Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1302 and Pf6N2E2_1303 overlap by 4 nucleotides Pf6N2E2_1303 and Pf6N2E2_1304 are separated by 489 nucleotides Pf6N2E2_1304 and Pf6N2E2_1305 are separated by 171 nucleotides
Pf6N2E2_1302 - TRAP-type C4-dicarboxylate transport system, large permease component, at 1,537,147 to 1,538,427
_1302
Pf6N2E2_1303 - TRAP-type transport system, small permease component, predicted N-acetylneuraminate transporter, at 1,538,424 to 1,538,984
_1303
Pf6N2E2_1304 - Cell division transporter, ATP-binding protein FtsE (TC 3.A.5.1.1), at 1,539,474 to 1,539,722
_1304
Pf6N2E2_1305 - Homoprotocatechuate degradative operon repressor, at 1,539,894 to 1,540,295
_1305
Position (kb)
1538
1539 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1537.634 kb on + strand, within Pf6N2E2_1302 at 1537.634 kb on + strand, within Pf6N2E2_1302 at 1537.635 kb on - strand, within Pf6N2E2_1302 at 1537.635 kb on - strand, within Pf6N2E2_1302 at 1537.856 kb on + strand, within Pf6N2E2_1302 at 1537.856 kb on + strand, within Pf6N2E2_1302 at 1537.856 kb on + strand, within Pf6N2E2_1302 at 1537.856 kb on + strand, within Pf6N2E2_1302 at 1537.856 kb on + strand, within Pf6N2E2_1302 at 1537.857 kb on - strand, within Pf6N2E2_1302 at 1538.145 kb on - strand, within Pf6N2E2_1302 at 1538.163 kb on + strand, within Pf6N2E2_1302 at 1538.415 kb on + strand at 1538.436 kb on - strand at 1538.444 kb on + strand at 1538.445 kb on - strand at 1538.600 kb on - strand, within Pf6N2E2_1303 at 1538.600 kb on - strand, within Pf6N2E2_1303 at 1538.695 kb on + strand, within Pf6N2E2_1303 at 1538.696 kb on - strand, within Pf6N2E2_1303 at 1538.696 kb on - strand, within Pf6N2E2_1303 at 1538.730 kb on - strand, within Pf6N2E2_1303 at 1538.931 kb on + strand at 1538.932 kb on - strand at 1538.932 kb on - strand at 1539.023 kb on - strand at 1539.061 kb on - strand at 1539.150 kb on - strand at 1539.155 kb on + strand at 1539.156 kb on - strand at 1539.158 kb on + strand at 1539.159 kb on - strand at 1539.318 kb on - strand at 1539.327 kb on + strand at 1539.327 kb on + strand at 1539.389 kb on + strand at 1539.389 kb on + strand at 1539.980 kb on - strand, within Pf6N2E2_1305
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 1,537,634 + Pf6N2E2_1302 0.38 -0.1 1,537,634 + Pf6N2E2_1302 0.38 -0.6 1,537,635 - Pf6N2E2_1302 0.38 -1.0 1,537,635 - Pf6N2E2_1302 0.38 -0.9 1,537,856 + Pf6N2E2_1302 0.55 -0.3 1,537,856 + Pf6N2E2_1302 0.55 -0.4 1,537,856 + Pf6N2E2_1302 0.55 +2.0 1,537,856 + Pf6N2E2_1302 0.55 -0.0 1,537,856 + Pf6N2E2_1302 0.55 +1.2 1,537,857 - Pf6N2E2_1302 0.55 +2.6 1,538,145 - Pf6N2E2_1302 0.78 -0.4 1,538,163 + Pf6N2E2_1302 0.79 +1.4 1,538,415 + +0.0 1,538,436 - -1.7 1,538,444 + -1.1 1,538,445 - +0.5 1,538,600 - Pf6N2E2_1303 0.31 -1.9 1,538,600 - Pf6N2E2_1303 0.31 -2.4 1,538,695 + Pf6N2E2_1303 0.48 -0.4 1,538,696 - Pf6N2E2_1303 0.48 +0.7 1,538,696 - Pf6N2E2_1303 0.48 -1.1 1,538,730 - Pf6N2E2_1303 0.55 -0.6 1,538,931 + -0.8 1,538,932 - -0.4 1,538,932 - -0.8 1,539,023 - -0.1 1,539,061 - -2.3 1,539,150 - -0.3 1,539,155 + -1.4 1,539,156 - -1.0 1,539,158 + -0.0 1,539,159 - +1.2 1,539,318 - -0.3 1,539,327 + -0.1 1,539,327 + +0.3 1,539,389 + -0.6 1,539,389 + +1.4 1,539,980 - Pf6N2E2_1305 0.21 -0.1
Or see this region's nucleotide sequence