Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_132 and Pf6N2E2_133 are separated by 42 nucleotides Pf6N2E2_133 and Pf6N2E2_134 are separated by 342 nucleotides Pf6N2E2_134 and Pf6N2E2_135 are separated by 88 nucleotides
Pf6N2E2_132 - hcp, at 181,377 to 181,658
_132
Pf6N2E2_133 - hypothetical protein, at 181,701 to 181,850
_133
Pf6N2E2_134 - FIG00960493: hypothetical protein, at 182,193 to 183,569
_134
Pf6N2E2_135 - Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF, at 183,658 to 184,878
_135
Position (kb) 182
183
184 Strain fitness (log2 ratio) -2
-1
0
1
2 at 181.205 kb on - strand at 181.221 kb on + strand at 181.221 kb on + strand at 181.221 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.225 kb on - strand at 181.225 kb on - strand at 181.225 kb on - strand at 181.225 kb on - strand at 181.255 kb on + strand at 181.256 kb on - strand at 181.312 kb on - strand at 181.321 kb on + strand at 181.321 kb on + strand at 181.428 kb on + strand, within Pf6N2E2_132 at 181.428 kb on + strand, within Pf6N2E2_132 at 181.429 kb on - strand, within Pf6N2E2_132 at 181.429 kb on - strand, within Pf6N2E2_132 at 181.429 kb on - strand, within Pf6N2E2_132 at 181.519 kb on + strand, within Pf6N2E2_132 at 181.519 kb on + strand, within Pf6N2E2_132 at 181.564 kb on + strand, within Pf6N2E2_132 at 181.565 kb on - strand, within Pf6N2E2_132 at 181.565 kb on - strand, within Pf6N2E2_132 at 181.567 kb on + strand, within Pf6N2E2_132 at 181.567 kb on + strand, within Pf6N2E2_132 at 181.567 kb on + strand, within Pf6N2E2_132 at 181.568 kb on - strand, within Pf6N2E2_132 at 181.568 kb on - strand, within Pf6N2E2_132 at 181.568 kb on - strand, within Pf6N2E2_132 at 181.683 kb on - strand at 181.692 kb on + strand at 181.693 kb on - strand at 181.693 kb on - strand at 181.705 kb on - strand at 181.705 kb on - strand at 181.790 kb on + strand, within Pf6N2E2_133 at 181.811 kb on + strand, within Pf6N2E2_133 at 181.829 kb on + strand, within Pf6N2E2_133 at 181.851 kb on + strand at 181.851 kb on + strand at 181.851 kb on + strand at 181.851 kb on + strand at 181.852 kb on - strand at 181.852 kb on - strand at 181.852 kb on - strand at 181.852 kb on - strand at 181.852 kb on - strand at 181.879 kb on + strand at 181.879 kb on + strand at 181.879 kb on + strand at 181.880 kb on - strand at 182.188 kb on + strand at 182.320 kb on + strand at 182.320 kb on + strand at 182.392 kb on + strand, within Pf6N2E2_134 at 182.392 kb on + strand, within Pf6N2E2_134 at 182.393 kb on - strand, within Pf6N2E2_134 at 182.393 kb on - strand, within Pf6N2E2_134 at 182.416 kb on + strand, within Pf6N2E2_134 at 182.416 kb on + strand, within Pf6N2E2_134 at 182.417 kb on - strand, within Pf6N2E2_134 at 182.506 kb on + strand, within Pf6N2E2_134 at 182.506 kb on + strand, within Pf6N2E2_134 at 182.506 kb on + strand, within Pf6N2E2_134 at 182.506 kb on + strand, within Pf6N2E2_134 at 182.581 kb on + strand, within Pf6N2E2_134 at 182.582 kb on - strand, within Pf6N2E2_134 at 182.582 kb on - strand, within Pf6N2E2_134 at 183.027 kb on + strand, within Pf6N2E2_134 at 183.071 kb on - strand, within Pf6N2E2_134 at 183.192 kb on - strand, within Pf6N2E2_134 at 183.283 kb on + strand, within Pf6N2E2_134 at 183.283 kb on + strand, within Pf6N2E2_134 at 183.283 kb on + strand, within Pf6N2E2_134 at 183.284 kb on - strand, within Pf6N2E2_134 at 183.284 kb on - strand, within Pf6N2E2_134 at 183.284 kb on - strand, within Pf6N2E2_134 at 183.487 kb on + strand at 183.487 kb on + strand at 183.508 kb on + strand at 183.508 kb on + strand at 183.508 kb on + strand at 183.509 kb on - strand at 183.509 kb on - strand at 183.509 kb on - strand at 183.528 kb on - strand at 183.839 kb on + strand, within Pf6N2E2_135 at 183.839 kb on + strand, within Pf6N2E2_135 at 183.839 kb on + strand, within Pf6N2E2_135 at 183.839 kb on + strand, within Pf6N2E2_135 at 183.840 kb on - strand, within Pf6N2E2_135 at 184.106 kb on + strand, within Pf6N2E2_135 at 184.239 kb on - strand, within Pf6N2E2_135
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 181,205 - -0.5 181,221 + -0.1 181,221 + +0.0 181,221 + -0.7 181,224 + -0.2 181,224 + -0.0 181,224 + -1.7 181,224 + -0.9 181,224 + +0.8 181,224 + +0.6 181,224 + -0.2 181,224 + -0.6 181,225 - +1.1 181,225 - -1.0 181,225 - -0.3 181,225 - +1.3 181,255 + +0.8 181,256 - +0.1 181,312 - +2.3 181,321 + +1.9 181,321 + +0.9 181,428 + Pf6N2E2_132 0.18 +1.5 181,428 + Pf6N2E2_132 0.18 -0.3 181,429 - Pf6N2E2_132 0.18 -0.9 181,429 - Pf6N2E2_132 0.18 -1.9 181,429 - Pf6N2E2_132 0.18 +1.1 181,519 + Pf6N2E2_132 0.50 -0.7 181,519 + Pf6N2E2_132 0.50 -2.7 181,564 + Pf6N2E2_132 0.66 -0.5 181,565 - Pf6N2E2_132 0.67 +0.1 181,565 - Pf6N2E2_132 0.67 -0.0 181,567 + Pf6N2E2_132 0.67 -0.1 181,567 + Pf6N2E2_132 0.67 -1.9 181,567 + Pf6N2E2_132 0.67 -0.9 181,568 - Pf6N2E2_132 0.68 -0.6 181,568 - Pf6N2E2_132 0.68 +0.2 181,568 - Pf6N2E2_132 0.68 -1.5 181,683 - -0.6 181,692 + -0.4 181,693 - +0.2 181,693 - -0.0 181,705 - -0.7 181,705 - +0.5 181,790 + Pf6N2E2_133 0.59 -0.5 181,811 + Pf6N2E2_133 0.73 -1.5 181,829 + Pf6N2E2_133 0.85 -0.4 181,851 + -0.3 181,851 + -1.3 181,851 + -0.8 181,851 + -0.1 181,852 - -0.1 181,852 - -1.0 181,852 - +0.2 181,852 - -0.1 181,852 - -0.4 181,879 + -1.8 181,879 + -0.5 181,879 + +0.0 181,880 - -0.1 182,188 + -0.7 182,320 + -0.8 182,320 + -0.3 182,392 + Pf6N2E2_134 0.14 -1.2 182,392 + Pf6N2E2_134 0.14 -0.9 182,393 - Pf6N2E2_134 0.15 -1.4 182,393 - Pf6N2E2_134 0.15 -0.3 182,416 + Pf6N2E2_134 0.16 -1.4 182,416 + Pf6N2E2_134 0.16 -0.5 182,417 - Pf6N2E2_134 0.16 +0.5 182,506 + Pf6N2E2_134 0.23 -0.0 182,506 + Pf6N2E2_134 0.23 -1.9 182,506 + Pf6N2E2_134 0.23 -0.4 182,506 + Pf6N2E2_134 0.23 -2.0 182,581 + Pf6N2E2_134 0.28 +0.5 182,582 - Pf6N2E2_134 0.28 -1.2 182,582 - Pf6N2E2_134 0.28 +0.3 183,027 + Pf6N2E2_134 0.61 -0.5 183,071 - Pf6N2E2_134 0.64 -1.3 183,192 - Pf6N2E2_134 0.73 -1.7 183,283 + Pf6N2E2_134 0.79 +0.4 183,283 + Pf6N2E2_134 0.79 +0.1 183,283 + Pf6N2E2_134 0.79 -0.8 183,284 - Pf6N2E2_134 0.79 +0.3 183,284 - Pf6N2E2_134 0.79 +0.3 183,284 - Pf6N2E2_134 0.79 -0.2 183,487 + -1.5 183,487 + -1.1 183,508 + -0.3 183,508 + -1.0 183,508 + -0.0 183,509 - -1.1 183,509 - +0.5 183,509 - -0.2 183,528 - +0.7 183,839 + Pf6N2E2_135 0.15 -0.0 183,839 + Pf6N2E2_135 0.15 +0.3 183,839 + Pf6N2E2_135 0.15 -0.7 183,839 + Pf6N2E2_135 0.15 +0.0 183,840 - Pf6N2E2_135 0.15 -0.3 184,106 + Pf6N2E2_135 0.37 -1.3 184,239 - Pf6N2E2_135 0.48 +0.4
Or see this region's nucleotide sequence