Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1965 and Pf6N2E2_1966 are separated by 841 nucleotides Pf6N2E2_1966 and Pf6N2E2_1967 are separated by 452 nucleotides
Pf6N2E2_1965 - Fructokinase (EC 2.7.1.4) (from data) , at 2,330,391 to 2,331,311
_1965
Pf6N2E2_1966 - SAM-dependent methyltransferases, at 2,332,153 to 2,332,833
_1966
Pf6N2E2_1967 - Cobalt-zinc-cadmium resistance protein, at 2,333,286 to 2,333,915
_1967
Position (kb)
2332
2333 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2331.173 kb on - strand, within Pf6N2E2_1965 at 2331.564 kb on + strand at 2331.570 kb on + strand at 2331.572 kb on + strand at 2331.642 kb on + strand at 2331.643 kb on - strand at 2331.643 kb on - strand at 2331.707 kb on + strand at 2331.726 kb on - strand at 2331.726 kb on - strand at 2331.726 kb on - strand at 2331.786 kb on + strand at 2331.787 kb on - strand at 2331.835 kb on + strand at 2331.836 kb on - strand at 2331.899 kb on - strand at 2331.911 kb on + strand at 2331.911 kb on + strand at 2331.914 kb on - strand at 2331.929 kb on + strand at 2331.929 kb on + strand at 2331.930 kb on - strand at 2332.102 kb on + strand at 2332.103 kb on - strand at 2332.103 kb on - strand at 2332.213 kb on + strand at 2332.217 kb on + strand at 2332.217 kb on + strand at 2332.218 kb on - strand at 2332.593 kb on + strand, within Pf6N2E2_1966 at 2332.611 kb on + strand, within Pf6N2E2_1966 at 2332.611 kb on + strand, within Pf6N2E2_1966 at 2332.612 kb on - strand, within Pf6N2E2_1966 at 2332.716 kb on - strand, within Pf6N2E2_1966 at 2332.785 kb on + strand at 2332.795 kb on + strand at 2332.817 kb on + strand at 2332.854 kb on + strand at 2332.854 kb on + strand at 2332.927 kb on + strand at 2332.928 kb on - strand at 2332.951 kb on + strand at 2332.951 kb on + strand at 2333.124 kb on - strand at 2333.124 kb on - strand at 2333.159 kb on - strand at 2333.193 kb on - strand at 2333.213 kb on - strand at 2333.213 kb on - strand at 2333.244 kb on + strand at 2333.245 kb on - strand at 2333.254 kb on + strand at 2333.254 kb on + strand at 2333.254 kb on + strand at 2333.254 kb on + strand at 2333.255 kb on - strand at 2333.255 kb on - strand at 2333.255 kb on - strand at 2333.255 kb on - strand at 2333.470 kb on + strand, within Pf6N2E2_1967 at 2333.470 kb on + strand, within Pf6N2E2_1967 at 2333.470 kb on + strand, within Pf6N2E2_1967 at 2333.471 kb on - strand, within Pf6N2E2_1967 at 2333.471 kb on - strand, within Pf6N2E2_1967 at 2333.479 kb on + strand, within Pf6N2E2_1967 at 2333.479 kb on + strand, within Pf6N2E2_1967 at 2333.479 kb on + strand, within Pf6N2E2_1967 at 2333.479 kb on + strand, within Pf6N2E2_1967 at 2333.479 kb on + strand, within Pf6N2E2_1967 at 2333.480 kb on - strand, within Pf6N2E2_1967 at 2333.499 kb on + strand, within Pf6N2E2_1967 at 2333.500 kb on - strand, within Pf6N2E2_1967 at 2333.562 kb on + strand, within Pf6N2E2_1967 at 2333.563 kb on - strand, within Pf6N2E2_1967 at 2333.647 kb on + strand, within Pf6N2E2_1967 at 2333.647 kb on + strand, within Pf6N2E2_1967 at 2333.647 kb on + strand, within Pf6N2E2_1967 at 2333.647 kb on + strand, within Pf6N2E2_1967 at 2333.647 kb on + strand, within Pf6N2E2_1967 at 2333.647 kb on + strand, within Pf6N2E2_1967 at 2333.648 kb on - strand, within Pf6N2E2_1967 at 2333.648 kb on - strand, within Pf6N2E2_1967 at 2333.648 kb on - strand, within Pf6N2E2_1967 at 2333.648 kb on - strand, within Pf6N2E2_1967 at 2333.648 kb on - strand, within Pf6N2E2_1967 at 2333.648 kb on - strand, within Pf6N2E2_1967 at 2333.801 kb on - strand, within Pf6N2E2_1967 at 2333.809 kb on + strand, within Pf6N2E2_1967
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 2,331,173 - Pf6N2E2_1965 0.85 +0.4 2,331,564 + -0.3 2,331,570 + +0.7 2,331,572 + +0.5 2,331,642 + -0.4 2,331,643 - -1.2 2,331,643 - +0.1 2,331,707 + +2.3 2,331,726 - -1.5 2,331,726 - -0.6 2,331,726 - +0.9 2,331,786 + +2.2 2,331,787 - -0.4 2,331,835 + -0.5 2,331,836 - -3.6 2,331,899 - +0.8 2,331,911 + +0.7 2,331,911 + +0.3 2,331,914 - -0.2 2,331,929 + +1.3 2,331,929 + +0.4 2,331,930 - +0.3 2,332,102 + -0.3 2,332,103 - +0.4 2,332,103 - +1.0 2,332,213 + -1.4 2,332,217 + -1.6 2,332,217 + -0.0 2,332,218 - +2.4 2,332,593 + Pf6N2E2_1966 0.65 -0.8 2,332,611 + Pf6N2E2_1966 0.67 -0.3 2,332,611 + Pf6N2E2_1966 0.67 -0.4 2,332,612 - Pf6N2E2_1966 0.67 -1.4 2,332,716 - Pf6N2E2_1966 0.83 -1.6 2,332,785 + -0.8 2,332,795 + -2.1 2,332,817 + +1.3 2,332,854 + +0.7 2,332,854 + +0.7 2,332,927 + -0.8 2,332,928 - -0.0 2,332,951 + -1.2 2,332,951 + -1.2 2,333,124 - -1.9 2,333,124 - -0.1 2,333,159 - +0.8 2,333,193 - +1.5 2,333,213 - +0.6 2,333,213 - +0.8 2,333,244 + -0.8 2,333,245 - +0.2 2,333,254 + -0.4 2,333,254 + +0.8 2,333,254 + -0.7 2,333,254 + -0.4 2,333,255 - +0.8 2,333,255 - -0.6 2,333,255 - -2.1 2,333,255 - +0.4 2,333,470 + Pf6N2E2_1967 0.29 +2.1 2,333,470 + Pf6N2E2_1967 0.29 -0.0 2,333,470 + Pf6N2E2_1967 0.29 +1.3 2,333,471 - Pf6N2E2_1967 0.29 +0.2 2,333,471 - Pf6N2E2_1967 0.29 +0.1 2,333,479 + Pf6N2E2_1967 0.31 -0.8 2,333,479 + Pf6N2E2_1967 0.31 -0.4 2,333,479 + Pf6N2E2_1967 0.31 -1.8 2,333,479 + Pf6N2E2_1967 0.31 -0.1 2,333,479 + Pf6N2E2_1967 0.31 -0.0 2,333,480 - Pf6N2E2_1967 0.31 -2.2 2,333,499 + Pf6N2E2_1967 0.34 +0.5 2,333,500 - Pf6N2E2_1967 0.34 +0.8 2,333,562 + Pf6N2E2_1967 0.44 +0.2 2,333,563 - Pf6N2E2_1967 0.44 -0.5 2,333,647 + Pf6N2E2_1967 0.57 +0.5 2,333,647 + Pf6N2E2_1967 0.57 -1.0 2,333,647 + Pf6N2E2_1967 0.57 +0.7 2,333,647 + Pf6N2E2_1967 0.57 +0.7 2,333,647 + Pf6N2E2_1967 0.57 +0.1 2,333,647 + Pf6N2E2_1967 0.57 +0.0 2,333,648 - Pf6N2E2_1967 0.57 -0.5 2,333,648 - Pf6N2E2_1967 0.57 -0.8 2,333,648 - Pf6N2E2_1967 0.57 +0.7 2,333,648 - Pf6N2E2_1967 0.57 -0.9 2,333,648 - Pf6N2E2_1967 0.57 +0.9 2,333,648 - Pf6N2E2_1967 0.57 +1.9 2,333,801 - Pf6N2E2_1967 0.82 -1.0 2,333,809 + Pf6N2E2_1967 0.83 -0.9
Or see this region's nucleotide sequence