Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2754 and Pf6N2E2_2755 are separated by 12 nucleotides Pf6N2E2_2755 and Pf6N2E2_2756 are separated by 31 nucleotides
Pf6N2E2_2754 - Sigma factor RpoE negative regulatory protein RseB precursor, at 3,246,655 to 3,247,602
_2754
Pf6N2E2_2755 - Sigma factor RpoE negative regulatory protein RseA, at 3,247,615 to 3,248,202
_2755
Pf6N2E2_2756 - RNA polymerase sigma-H factor AlgT, at 3,248,234 to 3,248,815
_2756
Position (kb)
3247
3248
3249 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3246.873 kb on - strand, within Pf6N2E2_2754 at 3246.873 kb on - strand, within Pf6N2E2_2754 at 3247.303 kb on - strand, within Pf6N2E2_2754 at 3247.344 kb on + strand, within Pf6N2E2_2754 at 3247.344 kb on + strand, within Pf6N2E2_2754 at 3247.438 kb on - strand, within Pf6N2E2_2754 at 3247.592 kb on - strand at 3247.592 kb on - strand at 3247.703 kb on + strand, within Pf6N2E2_2755 at 3247.704 kb on - strand, within Pf6N2E2_2755 at 3248.236 kb on - strand at 3248.236 kb on - strand at 3248.312 kb on + strand, within Pf6N2E2_2756 at 3248.313 kb on - strand, within Pf6N2E2_2756 at 3248.347 kb on - strand, within Pf6N2E2_2756 at 3248.369 kb on + strand, within Pf6N2E2_2756 at 3248.376 kb on - strand, within Pf6N2E2_2756 at 3248.497 kb on - strand, within Pf6N2E2_2756 at 3248.497 kb on - strand, within Pf6N2E2_2756 at 3248.497 kb on - strand, within Pf6N2E2_2756 at 3248.497 kb on - strand, within Pf6N2E2_2756 at 3248.497 kb on - strand, within Pf6N2E2_2756 at 3248.497 kb on - strand, within Pf6N2E2_2756 at 3248.497 kb on - strand, within Pf6N2E2_2756 at 3248.497 kb on - strand, within Pf6N2E2_2756 at 3248.497 kb on - strand, within Pf6N2E2_2756 at 3248.497 kb on - strand, within Pf6N2E2_2756 at 3248.520 kb on - strand, within Pf6N2E2_2756 at 3248.520 kb on - strand, within Pf6N2E2_2756 at 3248.520 kb on - strand, within Pf6N2E2_2756 at 3248.520 kb on - strand, within Pf6N2E2_2756 at 3248.602 kb on - strand, within Pf6N2E2_2756 at 3248.731 kb on - strand, within Pf6N2E2_2756 at 3248.731 kb on - strand, within Pf6N2E2_2756 at 3248.731 kb on - strand, within Pf6N2E2_2756 at 3248.734 kb on + strand, within Pf6N2E2_2756 at 3248.735 kb on - strand, within Pf6N2E2_2756 at 3248.852 kb on - strand at 3248.854 kb on + strand at 3248.855 kb on - strand at 3248.855 kb on - strand at 3248.876 kb on + strand at 3248.877 kb on - strand at 3248.985 kb on - strand at 3249.069 kb on - strand at 3249.069 kb on - strand at 3249.078 kb on - strand at 3249.078 kb on - strand at 3249.105 kb on + strand at 3249.105 kb on + strand at 3249.105 kb on + strand at 3249.105 kb on + strand at 3249.106 kb on - strand at 3249.106 kb on - strand at 3249.106 kb on - strand at 3249.107 kb on + strand at 3249.115 kb on + strand at 3249.116 kb on - strand at 3249.116 kb on - strand at 3249.116 kb on - strand at 3249.124 kb on + strand at 3249.125 kb on - strand at 3249.125 kb on - strand at 3249.145 kb on + strand at 3249.145 kb on + strand at 3249.146 kb on - strand at 3249.146 kb on - strand at 3249.146 kb on - strand at 3249.146 kb on - strand at 3249.146 kb on - strand at 3249.146 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 3,246,873 - Pf6N2E2_2754 0.23 -0.1 3,246,873 - Pf6N2E2_2754 0.23 -0.6 3,247,303 - Pf6N2E2_2754 0.68 -0.5 3,247,344 + Pf6N2E2_2754 0.73 -0.2 3,247,344 + Pf6N2E2_2754 0.73 -0.7 3,247,438 - Pf6N2E2_2754 0.83 +0.8 3,247,592 - -0.2 3,247,592 - -0.9 3,247,703 + Pf6N2E2_2755 0.15 -0.6 3,247,704 - Pf6N2E2_2755 0.15 -1.8 3,248,236 - +0.1 3,248,236 - -1.5 3,248,312 + Pf6N2E2_2756 0.13 -1.3 3,248,313 - Pf6N2E2_2756 0.14 -1.3 3,248,347 - Pf6N2E2_2756 0.19 -0.6 3,248,369 + Pf6N2E2_2756 0.23 -0.1 3,248,376 - Pf6N2E2_2756 0.24 -0.2 3,248,497 - Pf6N2E2_2756 0.45 -0.2 3,248,497 - Pf6N2E2_2756 0.45 +0.0 3,248,497 - Pf6N2E2_2756 0.45 -1.7 3,248,497 - Pf6N2E2_2756 0.45 -1.4 3,248,497 - Pf6N2E2_2756 0.45 -1.7 3,248,497 - Pf6N2E2_2756 0.45 -0.4 3,248,497 - Pf6N2E2_2756 0.45 -0.1 3,248,497 - Pf6N2E2_2756 0.45 +0.7 3,248,497 - Pf6N2E2_2756 0.45 -1.0 3,248,497 - Pf6N2E2_2756 0.45 -1.3 3,248,520 - Pf6N2E2_2756 0.49 -0.6 3,248,520 - Pf6N2E2_2756 0.49 -0.8 3,248,520 - Pf6N2E2_2756 0.49 +0.3 3,248,520 - Pf6N2E2_2756 0.49 +0.2 3,248,602 - Pf6N2E2_2756 0.63 -2.3 3,248,731 - Pf6N2E2_2756 0.85 -1.7 3,248,731 - Pf6N2E2_2756 0.85 -1.6 3,248,731 - Pf6N2E2_2756 0.85 -2.2 3,248,734 + Pf6N2E2_2756 0.86 -3.0 3,248,735 - Pf6N2E2_2756 0.86 +0.2 3,248,852 - +0.8 3,248,854 + +0.9 3,248,855 - +0.4 3,248,855 - -3.1 3,248,876 + +0.8 3,248,877 - -1.7 3,248,985 - +1.6 3,249,069 - +0.4 3,249,069 - +2.0 3,249,078 - +1.0 3,249,078 - -0.4 3,249,105 + +0.2 3,249,105 + -0.8 3,249,105 + +1.0 3,249,105 + -0.0 3,249,106 - +2.7 3,249,106 - -0.3 3,249,106 - +1.4 3,249,107 + -1.6 3,249,115 + +0.7 3,249,116 - +1.2 3,249,116 - +1.5 3,249,116 - +1.0 3,249,124 + -0.0 3,249,125 - +0.6 3,249,125 - +1.0 3,249,145 + +0.1 3,249,145 + -0.7 3,249,146 - +0.8 3,249,146 - -1.3 3,249,146 - +0.8 3,249,146 - -0.0 3,249,146 - -1.1 3,249,146 - +1.4
Or see this region's nucleotide sequence