Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1324 and Pf6N2E2_1325 are separated by 123 nucleotides Pf6N2E2_1325 and Pf6N2E2_1326 overlap by 41 nucleotides
Pf6N2E2_1324 - Transcriptional regulator, AraC family, at 1,557,386 to 1,558,348
_1324
Pf6N2E2_1325 - FIG00954773: hypothetical protein, at 1,558,472 to 1,559,077
_1325
Pf6N2E2_1326 - Cationic amino acid transporter, at 1,559,037 to 1,560,536
_1326
Position (kb)
1558
1559
1560 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1557.515 kb on - strand, within Pf6N2E2_1324 at 1557.515 kb on - strand, within Pf6N2E2_1324 at 1557.584 kb on - strand, within Pf6N2E2_1324 at 1557.586 kb on - strand, within Pf6N2E2_1324 at 1557.713 kb on + strand, within Pf6N2E2_1324 at 1557.713 kb on + strand, within Pf6N2E2_1324 at 1557.713 kb on + strand, within Pf6N2E2_1324 at 1557.713 kb on + strand, within Pf6N2E2_1324 at 1557.714 kb on - strand, within Pf6N2E2_1324 at 1557.714 kb on - strand, within Pf6N2E2_1324 at 1557.714 kb on - strand, within Pf6N2E2_1324 at 1557.714 kb on - strand, within Pf6N2E2_1324 at 1557.717 kb on + strand, within Pf6N2E2_1324 at 1557.718 kb on - strand, within Pf6N2E2_1324 at 1557.718 kb on - strand, within Pf6N2E2_1324 at 1557.718 kb on - strand, within Pf6N2E2_1324 at 1557.718 kb on - strand, within Pf6N2E2_1324 at 1557.718 kb on - strand, within Pf6N2E2_1324 at 1557.770 kb on - strand, within Pf6N2E2_1324 at 1558.098 kb on + strand, within Pf6N2E2_1324 at 1558.157 kb on + strand, within Pf6N2E2_1324 at 1558.369 kb on - strand at 1558.369 kb on - strand at 1558.403 kb on + strand at 1558.404 kb on - strand at 1558.421 kb on - strand at 1558.551 kb on + strand, within Pf6N2E2_1325 at 1558.552 kb on - strand, within Pf6N2E2_1325 at 1558.552 kb on - strand, within Pf6N2E2_1325 at 1558.756 kb on + strand, within Pf6N2E2_1325 at 1559.218 kb on + strand, within Pf6N2E2_1326 at 1559.218 kb on + strand, within Pf6N2E2_1326 at 1559.218 kb on + strand, within Pf6N2E2_1326 at 1559.218 kb on + strand, within Pf6N2E2_1326 at 1559.219 kb on - strand, within Pf6N2E2_1326 at 1559.219 kb on - strand, within Pf6N2E2_1326 at 1559.527 kb on + strand, within Pf6N2E2_1326 at 1559.528 kb on - strand, within Pf6N2E2_1326 at 1559.528 kb on - strand, within Pf6N2E2_1326 at 1559.570 kb on - strand, within Pf6N2E2_1326
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 1,557,515 - Pf6N2E2_1324 0.13 -1.5 1,557,515 - Pf6N2E2_1324 0.13 -0.1 1,557,584 - Pf6N2E2_1324 0.21 -1.4 1,557,586 - Pf6N2E2_1324 0.21 -2.7 1,557,713 + Pf6N2E2_1324 0.34 -1.1 1,557,713 + Pf6N2E2_1324 0.34 -1.2 1,557,713 + Pf6N2E2_1324 0.34 +0.3 1,557,713 + Pf6N2E2_1324 0.34 -0.9 1,557,714 - Pf6N2E2_1324 0.34 -0.4 1,557,714 - Pf6N2E2_1324 0.34 +0.5 1,557,714 - Pf6N2E2_1324 0.34 +0.5 1,557,714 - Pf6N2E2_1324 0.34 -1.3 1,557,717 + Pf6N2E2_1324 0.34 -1.6 1,557,718 - Pf6N2E2_1324 0.34 +0.3 1,557,718 - Pf6N2E2_1324 0.34 -0.5 1,557,718 - Pf6N2E2_1324 0.34 +1.5 1,557,718 - Pf6N2E2_1324 0.34 +0.4 1,557,718 - Pf6N2E2_1324 0.34 -2.0 1,557,770 - Pf6N2E2_1324 0.40 -0.3 1,558,098 + Pf6N2E2_1324 0.74 +0.7 1,558,157 + Pf6N2E2_1324 0.80 -0.8 1,558,369 - -0.7 1,558,369 - -0.2 1,558,403 + -0.2 1,558,404 - +0.5 1,558,421 - -0.0 1,558,551 + Pf6N2E2_1325 0.13 -0.7 1,558,552 - Pf6N2E2_1325 0.13 -0.8 1,558,552 - Pf6N2E2_1325 0.13 -2.2 1,558,756 + Pf6N2E2_1325 0.47 -0.5 1,559,218 + Pf6N2E2_1326 0.12 -0.0 1,559,218 + Pf6N2E2_1326 0.12 -1.3 1,559,218 + Pf6N2E2_1326 0.12 +0.2 1,559,218 + Pf6N2E2_1326 0.12 +1.0 1,559,219 - Pf6N2E2_1326 0.12 -0.6 1,559,219 - Pf6N2E2_1326 0.12 -0.6 1,559,527 + Pf6N2E2_1326 0.33 -2.8 1,559,528 - Pf6N2E2_1326 0.33 -0.7 1,559,528 - Pf6N2E2_1326 0.33 -1.7 1,559,570 - Pf6N2E2_1326 0.36 +0.8
Or see this region's nucleotide sequence