Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2941 and Pf6N2E2_2942 are separated by 271 nucleotides Pf6N2E2_2942 and Pf6N2E2_2943 are separated by 98 nucleotides Pf6N2E2_2943 and Pf6N2E2_2944 are separated by 63 nucleotides
Pf6N2E2_2941 - Esterase A, at 3,432,878 to 3,434,023
_2941
Pf6N2E2_2942 - ATP-dependent helicase DinG/Rad3, at 3,434,295 to 3,436,439
_2942
Pf6N2E2_2943 - Phosphoribosylcarboxyaminoimidazole (NCAIR) mutase, at 3,436,538 to 3,437,002
_2943
Pf6N2E2_2944 - Collagen pro alpha-chain precursor, at 3,437,066 to 3,437,824
_2944
Position (kb)
3434
3435
3436
3437 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3433.542 kb on + strand, within Pf6N2E2_2941 at 3433.543 kb on - strand, within Pf6N2E2_2941 at 3433.557 kb on + strand, within Pf6N2E2_2941 at 3433.558 kb on - strand, within Pf6N2E2_2941 at 3433.629 kb on + strand, within Pf6N2E2_2941 at 3433.629 kb on + strand, within Pf6N2E2_2941 at 3433.629 kb on + strand, within Pf6N2E2_2941 at 3433.629 kb on + strand, within Pf6N2E2_2941 at 3434.004 kb on + strand at 3434.004 kb on + strand at 3434.573 kb on - strand, within Pf6N2E2_2942 at 3435.010 kb on + strand, within Pf6N2E2_2942 at 3435.176 kb on + strand, within Pf6N2E2_2942 at 3435.176 kb on + strand, within Pf6N2E2_2942 at 3435.177 kb on - strand, within Pf6N2E2_2942 at 3435.215 kb on - strand, within Pf6N2E2_2942 at 3435.610 kb on + strand, within Pf6N2E2_2942 at 3435.610 kb on + strand, within Pf6N2E2_2942 at 3435.611 kb on - strand, within Pf6N2E2_2942 at 3435.611 kb on - strand, within Pf6N2E2_2942 at 3435.611 kb on - strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.668 kb on - strand, within Pf6N2E2_2942 at 3435.668 kb on - strand, within Pf6N2E2_2942 at 3435.668 kb on - strand, within Pf6N2E2_2942 at 3435.668 kb on - strand, within Pf6N2E2_2942 at 3435.668 kb on - strand, within Pf6N2E2_2942 at 3435.668 kb on - strand, within Pf6N2E2_2942 at 3435.784 kb on + strand, within Pf6N2E2_2942 at 3435.784 kb on + strand, within Pf6N2E2_2942 at 3435.784 kb on + strand, within Pf6N2E2_2942 at 3435.784 kb on + strand, within Pf6N2E2_2942 at 3435.784 kb on + strand, within Pf6N2E2_2942 at 3435.785 kb on - strand, within Pf6N2E2_2942 at 3435.785 kb on - strand, within Pf6N2E2_2942 at 3435.785 kb on - strand, within Pf6N2E2_2942 at 3435.833 kb on + strand, within Pf6N2E2_2942 at 3435.908 kb on + strand, within Pf6N2E2_2942 at 3435.909 kb on - strand, within Pf6N2E2_2942 at 3435.909 kb on - strand, within Pf6N2E2_2942 at 3436.060 kb on + strand, within Pf6N2E2_2942 at 3436.060 kb on + strand, within Pf6N2E2_2942 at 3436.060 kb on + strand, within Pf6N2E2_2942 at 3436.061 kb on - strand, within Pf6N2E2_2942 at 3436.061 kb on - strand, within Pf6N2E2_2942 at 3436.061 kb on - strand, within Pf6N2E2_2942 at 3436.136 kb on - strand, within Pf6N2E2_2942 at 3436.136 kb on - strand, within Pf6N2E2_2942 at 3436.136 kb on - strand, within Pf6N2E2_2942 at 3436.225 kb on + strand at 3436.399 kb on + strand at 3436.499 kb on + strand at 3436.499 kb on + strand at 3436.500 kb on - strand at 3436.500 kb on - strand at 3436.559 kb on + strand at 3436.560 kb on - strand at 3436.560 kb on - strand at 3436.560 kb on - strand at 3436.573 kb on - strand at 3436.573 kb on - strand at 3436.678 kb on - strand, within Pf6N2E2_2943 at 3436.743 kb on + strand, within Pf6N2E2_2943 at 3436.744 kb on - strand, within Pf6N2E2_2943 at 3436.790 kb on + strand, within Pf6N2E2_2943 at 3436.790 kb on + strand, within Pf6N2E2_2943 at 3436.790 kb on + strand, within Pf6N2E2_2943 at 3436.814 kb on + strand, within Pf6N2E2_2943 at 3436.814 kb on + strand, within Pf6N2E2_2943 at 3436.814 kb on + strand, within Pf6N2E2_2943 at 3436.814 kb on + strand, within Pf6N2E2_2943 at 3436.814 kb on + strand, within Pf6N2E2_2943 at 3436.814 kb on + strand, within Pf6N2E2_2943 at 3436.814 kb on + strand, within Pf6N2E2_2943 at 3436.814 kb on + strand, within Pf6N2E2_2943 at 3436.815 kb on - strand, within Pf6N2E2_2943 at 3436.815 kb on - strand, within Pf6N2E2_2943 at 3436.815 kb on - strand, within Pf6N2E2_2943 at 3436.815 kb on - strand, within Pf6N2E2_2943 at 3436.815 kb on - strand, within Pf6N2E2_2943 at 3436.815 kb on - strand, within Pf6N2E2_2943 at 3436.815 kb on - strand, within Pf6N2E2_2943 at 3436.815 kb on - strand, within Pf6N2E2_2943 at 3436.815 kb on - strand, within Pf6N2E2_2943 at 3436.873 kb on + strand, within Pf6N2E2_2943 at 3437.011 kb on + strand at 3437.011 kb on + strand at 3437.011 kb on + strand at 3437.011 kb on + strand at 3437.011 kb on + strand at 3437.011 kb on + strand at 3437.012 kb on - strand at 3437.012 kb on - strand at 3437.160 kb on + strand, within Pf6N2E2_2944 at 3437.160 kb on + strand, within Pf6N2E2_2944 at 3437.266 kb on - strand, within Pf6N2E2_2944
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 3,433,542 + Pf6N2E2_2941 0.58 +0.9 3,433,543 - Pf6N2E2_2941 0.58 +0.6 3,433,557 + Pf6N2E2_2941 0.59 +1.1 3,433,558 - Pf6N2E2_2941 0.59 +0.1 3,433,629 + Pf6N2E2_2941 0.66 +0.7 3,433,629 + Pf6N2E2_2941 0.66 -0.6 3,433,629 + Pf6N2E2_2941 0.66 -0.1 3,433,629 + Pf6N2E2_2941 0.66 +0.8 3,434,004 + +0.1 3,434,004 + +0.2 3,434,573 - Pf6N2E2_2942 0.13 +2.2 3,435,010 + Pf6N2E2_2942 0.33 +1.1 3,435,176 + Pf6N2E2_2942 0.41 +1.9 3,435,176 + Pf6N2E2_2942 0.41 +2.2 3,435,177 - Pf6N2E2_2942 0.41 -0.6 3,435,215 - Pf6N2E2_2942 0.43 +2.0 3,435,610 + Pf6N2E2_2942 0.61 -0.3 3,435,610 + Pf6N2E2_2942 0.61 +1.5 3,435,611 - Pf6N2E2_2942 0.61 +1.1 3,435,611 - Pf6N2E2_2942 0.61 +1.6 3,435,611 - Pf6N2E2_2942 0.61 +2.2 3,435,667 + Pf6N2E2_2942 0.64 +2.8 3,435,667 + Pf6N2E2_2942 0.64 +2.2 3,435,667 + Pf6N2E2_2942 0.64 +2.7 3,435,667 + Pf6N2E2_2942 0.64 +0.5 3,435,667 + Pf6N2E2_2942 0.64 +1.7 3,435,667 + Pf6N2E2_2942 0.64 +1.6 3,435,667 + Pf6N2E2_2942 0.64 +1.1 3,435,667 + Pf6N2E2_2942 0.64 +1.7 3,435,667 + Pf6N2E2_2942 0.64 +1.5 3,435,667 + Pf6N2E2_2942 0.64 +2.1 3,435,668 - Pf6N2E2_2942 0.64 +1.2 3,435,668 - Pf6N2E2_2942 0.64 +0.9 3,435,668 - Pf6N2E2_2942 0.64 +2.7 3,435,668 - Pf6N2E2_2942 0.64 +1.6 3,435,668 - Pf6N2E2_2942 0.64 +2.4 3,435,668 - Pf6N2E2_2942 0.64 +2.5 3,435,784 + Pf6N2E2_2942 0.69 +1.5 3,435,784 + Pf6N2E2_2942 0.69 +1.5 3,435,784 + Pf6N2E2_2942 0.69 +1.7 3,435,784 + Pf6N2E2_2942 0.69 +1.1 3,435,784 + Pf6N2E2_2942 0.69 +1.9 3,435,785 - Pf6N2E2_2942 0.69 +2.5 3,435,785 - Pf6N2E2_2942 0.69 +1.8 3,435,785 - Pf6N2E2_2942 0.69 +1.8 3,435,833 + Pf6N2E2_2942 0.72 +2.5 3,435,908 + Pf6N2E2_2942 0.75 +2.1 3,435,909 - Pf6N2E2_2942 0.75 +1.2 3,435,909 - Pf6N2E2_2942 0.75 +1.7 3,436,060 + Pf6N2E2_2942 0.82 +2.2 3,436,060 + Pf6N2E2_2942 0.82 +1.6 3,436,060 + Pf6N2E2_2942 0.82 +2.6 3,436,061 - Pf6N2E2_2942 0.82 +1.8 3,436,061 - Pf6N2E2_2942 0.82 +0.7 3,436,061 - Pf6N2E2_2942 0.82 +2.3 3,436,136 - Pf6N2E2_2942 0.86 +2.6 3,436,136 - Pf6N2E2_2942 0.86 +1.7 3,436,136 - Pf6N2E2_2942 0.86 +2.2 3,436,225 + +1.6 3,436,399 + +4.6 3,436,499 + +1.6 3,436,499 + +0.9 3,436,500 - -3.0 3,436,500 - -2.2 3,436,559 + +0.3 3,436,560 - -0.2 3,436,560 - -2.8 3,436,560 - -1.0 3,436,573 - -0.4 3,436,573 - -2.5 3,436,678 - Pf6N2E2_2943 0.30 -1.6 3,436,743 + Pf6N2E2_2943 0.44 +0.7 3,436,744 - Pf6N2E2_2943 0.44 -0.6 3,436,790 + Pf6N2E2_2943 0.54 +0.5 3,436,790 + Pf6N2E2_2943 0.54 +0.4 3,436,790 + Pf6N2E2_2943 0.54 -0.2 3,436,814 + Pf6N2E2_2943 0.59 -0.2 3,436,814 + Pf6N2E2_2943 0.59 +1.0 3,436,814 + Pf6N2E2_2943 0.59 +0.3 3,436,814 + Pf6N2E2_2943 0.59 +1.0 3,436,814 + Pf6N2E2_2943 0.59 -0.6 3,436,814 + Pf6N2E2_2943 0.59 -0.1 3,436,814 + Pf6N2E2_2943 0.59 +0.3 3,436,814 + Pf6N2E2_2943 0.59 +0.8 3,436,815 - Pf6N2E2_2943 0.60 +0.4 3,436,815 - Pf6N2E2_2943 0.60 -1.2 3,436,815 - Pf6N2E2_2943 0.60 -1.7 3,436,815 - Pf6N2E2_2943 0.60 +0.1 3,436,815 - Pf6N2E2_2943 0.60 +0.7 3,436,815 - Pf6N2E2_2943 0.60 -0.4 3,436,815 - Pf6N2E2_2943 0.60 -1.4 3,436,815 - Pf6N2E2_2943 0.60 -2.4 3,436,815 - Pf6N2E2_2943 0.60 -3.3 3,436,873 + Pf6N2E2_2943 0.72 -1.1 3,437,011 + -0.7 3,437,011 + -0.6 3,437,011 + -0.5 3,437,011 + -0.1 3,437,011 + +0.7 3,437,011 + -0.1 3,437,012 - +0.4 3,437,012 - +0.1 3,437,160 + Pf6N2E2_2944 0.12 -0.1 3,437,160 + Pf6N2E2_2944 0.12 -0.9 3,437,266 - Pf6N2E2_2944 0.26 -0.4
Or see this region's nucleotide sequence