Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2040 and Pf6N2E2_2041 are separated by 7 nucleotides Pf6N2E2_2041 and Pf6N2E2_2042 are separated by 190 nucleotides
Pf6N2E2_2040 - D-beta-hydroxybutyrate permease, at 2,429,187 to 2,430,578
_2040
Pf6N2E2_2041 - D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30), at 2,430,586 to 2,431,362
_2041
Pf6N2E2_2042 - Acetoacetyl-CoA synthetase (EC 6.2.1.16), at 2,431,553 to 2,433,508
_2042
Position (kb)
2430
2431
2432 Strain fitness (log2 ratio)
-1
0
1
2 at 2429.588 kb on + strand, within Pf6N2E2_2040 at 2429.588 kb on + strand, within Pf6N2E2_2040 at 2429.589 kb on - strand, within Pf6N2E2_2040 at 2429.589 kb on - strand, within Pf6N2E2_2040 at 2429.591 kb on + strand, within Pf6N2E2_2040 at 2429.591 kb on + strand, within Pf6N2E2_2040 at 2429.591 kb on + strand, within Pf6N2E2_2040 at 2429.591 kb on + strand, within Pf6N2E2_2040 at 2429.592 kb on - strand, within Pf6N2E2_2040 at 2429.592 kb on - strand, within Pf6N2E2_2040 at 2430.076 kb on + strand, within Pf6N2E2_2040 at 2430.214 kb on + strand, within Pf6N2E2_2040 at 2430.214 kb on + strand, within Pf6N2E2_2040 at 2430.452 kb on + strand at 2430.452 kb on + strand at 2430.453 kb on - strand at 2430.453 kb on - strand at 2430.512 kb on + strand at 2430.512 kb on + strand at 2430.513 kb on - strand at 2430.513 kb on - strand at 2430.559 kb on - strand at 2431.045 kb on + strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.292 kb on + strand at 2431.293 kb on - strand at 2431.293 kb on - strand at 2431.293 kb on - strand at 2431.335 kb on + strand at 2431.336 kb on - strand at 2431.361 kb on - strand at 2431.498 kb on + strand at 2431.499 kb on - strand at 2431.502 kb on + strand at 2431.503 kb on - strand at 2431.503 kb on - strand at 2431.503 kb on - strand at 2431.503 kb on - strand at 2431.524 kb on + strand at 2431.524 kb on + strand at 2431.524 kb on + strand at 2431.525 kb on - strand at 2431.525 kb on - strand at 2431.525 kb on - strand at 2432.231 kb on + strand, within Pf6N2E2_2042 at 2432.232 kb on - strand, within Pf6N2E2_2042 at 2432.354 kb on + strand, within Pf6N2E2_2042 at 2432.354 kb on + strand, within Pf6N2E2_2042 at 2432.354 kb on + strand, within Pf6N2E2_2042 at 2432.354 kb on + strand, within Pf6N2E2_2042 at 2432.355 kb on - strand, within Pf6N2E2_2042 at 2432.355 kb on - strand, within Pf6N2E2_2042 at 2432.355 kb on - strand, within Pf6N2E2_2042
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 2,429,588 + Pf6N2E2_2040 0.29 -0.2 2,429,588 + Pf6N2E2_2040 0.29 +0.5 2,429,589 - Pf6N2E2_2040 0.29 +0.1 2,429,589 - Pf6N2E2_2040 0.29 +0.7 2,429,591 + Pf6N2E2_2040 0.29 -1.5 2,429,591 + Pf6N2E2_2040 0.29 -0.0 2,429,591 + Pf6N2E2_2040 0.29 +1.1 2,429,591 + Pf6N2E2_2040 0.29 -0.9 2,429,592 - Pf6N2E2_2040 0.29 +0.1 2,429,592 - Pf6N2E2_2040 0.29 +1.0 2,430,076 + Pf6N2E2_2040 0.64 +0.3 2,430,214 + Pf6N2E2_2040 0.74 +0.5 2,430,214 + Pf6N2E2_2040 0.74 -1.0 2,430,452 + +0.9 2,430,452 + -0.1 2,430,453 - -0.5 2,430,453 - +0.1 2,430,512 + -0.6 2,430,512 + -0.4 2,430,513 - +0.5 2,430,513 - +0.1 2,430,559 - -0.5 2,431,045 + Pf6N2E2_2041 0.59 +0.7 2,431,046 - Pf6N2E2_2041 0.59 -1.1 2,431,046 - Pf6N2E2_2041 0.59 -1.7 2,431,046 - Pf6N2E2_2041 0.59 -0.6 2,431,046 - Pf6N2E2_2041 0.59 -1.3 2,431,046 - Pf6N2E2_2041 0.59 +0.1 2,431,292 + -0.9 2,431,293 - -0.8 2,431,293 - -1.6 2,431,293 - +1.2 2,431,335 + -0.0 2,431,336 - +0.1 2,431,361 - +0.4 2,431,498 + -0.4 2,431,499 - +0.3 2,431,502 + +0.1 2,431,503 - +0.2 2,431,503 - -0.8 2,431,503 - +0.9 2,431,503 - -1.4 2,431,524 + -1.0 2,431,524 + +0.0 2,431,524 + +1.9 2,431,525 - -0.0 2,431,525 - -1.7 2,431,525 - -0.0 2,432,231 + Pf6N2E2_2042 0.35 -1.1 2,432,232 - Pf6N2E2_2042 0.35 +0.5 2,432,354 + Pf6N2E2_2042 0.41 -0.8 2,432,354 + Pf6N2E2_2042 0.41 +0.3 2,432,354 + Pf6N2E2_2042 0.41 -0.6 2,432,354 + Pf6N2E2_2042 0.41 +0.2 2,432,355 - Pf6N2E2_2042 0.41 +0.0 2,432,355 - Pf6N2E2_2042 0.41 -1.5 2,432,355 - Pf6N2E2_2042 0.41 -0.1
Or see this region's nucleotide sequence