Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_168

Experiment: Nalidixic 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_167 and Pf6N2E2_168 are separated by 48 nucleotidesPf6N2E2_168 and Pf6N2E2_169 are separated by 128 nucleotidesPf6N2E2_169 and Pf6N2E2_170 are separated by 162 nucleotidesPf6N2E2_170 and Pf6N2E2_171 are separated by 68 nucleotides Pf6N2E2_167 - Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1), at 220,216 to 221,244 _167 Pf6N2E2_168 - hypothetical protein, at 221,293 to 221,490 _168 Pf6N2E2_169 - FIG00955358: hypothetical protein, at 221,619 to 221,924 _169 Pf6N2E2_170 - lipoprotein, putative, at 222,087 to 222,401 _170 Pf6N2E2_171 - Cold shock protein CspG, at 222,470 to 222,682 _171 Position (kb) 221 222Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 220.393 kb on - strand, within Pf6N2E2_167at 220.393 kb on - strand, within Pf6N2E2_167at 220.393 kb on - strand, within Pf6N2E2_167at 220.393 kb on - strand, within Pf6N2E2_167at 220.495 kb on + strand, within Pf6N2E2_167at 220.496 kb on - strand, within Pf6N2E2_167at 220.580 kb on + strand, within Pf6N2E2_167at 220.581 kb on - strand, within Pf6N2E2_167at 220.581 kb on - strand, within Pf6N2E2_167at 220.624 kb on + strand, within Pf6N2E2_167at 220.785 kb on + strand, within Pf6N2E2_167at 220.785 kb on + strand, within Pf6N2E2_167at 220.867 kb on + strand, within Pf6N2E2_167at 220.978 kb on + strand, within Pf6N2E2_167at 220.978 kb on + strand, within Pf6N2E2_167at 220.979 kb on - strand, within Pf6N2E2_167at 220.979 kb on - strand, within Pf6N2E2_167at 220.979 kb on - strand, within Pf6N2E2_167at 220.979 kb on - strand, within Pf6N2E2_167at 220.979 kb on - strand, within Pf6N2E2_167at 220.979 kb on - strand, within Pf6N2E2_167at 221.002 kb on + strand, within Pf6N2E2_167at 221.002 kb on + strand, within Pf6N2E2_167at 221.144 kb on - strandat 221.144 kb on - strandat 221.255 kb on + strandat 221.255 kb on + strandat 221.256 kb on - strandat 221.256 kb on - strandat 221.257 kb on + strandat 221.258 kb on - strandat 221.258 kb on - strandat 221.389 kb on + strand, within Pf6N2E2_168at 221.389 kb on + strand, within Pf6N2E2_168at 221.429 kb on + strand, within Pf6N2E2_168at 221.429 kb on + strand, within Pf6N2E2_168at 221.429 kb on + strand, within Pf6N2E2_168at 221.430 kb on - strand, within Pf6N2E2_168at 221.430 kb on - strand, within Pf6N2E2_168at 221.430 kb on - strand, within Pf6N2E2_168at 221.436 kb on + strand, within Pf6N2E2_168at 221.436 kb on + strand, within Pf6N2E2_168at 221.437 kb on - strand, within Pf6N2E2_168at 221.493 kb on + strandat 221.493 kb on + strandat 221.494 kb on - strandat 221.517 kb on + strandat 221.517 kb on + strandat 221.517 kb on + strandat 221.518 kb on - strandat 221.518 kb on - strandat 221.518 kb on - strandat 221.518 kb on - strandat 221.527 kb on + strandat 221.528 kb on - strandat 221.561 kb on + strandat 221.562 kb on - strandat 221.562 kb on - strandat 221.702 kb on + strand, within Pf6N2E2_169at 221.703 kb on - strand, within Pf6N2E2_169at 221.736 kb on + strand, within Pf6N2E2_169at 221.737 kb on - strand, within Pf6N2E2_169at 221.737 kb on - strand, within Pf6N2E2_169at 221.737 kb on - strand, within Pf6N2E2_169at 221.737 kb on - strand, within Pf6N2E2_169at 221.817 kb on + strand, within Pf6N2E2_169at 221.865 kb on + strand, within Pf6N2E2_169at 221.865 kb on + strand, within Pf6N2E2_169at 221.865 kb on + strand, within Pf6N2E2_169at 221.865 kb on + strand, within Pf6N2E2_169at 221.865 kb on + strand, within Pf6N2E2_169at 221.866 kb on - strand, within Pf6N2E2_169at 221.866 kb on - strand, within Pf6N2E2_169at 221.866 kb on - strand, within Pf6N2E2_169at 221.866 kb on - strand, within Pf6N2E2_169at 221.866 kb on - strand, within Pf6N2E2_169at 221.866 kb on - strand, within Pf6N2E2_169at 221.866 kb on - strand, within Pf6N2E2_169at 221.866 kb on - strand, within Pf6N2E2_169at 221.882 kb on + strand, within Pf6N2E2_169at 221.882 kb on + strand, within Pf6N2E2_169at 221.882 kb on + strand, within Pf6N2E2_169at 221.883 kb on - strand, within Pf6N2E2_169at 221.883 kb on - strand, within Pf6N2E2_169at 221.907 kb on + strandat 221.907 kb on + strandat 221.920 kb on - strandat 222.063 kb on + strandat 222.063 kb on + strandat 222.063 kb on + strandat 222.064 kb on - strandat 222.113 kb on + strandat 222.113 kb on + strandat 222.113 kb on + strandat 222.114 kb on - strandat 222.114 kb on - strandat 222.359 kb on + strand, within Pf6N2E2_170at 222.360 kb on - strand, within Pf6N2E2_170at 222.455 kb on + strandat 222.472 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml
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220,393 - Pf6N2E2_167 0.17 +0.9
220,393 - Pf6N2E2_167 0.17 +0.1
220,393 - Pf6N2E2_167 0.17 +1.8
220,393 - Pf6N2E2_167 0.17 +0.6
220,495 + Pf6N2E2_167 0.27 -0.7
220,496 - Pf6N2E2_167 0.27 -1.6
220,580 + Pf6N2E2_167 0.35 +1.2
220,581 - Pf6N2E2_167 0.35 -2.1
220,581 - Pf6N2E2_167 0.35 -0.0
220,624 + Pf6N2E2_167 0.40 +0.3
220,785 + Pf6N2E2_167 0.55 +0.4
220,785 + Pf6N2E2_167 0.55 +1.2
220,867 + Pf6N2E2_167 0.63 +0.3
220,978 + Pf6N2E2_167 0.74 -0.0
220,978 + Pf6N2E2_167 0.74 -0.7
220,979 - Pf6N2E2_167 0.74 +0.4
220,979 - Pf6N2E2_167 0.74 -1.3
220,979 - Pf6N2E2_167 0.74 -0.9
220,979 - Pf6N2E2_167 0.74 -0.2
220,979 - Pf6N2E2_167 0.74 +1.0
220,979 - Pf6N2E2_167 0.74 +0.4
221,002 + Pf6N2E2_167 0.76 -0.0
221,002 + Pf6N2E2_167 0.76 -0.6
221,144 - -0.4
221,144 - -0.6
221,255 + +0.4
221,255 + -0.1
221,256 - +1.8
221,256 - +0.1
221,257 + -1.1
221,258 - +1.0
221,258 - +0.1
221,389 + Pf6N2E2_168 0.48 -0.5
221,389 + Pf6N2E2_168 0.48 -0.9
221,429 + Pf6N2E2_168 0.69 -0.5
221,429 + Pf6N2E2_168 0.69 -0.6
221,429 + Pf6N2E2_168 0.69 -1.6
221,430 - Pf6N2E2_168 0.69 -0.8
221,430 - Pf6N2E2_168 0.69 +0.2
221,430 - Pf6N2E2_168 0.69 -1.8
221,436 + Pf6N2E2_168 0.72 +0.3
221,436 + Pf6N2E2_168 0.72 -0.9
221,437 - Pf6N2E2_168 0.73 -2.1
221,493 + -1.7
221,493 + -2.7
221,494 - +0.7
221,517 + -0.0
221,517 + -1.3
221,517 + -1.0
221,518 - +1.1
221,518 - +1.9
221,518 - -1.1
221,518 - -0.5
221,527 + -0.7
221,528 - +0.5
221,561 + -2.2
221,562 - -1.4
221,562 - +0.1
221,702 + Pf6N2E2_169 0.27 +0.0
221,703 - Pf6N2E2_169 0.27 +0.6
221,736 + Pf6N2E2_169 0.38 -0.0
221,737 - Pf6N2E2_169 0.39 +0.4
221,737 - Pf6N2E2_169 0.39 -0.9
221,737 - Pf6N2E2_169 0.39 +1.6
221,737 - Pf6N2E2_169 0.39 -0.2
221,817 + Pf6N2E2_169 0.65 +0.8
221,865 + Pf6N2E2_169 0.80 -0.5
221,865 + Pf6N2E2_169 0.80 -0.5
221,865 + Pf6N2E2_169 0.80 +0.9
221,865 + Pf6N2E2_169 0.80 -0.5
221,865 + Pf6N2E2_169 0.80 -0.7
221,866 - Pf6N2E2_169 0.81 -0.6
221,866 - Pf6N2E2_169 0.81 +0.9
221,866 - Pf6N2E2_169 0.81 -1.4
221,866 - Pf6N2E2_169 0.81 +0.9
221,866 - Pf6N2E2_169 0.81 -0.1
221,866 - Pf6N2E2_169 0.81 +0.6
221,866 - Pf6N2E2_169 0.81 +0.3
221,866 - Pf6N2E2_169 0.81 -0.8
221,882 + Pf6N2E2_169 0.86 +1.5
221,882 + Pf6N2E2_169 0.86 -0.2
221,882 + Pf6N2E2_169 0.86 -1.8
221,883 - Pf6N2E2_169 0.86 -0.6
221,883 - Pf6N2E2_169 0.86 +1.5
221,907 + -1.2
221,907 + -0.6
221,920 - +0.5
222,063 + -1.8
222,063 + -1.2
222,063 + -0.7
222,064 - +0.2
222,113 + +0.3
222,113 + +0.5
222,113 + -0.1
222,114 - -0.1
222,114 - -0.1
222,359 + Pf6N2E2_170 0.86 -0.2
222,360 - Pf6N2E2_170 0.87 -0.4
222,455 + -3.2
222,472 - -1.2

Or see this region's nucleotide sequence