Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3435 and Pf6N2E2_3436 overlap by 4 nucleotides Pf6N2E2_3436 and Pf6N2E2_3437 overlap by 4 nucleotides
Pf6N2E2_3435 - hypothetical protein, at 3,995,528 to 4,000,123
_3435
Pf6N2E2_3436 - Putative toxin subunit, at 4,000,120 to 4,004,607
_3436
Pf6N2E2_3437 - Putative insecticidal toxin complex, at 4,004,604 to 4,007,393
_3437
Position (kb)
4000
4001
4002
4003
4004
4005 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3999.326 kb on + strand, within Pf6N2E2_3435 at 3999.327 kb on - strand, within Pf6N2E2_3435 at 3999.350 kb on - strand, within Pf6N2E2_3435 at 3999.365 kb on + strand, within Pf6N2E2_3435 at 3999.399 kb on - strand, within Pf6N2E2_3435 at 3999.458 kb on + strand, within Pf6N2E2_3435 at 3999.515 kb on + strand, within Pf6N2E2_3435 at 3999.515 kb on + strand, within Pf6N2E2_3435 at 3999.515 kb on + strand, within Pf6N2E2_3435 at 3999.576 kb on - strand, within Pf6N2E2_3435 at 3999.576 kb on - strand, within Pf6N2E2_3435 at 3999.576 kb on - strand, within Pf6N2E2_3435 at 3999.661 kb on + strand, within Pf6N2E2_3435 at 3999.746 kb on + strand at 3999.747 kb on - strand at 3999.764 kb on + strand at 3999.765 kb on - strand at 4000.091 kb on + strand at 4000.234 kb on + strand at 4000.388 kb on - strand at 4000.388 kb on - strand at 4000.388 kb on - strand at 4000.389 kb on + strand at 4000.484 kb on - strand at 4000.510 kb on + strand at 4000.510 kb on + strand at 4000.525 kb on + strand at 4000.564 kb on + strand at 4000.564 kb on + strand at 4000.604 kb on + strand, within Pf6N2E2_3436 at 4000.604 kb on + strand, within Pf6N2E2_3436 at 4000.604 kb on + strand, within Pf6N2E2_3436 at 4000.605 kb on - strand, within Pf6N2E2_3436 at 4000.605 kb on - strand, within Pf6N2E2_3436 at 4000.605 kb on - strand, within Pf6N2E2_3436 at 4000.631 kb on + strand, within Pf6N2E2_3436 at 4000.744 kb on + strand, within Pf6N2E2_3436 at 4000.745 kb on - strand, within Pf6N2E2_3436 at 4000.789 kb on + strand, within Pf6N2E2_3436 at 4000.819 kb on + strand, within Pf6N2E2_3436 at 4000.900 kb on + strand, within Pf6N2E2_3436 at 4000.900 kb on + strand, within Pf6N2E2_3436 at 4000.918 kb on + strand, within Pf6N2E2_3436 at 4000.918 kb on + strand, within Pf6N2E2_3436 at 4000.918 kb on + strand, within Pf6N2E2_3436 at 4000.918 kb on + strand, within Pf6N2E2_3436 at 4000.918 kb on + strand, within Pf6N2E2_3436 at 4000.918 kb on + strand, within Pf6N2E2_3436 at 4000.918 kb on + strand, within Pf6N2E2_3436 at 4001.009 kb on + strand, within Pf6N2E2_3436 at 4001.010 kb on - strand, within Pf6N2E2_3436 at 4001.013 kb on - strand, within Pf6N2E2_3436 at 4001.013 kb on - strand, within Pf6N2E2_3436 at 4001.013 kb on - strand, within Pf6N2E2_3436 at 4001.304 kb on - strand, within Pf6N2E2_3436 at 4001.329 kb on + strand, within Pf6N2E2_3436 at 4001.329 kb on + strand, within Pf6N2E2_3436 at 4001.329 kb on + strand, within Pf6N2E2_3436 at 4001.330 kb on - strand, within Pf6N2E2_3436 at 4001.332 kb on + strand, within Pf6N2E2_3436 at 4001.659 kb on + strand, within Pf6N2E2_3436 at 4001.702 kb on + strand, within Pf6N2E2_3436 at 4001.805 kb on - strand, within Pf6N2E2_3436 at 4001.897 kb on - strand, within Pf6N2E2_3436 at 4001.965 kb on + strand, within Pf6N2E2_3436 at 4001.965 kb on + strand, within Pf6N2E2_3436 at 4001.966 kb on - strand, within Pf6N2E2_3436 at 4002.059 kb on - strand, within Pf6N2E2_3436 at 4002.059 kb on - strand, within Pf6N2E2_3436 at 4002.059 kb on - strand, within Pf6N2E2_3436 at 4002.182 kb on - strand, within Pf6N2E2_3436 at 4002.185 kb on + strand, within Pf6N2E2_3436 at 4002.186 kb on - strand, within Pf6N2E2_3436 at 4002.207 kb on + strand, within Pf6N2E2_3436 at 4002.207 kb on + strand, within Pf6N2E2_3436 at 4002.208 kb on - strand, within Pf6N2E2_3436 at 4002.226 kb on - strand, within Pf6N2E2_3436 at 4002.388 kb on + strand, within Pf6N2E2_3436 at 4002.389 kb on - strand, within Pf6N2E2_3436 at 4002.400 kb on + strand, within Pf6N2E2_3436 at 4002.403 kb on + strand, within Pf6N2E2_3436 at 4002.404 kb on - strand, within Pf6N2E2_3436 at 4002.404 kb on - strand, within Pf6N2E2_3436 at 4002.616 kb on + strand, within Pf6N2E2_3436 at 4002.617 kb on - strand, within Pf6N2E2_3436 at 4002.704 kb on + strand, within Pf6N2E2_3436 at 4002.705 kb on - strand, within Pf6N2E2_3436 at 4002.705 kb on - strand, within Pf6N2E2_3436 at 4002.720 kb on - strand, within Pf6N2E2_3436 at 4002.757 kb on + strand, within Pf6N2E2_3436 at 4002.859 kb on + strand, within Pf6N2E2_3436 at 4002.865 kb on + strand, within Pf6N2E2_3436 at 4002.866 kb on - strand, within Pf6N2E2_3436 at 4002.866 kb on - strand, within Pf6N2E2_3436 at 4002.916 kb on + strand, within Pf6N2E2_3436 at 4002.916 kb on + strand, within Pf6N2E2_3436 at 4002.917 kb on - strand, within Pf6N2E2_3436 at 4002.917 kb on - strand, within Pf6N2E2_3436 at 4002.948 kb on - strand, within Pf6N2E2_3436 at 4002.984 kb on + strand, within Pf6N2E2_3436 at 4003.057 kb on + strand, within Pf6N2E2_3436 at 4003.058 kb on - strand, within Pf6N2E2_3436 at 4003.081 kb on + strand, within Pf6N2E2_3436 at 4003.081 kb on + strand, within Pf6N2E2_3436 at 4003.082 kb on - strand, within Pf6N2E2_3436 at 4003.201 kb on + strand, within Pf6N2E2_3436 at 4003.201 kb on + strand, within Pf6N2E2_3436 at 4003.201 kb on + strand, within Pf6N2E2_3436 at 4003.202 kb on - strand, within Pf6N2E2_3436 at 4003.202 kb on - strand, within Pf6N2E2_3436 at 4003.202 kb on - strand, within Pf6N2E2_3436 at 4003.279 kb on + strand, within Pf6N2E2_3436 at 4003.279 kb on + strand, within Pf6N2E2_3436 at 4003.280 kb on - strand, within Pf6N2E2_3436 at 4003.523 kb on - strand, within Pf6N2E2_3436 at 4003.523 kb on - strand, within Pf6N2E2_3436 at 4003.523 kb on - strand, within Pf6N2E2_3436 at 4003.523 kb on - strand, within Pf6N2E2_3436 at 4003.543 kb on + strand, within Pf6N2E2_3436 at 4003.606 kb on + strand, within Pf6N2E2_3436 at 4003.606 kb on + strand, within Pf6N2E2_3436 at 4003.606 kb on + strand, within Pf6N2E2_3436 at 4003.607 kb on - strand, within Pf6N2E2_3436 at 4003.607 kb on - strand, within Pf6N2E2_3436 at 4003.607 kb on - strand, within Pf6N2E2_3436 at 4003.664 kb on - strand, within Pf6N2E2_3436 at 4003.748 kb on + strand, within Pf6N2E2_3436 at 4003.748 kb on + strand, within Pf6N2E2_3436 at 4003.748 kb on + strand, within Pf6N2E2_3436 at 4003.749 kb on - strand, within Pf6N2E2_3436 at 4003.749 kb on - strand, within Pf6N2E2_3436 at 4003.766 kb on - strand, within Pf6N2E2_3436 at 4003.784 kb on + strand, within Pf6N2E2_3436 at 4003.784 kb on + strand, within Pf6N2E2_3436 at 4003.784 kb on + strand, within Pf6N2E2_3436 at 4003.785 kb on - strand, within Pf6N2E2_3436 at 4003.785 kb on - strand, within Pf6N2E2_3436 at 4003.894 kb on + strand, within Pf6N2E2_3436 at 4003.894 kb on + strand, within Pf6N2E2_3436 at 4003.894 kb on + strand, within Pf6N2E2_3436 at 4003.894 kb on + strand, within Pf6N2E2_3436 at 4003.895 kb on - strand, within Pf6N2E2_3436 at 4003.895 kb on - strand, within Pf6N2E2_3436 at 4003.895 kb on - strand, within Pf6N2E2_3436 at 4004.001 kb on + strand, within Pf6N2E2_3436 at 4004.001 kb on + strand, within Pf6N2E2_3436 at 4004.002 kb on - strand, within Pf6N2E2_3436 at 4004.234 kb on - strand at 4004.329 kb on + strand at 4004.329 kb on + strand at 4004.330 kb on - strand at 4004.359 kb on + strand at 4004.369 kb on - strand at 4004.389 kb on + strand at 4004.389 kb on + strand at 4004.389 kb on + strand at 4004.440 kb on + strand at 4004.441 kb on - strand at 4004.441 kb on - strand at 4004.441 kb on - strand at 4004.498 kb on - strand at 4004.498 kb on - strand at 4004.498 kb on - strand at 4004.498 kb on - strand at 4004.498 kb on - strand at 4004.528 kb on - strand at 4004.528 kb on - strand at 4004.682 kb on + strand at 4004.818 kb on - strand at 4005.249 kb on + strand, within Pf6N2E2_3437 at 4005.352 kb on + strand, within Pf6N2E2_3437 at 4005.353 kb on - strand, within Pf6N2E2_3437 at 4005.362 kb on + strand, within Pf6N2E2_3437 at 4005.396 kb on + strand, within Pf6N2E2_3437
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 3,999,326 + Pf6N2E2_3435 0.83 -0.2 3,999,327 - Pf6N2E2_3435 0.83 +0.1 3,999,350 - Pf6N2E2_3435 0.83 +0.4 3,999,365 + Pf6N2E2_3435 0.83 -2.4 3,999,399 - Pf6N2E2_3435 0.84 -0.5 3,999,458 + Pf6N2E2_3435 0.86 +1.3 3,999,515 + Pf6N2E2_3435 0.87 -0.1 3,999,515 + Pf6N2E2_3435 0.87 +0.1 3,999,515 + Pf6N2E2_3435 0.87 +0.2 3,999,576 - Pf6N2E2_3435 0.88 -0.4 3,999,576 - Pf6N2E2_3435 0.88 +0.2 3,999,576 - Pf6N2E2_3435 0.88 -0.3 3,999,661 + Pf6N2E2_3435 0.90 -0.0 3,999,746 + +0.1 3,999,747 - -1.3 3,999,764 + +0.5 3,999,765 - -0.7 4,000,091 + +0.8 4,000,234 + +0.2 4,000,388 - +0.0 4,000,388 - +1.4 4,000,388 - -1.4 4,000,389 + -1.2 4,000,484 - -0.2 4,000,510 + +1.5 4,000,510 + -0.6 4,000,525 + -1.2 4,000,564 + -0.4 4,000,564 + +0.4 4,000,604 + Pf6N2E2_3436 0.11 +0.2 4,000,604 + Pf6N2E2_3436 0.11 -2.9 4,000,604 + Pf6N2E2_3436 0.11 -2.8 4,000,605 - Pf6N2E2_3436 0.11 +0.1 4,000,605 - Pf6N2E2_3436 0.11 -1.8 4,000,605 - Pf6N2E2_3436 0.11 +0.3 4,000,631 + Pf6N2E2_3436 0.11 -0.6 4,000,744 + Pf6N2E2_3436 0.14 -1.1 4,000,745 - Pf6N2E2_3436 0.14 -0.4 4,000,789 + Pf6N2E2_3436 0.15 -0.6 4,000,819 + Pf6N2E2_3436 0.16 +2.0 4,000,900 + Pf6N2E2_3436 0.17 +0.0 4,000,900 + Pf6N2E2_3436 0.17 -0.1 4,000,918 + Pf6N2E2_3436 0.18 -0.5 4,000,918 + Pf6N2E2_3436 0.18 -1.4 4,000,918 + Pf6N2E2_3436 0.18 -0.5 4,000,918 + Pf6N2E2_3436 0.18 -0.5 4,000,918 + Pf6N2E2_3436 0.18 -0.3 4,000,918 + Pf6N2E2_3436 0.18 +0.6 4,000,918 + Pf6N2E2_3436 0.18 -1.6 4,001,009 + Pf6N2E2_3436 0.20 -0.1 4,001,010 - Pf6N2E2_3436 0.20 -0.3 4,001,013 - Pf6N2E2_3436 0.20 -0.4 4,001,013 - Pf6N2E2_3436 0.20 -1.3 4,001,013 - Pf6N2E2_3436 0.20 +0.3 4,001,304 - Pf6N2E2_3436 0.26 -0.4 4,001,329 + Pf6N2E2_3436 0.27 -0.0 4,001,329 + Pf6N2E2_3436 0.27 +0.1 4,001,329 + Pf6N2E2_3436 0.27 -0.1 4,001,330 - Pf6N2E2_3436 0.27 -1.9 4,001,332 + Pf6N2E2_3436 0.27 -0.5 4,001,659 + Pf6N2E2_3436 0.34 -0.6 4,001,702 + Pf6N2E2_3436 0.35 +0.7 4,001,805 - Pf6N2E2_3436 0.38 -1.0 4,001,897 - Pf6N2E2_3436 0.40 +0.1 4,001,965 + Pf6N2E2_3436 0.41 +0.6 4,001,965 + Pf6N2E2_3436 0.41 -0.7 4,001,966 - Pf6N2E2_3436 0.41 -0.2 4,002,059 - Pf6N2E2_3436 0.43 -0.2 4,002,059 - Pf6N2E2_3436 0.43 -0.2 4,002,059 - Pf6N2E2_3436 0.43 +0.3 4,002,182 - Pf6N2E2_3436 0.46 -0.5 4,002,185 + Pf6N2E2_3436 0.46 +0.4 4,002,186 - Pf6N2E2_3436 0.46 +0.9 4,002,207 + Pf6N2E2_3436 0.47 +1.2 4,002,207 + Pf6N2E2_3436 0.47 +0.8 4,002,208 - Pf6N2E2_3436 0.47 -0.8 4,002,226 - Pf6N2E2_3436 0.47 +0.5 4,002,388 + Pf6N2E2_3436 0.51 -0.1 4,002,389 - Pf6N2E2_3436 0.51 -0.2 4,002,400 + Pf6N2E2_3436 0.51 +0.3 4,002,403 + Pf6N2E2_3436 0.51 -0.8 4,002,404 - Pf6N2E2_3436 0.51 +0.6 4,002,404 - Pf6N2E2_3436 0.51 -1.8 4,002,616 + Pf6N2E2_3436 0.56 +0.2 4,002,617 - Pf6N2E2_3436 0.56 +0.8 4,002,704 + Pf6N2E2_3436 0.58 -0.1 4,002,705 - Pf6N2E2_3436 0.58 +1.0 4,002,705 - Pf6N2E2_3436 0.58 -1.5 4,002,720 - Pf6N2E2_3436 0.58 -1.4 4,002,757 + Pf6N2E2_3436 0.59 +0.0 4,002,859 + Pf6N2E2_3436 0.61 -1.1 4,002,865 + Pf6N2E2_3436 0.61 -0.9 4,002,866 - Pf6N2E2_3436 0.61 -2.3 4,002,866 - Pf6N2E2_3436 0.61 -0.0 4,002,916 + Pf6N2E2_3436 0.62 -0.8 4,002,916 + Pf6N2E2_3436 0.62 +0.9 4,002,917 - Pf6N2E2_3436 0.62 +1.2 4,002,917 - Pf6N2E2_3436 0.62 -0.4 4,002,948 - Pf6N2E2_3436 0.63 +0.9 4,002,984 + Pf6N2E2_3436 0.64 -0.1 4,003,057 + Pf6N2E2_3436 0.65 -1.2 4,003,058 - Pf6N2E2_3436 0.65 -0.4 4,003,081 + Pf6N2E2_3436 0.66 +0.5 4,003,081 + Pf6N2E2_3436 0.66 -0.9 4,003,082 - Pf6N2E2_3436 0.66 -2.1 4,003,201 + Pf6N2E2_3436 0.69 -1.5 4,003,201 + Pf6N2E2_3436 0.69 -0.6 4,003,201 + Pf6N2E2_3436 0.69 -0.1 4,003,202 - Pf6N2E2_3436 0.69 -0.6 4,003,202 - Pf6N2E2_3436 0.69 -0.3 4,003,202 - Pf6N2E2_3436 0.69 +0.4 4,003,279 + Pf6N2E2_3436 0.70 -0.2 4,003,279 + Pf6N2E2_3436 0.70 +0.5 4,003,280 - Pf6N2E2_3436 0.70 -0.2 4,003,523 - Pf6N2E2_3436 0.76 -1.1 4,003,523 - Pf6N2E2_3436 0.76 -0.3 4,003,523 - Pf6N2E2_3436 0.76 +0.7 4,003,523 - Pf6N2E2_3436 0.76 -1.0 4,003,543 + Pf6N2E2_3436 0.76 -0.7 4,003,606 + Pf6N2E2_3436 0.78 +0.8 4,003,606 + Pf6N2E2_3436 0.78 +0.3 4,003,606 + Pf6N2E2_3436 0.78 +0.3 4,003,607 - Pf6N2E2_3436 0.78 -0.7 4,003,607 - Pf6N2E2_3436 0.78 -0.6 4,003,607 - Pf6N2E2_3436 0.78 +0.7 4,003,664 - Pf6N2E2_3436 0.79 +0.5 4,003,748 + Pf6N2E2_3436 0.81 -0.6 4,003,748 + Pf6N2E2_3436 0.81 +0.4 4,003,748 + Pf6N2E2_3436 0.81 +0.5 4,003,749 - Pf6N2E2_3436 0.81 -1.1 4,003,749 - Pf6N2E2_3436 0.81 +0.0 4,003,766 - Pf6N2E2_3436 0.81 +0.3 4,003,784 + Pf6N2E2_3436 0.82 -1.5 4,003,784 + Pf6N2E2_3436 0.82 -0.1 4,003,784 + Pf6N2E2_3436 0.82 +0.2 4,003,785 - Pf6N2E2_3436 0.82 -1.0 4,003,785 - Pf6N2E2_3436 0.82 -1.0 4,003,894 + Pf6N2E2_3436 0.84 -0.4 4,003,894 + Pf6N2E2_3436 0.84 -0.6 4,003,894 + Pf6N2E2_3436 0.84 -0.6 4,003,894 + Pf6N2E2_3436 0.84 +1.1 4,003,895 - Pf6N2E2_3436 0.84 -0.9 4,003,895 - Pf6N2E2_3436 0.84 +0.3 4,003,895 - Pf6N2E2_3436 0.84 +0.6 4,004,001 + Pf6N2E2_3436 0.86 -1.6 4,004,001 + Pf6N2E2_3436 0.86 +0.3 4,004,002 - Pf6N2E2_3436 0.86 -1.0 4,004,234 - +0.0 4,004,329 + -2.5 4,004,329 + -0.1 4,004,330 - -0.9 4,004,359 + +0.2 4,004,369 - -0.2 4,004,389 + -1.1 4,004,389 + -0.7 4,004,389 + +0.4 4,004,440 + -1.0 4,004,441 - +0.1 4,004,441 - -0.7 4,004,441 - +0.7 4,004,498 - +1.0 4,004,498 - +1.0 4,004,498 - -0.6 4,004,498 - -0.3 4,004,498 - -0.4 4,004,528 - +1.1 4,004,528 - +0.2 4,004,682 + +0.0 4,004,818 - -0.3 4,005,249 + Pf6N2E2_3437 0.23 +0.0 4,005,352 + Pf6N2E2_3437 0.27 -0.5 4,005,353 - Pf6N2E2_3437 0.27 +0.5 4,005,362 + Pf6N2E2_3437 0.27 -0.2 4,005,396 + Pf6N2E2_3437 0.28 +0.3
Or see this region's nucleotide sequence