Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_308 and Pf6N2E2_309 are separated by 219 nucleotides Pf6N2E2_309 and Pf6N2E2_310 are separated by 2 nucleotides Pf6N2E2_310 and Pf6N2E2_311 are separated by 141 nucleotides Pf6N2E2_311 and Pf6N2E2_312 are separated by 80 nucleotides Pf6N2E2_312 and Pf6N2E2_313 are separated by 164 nucleotides
Pf6N2E2_308 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusD, at 376,989 to 377,381
_308
Pf6N2E2_309 - FIG00957702: hypothetical protein, at 377,601 to 377,930
_309
Pf6N2E2_310 - hypothetical protein, at 377,933 to 378,193
_310
Pf6N2E2_311 - hypothetical protein, at 378,335 to 378,685
_311
Pf6N2E2_312 - Histone acetyltransferase HPA2 and related acetyltransferases, at 378,766 to 379,197
_312
Pf6N2E2_313 - Gamma-glutamyltranspeptidase (EC 2.3.2.2), at 379,362 to 381,089
_313
Position (kb) 378
379 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 377.602 kb on + strand at 377.603 kb on - strand at 377.629 kb on + strand at 377.696 kb on - strand, within Pf6N2E2_309 at 377.696 kb on - strand, within Pf6N2E2_309 at 378.012 kb on + strand, within Pf6N2E2_310 at 378.012 kb on + strand, within Pf6N2E2_310 at 378.012 kb on + strand, within Pf6N2E2_310 at 378.012 kb on + strand, within Pf6N2E2_310 at 378.012 kb on + strand, within Pf6N2E2_310 at 378.013 kb on - strand, within Pf6N2E2_310 at 378.013 kb on - strand, within Pf6N2E2_310 at 378.013 kb on - strand, within Pf6N2E2_310 at 378.069 kb on + strand, within Pf6N2E2_310 at 378.070 kb on - strand, within Pf6N2E2_310 at 378.154 kb on - strand, within Pf6N2E2_310 at 378.171 kb on + strand at 378.172 kb on - strand at 378.172 kb on - strand at 378.302 kb on + strand at 378.422 kb on + strand, within Pf6N2E2_311 at 378.423 kb on - strand, within Pf6N2E2_311 at 378.423 kb on - strand, within Pf6N2E2_311 at 378.423 kb on - strand, within Pf6N2E2_311 at 378.423 kb on - strand, within Pf6N2E2_311 at 378.558 kb on - strand, within Pf6N2E2_311 at 378.558 kb on - strand, within Pf6N2E2_311 at 378.691 kb on + strand at 378.691 kb on + strand at 378.691 kb on + strand at 378.691 kb on + strand at 378.692 kb on - strand at 378.733 kb on + strand at 378.733 kb on + strand at 378.768 kb on - strand at 378.863 kb on + strand, within Pf6N2E2_312 at 379.007 kb on + strand, within Pf6N2E2_312 at 379.007 kb on + strand, within Pf6N2E2_312 at 379.007 kb on + strand, within Pf6N2E2_312 at 379.007 kb on + strand, within Pf6N2E2_312 at 379.008 kb on - strand, within Pf6N2E2_312 at 379.008 kb on - strand, within Pf6N2E2_312 at 379.008 kb on - strand, within Pf6N2E2_312 at 379.120 kb on - strand, within Pf6N2E2_312 at 379.120 kb on - strand, within Pf6N2E2_312 at 379.121 kb on + strand, within Pf6N2E2_312 at 379.121 kb on + strand, within Pf6N2E2_312 at 379.122 kb on - strand, within Pf6N2E2_312 at 379.189 kb on + strand at 379.210 kb on + strand at 379.290 kb on - strand at 379.308 kb on + strand at 379.308 kb on + strand at 379.308 kb on + strand at 379.308 kb on + strand at 379.308 kb on + strand at 379.308 kb on + strand at 379.308 kb on + strand at 379.308 kb on + strand at 379.308 kb on + strand at 379.309 kb on - strand at 379.309 kb on - strand at 379.309 kb on - strand at 379.309 kb on - strand at 379.309 kb on - strand at 379.309 kb on - strand at 379.309 kb on - strand at 379.309 kb on - strand at 379.309 kb on - strand at 379.309 kb on - strand at 379.312 kb on + strand at 379.312 kb on + strand at 379.313 kb on - strand at 379.313 kb on - strand at 379.313 kb on - strand at 379.422 kb on + strand at 379.422 kb on + strand at 379.422 kb on + strand at 379.422 kb on + strand at 379.422 kb on + strand at 379.423 kb on - strand at 379.423 kb on - strand at 379.423 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 377,602 + +0.4 377,603 - -3.1 377,629 + -0.1 377,696 - Pf6N2E2_309 0.29 +0.9 377,696 - Pf6N2E2_309 0.29 +0.1 378,012 + Pf6N2E2_310 0.30 -0.5 378,012 + Pf6N2E2_310 0.30 -3.1 378,012 + Pf6N2E2_310 0.30 -2.2 378,012 + Pf6N2E2_310 0.30 -0.5 378,012 + Pf6N2E2_310 0.30 +0.5 378,013 - Pf6N2E2_310 0.31 -0.4 378,013 - Pf6N2E2_310 0.31 +0.6 378,013 - Pf6N2E2_310 0.31 +0.2 378,069 + Pf6N2E2_310 0.52 -0.4 378,070 - Pf6N2E2_310 0.52 -0.1 378,154 - Pf6N2E2_310 0.85 +0.4 378,171 + -0.4 378,172 - -0.5 378,172 - -0.1 378,302 + -0.4 378,422 + Pf6N2E2_311 0.25 -1.3 378,423 - Pf6N2E2_311 0.25 -3.6 378,423 - Pf6N2E2_311 0.25 -2.7 378,423 - Pf6N2E2_311 0.25 -1.1 378,423 - Pf6N2E2_311 0.25 -1.2 378,558 - Pf6N2E2_311 0.64 -0.2 378,558 - Pf6N2E2_311 0.64 -1.4 378,691 + +0.3 378,691 + +0.7 378,691 + +0.3 378,691 + -0.5 378,692 - -0.0 378,733 + +1.8 378,733 + +0.4 378,768 - +1.1 378,863 + Pf6N2E2_312 0.22 -0.4 379,007 + Pf6N2E2_312 0.56 +0.1 379,007 + Pf6N2E2_312 0.56 +0.4 379,007 + Pf6N2E2_312 0.56 -0.5 379,007 + Pf6N2E2_312 0.56 -0.2 379,008 - Pf6N2E2_312 0.56 -0.3 379,008 - Pf6N2E2_312 0.56 -1.0 379,008 - Pf6N2E2_312 0.56 -0.8 379,120 - Pf6N2E2_312 0.82 -0.1 379,120 - Pf6N2E2_312 0.82 -0.1 379,121 + Pf6N2E2_312 0.82 +0.4 379,121 + Pf6N2E2_312 0.82 -0.4 379,122 - Pf6N2E2_312 0.82 -1.4 379,189 + -2.5 379,210 + -1.3 379,290 - +2.0 379,308 + -0.7 379,308 + +0.0 379,308 + -0.8 379,308 + -0.7 379,308 + -0.0 379,308 + +0.1 379,308 + -1.2 379,308 + -0.2 379,308 + +0.0 379,309 - +0.2 379,309 - -0.1 379,309 - +0.9 379,309 - +0.3 379,309 - -0.3 379,309 - -0.0 379,309 - -1.1 379,309 - -2.3 379,309 - -0.9 379,309 - -0.8 379,312 + -0.7 379,312 + +0.1 379,313 - +0.4 379,313 - -1.4 379,313 - +0.7 379,422 + -0.3 379,422 + -0.5 379,422 + +0.8 379,422 + +0.9 379,422 + +0.0 379,423 - +0.5 379,423 - +0.6 379,423 - -0.3
Or see this region's nucleotide sequence