4263.609 kb on - strand, within Pf6N2E2_3661 at 4263.609 kb on - strand, within Pf6N2E2_3661 at 4263.609 kb on - strand, within Pf6N2E2_3661 at 4263.702 kb on - strand, within Pf6N2E2_3661 at 4263.720 kb on - strand, within Pf6N2E2_3661 at 4263.806 kb on + strand, within Pf6N2E2_3661 at 4263.962 kb on + strand at 4263.963 kb on - strand at 4263.995 kb on + strand at 4263.998 kb on - strand at 4263.998 kb on - strand at 4264.426 kb on + strand at 4264.427 kb on - strand at 4264.427 kb on - strand at 4264.475 kb on - strand at 4264.475 kb on - strand at 4264.495 kb on + strand, within Pf6N2E2_3663 at 4264.496 kb on - strand, within Pf6N2E2_3663 at 4264.667 kb on + strand, within Pf6N2E2_3663 at 4264.668 kb on - strand, within Pf6N2E2_3663 at 4264.668 kb on - strand, within Pf6N2E2_3663 at 4264.669 kb on + strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.670 kb on - strand, within Pf6N2E2_3663 at 4264.768 kb on + strand, within Pf6N2E2_3663 at 4264.844 kb on - strand, within Pf6N2E2_3663 at 4264.941 kb on - strand, within Pf6N2E2_3663
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,262,144 - +7.2 4,262,144 - +7.5 4,262,144 - +7.5 4,262,147 + +6.7 4,262,147 + +7.3 4,262,147 + +8.0 4,262,148 - -0.9 4,262,148 - +8.2 4,262,148 - +7.2 4,262,148 - +4.9 4,262,148 - +7.7 4,262,148 - +7.7 4,262,190 + +7.1 4,262,190 + +8.1 4,262,191 - +7.5 4,262,191 - +6.7 4,262,252 + +6.7 4,262,264 - -2.6 4,262,279 + +6.7 4,262,279 + -1.3 4,262,295 - -3.0 4,262,376 - -1.6 4,262,377 + +7.2 4,262,377 + +7.1 4,262,377 + +3.9 4,262,412 + +6.6 4,262,412 + +5.5 4,262,417 + +7.2 4,262,417 + +7.3 4,263,097 + +1.8 4,263,097 + +2.3 4,263,097 + +1.8 4,263,216 - Pf6N2E2_3661 0.12 +7.3 4,263,216 - Pf6N2E2_3661 0.12 +7.7 4,263,216 - Pf6N2E2_3661 0.12 +6.1 4,263,493 + Pf6N2E2_3661 0.45 +8.3 4,263,493 + Pf6N2E2_3661 0.45 +6.3 4,263,494 - Pf6N2E2_3661 0.45 +6.3 4,263,494 - Pf6N2E2_3661 0.45 +4.7 4,263,494 - Pf6N2E2_3661 0.45 +4.0 4,263,563 - Pf6N2E2_3661 0.53 +7.0 4,263,601 + Pf6N2E2_3661 0.57 +6.6 4,263,601 + Pf6N2E2_3661 0.57 +6.6 4,263,601 + Pf6N2E2_3661 0.57 +6.2 4,263,602 - Pf6N2E2_3661 0.57 +8.0 4,263,608 + Pf6N2E2_3661 0.58 +5.5 4,263,609 - Pf6N2E2_3661 0.58 +7.5 4,263,609 - Pf6N2E2_3661 0.58 +5.9 4,263,609 - Pf6N2E2_3661 0.58 +6.4 4,263,702 - Pf6N2E2_3661 0.69 +6.6 4,263,720 - Pf6N2E2_3661 0.71 +6.6 4,263,806 + Pf6N2E2_3661 0.81 +6.9 4,263,962 + +5.8 4,263,963 - -0.1 4,263,995 + +6.3 4,263,998 - +6.7 4,263,998 - +6.7 4,264,426 + +7.9 4,264,427 - +7.3 4,264,427 - +7.6 4,264,475 - +6.8 4,264,475 - +7.5 4,264,495 + Pf6N2E2_3663 0.11 +6.0 4,264,496 - Pf6N2E2_3663 0.12 +7.3 4,264,667 + Pf6N2E2_3663 0.30 +6.9 4,264,668 - Pf6N2E2_3663 0.31 +7.1 4,264,668 - Pf6N2E2_3663 0.31 +7.0 4,264,669 + Pf6N2E2_3663 0.31 +7.3 4,264,670 - Pf6N2E2_3663 0.31 +6.4 4,264,670 - Pf6N2E2_3663 0.31 +6.5 4,264,670 - Pf6N2E2_3663 0.31 +7.5 4,264,670 - Pf6N2E2_3663 0.31 +7.2 4,264,670 - Pf6N2E2_3663 0.31 +7.4 4,264,670 - Pf6N2E2_3663 0.31 +7.3 4,264,768 + Pf6N2E2_3663 0.42 +7.6 4,264,844 - Pf6N2E2_3663 0.50 +6.7 4,264,941 - Pf6N2E2_3663 0.61 +7.0
Or see this region's nucleotide sequence