Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1491 and Pf6N2E2_1492 are separated by 45 nucleotides Pf6N2E2_1492 and Pf6N2E2_1493 are separated by 27 nucleotides Pf6N2E2_1493 and Pf6N2E2_1494 overlap by 14 nucleotides Pf6N2E2_1494 and Pf6N2E2_1495 overlap by 11 nucleotides
Pf6N2E2_1491 - Cystathionine beta-lyase (EC 4.4.1.8), at 1,743,712 to 1,746,477
_1491
Pf6N2E2_1492 - No significant database matches, at 1,746,523 to 1,747,122
_1492
Pf6N2E2_1493 - ABC-type polar amino acid transport system, ATPase component, at 1,747,150 to 1,747,986
_1493
Pf6N2E2_1494 - Glutamate transport membrane-spanning protein, at 1,747,973 to 1,748,761
_1494
Pf6N2E2_1495 - Glutamate transport membrane-spanning protein, at 1,748,751 to 1,749,485
_1495
Position (kb) 1747
1748 Strain fitness (log2 ratio) -2
-1
0
1 at 1746.346 kb on - strand at 1746.463 kb on + strand at 1746.464 kb on - strand at 1746.534 kb on - strand at 1746.569 kb on + strand at 1746.570 kb on - strand at 1746.636 kb on - strand, within Pf6N2E2_1492 at 1746.715 kb on - strand, within Pf6N2E2_1492 at 1746.715 kb on - strand, within Pf6N2E2_1492 at 1746.758 kb on + strand, within Pf6N2E2_1492 at 1746.792 kb on - strand, within Pf6N2E2_1492 at 1747.117 kb on - strand at 1747.191 kb on - strand at 1747.191 kb on - strand at 1747.191 kb on - strand at 1747.243 kb on + strand, within Pf6N2E2_1493 at 1747.332 kb on - strand, within Pf6N2E2_1493 at 1747.382 kb on - strand, within Pf6N2E2_1493 at 1747.694 kb on + strand, within Pf6N2E2_1493 at 1747.694 kb on + strand, within Pf6N2E2_1493 at 1747.694 kb on + strand, within Pf6N2E2_1493 at 1747.694 kb on + strand, within Pf6N2E2_1493 at 1747.695 kb on - strand, within Pf6N2E2_1493 at 1747.695 kb on - strand, within Pf6N2E2_1493 at 1747.977 kb on + strand at 1748.008 kb on + strand at 1748.009 kb on - strand at 1748.031 kb on + strand at 1748.062 kb on - strand, within Pf6N2E2_1494 at 1748.062 kb on - strand, within Pf6N2E2_1494 at 1748.179 kb on - strand, within Pf6N2E2_1494 at 1748.179 kb on - strand, within Pf6N2E2_1494 at 1748.427 kb on + strand, within Pf6N2E2_1494 at 1748.427 kb on + strand, within Pf6N2E2_1494 at 1748.427 kb on + strand, within Pf6N2E2_1494 at 1748.427 kb on + strand, within Pf6N2E2_1494 at 1748.427 kb on + strand, within Pf6N2E2_1494 at 1748.427 kb on + strand, within Pf6N2E2_1494 at 1748.428 kb on - strand, within Pf6N2E2_1494 at 1748.428 kb on - strand, within Pf6N2E2_1494 at 1748.428 kb on - strand, within Pf6N2E2_1494 at 1748.439 kb on + strand, within Pf6N2E2_1494 at 1748.665 kb on - strand, within Pf6N2E2_1494 at 1748.665 kb on - strand, within Pf6N2E2_1494 at 1748.716 kb on + strand at 1748.899 kb on + strand, within Pf6N2E2_1495 at 1748.899 kb on + strand, within Pf6N2E2_1495 at 1748.900 kb on - strand, within Pf6N2E2_1495 at 1748.900 kb on - strand, within Pf6N2E2_1495 at 1748.900 kb on - strand, within Pf6N2E2_1495
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 1,746,346 - -0.2 1,746,463 + -1.2 1,746,464 - -0.8 1,746,534 - +0.5 1,746,569 + +1.6 1,746,570 - -0.9 1,746,636 - Pf6N2E2_1492 0.19 +1.3 1,746,715 - Pf6N2E2_1492 0.32 -0.4 1,746,715 - Pf6N2E2_1492 0.32 -1.3 1,746,758 + Pf6N2E2_1492 0.39 -0.1 1,746,792 - Pf6N2E2_1492 0.45 -0.3 1,747,117 - -1.1 1,747,191 - -0.3 1,747,191 - -0.3 1,747,191 - -0.6 1,747,243 + Pf6N2E2_1493 0.11 -0.9 1,747,332 - Pf6N2E2_1493 0.22 -1.9 1,747,382 - Pf6N2E2_1493 0.28 -0.9 1,747,694 + Pf6N2E2_1493 0.65 -0.4 1,747,694 + Pf6N2E2_1493 0.65 +0.7 1,747,694 + Pf6N2E2_1493 0.65 -1.0 1,747,694 + Pf6N2E2_1493 0.65 -0.4 1,747,695 - Pf6N2E2_1493 0.65 -0.3 1,747,695 - Pf6N2E2_1493 0.65 -1.6 1,747,977 + -0.8 1,748,008 + +1.4 1,748,009 - +0.3 1,748,031 + +0.8 1,748,062 - Pf6N2E2_1494 0.11 -1.0 1,748,062 - Pf6N2E2_1494 0.11 -0.2 1,748,179 - Pf6N2E2_1494 0.26 +0.7 1,748,179 - Pf6N2E2_1494 0.26 -0.5 1,748,427 + Pf6N2E2_1494 0.58 -1.1 1,748,427 + Pf6N2E2_1494 0.58 +0.1 1,748,427 + Pf6N2E2_1494 0.58 +1.2 1,748,427 + Pf6N2E2_1494 0.58 +0.4 1,748,427 + Pf6N2E2_1494 0.58 +0.3 1,748,427 + Pf6N2E2_1494 0.58 -2.2 1,748,428 - Pf6N2E2_1494 0.58 -0.5 1,748,428 - Pf6N2E2_1494 0.58 -1.4 1,748,428 - Pf6N2E2_1494 0.58 +0.3 1,748,439 + Pf6N2E2_1494 0.59 -0.5 1,748,665 - Pf6N2E2_1494 0.88 +0.0 1,748,665 - Pf6N2E2_1494 0.88 +0.1 1,748,716 + -0.7 1,748,899 + Pf6N2E2_1495 0.20 +0.9 1,748,899 + Pf6N2E2_1495 0.20 +1.6 1,748,900 - Pf6N2E2_1495 0.20 -0.1 1,748,900 - Pf6N2E2_1495 0.20 +0.9 1,748,900 - Pf6N2E2_1495 0.20 +1.0
Or see this region's nucleotide sequence