Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3093 and Pf6N2E2_3094 are separated by 71 nucleotides Pf6N2E2_3094 and Pf6N2E2_3095 overlap by 4 nucleotides Pf6N2E2_3095 and Pf6N2E2_3096 overlap by 22 nucleotides Pf6N2E2_3096 and Pf6N2E2_3097 are separated by 1 nucleotides
Pf6N2E2_3093 - Transcriptional regulator, TetR family, at 3,581,921 to 3,582,556
_3093
Pf6N2E2_3094 - SAM-dependent methyltransferase (EC 2.1.1.-), at 3,582,628 to 3,583,410
_3094
Pf6N2E2_3095 - hypothetical protein, at 3,583,407 to 3,583,523
_3095
Pf6N2E2_3096 - Periplasmic protein TonB, links inner and outer membranes, at 3,583,502 to 3,584,122
_3096
Pf6N2E2_3097 - extensin-like protein, at 3,584,124 to 3,584,822
_3097
Position (kb)
3583
3584 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3582.513 kb on + strand at 3582.513 kb on + strand at 3582.513 kb on + strand at 3582.513 kb on + strand at 3582.513 kb on + strand at 3582.514 kb on - strand at 3582.514 kb on - strand at 3582.514 kb on - strand at 3582.554 kb on + strand at 3582.554 kb on + strand at 3582.555 kb on - strand at 3582.653 kb on + strand at 3582.662 kb on + strand at 3582.711 kb on + strand, within Pf6N2E2_3094 at 3582.711 kb on + strand, within Pf6N2E2_3094 at 3582.711 kb on + strand, within Pf6N2E2_3094 at 3582.711 kb on + strand, within Pf6N2E2_3094 at 3582.711 kb on + strand, within Pf6N2E2_3094 at 3582.711 kb on + strand, within Pf6N2E2_3094 at 3582.712 kb on - strand, within Pf6N2E2_3094 at 3582.712 kb on - strand, within Pf6N2E2_3094 at 3582.712 kb on - strand, within Pf6N2E2_3094 at 3582.881 kb on + strand, within Pf6N2E2_3094 at 3582.881 kb on + strand, within Pf6N2E2_3094 at 3582.882 kb on - strand, within Pf6N2E2_3094 at 3583.178 kb on + strand, within Pf6N2E2_3094 at 3583.178 kb on + strand, within Pf6N2E2_3094 at 3583.178 kb on + strand, within Pf6N2E2_3094 at 3583.179 kb on - strand, within Pf6N2E2_3094 at 3583.431 kb on - strand, within Pf6N2E2_3095 at 3583.553 kb on + strand at 3583.553 kb on + strand at 3583.554 kb on - strand at 3583.554 kb on - strand at 3583.665 kb on - strand, within Pf6N2E2_3096 at 3584.185 kb on + strand at 3584.186 kb on - strand at 3584.186 kb on - strand at 3584.186 kb on - strand at 3584.186 kb on - strand at 3584.214 kb on - strand, within Pf6N2E2_3097 at 3584.214 kb on - strand, within Pf6N2E2_3097 at 3584.214 kb on - strand, within Pf6N2E2_3097 at 3584.377 kb on + strand, within Pf6N2E2_3097 at 3584.377 kb on + strand, within Pf6N2E2_3097 at 3584.377 kb on + strand, within Pf6N2E2_3097 at 3584.377 kb on + strand, within Pf6N2E2_3097 at 3584.378 kb on - strand, within Pf6N2E2_3097 at 3584.378 kb on - strand, within Pf6N2E2_3097 at 3584.378 kb on - strand, within Pf6N2E2_3097 at 3584.378 kb on - strand, within Pf6N2E2_3097 at 3584.378 kb on - strand, within Pf6N2E2_3097 at 3584.378 kb on - strand, within Pf6N2E2_3097 at 3584.378 kb on - strand, within Pf6N2E2_3097 at 3584.378 kb on - strand, within Pf6N2E2_3097 at 3584.434 kb on + strand, within Pf6N2E2_3097 at 3584.434 kb on + strand, within Pf6N2E2_3097 at 3584.434 kb on + strand, within Pf6N2E2_3097 at 3584.435 kb on - strand, within Pf6N2E2_3097 at 3584.435 kb on - strand, within Pf6N2E2_3097
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 3,582,513 + +1.3 3,582,513 + -0.8 3,582,513 + +0.1 3,582,513 + +0.0 3,582,513 + -0.3 3,582,514 - -0.6 3,582,514 - +0.4 3,582,514 - -0.8 3,582,554 + +0.6 3,582,554 + -0.1 3,582,555 - -3.1 3,582,653 + +0.6 3,582,662 + +0.9 3,582,711 + Pf6N2E2_3094 0.11 -0.1 3,582,711 + Pf6N2E2_3094 0.11 +1.2 3,582,711 + Pf6N2E2_3094 0.11 -1.1 3,582,711 + Pf6N2E2_3094 0.11 -1.1 3,582,711 + Pf6N2E2_3094 0.11 -1.6 3,582,711 + Pf6N2E2_3094 0.11 -1.2 3,582,712 - Pf6N2E2_3094 0.11 -1.5 3,582,712 - Pf6N2E2_3094 0.11 -0.9 3,582,712 - Pf6N2E2_3094 0.11 +0.3 3,582,881 + Pf6N2E2_3094 0.32 -0.1 3,582,881 + Pf6N2E2_3094 0.32 +0.9 3,582,882 - Pf6N2E2_3094 0.32 -1.9 3,583,178 + Pf6N2E2_3094 0.70 +0.2 3,583,178 + Pf6N2E2_3094 0.70 -0.8 3,583,178 + Pf6N2E2_3094 0.70 -1.9 3,583,179 - Pf6N2E2_3094 0.70 -1.0 3,583,431 - Pf6N2E2_3095 0.21 -1.2 3,583,553 + +1.2 3,583,553 + -1.6 3,583,554 - -0.2 3,583,554 - +1.0 3,583,665 - Pf6N2E2_3096 0.26 +0.7 3,584,185 + -0.6 3,584,186 - +0.6 3,584,186 - -0.2 3,584,186 - -0.1 3,584,186 - +0.1 3,584,214 - Pf6N2E2_3097 0.13 +0.1 3,584,214 - Pf6N2E2_3097 0.13 +0.8 3,584,214 - Pf6N2E2_3097 0.13 -0.7 3,584,377 + Pf6N2E2_3097 0.36 +0.2 3,584,377 + Pf6N2E2_3097 0.36 +0.8 3,584,377 + Pf6N2E2_3097 0.36 +0.1 3,584,377 + Pf6N2E2_3097 0.36 +1.5 3,584,378 - Pf6N2E2_3097 0.36 -1.2 3,584,378 - Pf6N2E2_3097 0.36 +0.2 3,584,378 - Pf6N2E2_3097 0.36 +0.1 3,584,378 - Pf6N2E2_3097 0.36 -0.1 3,584,378 - Pf6N2E2_3097 0.36 -0.2 3,584,378 - Pf6N2E2_3097 0.36 -0.7 3,584,378 - Pf6N2E2_3097 0.36 +0.6 3,584,378 - Pf6N2E2_3097 0.36 +0.2 3,584,434 + Pf6N2E2_3097 0.44 -0.1 3,584,434 + Pf6N2E2_3097 0.44 -0.3 3,584,434 + Pf6N2E2_3097 0.44 -0.2 3,584,435 - Pf6N2E2_3097 0.44 -0.7 3,584,435 - Pf6N2E2_3097 0.44 +0.2
Or see this region's nucleotide sequence