Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1755 and Pf6N2E2_1756 are separated by 73 nucleotides Pf6N2E2_1756 and Pf6N2E2_1757 are separated by 106 nucleotides Pf6N2E2_1757 and Pf6N2E2_1758 are separated by 83 nucleotides Pf6N2E2_1758 and Pf6N2E2_1759 are separated by 8 nucleotides
Pf6N2E2_1755 - 2-Oxobutyrate oxidase, putative, at 2,073,481 to 2,074,533
_1755
Pf6N2E2_1756 - Arginine ABC transporter, periplasmic arginine-binding protein ArtJ, at 2,074,607 to 2,075,413
_1756
Pf6N2E2_1757 - glutamine ABC transporter ATP-binding component, at 2,075,520 to 2,077,028
_1757
Pf6N2E2_1758 - Transcriptional regulator, MarR family, at 2,077,112 to 2,077,567
_1758
Pf6N2E2_1759 - uncharacterized domain 1, at 2,077,576 to 2,078,187
_1759
Position (kb)
2075
2076
2077
2078 Strain fitness (log2 ratio)
-2
-1
0
1 at 2074.628 kb on - strand at 2074.628 kb on - strand at 2074.755 kb on - strand, within Pf6N2E2_1756 at 2074.887 kb on - strand, within Pf6N2E2_1756 at 2074.887 kb on - strand, within Pf6N2E2_1756 at 2074.910 kb on + strand, within Pf6N2E2_1756 at 2074.911 kb on - strand, within Pf6N2E2_1756 at 2074.911 kb on - strand, within Pf6N2E2_1756 at 2074.911 kb on - strand, within Pf6N2E2_1756 at 2074.911 kb on - strand, within Pf6N2E2_1756 at 2074.953 kb on - strand, within Pf6N2E2_1756 at 2075.093 kb on + strand, within Pf6N2E2_1756 at 2075.230 kb on + strand, within Pf6N2E2_1756 at 2075.231 kb on - strand, within Pf6N2E2_1756 at 2075.231 kb on - strand, within Pf6N2E2_1756 at 2075.270 kb on + strand, within Pf6N2E2_1756 at 2075.270 kb on + strand, within Pf6N2E2_1756 at 2075.270 kb on + strand, within Pf6N2E2_1756 at 2075.271 kb on - strand, within Pf6N2E2_1756 at 2075.288 kb on - strand, within Pf6N2E2_1756 at 2075.292 kb on - strand, within Pf6N2E2_1756 at 2075.318 kb on + strand, within Pf6N2E2_1756 at 2075.388 kb on - strand at 2075.481 kb on - strand at 2075.481 kb on - strand at 2075.481 kb on - strand at 2075.515 kb on + strand at 2075.586 kb on - strand at 2075.699 kb on + strand, within Pf6N2E2_1757 at 2075.700 kb on - strand, within Pf6N2E2_1757 at 2075.748 kb on + strand, within Pf6N2E2_1757 at 2075.786 kb on - strand, within Pf6N2E2_1757 at 2075.841 kb on + strand, within Pf6N2E2_1757 at 2075.994 kb on + strand, within Pf6N2E2_1757 at 2075.994 kb on + strand, within Pf6N2E2_1757 at 2076.120 kb on + strand, within Pf6N2E2_1757 at 2076.120 kb on + strand, within Pf6N2E2_1757 at 2076.121 kb on - strand, within Pf6N2E2_1757 at 2076.123 kb on + strand, within Pf6N2E2_1757 at 2076.123 kb on + strand, within Pf6N2E2_1757 at 2076.123 kb on + strand, within Pf6N2E2_1757 at 2076.123 kb on + strand, within Pf6N2E2_1757 at 2076.124 kb on - strand, within Pf6N2E2_1757 at 2076.376 kb on + strand, within Pf6N2E2_1757 at 2076.377 kb on - strand, within Pf6N2E2_1757 at 2076.377 kb on - strand, within Pf6N2E2_1757 at 2076.377 kb on - strand, within Pf6N2E2_1757 at 2076.492 kb on + strand, within Pf6N2E2_1757 at 2076.493 kb on - strand, within Pf6N2E2_1757 at 2076.493 kb on - strand, within Pf6N2E2_1757 at 2076.495 kb on - strand, within Pf6N2E2_1757 at 2076.801 kb on - strand, within Pf6N2E2_1757 at 2077.037 kb on + strand at 2077.038 kb on - strand at 2077.376 kb on - strand, within Pf6N2E2_1758 at 2077.450 kb on - strand, within Pf6N2E2_1758 at 2077.558 kb on + strand at 2077.558 kb on + strand at 2077.572 kb on + strand at 2077.867 kb on + strand, within Pf6N2E2_1759 at 2077.868 kb on - strand, within Pf6N2E2_1759
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 2,074,628 - -0.6 2,074,628 - -0.4 2,074,755 - Pf6N2E2_1756 0.18 -1.3 2,074,887 - Pf6N2E2_1756 0.35 +0.1 2,074,887 - Pf6N2E2_1756 0.35 +0.1 2,074,910 + Pf6N2E2_1756 0.38 -0.7 2,074,911 - Pf6N2E2_1756 0.38 +0.3 2,074,911 - Pf6N2E2_1756 0.38 -0.9 2,074,911 - Pf6N2E2_1756 0.38 +0.6 2,074,911 - Pf6N2E2_1756 0.38 -1.1 2,074,953 - Pf6N2E2_1756 0.43 -1.2 2,075,093 + Pf6N2E2_1756 0.60 -0.3 2,075,230 + Pf6N2E2_1756 0.77 -0.4 2,075,231 - Pf6N2E2_1756 0.77 -0.4 2,075,231 - Pf6N2E2_1756 0.77 +1.2 2,075,270 + Pf6N2E2_1756 0.82 -1.0 2,075,270 + Pf6N2E2_1756 0.82 +0.2 2,075,270 + Pf6N2E2_1756 0.82 -0.4 2,075,271 - Pf6N2E2_1756 0.82 +0.2 2,075,288 - Pf6N2E2_1756 0.84 -0.9 2,075,292 - Pf6N2E2_1756 0.85 -0.1 2,075,318 + Pf6N2E2_1756 0.88 +0.0 2,075,388 - +0.7 2,075,481 - +0.1 2,075,481 - -0.7 2,075,481 - -0.1 2,075,515 + +0.3 2,075,586 - -0.5 2,075,699 + Pf6N2E2_1757 0.12 -0.5 2,075,700 - Pf6N2E2_1757 0.12 +0.4 2,075,748 + Pf6N2E2_1757 0.15 +0.8 2,075,786 - Pf6N2E2_1757 0.18 +0.2 2,075,841 + Pf6N2E2_1757 0.21 -0.6 2,075,994 + Pf6N2E2_1757 0.31 +0.4 2,075,994 + Pf6N2E2_1757 0.31 -1.2 2,076,120 + Pf6N2E2_1757 0.40 -1.7 2,076,120 + Pf6N2E2_1757 0.40 +1.2 2,076,121 - Pf6N2E2_1757 0.40 +0.9 2,076,123 + Pf6N2E2_1757 0.40 +0.6 2,076,123 + Pf6N2E2_1757 0.40 -0.1 2,076,123 + Pf6N2E2_1757 0.40 -1.7 2,076,123 + Pf6N2E2_1757 0.40 -0.3 2,076,124 - Pf6N2E2_1757 0.40 +0.1 2,076,376 + Pf6N2E2_1757 0.57 +0.1 2,076,377 - Pf6N2E2_1757 0.57 -0.7 2,076,377 - Pf6N2E2_1757 0.57 -0.6 2,076,377 - Pf6N2E2_1757 0.57 -1.9 2,076,492 + Pf6N2E2_1757 0.64 +0.2 2,076,493 - Pf6N2E2_1757 0.64 -1.1 2,076,493 - Pf6N2E2_1757 0.64 -0.3 2,076,495 - Pf6N2E2_1757 0.65 -0.1 2,076,801 - Pf6N2E2_1757 0.85 -1.7 2,077,037 + -0.9 2,077,038 - +0.1 2,077,376 - Pf6N2E2_1758 0.58 +1.2 2,077,450 - Pf6N2E2_1758 0.74 -0.9 2,077,558 + -0.5 2,077,558 + +1.1 2,077,572 + -0.9 2,077,867 + Pf6N2E2_1759 0.48 -2.6 2,077,868 - Pf6N2E2_1759 0.48 +0.5
Or see this region's nucleotide sequence