Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3336 and Pf6N2E2_3337 are separated by 314 nucleotides Pf6N2E2_3337 and Pf6N2E2_3338 are separated by 28 nucleotides Pf6N2E2_3338 and Pf6N2E2_3339 overlap by 1 nucleotides
Pf6N2E2_3336 - Phosphodiesterase/alkaline phosphatase D, at 3,858,861 to 3,860,402
_3336
Pf6N2E2_3337 - PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69), at 3,860,717 to 3,862,444
_3337
Pf6N2E2_3338 - 1-phosphofructokinase (EC 2.7.1.56), at 3,862,473 to 3,863,414
_3338
Pf6N2E2_3339 - Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9), at 3,863,414 to 3,866,278
_3339
Position (kb)
3860
3861
3862
3863 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3859.735 kb on + strand, within Pf6N2E2_3336 at 3859.736 kb on - strand, within Pf6N2E2_3336 at 3859.742 kb on - strand, within Pf6N2E2_3336 at 3859.858 kb on + strand, within Pf6N2E2_3336 at 3859.858 kb on + strand, within Pf6N2E2_3336 at 3859.858 kb on + strand, within Pf6N2E2_3336 at 3859.859 kb on - strand, within Pf6N2E2_3336 at 3859.859 kb on - strand, within Pf6N2E2_3336 at 3859.859 kb on - strand, within Pf6N2E2_3336 at 3859.915 kb on + strand, within Pf6N2E2_3336 at 3859.915 kb on + strand, within Pf6N2E2_3336 at 3859.916 kb on - strand, within Pf6N2E2_3336 at 3859.916 kb on - strand, within Pf6N2E2_3336 at 3859.916 kb on - strand, within Pf6N2E2_3336 at 3859.916 kb on - strand, within Pf6N2E2_3336 at 3860.246 kb on + strand, within Pf6N2E2_3336 at 3860.246 kb on + strand, within Pf6N2E2_3336 at 3860.246 kb on + strand, within Pf6N2E2_3336 at 3860.246 kb on + strand, within Pf6N2E2_3336 at 3860.430 kb on + strand at 3860.430 kb on + strand at 3860.431 kb on - strand at 3860.431 kb on - strand at 3860.431 kb on - strand at 3860.489 kb on + strand at 3860.490 kb on - strand at 3860.490 kb on - strand at 3860.490 kb on - strand at 3860.490 kb on - strand at 3860.610 kb on + strand at 3860.683 kb on + strand at 3860.683 kb on + strand at 3860.684 kb on - strand at 3860.684 kb on - strand at 3860.747 kb on + strand at 3860.820 kb on + strand at 3860.820 kb on + strand at 3860.821 kb on - strand at 3860.821 kb on - strand at 3860.821 kb on - strand at 3860.821 kb on - strand at 3860.821 kb on - strand at 3860.901 kb on + strand, within Pf6N2E2_3337 at 3861.321 kb on + strand, within Pf6N2E2_3337 at 3861.321 kb on + strand, within Pf6N2E2_3337 at 3861.321 kb on + strand, within Pf6N2E2_3337 at 3861.321 kb on + strand, within Pf6N2E2_3337 at 3861.321 kb on + strand, within Pf6N2E2_3337 at 3861.322 kb on - strand, within Pf6N2E2_3337 at 3861.322 kb on - strand, within Pf6N2E2_3337 at 3861.322 kb on - strand, within Pf6N2E2_3337 at 3861.322 kb on - strand, within Pf6N2E2_3337 at 3861.322 kb on - strand, within Pf6N2E2_3337 at 3861.546 kb on + strand, within Pf6N2E2_3337 at 3861.546 kb on + strand, within Pf6N2E2_3337 at 3861.555 kb on + strand, within Pf6N2E2_3337 at 3861.555 kb on + strand, within Pf6N2E2_3337 at 3861.556 kb on - strand, within Pf6N2E2_3337 at 3861.738 kb on + strand, within Pf6N2E2_3337 at 3861.738 kb on + strand, within Pf6N2E2_3337 at 3861.739 kb on - strand, within Pf6N2E2_3337 at 3861.868 kb on - strand, within Pf6N2E2_3337 at 3862.451 kb on + strand at 3862.452 kb on - strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.474 kb on + strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3862.475 kb on - strand at 3863.168 kb on + strand, within Pf6N2E2_3338 at 3863.168 kb on + strand, within Pf6N2E2_3338 at 3863.168 kb on + strand, within Pf6N2E2_3338 at 3863.415 kb on + strand at 3863.429 kb on + strand at 3863.430 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 3,859,735 + Pf6N2E2_3336 0.57 -2.5 3,859,736 - Pf6N2E2_3336 0.57 -0.2 3,859,742 - Pf6N2E2_3336 0.57 -0.1 3,859,858 + Pf6N2E2_3336 0.65 -1.0 3,859,858 + Pf6N2E2_3336 0.65 -0.6 3,859,858 + Pf6N2E2_3336 0.65 +0.1 3,859,859 - Pf6N2E2_3336 0.65 -0.1 3,859,859 - Pf6N2E2_3336 0.65 -0.5 3,859,859 - Pf6N2E2_3336 0.65 -1.7 3,859,915 + Pf6N2E2_3336 0.68 -0.0 3,859,915 + Pf6N2E2_3336 0.68 -0.9 3,859,916 - Pf6N2E2_3336 0.68 +0.3 3,859,916 - Pf6N2E2_3336 0.68 -0.0 3,859,916 - Pf6N2E2_3336 0.68 -0.2 3,859,916 - Pf6N2E2_3336 0.68 +0.6 3,860,246 + Pf6N2E2_3336 0.90 +0.1 3,860,246 + Pf6N2E2_3336 0.90 -0.8 3,860,246 + Pf6N2E2_3336 0.90 -0.8 3,860,246 + Pf6N2E2_3336 0.90 +0.6 3,860,430 + +0.8 3,860,430 + -0.8 3,860,431 - -1.1 3,860,431 - -0.1 3,860,431 - -0.4 3,860,489 + -0.8 3,860,490 - -0.8 3,860,490 - +0.1 3,860,490 - -0.3 3,860,490 - +0.5 3,860,610 + -0.0 3,860,683 + +0.6 3,860,683 + +0.5 3,860,684 - -2.1 3,860,684 - +0.6 3,860,747 + -0.5 3,860,820 + -0.0 3,860,820 + -0.2 3,860,821 - -1.9 3,860,821 - -1.1 3,860,821 - -1.2 3,860,821 - -0.8 3,860,821 - -3.7 3,860,901 + Pf6N2E2_3337 0.11 -1.1 3,861,321 + Pf6N2E2_3337 0.35 -1.0 3,861,321 + Pf6N2E2_3337 0.35 -0.7 3,861,321 + Pf6N2E2_3337 0.35 -0.3 3,861,321 + Pf6N2E2_3337 0.35 -1.7 3,861,321 + Pf6N2E2_3337 0.35 -0.9 3,861,322 - Pf6N2E2_3337 0.35 -2.8 3,861,322 - Pf6N2E2_3337 0.35 -0.4 3,861,322 - Pf6N2E2_3337 0.35 -1.8 3,861,322 - Pf6N2E2_3337 0.35 -0.5 3,861,322 - Pf6N2E2_3337 0.35 -0.1 3,861,546 + Pf6N2E2_3337 0.48 -2.8 3,861,546 + Pf6N2E2_3337 0.48 -1.2 3,861,555 + Pf6N2E2_3337 0.48 -1.6 3,861,555 + Pf6N2E2_3337 0.48 -0.6 3,861,556 - Pf6N2E2_3337 0.49 -0.2 3,861,738 + Pf6N2E2_3337 0.59 -0.7 3,861,738 + Pf6N2E2_3337 0.59 -2.2 3,861,739 - Pf6N2E2_3337 0.59 -2.0 3,861,868 - Pf6N2E2_3337 0.67 -0.9 3,862,451 + -0.0 3,862,452 - -1.1 3,862,474 + -1.7 3,862,474 + -0.1 3,862,474 + +0.1 3,862,474 + -1.0 3,862,474 + -1.3 3,862,474 + +0.1 3,862,474 + -0.1 3,862,474 + +1.0 3,862,475 - -0.1 3,862,475 - -0.2 3,862,475 - -0.9 3,862,475 - +0.1 3,862,475 - -0.0 3,862,475 - -1.1 3,862,475 - +1.0 3,862,475 - -0.4 3,862,475 - -0.4 3,863,168 + Pf6N2E2_3338 0.74 +1.7 3,863,168 + Pf6N2E2_3338 0.74 +2.1 3,863,168 + Pf6N2E2_3338 0.74 +0.1 3,863,415 + +1.5 3,863,429 + -3.1 3,863,430 - +0.5
Or see this region's nucleotide sequence