Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3264 and Pf6N2E2_3265 overlap by 14 nucleotides Pf6N2E2_3265 and Pf6N2E2_3266 overlap by 1 nucleotides Pf6N2E2_3266 and Pf6N2E2_3267 are separated by 188 nucleotides
Pf6N2E2_3264 - Uncharacterized protein YrbK clustered with lipopolysaccharide transporters, at 3,771,281 to 3,771,850
_3264
Pf6N2E2_3265 - LptA, protein essential for LPS transport across the periplasm, at 3,771,837 to 3,772,388
_3265
Pf6N2E2_3266 - Lipopolysaccharide ABC transporter, ATP-binding protein LptB, at 3,772,388 to 3,773,113
_3266
Pf6N2E2_3267 - RNA polymerase sigma-54 factor RpoN, at 3,773,302 to 3,774,771
_3267
Position (kb)
3772
3773
3774 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3771.716 kb on + strand, within Pf6N2E2_3264 at 3771.716 kb on + strand, within Pf6N2E2_3264 at 3771.716 kb on + strand, within Pf6N2E2_3264 at 3771.716 kb on + strand, within Pf6N2E2_3264 at 3771.825 kb on + strand at 3773.111 kb on + strand at 3773.111 kb on + strand at 3773.112 kb on - strand at 3773.112 kb on - strand at 3773.149 kb on - strand at 3773.155 kb on - strand at 3773.170 kb on + strand at 3773.170 kb on + strand at 3773.171 kb on - strand at 3773.197 kb on + strand at 3773.230 kb on + strand at 3773.230 kb on + strand at 3773.258 kb on + strand at 3773.258 kb on + strand at 3773.266 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 3,771,716 + Pf6N2E2_3264 0.76 -2.8 3,771,716 + Pf6N2E2_3264 0.76 -2.5 3,771,716 + Pf6N2E2_3264 0.76 -2.6 3,771,716 + Pf6N2E2_3264 0.76 -4.0 3,771,825 + -0.8 3,773,111 + +0.0 3,773,111 + -0.1 3,773,112 - +1.1 3,773,112 - -0.9 3,773,149 - -1.1 3,773,155 - -3.3 3,773,170 + -1.0 3,773,170 + -1.3 3,773,171 - -2.4 3,773,197 + -0.1 3,773,230 + -1.4 3,773,230 + +0.6 3,773,258 + +1.2 3,773,258 + +0.2 3,773,266 + -0.1
Or see this region's nucleotide sequence