Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1434 and Pf6N2E2_1435 are separated by 14 nucleotides Pf6N2E2_1435 and Pf6N2E2_1436 are separated by 40 nucleotides Pf6N2E2_1436 and Pf6N2E2_1437 are separated by 377 nucleotides
Pf6N2E2_1434 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 1,683,522 to 1,684,682
_1434
Pf6N2E2_1435 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases; sulfonate monooxygenase, at 1,684,697 to 1,685,794
_1435
Pf6N2E2_1436 - FMN reductase (EC 1.5.1.29), at 1,685,835 to 1,686,398
_1436
Pf6N2E2_1437 - Acyl-CoA dehydrogenase family protein, at 1,686,776 to 1,688,020
_1437
Position (kb)
1684
1685
1686 Strain fitness (log2 ratio)
-1
0
1 at 1683.841 kb on + strand, within Pf6N2E2_1434 at 1683.860 kb on - strand, within Pf6N2E2_1434 at 1683.860 kb on - strand, within Pf6N2E2_1434 at 1684.262 kb on - strand, within Pf6N2E2_1434 at 1684.581 kb on - strand at 1684.581 kb on - strand at 1684.581 kb on - strand at 1684.698 kb on + strand at 1684.777 kb on - strand at 1685.163 kb on - strand, within Pf6N2E2_1435 at 1685.185 kb on - strand, within Pf6N2E2_1435 at 1685.284 kb on - strand, within Pf6N2E2_1435 at 1685.649 kb on + strand, within Pf6N2E2_1435 at 1685.694 kb on + strand at 1685.694 kb on + strand at 1685.695 kb on - strand at 1685.695 kb on - strand at 1685.766 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.801 kb on + strand at 1685.802 kb on - strand at 1685.802 kb on - strand at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1686.079 kb on - strand, within Pf6N2E2_1436 at 1686.137 kb on - strand, within Pf6N2E2_1436 at 1686.137 kb on - strand, within Pf6N2E2_1436 at 1686.149 kb on - strand, within Pf6N2E2_1436 at 1686.447 kb on - strand at 1686.483 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 1,683,841 + Pf6N2E2_1434 0.27 +0.7 1,683,860 - Pf6N2E2_1434 0.29 -0.9 1,683,860 - Pf6N2E2_1434 0.29 -1.4 1,684,262 - Pf6N2E2_1434 0.64 -0.3 1,684,581 - +1.2 1,684,581 - +1.0 1,684,581 - -0.5 1,684,698 + +0.0 1,684,777 - -0.3 1,685,163 - Pf6N2E2_1435 0.42 -0.1 1,685,185 - Pf6N2E2_1435 0.44 -1.5 1,685,284 - Pf6N2E2_1435 0.53 -0.9 1,685,649 + Pf6N2E2_1435 0.87 -0.4 1,685,694 + +0.2 1,685,694 + +0.8 1,685,695 - +0.3 1,685,695 - -0.7 1,685,766 + -0.2 1,685,795 + -1.0 1,685,795 + -0.7 1,685,795 + +0.4 1,685,795 + -0.6 1,685,795 + -0.2 1,685,795 + +0.4 1,685,795 + +0.3 1,685,795 + -1.5 1,685,795 + +0.2 1,685,795 + -0.2 1,685,795 + +0.5 1,685,795 + -0.6 1,685,795 + -0.0 1,685,796 - +0.1 1,685,796 - +1.3 1,685,796 - -0.6 1,685,796 - -0.5 1,685,796 - -1.0 1,685,796 - +0.3 1,685,796 - +0.5 1,685,801 + -1.1 1,685,802 - -1.7 1,685,802 - +0.8 1,685,965 + Pf6N2E2_1436 0.23 -1.3 1,685,965 + Pf6N2E2_1436 0.23 -0.6 1,685,965 + Pf6N2E2_1436 0.23 -0.5 1,685,965 + Pf6N2E2_1436 0.23 -0.3 1,685,965 + Pf6N2E2_1436 0.23 -0.8 1,686,079 - Pf6N2E2_1436 0.43 +0.6 1,686,137 - Pf6N2E2_1436 0.54 -1.1 1,686,137 - Pf6N2E2_1436 0.54 -1.4 1,686,149 - Pf6N2E2_1436 0.56 -0.3 1,686,447 - +0.8 1,686,483 - +0.1
Or see this region's nucleotide sequence